American journal of clinical pathology最新文献_第4页

Correction to: The American Society for Clinical Pathology's 2023 wage survey of medical laboratories in the United States. 更正：美国临床病理学会 2023 年美国医学实验室工资调查。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-11-08 DOI: 10.1093/ajcp/aqae152

引用次数: 0

Integrative immunophenotypic and genetic characterization of acute myeloid leukemia with CBFB rearrangement. 带有 CBFB 重排的急性髓性白血病的免疫表型和遗传特征综合分析。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-11-04 DOI: 10.1093/ajcp/aqae060

Fnu Sameeta, Sa A Wang, Zhenya Tang, Joseph D Khoury, Hong Fang, Dylan Wang, Jie Xu, Shaoying Li, Zhihong Hu, Shimin Hu, Jeffrey L Jorgensen, L Jeffrey Medeiros, Wei Wang

{"title":"Integrative immunophenotypic and genetic characterization of acute myeloid leukemia with CBFB rearrangement.","authors":"Fnu Sameeta, Sa A Wang, Zhenya Tang, Joseph D Khoury, Hong Fang, Dylan Wang, Jie Xu, Shaoying Li, Zhihong Hu, Shimin Hu, Jeffrey L Jorgensen, L Jeffrey Medeiros, Wei Wang","doi":"10.1093/ajcp/aqae060","DOIUrl":"10.1093/ajcp/aqae060","url":null,"abstract":"Objectives: We sought to characterize the immunophenotype of acute myeloid leukemia (AML) with CBFB rearrangement and correlate the results with cytogenetic and molecular data.Methods: Sixty-one cases of AML with CBFB rearrangement were evaluated.Results: The sample population consisted of 33 men and 28 women, with a median age of 49 years. Flow cytometry immunophenotypic analysis showed that myeloblasts were positive for CD34 and CD117 in all cases, and myeloperoxidase was positive in 52 of 55 (95%) cases. The most common abnormalities included decreased CD38 in 90%, increased CD13 in 85%, increased CD123 in 84%, and decreased HLA-DR in 84% of cases. Monocytes were increased, with a mature immunophenotype, and accounted for 23.7% of total cells. Among 60 cases with available karyotype, inv(16)(p13.1q22) was most common in 50 (83%) cases, followed by t(16;16) (p13.1;q22) in 6 (10%). Type A CBFB::MYH11 transcript was most common, detected in 84% of cases. Mutational analysis showed mutations of NRAS in 37%, FLT3 in 25%, and KIT in 24% of cases. Comparing cases with type A vs non-type A transcripts, blasts in type A cases more frequently exhibited CD64 positivity and increased CD13 levels while showing a lower frequency of CD7 and CD56 expression. Trisomy 22 and mutations in KIT, NF1, and TET2 were identified only in cases with type A transcript.Conclusions: Myeloblasts of AML with CBFB rearrangement are positive for CD34, CD117, and myeloperoxidase. These neoplasms most frequently carry inv(16)(p13.1q22) and type A fusion transcript. NRAS mutation was the most common mutation. Some immunophenotypic and genetic correlations occurred with different types of transcripts.","PeriodicalId":7506,"journal":{"name":"American journal of clinical pathology","volume":" ","pages":"455-463"},"PeriodicalIF":2.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141154551","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic accuracy of upper tract urothelial carcinoma using biopsy, urinary cytology, and nephroureterectomy specimens: A tertiary cancer center experience. 利用活组织检查、尿液细胞学检查和肾切除术标本诊断上尿路上皮癌的准确性：一家三级癌症中心的经验。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-11-04 DOI: 10.1093/ajcp/aqae065

Jianping Zhao, Yuan Shen, Ming Guo, Surena F Matin, Donna E Hansel, Charles C Guo

{"title":"Diagnostic accuracy of upper tract urothelial carcinoma using biopsy, urinary cytology, and nephroureterectomy specimens: A tertiary cancer center experience.","authors":"Jianping Zhao, Yuan Shen, Ming Guo, Surena F Matin, Donna E Hansel, Charles C Guo","doi":"10.1093/ajcp/aqae065","DOIUrl":"10.1093/ajcp/aqae065","url":null,"abstract":"Objectives: We studied the diagnostic accuracy and discordance of upper tract urothelial carcinoma (UTUC) by comparing biopsy and urinary cytology with matched nephroureterectomy specimens.Methods: Sixty-nine patients with UTUC without neoadjuvant treatment were retrospectively identified who had matched biopsy and nephroureterectomy specimens. Twenty patients had concurrent upper tract cytology. H&E and cytology slides were re-reviewed. Statistical analysis was performed.Results: Patients included 48 men and 21 women with a mean age of 69 years. A concordant grade between biopsy and surgical specimen was present in 49 (71%) patients. The mean size of biopsy specimens in the discordant group was significantly smaller than that in the concordant group. Invasion was evaluated in 48 biopsy cases that had adequate subepithelial tissue, and 33 of them were diagnosed with concordant invasion status. Mean tumor size in both tumor grade and invasion discordant groups was significantly larger than that in the concordant group. High-grade urothelial carcinoma was detected in 84% of cases using urinary cytology.Conclusions: Our study demonstrates the diagnostic challenges of UTUC on small biopsy specimens. Biopsy specimen size and tumor size are significantly associated with the diagnostic discordance. Upper tract cytology showed high diagnostic accuracy and should be complementary to the biopsy.","PeriodicalId":7506,"journal":{"name":"American journal of clinical pathology","volume":" ","pages":"492-499"},"PeriodicalIF":2.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141299707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reply to "Performance of GPT-4 Vision on kidney pathology exam questions". 答复 "GPT-4 Vision 在肾脏病理学试题中的表现"。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-11-04 DOI: 10.1093/ajcp/aqae059

Jing Miao, Charat Thongprayoon, Wisit Cheungpasitporn, Lynn D Cornell

引用次数: 0

Mobile calculator application for estimating human erythrocyte antigen frequency in Korea. 用于估算韩国人类红细胞抗原频率的移动计算器应用程序。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-11-04 DOI: 10.1093/ajcp/aqae070

Dong Woo Shin, Yun Ji Hong, Kyoung Un Park

{"title":"Mobile calculator application for estimating human erythrocyte antigen frequency in Korea.","authors":"Dong Woo Shin, Yun Ji Hong, Kyoung Un Park","doi":"10.1093/ajcp/aqae070","DOIUrl":"10.1093/ajcp/aqae070","url":null,"abstract":"Objectives: This study aimed to establish a comprehensive human erythrocyte antigen (HEA) frequency data set for Koreans. It also sought to develop a mobile app that facilitates the calculation of the frequencies of specific antigen-negative red blood cell units and the average number of units required for antigen typing.Methods: Human erythrocyte antigen frequencies were compiled from large-scale blood donor data and 5 previous papers. Based on the collected data, we developed a mobile calculator app for HEA frequency and evaluated its usability.Results: Human erythrocyte antigen frequency data for 20 blood group systems, including the ABO, Rh, MNS, Duffy, Kidd, and Diego systems, were established. The app was designed to enable users to select the desired phenotype from a drop-down menu and display the calculated frequency at the bottom. The number of units required for antigen typing to find 1 compatible red blood cell unit was also displayed. Five users participated in app evaluation and rated the functionality and information categories highly. In quizzes prompting users to calculate frequencies using the app, all participants provided correct answers, confirming the app's user-friendly functionality.Conclusions: This app, which encompasses comprehensive HEA frequency data, is expected to find multiple uses in transfusion medicine, including optimizing blood bank workflow and defining rare blood groups in Korea.","PeriodicalId":7506,"journal":{"name":"American journal of clinical pathology","volume":" ","pages":"521-528"},"PeriodicalIF":2.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141309421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Variations in emergency hemorrhage panel turnaround times in 2 major medical centers using the same laboratory methods. 两家大型医疗中心使用相同实验室方法进行急诊出血检查的周转时间差异。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-11-04 DOI: 10.1093/ajcp/aqae071

Matthew E Hogan, Zhinan Liu, Lynn G Stansbury, Monica S Vavilala, John R Hess, Hamilton C Tsang

{"title":"Variations in emergency hemorrhage panel turnaround times in 2 major medical centers using the same laboratory methods.","authors":"Matthew E Hogan, Zhinan Liu, Lynn G Stansbury, Monica S Vavilala, John R Hess, Hamilton C Tsang","doi":"10.1093/ajcp/aqae071","DOIUrl":"10.1093/ajcp/aqae071","url":null,"abstract":"Objectives: Demand for rapid coagulation testing for massive transfusion events led to development of an emergency hemorrhage panel (EHP; hemoglobin, platelet count, prothrombin time/international normalized ratio, and fibrinogen), with laboratory turnaround time (TAT) of less than 20 minutes. Ten years on, we asked if current laboratory practices were meeting that TAT goal and differences were evident in TAT between the 2 major institutions in our system.Methods: We identified EHPs ordered at our 2 largest hospitals, February 2, 2021, to July 17, 2022, comparing order to specimen draw time, specimen draw to specimen received time, laboratory analytic time, and total TAT results from emergency department and operating room. Site 1 houses a level I trauma center; site 2 includes tertiary care, transplant, and obstetrics services.Results: In total, 1137 EHPs were recorded in our study period. Laboratory TAT was significantly faster at site 1 (~14 vs ~27 minutes, P < .01). Average laboratory TAT was under 20 minutes at site 1 but only for 50% of specimens at site 2. Outlier specimens were collection delays at site 1 and specimen processing delays at site 2.Conclusions: The EHP can be performed as rapidly as described. However, compromises in laboratory location, available personnel, and processing differences can degrade performance.","PeriodicalId":7506,"journal":{"name":"American journal of clinical pathology","volume":" ","pages":"529-534"},"PeriodicalIF":2.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141330136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reply to "Predictors of clinical outcome in myeloproliferative neoplasm, unclassifiable: a Bone Marrow Pathology Group study". 对 "骨髓增生性肿瘤（不可分类）临床预后的预测因素：骨髓病理学小组的一项研究 "的答复

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-11-04 DOI: 10.1093/ajcp/aqae142

Genevieve M Crane, Yan Xie, Heesun J Rogers

引用次数: 0

High α-SMA expression in the tumor stroma is associated with adverse clinical parameters in mismatch repair-proficient colorectal cancers only. 肿瘤基质中的α-SMA高表达仅与错配修复功能良好的结直肠癌的不良临床指标有关。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-11-04 DOI: 10.1093/ajcp/aqae145

Declan J Sculthorpe, Amy Denton, Wakkas Fadhil, Dewi Rusnita, Mohammad Ilyas, Abhik Mukherjee

{"title":"High α-SMA expression in the tumor stroma is associated with adverse clinical parameters in mismatch repair-proficient colorectal cancers only.","authors":"Declan J Sculthorpe, Amy Denton, Wakkas Fadhil, Dewi Rusnita, Mohammad Ilyas, Abhik Mukherjee","doi":"10.1093/ajcp/aqae145","DOIUrl":"https://doi.org/10.1093/ajcp/aqae145","url":null,"abstract":"Objectives: As mismatch repair status confers differential prognosis in colorectal cancers, this study aimed to determine associations of α-smooth muscle actin (α-SMA) protein expression in mismatch repair-proficient (pMMR) and mismatch repair-deficient (dMMR) colorectal tumors with clinicopathologic and prognostic features.Methods: Tissue microarrays from patients with colorectal cancer, immunostained with α-SMA, were assessed through digital image analysis. Total (n = 962), pMMR (n = 782), and dMMR (n = 156) stromal H-scores were assessed for associations with clinicopathologic and survival data.Results: Higher α-SMA expression was correlated with pMMR status (P = 5.2223 × 10-8). In the pMMR subgroup, higher α-SMA stromal expression at the tumor periphery was correlated with higher T stage (P = .002), perineural invasion (P = .038), infiltrative tumor edge (P = .01), involved nodal status (P = .036), metastases (P = .013), synchronous metastases (P = .007), recurrence (P = .004), and both 3-year and 5-year survival (P = .018). dMMR tumors showed no significant correlations with α-SMA staining.Conclusions: The findings highlight that immunostaining with α-SMA in pMMR colorectal tumors, especially at the tumor periphery, has the potential to identify patients with adverse prognostic features. Digital assessment of α-SMA may offer improved objectivity, accuracy, economy of time, and risk stratification for management.","PeriodicalId":7506,"journal":{"name":"American journal of clinical pathology","volume":" ","pages":""},"PeriodicalIF":2.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142567051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinical validation of the Ion Torrent Oncomine Myeloid Assay GX v2 on the Genexus Integrated Sequencer as a stand-alone assay for single-nucleotide variants, insertions/deletions, and fusion genes: Challenges, performance, and perspectives. Ion Torrent Oncomine骨髓测定GX v2在Genexus集成测序仪上作为单核苷酸变异、插入/缺失和融合基因的独立测定的临床验证：挑战、性能和前景。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-11-04 DOI: 10.1093/ajcp/aqae063

Kritika Krishnamurthy, Jiani Chai, Xiaowei Liu, Yanhua Wang, Rizwan Naeem, D Yitzchak Goldstein

{"title":"Clinical validation of the Ion Torrent Oncomine Myeloid Assay GX v2 on the Genexus Integrated Sequencer as a stand-alone assay for single-nucleotide variants, insertions/deletions, and fusion genes: Challenges, performance, and perspectives.","authors":"Kritika Krishnamurthy, Jiani Chai, Xiaowei Liu, Yanhua Wang, Rizwan Naeem, D Yitzchak Goldstein","doi":"10.1093/ajcp/aqae063","DOIUrl":"10.1093/ajcp/aqae063","url":null,"abstract":"Objectives: Myeloid neoplasms require comprehensive characterization of genetic abnormalities, including single-nucleotide variants, small insertions and deletions, and fusions and translocations for management. The Oncomine Myeloid Assay GX v2 (Thermo Fisher Scientific) analyzes 17 full genes, 28 hotspot genes, 30 fusion driver genes, and 5 expression genes.Methods: The validation set included 192 DNA samples, 28 RNA samples, and 9 cell lines and contrived controls. The DNA and RNA were extracted from both peripheral blood and bone marrow. Library preparation, templating, and sequencing was performed on the fully automated Genexus Integrated Sequencer (Thermo Fisher Scientific). The sequencing data were analyzed by manual curation, default Oncomine filters and the Oncomine Reporter (Thermo Fisher Scientific).Results: Of the 600 reference pathogenic DNA variants targeted by the assay, concordance was seen in 98.3% of unfiltered variant call format files. Precision and reproducibility were 100%, and the lower limit of detection was 2% variant allele frequency for DNA. Inability to detect variants in long homopolymer regions intrinsic to the Ion Torrent chemistry led to 7 missed variants; 100% concordance was seen with reference RNA samples.Conclusions: This extensive clinical validation of the Oncomine Myeloid Assay GX v2 on the Genexus Integrated Sequencer with its built-in bioinformatics pipeline and Ion Torrent Oncomine Reporter shows robust performance in terms of variant calling accuracy, precision, and reproducibility, with the advantage of a rapid turnaround time of 2 days. The greatest limitation is the inability to detect variants in long homopolymer regions.","PeriodicalId":7506,"journal":{"name":"American journal of clinical pathology","volume":" ","pages":"480-491"},"PeriodicalIF":2.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141186309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tennessee hospital noncompliance with price transparency legislation for 8 common laboratory tests. 田纳西州医院在 8 项常见化验项目上不遵守价格透明法规的情况。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-11-04 DOI: 10.1093/ajcp/aqae057

Stephanie A Hart, Ayesha Khan, Garrett S Booth, Joesph R Wiencek

{"title":"Tennessee hospital noncompliance with price transparency legislation for 8 common laboratory tests.","authors":"Stephanie A Hart, Ayesha Khan, Garrett S Booth, Joesph R Wiencek","doi":"10.1093/ajcp/aqae057","DOIUrl":"10.1093/ajcp/aqae057","url":null,"abstract":"Objectives: The goal of this study was to assess hospital compliance with federal price transparency mandates and barriers to pricing information in Tennessee.Methods: All hospitals websites were queried for gross, cash, and BlueCross BlueShield of Tennessee prices for 8 high-frequency laboratory tests in 2 Centers for Medicare & Medicaid Services-mandated pricing sources: (1) a machine-readable file of all available services and (2) a consumer-friendly display of 300 shoppable services. Barriers, including click counts, data availability, and intrahospital price discrepancies, were noted.Results: Of the 145 Tennessee hospitals assessed, 97.2% were noncompliant with the Centers for Medicare & Medicaid Services final rule. Subanalysis of available machine-readable files, price estimators, and shoppable services files demonstrated 49.6%, 95.1%, and 78.6% noncompliance, respectively. Barriers to pricing information included requiring protected health information (55.9%), missing at least 1 pricing source (7.6%), having no pricing sources available (6.2%), and involving more than 3 clicks to access the cash price in machine-readable files (54.1%) and price estimators (68.6%.) Average intrahospital discrepancy for basic metabolic panel cash prices across pricing sources was $101.30 (range, $0-1012.40).Conclusions: Our study showed high levels of noncompliance with price transparency laws, inconsistent and inaccessible pricing, and continued challenges facing patients in Tennessee.","PeriodicalId":7506,"journal":{"name":"American journal of clinical pathology","volume":" ","pages":"450-454"},"PeriodicalIF":2.3,"publicationDate":"2024-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11532617/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140908238","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0