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Molecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maize. ZmRL6正调控玉米抗旱性的分子机制分析。
Stress biology Pub Date : 2023-11-16 DOI: 10.1007/s44154-023-00125-x
Pengyu Zhang, Tongchao Wang, Liru Cao, Zhixin Jiao, Lixia Ku, Dandan Dou, Zhixue Liu, Jiaxu Fu, Xiaowen Xie, Yingfang Zhu, Leelyn Chong, Li Wei
{"title":"Molecular mechanism analysis of ZmRL6 positively regulating drought stress tolerance in maize.","authors":"Pengyu Zhang, Tongchao Wang, Liru Cao, Zhixin Jiao, Lixia Ku, Dandan Dou, Zhixue Liu, Jiaxu Fu, Xiaowen Xie, Yingfang Zhu, Leelyn Chong, Li Wei","doi":"10.1007/s44154-023-00125-x","DOIUrl":"10.1007/s44154-023-00125-x","url":null,"abstract":"<p><p>MYB-related genes, a subclass of MYB transcription factor family, have been documented to play important roles in biological processes such as secondary metabolism and stress responses that affect plant growth and development. However, the regulatory roles of MYB-related genes in drought stress response remain unclear in maize. In this study, we discovered that a 1R-MYB gene, ZmRL6, encodes a 96-amino acid protein and is highly drought-inducible. We also found that it is conserved in both barley (Hordeum vulgare L.) and Aegilops tauschii. Furthermore, we observed that overexpression of ZmRL6 can enhance drought tolerance while knock-out of ZmRL6 by CRISPR-Cas9 results in drought hypersensitivity. DAP-seq analyses additionally revealed the ZmRL6 target genes mainly contain ACCGTT, TTACCAAAC and AGCCCGAG motifs in their promoters. By combining RNA-seq and DAP-seq results together, we subsequently identified eight novel target genes of ZmRL6 that are involved in maize's hormone signal transduction, sugar metabolism, lignin synthesis, and redox signaling/oxidative stress. Collectively, our data provided insights into the roles of ZmRL6 in maize's drought response.</p>","PeriodicalId":74874,"journal":{"name":"Stress biology","volume":"3 1","pages":"47"},"PeriodicalIF":0.0,"publicationDate":"2023-11-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10654321/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136400719","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Maleic acid and malonic acid reduced the pathogenicity of Sclerotinia sclerotiorum by inhibiting mycelial growth, sclerotia formation and virulence factors. 马来酸和丙二酸通过抑制菌丝生长、菌核形成和毒力因子来降低菌核菌的致病性。
Stress biology Pub Date : 2023-11-13 DOI: 10.1007/s44154-023-00122-0
Yu-Chen Fei, Qin Cheng, Huan Zhang, Chuang Han, Xu Wang, Yan-Feng Li, Shi-Qian Li, Xiao-Hu Zhao
{"title":"Maleic acid and malonic acid reduced the pathogenicity of Sclerotinia sclerotiorum by inhibiting mycelial growth, sclerotia formation and virulence factors.","authors":"Yu-Chen Fei, Qin Cheng, Huan Zhang, Chuang Han, Xu Wang, Yan-Feng Li, Shi-Qian Li, Xiao-Hu Zhao","doi":"10.1007/s44154-023-00122-0","DOIUrl":"10.1007/s44154-023-00122-0","url":null,"abstract":"<p><p>Sclerotinia sclerotiorum is a necrotrophic plant pathogenic fungus with broad distribution and host range. Bioactive compounds derived from plant extracts have been proven to be effective in controlling S. sclerotiorum. In this study, the mycelial growth of S. sclerotiorum was effectively inhibited by maleic acid, malonic acid, and their combination at a concentration of 2 mg/mL, with respective inhibition rates of 32.5%, 9.98%, and 67.6%. The treatment of detached leaves with the two acids resulted in a decrease in lesion diameters. Interestingly, maleic acid and malonic acid decreased the number of sclerotia while simultaneously increasing their weight. The two acids also disrupted the cell structure of sclerotia, leading to sheet-like electron-thin regions. On a molecular level, maleic acid reduced oxalic acid secretion, upregulated the expression of Ss-Odc2 and downregulated CWDE10, Ss-Bi1 and Ss-Ggt1. Differently, malonic acid downregulated CWDE2 and Ss-Odc1. These findings verified that maleic acid and malonic acid could effectively inhibit S. sclerotiorum, providing promising evidence for the development of an environmentally friendly biocontrol agent.</p>","PeriodicalId":74874,"journal":{"name":"Stress biology","volume":"3 1","pages":"45"},"PeriodicalIF":0.0,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643788/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89720972","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Do plant histone variants stand idly by while DNA viruses invade the nucleus? 当DNA病毒入侵细胞核时,植物组蛋白变体是否袖手旁观?
Stress biology Pub Date : 2023-11-13 DOI: 10.1007/s44154-023-00129-7
Zhihao Jiang, Rosa Lozano-Durán
{"title":"Do plant histone variants stand idly by while DNA viruses invade the nucleus?","authors":"Zhihao Jiang, Rosa Lozano-Durán","doi":"10.1007/s44154-023-00129-7","DOIUrl":"10.1007/s44154-023-00129-7","url":null,"abstract":"","PeriodicalId":74874,"journal":{"name":"Stress biology","volume":"3 1","pages":"46"},"PeriodicalIF":0.0,"publicationDate":"2023-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10643808/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89720971","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Transcriptomic analysis of wheat reveals possible resistance mechanism mediated by Yr10 to stripe rust. 小麦转录组分析揭示了Yr10介导的抗条锈病机制。
Stress biology Pub Date : 2023-10-23 DOI: 10.1007/s44154-023-00115-z
Zhongyi Wu, Gaohua Zhang, Ran Zhao, Qi Gao, Jinchen Zhao, Xiaoxu Zhu, Fangyan Wang, Zhensheng Kang, Xiaojing Wang
{"title":"Transcriptomic analysis of wheat reveals possible resistance mechanism mediated by Yr10 to stripe rust.","authors":"Zhongyi Wu, Gaohua Zhang, Ran Zhao, Qi Gao, Jinchen Zhao, Xiaoxu Zhu, Fangyan Wang, Zhensheng Kang, Xiaojing Wang","doi":"10.1007/s44154-023-00115-z","DOIUrl":"10.1007/s44154-023-00115-z","url":null,"abstract":"<p><p>Stripe rust, caused by Puccinia striiformis f. sp. tritici (Pst), is a catastrophic disease that threatens global wheat yield. Yr10 is a race-specific all-stage disease resistance gene in wheat. However, the resistance mechanism of Yr10 is poorly characterized. Therefore, to elucidate the potential molecular mechanism mediated by Yr10, transcriptomic sequencing was performed at 0, 18, and 48 h post-inoculation (hpi) of compatible wheat Avocet S (AvS) and incompatible near-isogenic line (NIL) AvS + Yr10 inoculated with Pst race CYR32. Respectively, 227, 208, and 4050 differentially expressed genes (DEGs) were identified at 0, 18, and 48 hpi between incompatible and compatible interaction. The response of Yr10 to stripe rust involved various processes and activities, as indicated by the results of Gene Ontology (GO) enrichment analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis. Specifically, the response included photosynthesis, defense response to fungus, metabolic processes related to salicylic acid (SA) and jasmonic acid (JA), and activities related to reactive oxygen species (ROS). Ten candidate genes were selected for qRT-PCR verification and the results showed that the transcriptomic data was reliable. Through the functional analysis of candidate genes by the virus-induced gene silencing (VIGS) system, it was found that the gene TaHPPD (4-hydroxyphenylpyruvate dioxygenase) negatively regulated the resistance of wheat to stripe rust by affecting SA signaling, pathogenesis-related (PR) gene expression, and ROS clearance. Our study provides insight into Yr10-mediated resistance in wheat.</p>","PeriodicalId":74874,"journal":{"name":"Stress biology","volume":"3 1","pages":"44"},"PeriodicalIF":0.0,"publicationDate":"2023-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10593697/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49694872","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Short wind pulses consistently change the morphology of roots, but not of shoots, across young plants of different growth forms. 在不同生长形式的幼苗中,短风脉冲会持续改变根的形态,但不会改变芽的形态。
Stress biology Pub Date : 2023-10-09 DOI: 10.1007/s44154-023-00123-z
Johannes Heinze, Luise Werger, Michael Ogden, Thilo Heinken, Rainer Hoefgen, Ewald Weber
{"title":"Short wind pulses consistently change the morphology of roots, but not of shoots, across young plants of different growth forms.","authors":"Johannes Heinze, Luise Werger, Michael Ogden, Thilo Heinken, Rainer Hoefgen, Ewald Weber","doi":"10.1007/s44154-023-00123-z","DOIUrl":"10.1007/s44154-023-00123-z","url":null,"abstract":"<p><p>Wind is an environmental stimulus that stresses plants of all growth forms at all life-stages by influencing the development, architecture, and morphology of roots and shoots. However, comparative studies are scarce and no study directly investigated whether shoot and root morphological traits of trees, grasses and forbs differ in their response to short wind pulses of different wind intensity. In this study, we found that across species, wind stress by short wind pulses of increasing intensity consistently changed root morphology, but did not affect shoot morphological traits, except plant height in four species. Wind effects in roots were generally weak in tree species but consistent across growth forms. Furthermore, plant height of species was correlated with changes in specific root length and average diameter.Our results indicate that short-pulse wind treatments affect root morphology more than shoot morphology across growth forms. They further suggest that wind stress possibly promotes root anchorage in young plants and that these effects might depend on plant height.</p>","PeriodicalId":74874,"journal":{"name":"Stress biology","volume":"3 1","pages":"43"},"PeriodicalIF":0.0,"publicationDate":"2023-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10562299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41165003","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
BAK-up: the receptor kinase BAK-TO-LIFE 2 enhances immunity when BAK1 is lacking. BAK-up:当BAK1缺乏时,受体激酶BAK-TO-LIFE 2增强免疫力。
Stress biology Pub Date : 2023-09-25 DOI: 10.1007/s44154-023-00124-y
Vahid Fallahzadeh-Mamaghami, Hannah Weber, Birgit Kemmerling
{"title":"BAK-up: the receptor kinase BAK-TO-LIFE 2 enhances immunity when BAK1 is lacking.","authors":"Vahid Fallahzadeh-Mamaghami, Hannah Weber, Birgit Kemmerling","doi":"10.1007/s44154-023-00124-y","DOIUrl":"10.1007/s44154-023-00124-y","url":null,"abstract":"<p><p>BRI1-ASSOCIATED KINASE 1 (BAK1/SERK3) and its closest homolog BAK1-LIKE 1 (BKK1/SERK4) are leucine-rich repeat receptor kinases (LRR-RKs) belonging to the SOMATIC EMBRYOGENESIS RECEPTOR KINASE (SERK) family. They act as co-receptors of various other LRR-RKs and participate in multiple signaling events by complexing and transphosphorylating ligand-binding receptors. Initially identified as the brassinosteroid receptor BRASSINOSTEROID INSENSITIVE 1 (BRI1) co-receptor, BAK1 also functions in plant immunity by interacting with pattern recognition receptors. Mutations in BAK1 and BKK1 cause severely stunted growth and cell death, characterized as autoimmune cell death. Several factors play a role in this type of cell death, including RKs and components of effector-triggered immunity (ETI) signaling pathways, glycosylation factors, ER quality control components, nuclear trafficking components, ion channels, and Nod-like receptors (NLRs). The Shan lab has recently discovered a novel RK BAK-TO-LIFE 2 (BTL2) that interacts with BAK1 and triggers cell death in the absence of BAK1 and BKK1. This RK compensates for the loss of BAK1-mediated pattern-triggered immunity (PTI) by activating phytocytokine-mediated immune and cell death responses.</p>","PeriodicalId":74874,"journal":{"name":"Stress biology","volume":"3 1","pages":"42"},"PeriodicalIF":0.0,"publicationDate":"2023-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10519891/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41168027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
TaNRAMP3 is essential for manganese transport in Triticum aestivum. TaNRAMP3对小麦中锰的转运至关重要。
Stress biology Pub Date : 2023-09-22 DOI: 10.1007/s44154-023-00120-2
Zhangqing Wang, Yanting Zhang, Chenyu Cao, Jiaming Liu, Yuan Deng, Zhenqian Zhang, Cun Wang
{"title":"TaNRAMP3 is essential for manganese transport in Triticum aestivum.","authors":"Zhangqing Wang, Yanting Zhang, Chenyu Cao, Jiaming Liu, Yuan Deng, Zhenqian Zhang, Cun Wang","doi":"10.1007/s44154-023-00120-2","DOIUrl":"10.1007/s44154-023-00120-2","url":null,"abstract":"<p><p>Manganese (Mn) is an essential trace element for almost all living organisms. In plants, Mn deficiency, which is occurs in calcareous soils or alkaline soils, severely limiting crop yields. However, the potential mechanism of Mn transport in Triticum aestivum is still obscure. Here, we found that TaNRAMP3, a member of the naturally resistant macrophage protein (NRAMP) family in Triticum aestivum, is located in the plasma membrane of protoplasts and functions as an influx transporter for Mn in yeast (Δsmf1). The expression of TaNRAMP3 was induced under Mn-deficiency conditions. Furthermore, TaNRAMP3-RNAi plants exhibited a sensitive phenotype, while transgenic plants overexpressing TaNRAMP3 showed a tolerant phenotype. In addition, TaNRAMP3 rescued the sensitive phenotype of Arabidopsis nramp1 mutant under Mn deficiency condition. In summary, our study reveals the key role of TaNRAMP3 in Mn transport in Triticum aestivum, allowing it to adapt to Mn-deficiency stress. These findings provide new insights for the cultivation of Mn-deficiency tolerant wheat varieties.</p>","PeriodicalId":74874,"journal":{"name":"Stress biology","volume":"3 1","pages":"41"},"PeriodicalIF":0.0,"publicationDate":"2023-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10516799/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41175137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Overwintering covered with soil or avoiding burial of wine grapes under cold stress: Chinese wine industry's past and future, challenges and opportunities. 冷胁迫下酒葡萄覆土越冬或避埋:中国葡萄酒产业的过去与未来、挑战与机遇。
Stress biology Pub Date : 2023-09-15 DOI: 10.1007/s44154-023-00119-9
Ningjing Wan, Bohan Yang, Dingze Yin, Tingting Ma, Yulin Fang, Xiangyu Sun
{"title":"Overwintering covered with soil or avoiding burial of wine grapes under cold stress: Chinese wine industry's past and future, challenges and opportunities.","authors":"Ningjing Wan, Bohan Yang, Dingze Yin, Tingting Ma, Yulin Fang, Xiangyu Sun","doi":"10.1007/s44154-023-00119-9","DOIUrl":"10.1007/s44154-023-00119-9","url":null,"abstract":"<p><p>In northwest China, where winter is extremely cold and the grapevine is vulnerable to freezing damage, the application of soil covering has promoted the vigorous development of the local grape and wine industries. However, in recent years, the negative effects of burying soil for cold protection on the environment have gradually emerged. In some viticultural regions, the phenomenon of \"summer forest, winter desert\" has appeared. Therefore, it is urgent for the Chinese grape industry to find a better solution to overwinter safely and environmentally friendly. This review summarizes the advantages and disadvantages of widely used solutions to overwinter such as covering vines with soil, breeding of cold-resistant grapes, cold-resistant cultivation model, physical and chemical covering materials, and protected grape facilities were reviewed. Future overwintering measures were proposed which avoid burial and grape overwintering research directions. It also provides a theoretical foundation and technical support to improve grape yield and quality in northwest China.</p>","PeriodicalId":74874,"journal":{"name":"Stress biology","volume":"3 1","pages":"40"},"PeriodicalIF":0.0,"publicationDate":"2023-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10504205/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10307037","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
iTRAQ-based protein profiling and functional identification of four genes involved in rice basal resistance against Magnaporthe oryzae in two contrasting rice genotypes. 基于itraq的水稻对稻瘟病基础抗性相关基因的蛋白谱分析和功能鉴定
Stress biology Pub Date : 2023-09-12 DOI: 10.1007/s44154-023-00118-w
Chenchen Li, Ziqiang Chen, Yun Deng, Shuyu Jiang, Yan Su, Shaohua Yang, Yan Lin, Dagang Tian
{"title":"iTRAQ-based protein profiling and functional identification of four genes involved in rice basal resistance against Magnaporthe oryzae in two contrasting rice genotypes.","authors":"Chenchen Li, Ziqiang Chen, Yun Deng, Shuyu Jiang, Yan Su, Shaohua Yang, Yan Lin, Dagang Tian","doi":"10.1007/s44154-023-00118-w","DOIUrl":"10.1007/s44154-023-00118-w","url":null,"abstract":"<p><p>Rice blast, caused by Magnaporthe oryzae, is one of the most destructive rice diseases. Developing blast-resistant rice cultivars represents the most economical and environmentally friend strategy for managing the disease. In our previous study, an isobaric tags for relative and absolute quantitation (iTRAQ)-based comparative protein quantification was carried out to investigate the resistance gene Piz-t gene-mediated resistance response to infection in two contrasting rice genotypes of the Piz-t transgenic Nipponbare line (NPB-Piz-t) and its wild-type Nipponbare (NPB). Here, from the comparisons of differentially expressed proteins (DEPs) of NPB-Piz-t to the avirulent isolate KJ201 (KJ201-Piz-t)and the virulent isolate RB22 (RB22-Piz-t) with mock-treated NPB-Piz-t (Mock-Piz-t), NPB to the virulent isolate KJ201(KJ201-NPB) and RB22 (RB22-NPB) with mock-treated NPB (Mock-NPB), 1, 1, and 6 common DEPs were, respectively, identified at 24, 48 and 72 h post-inoculation (hpi) in the susceptible comparisons of RB22-Pizt/Mock-Piz-t, KJ201-NPB/Mock-NPB, and RB22-NPB/Mock-NPB, involving in gi|54,290,836 and gi|59,800,021 were identified in the resistance comparison KJ201-Piz-t/Mock-Piz-t at 48 and 72 hpi respectively. Moreover, four genes of Os01g0138900 (gi|54,290,836), Os04g0659300 (gi|59,800,021), Os09g0315700 (gi|125,563,186) or Os04g0394200 (gi|21,740,743) were knocked out or overexpressed in NPB using gene over-expression and CRISPR/Cas9 technology, and results verified that the Os01g0138900 obviously affected the rice blast resistance. Further, expression and targeted metabolomics analysis illuminated the resistance response of cysteine-containing substances as gi|59,800,021 under blast infection. These results provide new targets for basal resistance gene identification and open avenues for developing novel rice blast resistant materials.</p>","PeriodicalId":74874,"journal":{"name":"Stress biology","volume":"3 1","pages":"39"},"PeriodicalIF":0.0,"publicationDate":"2023-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10497467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10240126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Collaborative impact of bacterial exometabolites governing root microbiota formation. 细菌外代谢产物对根微生物群形成的协同影响。
Stress biology Pub Date : 2023-09-07 DOI: 10.1007/s44154-023-00121-1
Hafiz Abdul Kareem, Xinwei Hao, Xihui Shen
{"title":"Collaborative impact of bacterial exometabolites governing root microbiota formation.","authors":"Hafiz Abdul Kareem, Xinwei Hao, Xihui Shen","doi":"10.1007/s44154-023-00121-1","DOIUrl":"10.1007/s44154-023-00121-1","url":null,"abstract":"<p><p>The majority of the root microbiota formation derives from soil-dwelling microorganisms. The limited extent of thorough investigation leads to a dearth of knowledge concerning the intricate mechanisms of microbe-microbe interaction implicated in the establishment of root microbiota. Therefore, the taxonomic signatures in bacterial inhibition profiles were determined by in vitro testing of 39,204 binary interbacterial interactions. However, findings from genetic and metabolomic studies elucidated that co-functioning of the antimicrobial 2,4-d iacetylphloroglucinol (DAPG) and the iron chelator pyoverdine as exometabolites has significantly contributed to the potent inhibitory activities of the highly antagonistic Pseudomonas brassicacearum R401. Microbiota restoration with a core of Arabidopsis thaliana root commensals showed that these exometabolites possess a root niche-specific function in establishing root competence and inducing anticipated changes in root surroundings. Both biosynthetic operons are abundant in roots in natural habitats, indicating that these exometabolites co-functioning is an adaptive feature that helps Pseudomonad dominate the root microbiota.</p>","PeriodicalId":74874,"journal":{"name":"Stress biology","volume":"3 1","pages":"38"},"PeriodicalIF":0.0,"publicationDate":"2023-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10484853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10188637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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