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Translational biophotonics最新文献

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Using micro and nanofluidics with surface enhanced Raman spectroscopy for in vitro blood based biomarker detection 微纳米流体表面增强拉曼光谱技术在体外血液生物标志物检测中的应用
Translational biophotonics Pub Date : 2014-05-19 DOI: 10.1117/12.2065418
G. Coté, J. Kameoka, Haley L. Marks
{"title":"Using micro and nanofluidics with surface enhanced Raman spectroscopy for in vitro blood based biomarker detection","authors":"G. Coté, J. Kameoka, Haley L. Marks","doi":"10.1117/12.2065418","DOIUrl":"https://doi.org/10.1117/12.2065418","url":null,"abstract":"In this presentation we will discuss the development of a point-of-care optofluidic device that uses gold nanoparticlebased surface enhanced Raman spectroscopy (SERS) for detection of blood biomarkers. SERS approaches have been successfully used for detection of analytes due to the large enhancements provided by the interaction between the light, gold particles, and analyte. However, SERS approaches developed for use to accurately quantify an analyte have suffered from a lack of repeatability. We will describe our SERS optofluidic device with functionalized nanoparticles that helps to overcome these problems and will show results with a focus on blood cardiac biomarkers.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"63 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"80318460","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Wide-field endoscopic fluorescence imaging for gastrointestinal tumor detection with glucose analogue 葡萄糖类似物用于胃肠道肿瘤检测的宽视场内镜荧光成像
Translational biophotonics Pub Date : 2014-05-19 DOI: 10.1117/12.2057696
Yun He, Y. Qu, Jing Bai, Hai-feng Liu
{"title":"Wide-field endoscopic fluorescence imaging for gastrointestinal tumor detection with glucose analogue","authors":"Yun He, Y. Qu, Jing Bai, Hai-feng Liu","doi":"10.1117/12.2057696","DOIUrl":"https://doi.org/10.1117/12.2057696","url":null,"abstract":"The lack of functional information and targeted imaging in conventional white-light endoscopy leads to a high miss-rate of gastrointestinal tumor. The combination of near-infrared fluorescence imaging and endoscopy presents a promising approach. Here we introduce a new endoscopy method employing a home-made flexible wide-field epi-fluorescence endoscope, that can be inserted through the biopsy channel of a gastrointestinal endoscope, with the glucose analogue 2- DeoxyGlucosone as the near-infrared fluorescent probe. System characterization indicates a good sensitivity and linearity over a large field of view. Its capability of tumor identification and location is demonstrated with in-vivo imaging of xenografted tumor model.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88878918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Assessing lymphatic response to treatments in head and neck cancer using near-infrared fluorescence imaging 使用近红外荧光成像评估头颈癌治疗的淋巴反应
Translational biophotonics Pub Date : 2014-05-19 DOI: 10.1117/12.2057788
I. Tan, R. Karni, J. Rasmussen, E. Sevick-Muraca
{"title":"Assessing lymphatic response to treatments in head and neck cancer using near-infrared fluorescence imaging","authors":"I. Tan, R. Karni, J. Rasmussen, E. Sevick-Muraca","doi":"10.1117/12.2057788","DOIUrl":"https://doi.org/10.1117/12.2057788","url":null,"abstract":"Care for head and neck (HN) cancer could be improved with better mapping of lymphatic drainage pathways in HN region as well as understanding the effect of the cancer treatments on lymphatics. In this study, near-infrared fluorescence imaging is being used to visualize the lymphatics in human subjects diagnosed with HN cancer before and after treatments. Imaging results show the lymphatic architecture and contractile function in HN. Reformation of lymphatics during the course of cancer care was also seen in the longitudinal imaging. This allows us to better understand the lymphatics in HN cancer patients.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"13 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85037938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Bone optical spectroscopy for the measurement of hemoglobin content 测定血红蛋白含量的骨光谱学
Translational biophotonics Pub Date : 2014-05-19 DOI: 10.1117/12.2060716
J. Hollmann, P. Arambel, J. Piet, S. Shefelbine, Stacey Markovic, M. Niedre, C. DiMarzio
{"title":"Bone optical spectroscopy for the measurement of hemoglobin content","authors":"J. Hollmann, P. Arambel, J. Piet, S. Shefelbine, Stacey Markovic, M. Niedre, C. DiMarzio","doi":"10.1117/12.2060716","DOIUrl":"https://doi.org/10.1117/12.2060716","url":null,"abstract":"Osteoporosis is a common side effect of spinal cord injuries. Blood perfusion in the bone provides an indication of bone health and may help to evaluate therapies addressing bone loss. Current methods for measuring blood perfusion of bone use dyes and ionizing radiation, and yield qualitative results. We present a device capable of measuring blood oxygenation in the tibia. The device illuminates the skin directly over the tibia with a white light source and measures the diffusely reflected light in the near infrared spectrum. Multiple source-detector distances are utilized so that the blood perfusion in skin and bone may be differentiated.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74067144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Development and optimization of a line-scanned dual-axis confocal (LS-DAC) microscope for high-speed pathology 线扫描双轴共聚焦(LS-DAC)高速病理显微镜的研制与优化
Translational biophotonics Pub Date : 2014-05-19 DOI: 10.1117/12.2057743
Danni Wang, Ye Chen, D. Meza, Y. Wang, Jonathan T. C. Liu
{"title":"Development and optimization of a line-scanned dual-axis confocal (LS-DAC) microscope for high-speed pathology","authors":"Danni Wang, Ye Chen, D. Meza, Y. Wang, Jonathan T. C. Liu","doi":"10.1117/12.2057743","DOIUrl":"https://doi.org/10.1117/12.2057743","url":null,"abstract":"We have developed a line-scanned dual-axis confocal (LS-DAC) microscope with subcellular resolution suitable for real time diagnostic imaging at shallow depths. This design serves as a benchtop prototype for a handheld version of the LS-DAC intended for rapid point-of-care pathology. We have assessed the performance trade-offs between the LS-DAC and a point-scanned dual-axis confocal (PS-DAC) microscope via diffraction-theory analysis, Monte-Carlo simulations, and characterization experiments with phantoms and fresh tissues. In addition, we are exploring the use of a sCMOS detector array and rapid 3D deconvolution to improve the sensitivity and resolution of our LS-DAC microscope.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"88734574","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Intradermal administration of fluorescent contrast agents for delivery to axillary lymph nodes 腋窝淋巴结皮下注射荧光造影剂
Translational biophotonics Pub Date : 2014-05-19 DOI: 10.1117/12.2057640
J. Rasmussen, Funda Meric-Berstam, S. Krishnamurthy, I. Tan, B. Zhu, J. Wagner, G. Babiera, E. Mittendorf, E. Sevick-Muraca
{"title":"Intradermal administration of fluorescent contrast agents for delivery to axillary lymph nodes","authors":"J. Rasmussen, Funda Meric-Berstam, S. Krishnamurthy, I. Tan, B. Zhu, J. Wagner, G. Babiera, E. Mittendorf, E. Sevick-Muraca","doi":"10.1117/12.2057640","DOIUrl":"https://doi.org/10.1117/12.2057640","url":null,"abstract":"In this proof-of-concept study we seek to demonstrate the delivery of fluorescent contrast agent to the tumor-draining lymph node basin following intraparenchymal breast injections and intradermal arm injection of micrograms of indocyanine green in 20 breast cancer patients undergoing complete axillary lymph node dissection. Individual lymph nodes were assessed ex vivo for presence of fluorescent signal. In all, 88% of tumor-negative lymph nodes and 81% of tumor-positive lymph nodes were fluorescent. These results indicate that future studies utilizing targeted fluorescent contrast agents may demonstrate improved surgical and therapeutic intervention.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83010931","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Automated frame selection process for analyzing high resolution microendoscope images 用于分析高分辨率显微内窥镜图像的自动帧选择过程
Translational biophotonics Pub Date : 2014-05-19 DOI: 10.1117/12.2062586
A. Ishijima, Sharon Mondrik, R. Schwarz, N. Vigneswaran, A. Gillenwater, R. Richards-Kortum
{"title":"Automated frame selection process for analyzing high resolution microendoscope images","authors":"A. Ishijima, Sharon Mondrik, R. Schwarz, N. Vigneswaran, A. Gillenwater, R. Richards-Kortum","doi":"10.1117/12.2062586","DOIUrl":"https://doi.org/10.1117/12.2062586","url":null,"abstract":"We developed an automated frame selection algorithm for high resolution microendoscope images. The algorithm rapidly selects a representative frame with minimal motion artifact from a short video sequence, enabling fully automated image analysis at the point-of-care. The performance of the algorithm was evaluated by comparing automatically selected frames to manually selected frames using quantitative image parameters. The implementation of fully automated high-resolution microendoscopy at the point-of-care has the potential to reduce the number of biopsies needed for accurate diagnosis of precancer and cancer in low-resource settings, where there may be limited infrastructure and personnel for standard histologic analysis.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89243806","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 1
Improvements in frequency-domain based NIRF optical tomography modality for preclinical studies 用于临床前研究的基于频域的NIRF光学断层扫描模式的改进
Translational biophotonics Pub Date : 2014-05-19 DOI: 10.1117/12.2057809
C. Darne, E. Sevick-Muraca
{"title":"Improvements in frequency-domain based NIRF optical tomography modality for preclinical studies","authors":"C. Darne, E. Sevick-Muraca","doi":"10.1117/12.2057809","DOIUrl":"https://doi.org/10.1117/12.2057809","url":null,"abstract":"Herein we present recent improvements in system design and performance evaluation of near-infrared fluorescence (NIRF) frequency-domain photon migration (FDPM) system developed for small animal fluorescence tomography and installed within a commercial micro-CT/PET scanner. We improved system performance by increasing signal-to-noise ratio (SNR) through use of high powered rf modulation, novel data collection scheme, and data discrimination based on the associated noise levels. Noise characteristics show improvement with these techniques and are currently being employed to improve 3-D fluorescence for tomographic reconstructions in phantoms before incorporating into hybrid scanner.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77511728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Wide spectral-range imaging spectroscopy of photonic crystal microbeads for multiplex biomolecular assay applications 光子晶体微珠的宽光谱范围成像光谱用于多种生物分子分析应用
Translational biophotonics Pub Date : 2014-05-19 DOI: 10.1117/12.2054711
Jianping Li
{"title":"Wide spectral-range imaging spectroscopy of photonic crystal microbeads for multiplex biomolecular assay applications","authors":"Jianping Li","doi":"10.1117/12.2054711","DOIUrl":"https://doi.org/10.1117/12.2054711","url":null,"abstract":"Suspension assay using optically color-encoded microbeads is a novel way to increase the reaction speed and multiplex of biomolecular detection and analysis. To boost the detection speed, a hyperspectral imaging (HSI) system is of great interest for quickly decoding the color codes of the microcarriers. Imaging Fourier transform spectrometer (IFTS) is a potential candidate for this task due to its advantages in HSI measurement. However, conventional IFTS is only popular in IR spectral bands because it is easier to track its scanning mirror position in longer wavelengths so that the fundamental Nyquist criterion can be satisfied when sampling the interferograms; the sampling mechanism for shorter wavelengths IFTS used to be very sophisticated, high-cost and bulky. In order to overcome this handicap and take better usage of its advantages for HSI applications, a new wide spectral range IFTS platform is proposed based on an optical beam-folding position-tracking technique. This simple technique has successfully extended the spectral range of an IFTS to cover 350-1000nm. Test results prove that the system has achieved good spectral and spatial resolving performances with instrumentation flexibilities. Accurate and fast measurement results on novel colloidal photonic crystal microbeads also demonstrate its practical potential for high-throughput and multiplex suspension molecular assays.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"33 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78233216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Performance evaluation of integrating detectors for near-infrared fluorescence molecular imaging 近红外荧光分子成像积分探测器的性能评价
Translational biophotonics Pub Date : 2014-05-19 DOI: 10.1117/12.2057815
B. Zhu, J. Rasmussen, E. Sevick-Muraca
{"title":"Performance evaluation of integrating detectors for near-infrared fluorescence molecular imaging","authors":"B. Zhu, J. Rasmussen, E. Sevick-Muraca","doi":"10.1117/12.2057815","DOIUrl":"https://doi.org/10.1117/12.2057815","url":null,"abstract":"Although there has been a plethora of devices advanced for clinical translation, there has been no standards to compare and determine the optical device for fluorescence molecular imaging. In this work, we compare different CCD configurations using a solid phantom developed to mimic pM - fM concentrations of near-infrared fluorescent dyes in tissues. Our results show that intensified CCD systems (ICCDs) offer greater contrast at larger signal-tonoise ratios (SNRs) in comparison to their un-intensified CCD systems operated at clinically reasonable, sub-second acquisition times. Furthermore, we compared our investigational ICCD device to the commercial NOVADAQ SPY system, demonstrating different performance in both SNR and contrast.","PeriodicalId":75242,"journal":{"name":"Translational biophotonics","volume":"25 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2014-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78935189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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