Advances in Virology最新文献

{"title":"Study on Seroprevalence and Associated Risk Factors of Newcastle Disease in Smallholder Poultry Farms in Sodo Zuria District, Wolaita Zone, Southern Ethiopia.","authors":"Saliman Aliye,&nbsp;Habtamu Endale,&nbsp;Mesfin Mathewos,&nbsp;Haben Fesseha","doi":"10.1155/2022/7478018","DOIUrl":"https://doi.org/10.1155/2022/7478018","url":null,"abstract":"<p><p>Newcastle disease (NCD) is a highly contagious viral disease of poultry and remains a constant threat in poultry farms that causes huge economic losses. The objective of this study was to estimate and assess the seroprevalence and associated risk factors of Newcastle disease in the Sodo Zuria district, southern Ethiopia. A cross-sectional survey followed by a simple random sampling technique was conducted from May to July 2021 on 384 apparently healthy nonvaccinated chickens on 30 smallholder poultry farms using commercial indirect ELISA kits and a questionnaire survey. The data were analyzed by using STATA for windows version 20 and a logistic regression reporting odds ratiowas applied to describe the seroprevalence of Newcastle disease with associated risk factors. The result of the study demonstrates that there was a high seroprevalence 48.7% (<i>n</i>= 187/384) of Newcastle disease in the study district. Information on associated risk factors were assessed using a semistructured questionnaire. The sex of the chicken showed a statistically significant difference (<i>x</i> ² = 4.842; <i>p</i> = 0.028) with the seroprevalence of the disease. The difference in seroprevalence among intensive, semi-intensive, and extensive management system was statistically significant (<i>x</i> ² = 3.84; <i>p</i> = 0.0001). There was also a statistical significant difference (<i>x</i> ² = 2.3854; <i>p</i> = 0.496) in the absence and presence of safe disposal of a dead chicken with the occurence of Newcastle disease. However, no statistically significant difference was observed among age groups (<i>x</i> ² = 4.335; <i>p</i> = 0.114), disinfection of poultry house (<i>x</i> ² = 0.0; <i>p</i> = 0.998), presence and absence of footbath (<i>x</i> ² = 2.969; <i>p</i> = 0.085), the breeds (<i>x</i> ² = 4.490; <i>p</i> = 0.106), type of chicken (<i>x</i> ² = 0.302; <i>p</i> = 0.583), and housing system (<i>x</i> ² = 1.926; <i>p</i> = 0.588). A high seroprevalence without vaccination history showed that the virus was circulating within the poultry. Therefore, further molecular study has to be conducted to identify circulating strains and develop an evidence-based control program.</p>","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9519288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40393791","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The BioFire® RP2.1 Panel Did Not Identify Concurrent Respiratory Virus Infection in Adults with Variable SARS-CoV-2 Disease Severity and Infection Duration.","authors":"Kendra M Quicke,&nbsp;Bridget A Baxter,&nbsp;Sophia Stromberg,&nbsp;Emily N Gallichotte,&nbsp;Emily Fitzmeyer,&nbsp;Michael C Young,&nbsp;Kristy L Pabilonia,&nbsp;Nicole Ehrhart,&nbsp;Julie Dunn,&nbsp;Gregory D Ebel,&nbsp;Elizabeth P Ryan","doi":"10.1155/2022/1378482","DOIUrl":"https://doi.org/10.1155/2022/1378482","url":null,"abstract":"<p><p>SARS-CoV-2 emerged in 2019 and rapidly surged into a global pandemic. The rates of concurrent infection with other respiratory pathogens and the effects of possible coinfections on the severity of COVID-19 cases and the length of viral infection are not well defined. In this retrospective study, nasopharyngeal swab samples collected in Colorado between March 2020 and May 2021 from SARS-CoV-2 PCR-positive individuals were tested for a panel of 21 additional respiratory pathogens, including 17 viral and 4 bacterial pathogens. We detected significant positive correlations between levels of SARS-CoV-2 RNA and infectious virus titers for both cohorts, as well as a positive correlation between viral RNA levels and disease severity scores for one cohort. We hypothesized that severe COVID-19 cases and longer SARS-CoV-2 infections may be associated with concurrent respiratory infections. Only one individual exhibited evidence of a concurrent infection- SARS -CoV-2 and human rhinovirus/enterovirus- leading us to conclude that viral respiratory coinfections were uncommon during this time and thus not responsible for the variations in disease severity and infection duration observed in the two cohorts examined. Mask wearing and other public health measures were imposed in Colorado during the time of collection and likely contributed to low rates of coinfection.</p>","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9377931/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40422466","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Seroprevalence of COVID-19 in Blood Donors: A Systematic Review and Meta-Analysis.","authors":"Hassan Nourmohammadi,&nbsp;Ali Hasanpour Dehkordi,&nbsp;Amir Adibi,&nbsp;Seyed Mohammad Amin Hashemipour,&nbsp;Mohsen Abdan,&nbsp;Moloud Fakhri,&nbsp;Zahra Abdan,&nbsp;Diana Sarokhani","doi":"10.1155/2022/9342680","DOIUrl":"10.1155/2022/9342680","url":null,"abstract":"<p><strong>Introduction: </strong>Determining the prevalence of SARS-CoV-2 in blood donors makes the control of virus circulation possible in healthy people and helps implement strategies to reduce virus transmission. The purpose of the study was to examine the seroprevalence of COVID-19 in blood donors using systematic review and meta-analysis.</p><p><strong>Materials and methods: </strong>The electronic databases PubMed, Scopus, Web of Science, and the Google Scholar search engine were searched using standard keywords up to 2022-04-26. The variance of each study was calculated according to the binomial distribution. Studies were combined according to the sample size and variance. <i>Q</i> Cochrane test and I2 index were used to examine the heterogeneity of the studies. Data analysis was performed in STATA 14 software, and the significance level of the tests was <i>P</i> < 0.05.</p><p><strong>Results: </strong>In the 28 papers examined with 227894 samples, the seroprevalence of COVID-19 in blood donors was 10% (95% CI: 9%, 11%), estimated 5% (95% CI: 4%, 7%) among men and 6% (95% CI: 4%, 7%) among women. This rate in different blood groups was as follows: A 12% (95% CI: 10%-14%), B 12% (95% CI: 10%-15%), AB 9% (95% CI: 7%-12%), and O 13% (95% CI: 11%-16%). The seroprevalence of COVID-19 in blood donors in North America 10%, Europe 7%, Asia 23%, South America 5%, and Africa was 4%; Moreover, the seroprevalence of IgG antibodies was estimated to be 23% (95% CI: 18%-29%) and IgM 29% (95% CI: 9%-49%).</p><p><strong>Conclusion: </strong>The highest prevalence of COVID-19 serum in women blood donors was among blood group O and Asia. The seroprevalence of IgG and IgM antibodies was high too.</p>","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9334089/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40589863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Unique Trinucleotide-Bloc Mutation-Based Two SARS-CoV-2 Genotypes with Potential Pathogenic Impacts.","authors":"Mustak Ibn Ayub","doi":"10.1155/2022/5618222","DOIUrl":"https://doi.org/10.1155/2022/5618222","url":null,"abstract":"<p><p>SARS-CoV-2, the novel coronavirus behind the COVID-19 pandemic, is acquiring new mutations in its genome. Although some mutations provide benefits to the virus against human immune response, others may result in their reduced pathogenicity and virulence. By analyzing more than 3000 high-coverage, complete sequences deposited in the GISAID database up to April 2020, here I report the uniqueness of the 28881-28883: GGG > AAC trinucleotide-bloc mutation in the SARS-CoV-2 genome that results in two substrains, described here as SARS-CoV-2g (28881-28883: GGG genotype) and SARS-CoV-2a (28881-28883: AAC genotype). Computational analysis and literature review suggest that this bloc mutation would bring 203-204: RG (arginine-glycine)>KR (lysine-arginine) amino acid changes in the nucleocapsid (N) protein affecting the SR (serine-arginine)-rich motif of the protein, a critical region for the transcription of viral RNA and replication of the virus. Thus, 28881-28883: GGG > AAC bloc mutation is expected to modulate the pathogenicity of SARS-CoV-2. These analyses suggest that SARS-CoV-2 has evolved into SARS-CoV-2a affecting COVID-19 infectivity and severity. To confirm these assumptions, retrospective and prospective epidemiological studies should be conducted in different countries to understand the course of pathogenicity of SARS-CoV-2a and SARS-CoV-2g. Laboratory research should focus on the bloc mutation to understand its true impacts on the course of the pandemic. Potential drug and vaccine development should also keep the 28881-28883 region of the N protein under consideration.</p>","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9314171/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40553509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of Early Favipiravir Therapy in Hospitalised COVID-19 Patients.","authors":"Abdulrahman Tawfik,&nbsp;Abdulrahman Alzahrani,&nbsp;Sami Alharbi,&nbsp;Jamal Almitairi,&nbsp;Arwa Alzahrani,&nbsp;Mohammed Ali Alshehri,&nbsp;Mohammed S Aldughaim,&nbsp;Hani Alothaid","doi":"10.1155/2022/9240941","DOIUrl":"https://doi.org/10.1155/2022/9240941","url":null,"abstract":"<p><p>COVID-19 is a disease caused by a novel coronavirus with no specific, standard treatment. We investigated the clinical data of COVID-19 patients admitted to King Fahad Specialist Hospital (KFSH) in Buraydah by comparing the patients who were treated early with favipiravir (within 3 days of admission) to patients who were treated after three days of admission or not treated. 165 patients were confirmed with PCR tests and admitted to KFSH for treatment. Comorbidities contributed significantly to increasing the length of stay in hospital at 11.4 ± 0.8 days compared to patients with no comorbidities at 8.6 ± 0.9 days (<i>p</i>=0.041). A total of 103 patients were treated with favipiravir, and we found that early treatment with favipiravir (within 3 days) reduced the length of stay in hospital significantly (8.8 ± 1.4 days) compared to patients who were treated after 3 days (13.3 ± 4.6 days) (<i>p</i>=0.0015). Moreover, patients with comorbidities in both early and late treatment groups had significantly higher average lengths of stay in hospital (11.2 ± 0.9 days) compared to patients with no comorbidities (7.9 ± 0.7 days) (<i>p</i>=0.017). Interestingly, patients treated early with favipiravir (with comorbidities and without) stayed fewer days in hospital compared to those with late treatment (<i>p</i>=0.021; a difference of 4.5 ± 1.9 days; and <i>p</i>=0.018; a difference of 4.2 ± 1.7 days, respectively). In conclusion, our analysis indicates that early treatment with favipiravir can reduce the length of stay in hospital and improve clinical manifestations of COVID-19 patients.</p>","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9259373/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40578232","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Confirmatory Virucidal Activity of Ionised Active Water S-100® on the SARS-CoV-2 Virus.","authors":"Nathalie Wurtz,&nbsp;Issam Hasni,&nbsp;Audrey Bancod,&nbsp;Bernard La Scola","doi":"10.1155/2022/5995775","DOIUrl":"https://doi.org/10.1155/2022/5995775","url":null,"abstract":"<p><p>Ionised active water S-100® has been proposed as an original solution for use in dermocosmetics and for the treatment of wounds such as burns and atopic dermatitis. Among the mechanisms of action that are not completely understood, an antimicrobial activity would appear to be important. In the context of the COVID-19 pandemic, we assessed the inactivating efficacy of this solution on SARS-CoV-2 based on the recommendations of the NF-EN-14476+A2 standard. The tests carried out demonstrated that ionised active water S-100® 40% has a virucidal activity on SARS-CoV-2 which is at least 3.1 log after a contact time of 30 seconds and 3.5 log after two minutes at 20°C under clean conditions. Assays were also performed at 4°C and 37°C, and the results obtained are identical to those obtained at 20°C. This demonstration of the virucidal effect of ionised water against SARS-CoV-2 paves the way for the development of usage as an alternative disinfectant in SARS-CoV-2 control.</p>","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9232342/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40402094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Anti-Cytokine Storm Activity of Quercetin Zinc and Vitamin C Complex.","authors":"Hayder B Sahib,&nbsp;Omer Abid Kathum,&nbsp;Rafal Shakeeb Alanee,&nbsp;Rehab A M Jawad,&nbsp;Ahmed Majeed Al-Shammari","doi":"10.1155/2022/1575605","DOIUrl":"https://doi.org/10.1155/2022/1575605","url":null,"abstract":"<p><p>Cytokine storm is one of the causative deaths in a patient with severe acute respiratory syndrome. This study aimed at evaluating the prophylaxis effect of quercetin complexes with zinc and buffered ascorbic acid upon cytokine storm induction in mice and identifying the complex's acute toxicity. Mice were randomly divided into three groups: group A, control group, received 0.9% normal saline; group B received 100 mg/kg of the complex one hour before lipopolysaccharide (LPS) administration; and group C received the LPS IP 5 mg/kg. Then, levels of interleukin 1 and interleukin 6 were measured in the serum, and lung and kidney tissues were investigated for any changes that may have happened. Thirty mice were used to investigate the acute toxicity; mice were distributed into six groups: one control group and five treated groups; then several serial dilutions from the complex have been prepared for different concentrations from 5 g/kg to 0.312 g/kg. The animals were observed for 14 days. The LD₅₀ was deduced by the straight-line equation calculated from the dose-response curve. The results in this study showed that group A had no significant tissue change. LPS group C showed tissue damage in the lung and kidney, which significantly prevented by the pretreated complex in group B. Moreover, the complex's acute toxicity value (LD₅₀) was 655 mg/kg. In conclusion, the complex has significantly ameliorated LPS-induced acute lung and kidney injury, largely through suppression of inflammation; the large lethal dose value may make the complex have a promising therapeutic effect in the prevention of cytokine storm.</p>","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9200564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40041571","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Partial Analysis of the Capsid Protein (VP1) of Human Sapovirus Isolated from Children with Diarrhoea in Rural Communities of South Africa","authors":"Mpho Magwalivha, J. K. Ngandu, A. Traoré, N. Potgieter","doi":"10.1155/2022/9928378","DOIUrl":"https://doi.org/10.1155/2022/9928378","url":null,"abstract":"Background Viral diarrhoea is a concern in acute gastroenteritis cases among children younger than 5 years of age. Sapovirus has been noted as an emerging causative agent of acute gastroenteritis worldwide. Objective/Study Design. The aim of this study was to characterize human sapoviruses targeting the VP1 (NVR and N-terminal) region. Twenty-five samples were randomly selected from 40 sapovirus-positive samples previously detected and analyzed for the VP1 region using the One-Step RT-PCR assay. The PCR products were subjected to Sanger sequencing analysis. Results The polyprotein segment (NVR and N-terminal) was successfully amplified from 10/25 samples. Sapovirus GI.1 was the most predominant strain (6/10; 60%), followed by SV-GII.1 (2/10; 20%) and 10% of each GI.3 and GII.3. Conclusion Through the partial analysis of the VP1 region, this study provides more data to add on the human sapovirus genetic characterization of circulating strains in South Africa, with the proposition of further analysis of sapovirus VP1 fragments for the viral structure and function.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47688527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of Thermal Pretreatment of Saliva on the RT-PCR Detection of SARS-CoV-2","authors":"O. Morais, Manuel Rui Azevedo Alves, Paulo Fernandes","doi":"10.1155/2022/7442907","DOIUrl":"https://doi.org/10.1155/2022/7442907","url":null,"abstract":"The use of saliva directly as a specimen to detect viral RNA by RT-PCR has been tested for a long time as its advantages are relevant in terms of convenience and costs. However, as other body fluids, its proven inhibition effect on the amplification reaction can be troublesome and compromise its use in the detection of viral particles. The aim of the present work is to demonstrate that saliva pretreatment may influence the RT-PCR amplification of three gene targets of SARS-CoV-2 significantly. A pool of RNA from confirmed COVID-19 patients was used to test the influence of heat pretreatment of saliva samples at 95°C for 5, 10, 15 and 20 min on the amplification performance of ORF1ab, E, and N SARS-CoV-2 genes. Prolonged heating at 95°C significantly improves the Ct value shift, usually observed in the presence of saliva, increasing the limit of detection of viral genes ORF1ab, E, and N. When tested using a cohort of COVID-19 patients' saliva, the increased time of heat pretreatment resulted in a significant increase in the detection sensitivity.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44553794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Performance Evaluation of FilmArray BioFire RP2.1 and MAScIR 2.0 Assays for SARS-CoV-2 Detection","authors":"S. Tazi, H. Kabbaj, Jalila Zirar, Amal Zouaki, Ghizlane El Amin, Othman El Himeur, M. Seffar","doi":"10.1155/2022/4510900","DOIUrl":"https://doi.org/10.1155/2022/4510900","url":null,"abstract":"Background RT-PCR is the gold standard for COVID-19 diagnosis, but the lack of standardization of assays, whose diagnostic performance may widely vary, complicates the interpretation of the discrepancies that may be encountered. Study design. We conducted a retrospective study over a ten-month period at the Central Laboratory of Virology of Ibn Sina University Hospital of Rabat. We included nasopharyngeal swabs, positive and negative for SARS-CoV-2 on FilmArray BioFire® Respiratory Panel 2.1 Plus, which were subjected to our laboratory's reference test, MAScIR SARS-CoV-2 M kit 2.0, initially or after a freeze-thaw cycle. The results were compared, and each discrepant sample with sufficient volume underwent the third test, using ARGENE® SARS-CoV-2 R-GENE kit. Results Of 80 SARS-CoV-2 negative samples on FilmArray, there were no discordant results, whereas of 80 SARS-CoV-2 positive samples on FilmArray, 21 had discordant results on MAScIR, and only 11 could be tested on ARGENE, revealing positive results in 6 cases. 12.7% and 76.5% correspond to the discordance rates for MAScIR (with one or both targets detected on FilmArray), while 14.3% and 100% correspond to those of ARGENE. As the estimated sensitivity and specificity of FilmArray, compared with MAScIR, were 100% and 79.2%, respectively, its lower limit of detection, and ARGENE assay results, made it difficult to distinguish between false positives on FilmArray and false negatives on MAScIR without further investigations. Conclusion The implementation of a new assay in our laboratory revealed discrepancies suggesting a lack of sensitivity of our laboratory's reference test, leading us consequently to retain the SARS-CoV-2 positive result of these discordant samples on FilmArray, regardless of the detection of one or both targets. Our study, which is, to our knowledge, the first comparing FilmArray RP2.1 and MAScIR 2.0 assays for SARS-CoV-2 detection, highlights the urgent need to standardize RT-PCR assays for COVID-19 diagnosis.","PeriodicalId":7473,"journal":{"name":"Advances in Virology","volume":null,"pages":null},"PeriodicalIF":2.2,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46257292","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}