American journal of clinical pathology最新文献_第4页

The American Society for Clinical Pathology's 2023 wage survey of medical laboratories in the United States. 美国临床病理学会 2023 年美国医学实验室工资调查。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-10-07 DOI: 10.1093/ajcp/aqae130

Edna Garcia, Iman Kundu, Karen Fong

{"title":"The American Society for Clinical Pathology's 2023 wage survey of medical laboratories in the United States.","authors":"Edna Garcia, Iman Kundu, Karen Fong","doi":"10.1093/ajcp/aqae130","DOIUrl":"10.1093/ajcp/aqae130","url":null,"abstract":"Objectives: To inform the pathology and laboratory field of the most recent national wage data. Historically, the results of this biennial survey have served as a basis for additional research on laboratory professional recruitment, retention, education, marketing, certification, and advocacy.Methods: The 2023 Wage Survey was conducted in collaboration between the American Society for Clinical Pathology's (ASCP's) Institute of Science, Technology, and Policy in Washington, DC, and ASCP Board of Certification in Chicago, Illinois.Results: Compared to 2021, results show that more occupations at the staff level had an increased average hourly wage (pathologists' assistant, molecular biology technologist, phlebotomist, cytogenetic technologist, and medical laboratory technician [MLT]), after adjusting for inflation. Wages by time in current occupational title are significantly higher in 2023 than in 2021. Histotechnicians, histotechnologists, and MLTs show consistent increases in pay rates for a longer length of time in the laboratory. Over half (52.4%) of the respondents feel the pandemic continues to influence their salary and/or well-being.Conclusions: Survey results call for continued efforts in promoting visibility of the profession and greater representation through advocacy. While burnout rates are lower compared to 2021, staffing challenges remain a relevant concern. Efforts to support the workforce have multiplied since the pandemic and have been the forefront focus of the laboratory community. However, continued support and advocacy are needed to increase the promotion and value of laboratory careers for laboratory professionals and patients alike.","PeriodicalId":7506,"journal":{"name":"American journal of clinical pathology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histomorphologic spectrum of nodal marginal zone lymphoma as defined by its methylome. 根据甲基组定义的结节边缘区淋巴瘤组织形态学谱系。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-10-07 DOI: 10.1093/ajcp/aqae120

Francesca Spada, Andreas Rosenwald, Wolfram Klapper, Alfred C Feller, Falko Fend, German Ott, Axel Fürstberger, Thomas F E Barth, Peter Möller

{"title":"Histomorphologic spectrum of nodal marginal zone lymphoma as defined by its methylome.","authors":"Francesca Spada, Andreas Rosenwald, Wolfram Klapper, Alfred C Feller, Falko Fend, German Ott, Axel Fürstberger, Thomas F E Barth, Peter Möller","doi":"10.1093/ajcp/aqae120","DOIUrl":"https://doi.org/10.1093/ajcp/aqae120","url":null,"abstract":"Objectives: Primary nodal marginal B-cell lymphoma (NMZL) is rare and histologically very variable. Its large-cell presentation is difficult to distinguish from nodal diffuse large B-cell lymphoma (nDLBCL) due to the absence of specific markers for nodal marginal zone lymphomas in general.Methods: Using a comprehensive cohort of NMZLs and a control cohort of nDLBCLs, we conducted a methylome analysis on subgroups of both.Results: The methylomes were strikingly different between the cohorts but unexpectedly homogeneous within the NMZL cohort. This allowed us to describe the morphologic spectrum of NMZL in all its value ranges. The considerable overlap in growth pattern and cytology of NMZL with nDLBCL was explored morphometrically, leading to an operational tool for separating both by a simple measurement of cell size and nuclear size. This was integrated in a hierarchical approach, including a scoring system for the parameter growth pattern, follicular colonization, follicular dendritic network, IgD expression, and Ki-67 rate, and led to a proposal for a classifier that we present here.Conclusions: This methylome-based study extends the morphological spectrum of NMZL towards large cell morphology and offers a conventional way to distinguish it from nDLBCL.","PeriodicalId":7506,"journal":{"name":"American journal of clinical pathology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142379884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characteristics and utility of high-resolution optical coherence microscopy images of endocervical canal lesions. 宫颈管内膜病变的高分辨率光学相干显微镜图像的特点和实用性。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-10-03 DOI: 10.1093/ajcp/aqae052

Wei Zhang, Jeffrey L Fine, Xiaoyin Pei, Yushan Cao, Yixuan Liu, Jinxiang Yan, Zhenying Ban, Ting Zhang, Yuan Wei, Xin Zhao, Baojin Wang, Chengquan Zhao, Xianxu Zeng

{"title":"Characteristics and utility of high-resolution optical coherence microscopy images of endocervical canal lesions.","authors":"Wei Zhang, Jeffrey L Fine, Xiaoyin Pei, Yushan Cao, Yixuan Liu, Jinxiang Yan, Zhenying Ban, Ting Zhang, Yuan Wei, Xin Zhao, Baojin Wang, Chengquan Zhao, Xianxu Zeng","doi":"10.1093/ajcp/aqae052","DOIUrl":"10.1093/ajcp/aqae052","url":null,"abstract":"Objectives: To investigate optical coherence microscopy (OCM) imaging features and the application value of these high-resolution images for identifying endocervical canal lesions (ECLs), which is a clinical dilemma in cervical cancer screening programs.Methods: In total, 520 OCM images were obtained by scanning the cervical canal lesions with an ultra-high-resolution OCM system (204 specimens from 73 patients). The OCM morphologic characteristics of ECLs were observed and summarized, and then 3 researchers performed a diagnostic test of OCM images of cervical canal lesions. The accuracy, sensitivity, specificity, positive predictive value, negative predictive value, 95% confidence interval of each parameter, and interinvestigator agreement (κ) were calculated.Results: Normal endocervix, cysts, squamous metaplasia, high-grade squamous intraepithelial lesions involving glands, and invasive carcinoma had distinct OCM characteristics, which correlated well with corresponding H&E histologic sections. The accuracy, sensitivity, and specificity of the 3 researchers were 90.6%, 89.3% (95% CI, 86.5%-91.7%) and 91.6% (95% CI, 89.2%-93.5%), respectively. The positive predictive value was 90.1% (95% CI, 87.3%-92.4%), and the negative predictive value was 90.9% (95% CI, 88.5%-92.9%), with almost perfect agreement (κ = 0.874).Conclusions: The application of the OCM system in cervical canal lesions is feasible and could help improve detection of occult ECLs in cervical cancer screening programs. This study lays the foundation for further research on OCM in cervical canal lesions in vivo, which also has a potential impact on projecting pathologic evaluation beyond what is currently possible, perhaps globally.","PeriodicalId":7506,"journal":{"name":"American journal of clinical pathology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140855310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Establishing hemolysis, icterus, and lipemia interference limits for body fluid chemistry analytes measured on the Roche cobas instrument. 为罗氏 cobas 仪器测量的体液化学分析物建立溶血、黄疸和脂血干扰限。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-10-03 DOI: 10.1093/ajcp/aqae040

Darci R Block, Michael A Lasho, Leslie J Donato, Jeffrey W Meeusen

{"title":"Establishing hemolysis, icterus, and lipemia interference limits for body fluid chemistry analytes measured on the Roche cobas instrument.","authors":"Darci R Block, Michael A Lasho, Leslie J Donato, Jeffrey W Meeusen","doi":"10.1093/ajcp/aqae040","DOIUrl":"10.1093/ajcp/aqae040","url":null,"abstract":"Objectives: The aims of this study were to (1) establish the maximum allowable interference limits for hemolysis, lipemia, and icterus for chemistry analytes tested in body fluid samples and (2) assess the effectiveness of serial dilution to mitigate spectral interferences.Methods: Residual body fluids from clinically ordered testing were mixed (<10% by volume) with stock solutions of interferent (spiked) and compared with a control spiked with an equal volume of 0.9% saline. The analytes were measured on the Roche cobas c501 instrument. Difference and percentage difference were calculated and compared with allowable total error limits. A subset of samples were serially diluted with 0.9% saline. Mean (SD) difference and percentage difference were calculated.Results: The interference thresholds were lower than the package insert for lactate dehydrogenase, cholesterol, triglycerides, and total protein for hemolysis; amylase, cholesterol, and total protein for icterus; and albumin for lipemia. Only cholesterol and triglyceride results returned to baseline upon dilution of icteric samples.Conclusions: Interference thresholds in body fluids were lower than blood for 6 analytes. Diluting interferences that surpass these limits does not produce reliable results that are comparable to the baseline results before spiking in the interferent.","PeriodicalId":7506,"journal":{"name":"American journal of clinical pathology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140846750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dilute Russell viper venom time interpretation: Influence of lot changes and normalization. 稀释拉塞尔蝰蛇毒时间解读：批次变化和正常化的影响。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-10-03 DOI: 10.1093/ajcp/aqae054

Geoffrey D Wool

引用次数: 0

Harmonizing tumor mutational burden analysis: Insights from a multicenter study using in silico reference data sets in clinical whole-exome sequencing (WES). 统一肿瘤突变负荷分析：在临床全外显子测序（WES）中使用硅学参考数据集的多中心研究的启示。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-10-03 DOI: 10.1093/ajcp/aqae056

Lijia Yu, Yuanfeng Zhang, Duo Wang, Lin Li, Rui Zhang, Jinming Li

{"title":"Harmonizing tumor mutational burden analysis: Insights from a multicenter study using in silico reference data sets in clinical whole-exome sequencing (WES).","authors":"Lijia Yu, Yuanfeng Zhang, Duo Wang, Lin Li, Rui Zhang, Jinming Li","doi":"10.1093/ajcp/aqae056","DOIUrl":"10.1093/ajcp/aqae056","url":null,"abstract":"Objectives: Tumor mutational burden (TMB) is a significant biomarker for predicting immune checkpoint inhibitor response, but the clinical performance of whole-exome sequencing (WES)-based TMB estimation has received less attention compared to panel-based methods. This study aimed to assess the reliability and comparability of WES-based TMB analysis among laboratories under routine testing conditions.Methods: A multicenter study was conducted involving 24 laboratories in China using in silico reference data sets. The accuracy and comparability of TMB estimation were evaluated using matched tumor-normal data sets. Factors such as accuracy of variant calls, limit of detection (LOD) of WES test, size of regions of interest (ROIs) used for TMB calculation, and TMB cutoff points were analyzed.Results: The laboratories consistently underestimated the expected TMB scores in matched tumor-normal samples, with only 50% falling within the ±30% TMB interval. Samples with low TMB score (<2.5) received the consensus interpretation. Accuracy of variant calls, LOD of the WES test, ROI, and TMB cutoff points were important factors causing interlaboratory deviations.Conclusions: This study highlights real-world challenges in WES-based TMB analysis that need to be improved and optimized. This research will aid in the selection of more reasonable analytical procedures to minimize potential methodologic biases in estimating TMB in clinical exome sequencing tests. Harmonizing TMB estimation in clinical testing conditions is crucial for accurately evaluating patients' response to immunotherapy.","PeriodicalId":7506,"journal":{"name":"American journal of clinical pathology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140908232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Artificial intelligence-aided steatosis assessment in donor livers according to the Banff consensus recommendations. 根据班夫共识建议对供体肝脏进行人工智能辅助脂肪变性评估。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-10-03 DOI: 10.1093/ajcp/aqae053

Jingjing Jiao, Haiming Tang, Nanfei Sun, Xuchen Zhang

{"title":"Artificial intelligence-aided steatosis assessment in donor livers according to the Banff consensus recommendations.","authors":"Jingjing Jiao, Haiming Tang, Nanfei Sun, Xuchen Zhang","doi":"10.1093/ajcp/aqae053","DOIUrl":"10.1093/ajcp/aqae053","url":null,"abstract":"Objectives: Severe macrovesicular steatosis in donor livers is associated with primary graft dysfunction. The Banff Working Group on Liver Allograft Pathology has proposed recommendations for steatosis assessment of donor liver biopsy specimens with a consensus for defining \"large droplet fat\" (LDF) and a 3-step algorithmic approach.Methods: We retrieved slides and initial pathology reports from potential liver donor biopsy specimens from 2010 to 2021. Following the Banff approach, we reevaluated LDF steatosis and employed a computer-assisted manual quantification protocol and artificial intelligence (AI) model for analysis.Results: In a total of 113 slides from 88 donors, no to mild (<33%) macrovesicular steatosis was reported in 88.5% (100/113) of slides; 8.8% (10/113) was reported as at least moderate steatosis (≥33%) initially. Subsequent pathology evaluation, following the Banff recommendation, revealed that all slides had LDF below 33%, a finding confirmed through computer-assisted manual quantification and an AI model. Correlation coefficients between pathologist and computer-assisted manual quantification, between computer-assisted manual quantification and the AI model, and between the AI model and pathologist were 0.94, 0.88, and 0.81, respectively (P < .0001 for all).Conclusions: The 3-step approach proposed by the Banff Working Group on Liver Allograft Pathology may be followed when evaluating steatosis in donor livers. The AI model can provide a rapid and objective assessment of liver steatosis.","PeriodicalId":7506,"journal":{"name":"American journal of clinical pathology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140875578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quality improvements are effective in reducing preanalytical errors and rejection rates in clinical laboratories. 质量改进可有效降低临床实验室的分析前误差和废品率。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-10-03 DOI: 10.1093/ajcp/aqae050

Mohammed Suleiman, Andrés Pérez-López, Patrick Tang

引用次数: 0

A simple risk stratification model for prostate cancer using histopathologic findings of radical prostatectomy. 利用根治性前列腺切除术的组织病理学结果建立简单的前列腺癌风险分层模型。

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-10-03 DOI: 10.1093/ajcp/aqae049

Remi Semba, Katsunori Uchida, Yoshihumi Hirokawa, Taizo Shiraishi, Takehisa Onishi, Takeshi Sasaki, Takahiro Inoue, Masatoshi Watanabe, Hiroshi Miyamoto

{"title":"A simple risk stratification model for prostate cancer using histopathologic findings of radical prostatectomy.","authors":"Remi Semba, Katsunori Uchida, Yoshihumi Hirokawa, Taizo Shiraishi, Takehisa Onishi, Takeshi Sasaki, Takahiro Inoue, Masatoshi Watanabe, Hiroshi Miyamoto","doi":"10.1093/ajcp/aqae049","DOIUrl":"10.1093/ajcp/aqae049","url":null,"abstract":"Objectives: To develop a simple postoperative risk stratification based on histopathologic findings from radical prostatectomy specimens.Methods: This study included 3 cohorts of patients with a preoperative diagnosis of clinically localized prostate cancer: 1 derivation cohort (n = 432) and 2 validation cohorts (n = 506 and n = 720). First, a postoperative risk stratification model was developed in the derivation cohort using the factors extraprostatic extension, surgical margin status, seminal vesicle invasion, and lymph node involvement. Each of the first 3 factors was assigned 0 or 1 point for negative or positive results, respectively, and the sum of the points, ranging from 0 to 3, was scored. pN1 was not scored but was analyzed separately. Validation cohorts were then used to evaluate the predictive accuracy of the model. Additionally, we compared the model with the Cancer of the Prostate Risk Assessment (CAPRA) score.Results: Because the log-rank test showed no statistically significant differences between scores 1 vs 2 or score 3 vs pN1 in the derivation cohort, the following 3-level risk stratification was created: low risk (score 0), intermediate risk (score 1-2), and high risk (score 3 or pN1). There were statistically significant differences in recurrence-free survival between any of 2 groups of 3-level risk stratification. This model similarly worked in both validation cohorts. The C indexes for the model were higher than those for the CAPRA score.Conclusions: This simple postoperative risk stratification model, based on radical prostatectomy findings, has a prognostic impact that has been validated in a multicenter population.","PeriodicalId":7506,"journal":{"name":"American journal of clinical pathology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140848307","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

How I diagnose systemic mastocytosis. 如何诊断全身性肥大细胞增多症？

IF 2.3 4区医学

American journal of clinical pathology Pub Date : 2024-10-03 DOI: 10.1093/ajcp/aqae047

Anton V Rets, Tracy I George

{"title":"How I diagnose systemic mastocytosis.","authors":"Anton V Rets, Tracy I George","doi":"10.1093/ajcp/aqae047","DOIUrl":"10.1093/ajcp/aqae047","url":null,"abstract":"Objectives: Systemic mastocytosis (SM) is a neoplasm of mast cells (MCs) characterized by their proliferation in extracutaneous organs. Systemic mastocytosis includes several entities with different clinical courses and prognoses. The rarity of this disease and the diversity of clinical and morphologic presentation make the diagnosis of SM very challenging. The aim of this review is to share our approach to the diagnosis of SM.Methods: We present 4 cases that highlight the spectrum of clinical and laboratory features of SM and outline the diagnostic process with an emphasis on morphology.Results: Pathology and laboratory medicine play a key role in investigation of SM, as correct diagnosis requires integration of morphologic, molecular, and serologic findings. In addition to awareness of microscopic findings in SM, a pathologist must keep abreast with an expanding menu of ancillary studies, particularly molecular testing.Conclusions: Systemic mastocytosis is a challenging diagnosis that requires not only a demonstration of a clonal proliferation of MCs but also a correct subclassification based on the recently updated criteria.","PeriodicalId":7506,"journal":{"name":"American journal of clinical pathology","volume":null,"pages":null},"PeriodicalIF":2.3,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140855311","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0