Reproduction and breeding最新文献

{"title":"Study on sperm cryopreservation of hybrid fish derived from Carassius cuvieri (♀) × Carassius auratus red var (♂)","authors":"Qianqian Zeng ,&nbsp;Yixuan Chen ,&nbsp;Minyi Wang ,&nbsp;Yinggang Li ,&nbsp;Tao Dai ,&nbsp;Weiling Qin ,&nbsp;Yating Zhu ,&nbsp;Chun Zhang ,&nbsp;Yi Zhou ,&nbsp;Qinbo Qin ,&nbsp;Conghui Yang ,&nbsp;Qianhong Gu","doi":"10.1016/j.repbre.2024.09.002","DOIUrl":"10.1016/j.repbre.2024.09.002","url":null,"abstract":"<div><div>Cryopreservation of sperm is an effective method for conserving germplasm resources in fish genetic breeding. The high-quality hybrid fish (WR) derived from white crucian carp (<em>Carassius cuvieri</em>, WCC, ♀) and red crucian carp (<em>C</em>. <em>auratus</em> red var., RCC, ♂), possesses valuable traits such as high survival rates, strong resistance, and rapid growth, representing an important germplasm resources of crucian carp. This study compared the effects of different antifreeze solutions on sperm viability among three varieties (WR, WCC, and RCC) and examined changes in enzyme activity, fertilization rates, and hatching rates after cryopreservation, aiming to enhance the cryogenic sperm cryopreservation technique in hybrid fish and investigate the mechanisms underlying spermatozoa damage caused by cryopreservation. The results showed that the antifreeze combination of D14 with 15 % dimethyl sulfoxide (DMSO) had the best effect in preserving the sperm of WR and WCC, while D20 with 10 % DMSO was the optimal combination for RCC sperm. After ultra-low temperature preservation, the longevity, fertilization, and hatching rates of frozen sperm were significantly lower (<em>P</em> &lt; 0.05) compared to fresh sperm. The enzyme activities of superoxide dismutase (SOD), lactate dehydrogenase (LDH), and creatine kinase (CK) were significantly decreased (<em>P</em> &lt; 0.05) in spermatozoa, whereas they showed a significant increase (<em>P</em> &lt; 0.05) in sperm plasma. Succinate dehydrogenase (SDH) activity was significantly reduced (<em>P</em> &lt; 0.05) in frozen spermatozoa of RCC compared to fresh spermatozoa, and exhibited lower activity in frozen spermatozoa of WCC and WR. Additionally, SDH activity was significantly elevated (<em>P</em> &lt; 0.05) in frozen sperm plasma, and glutathione reductase (GR) activity was significantly lower (<em>P</em> &lt; 0.05) in both frozen sperm plasma and spermatozoa across all three species. The study demonstrated that cryopreservation had a significant effect on the enzyme activities of spermatozoa and sperm plasma in all three species. These findings provide important technical support for the conservation of high-quality fish germplasm resources, particularly for novel varieties resulting from distant hybridization in fish.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 4","pages":"Pages 234-242"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The expression and function of gpr54a and gpr54b in allotriploid crucian carp and diploid red crucian carp (Carassius auratus red var.)","authors":"Lu Huang ,&nbsp;Qiubei Wang ,&nbsp;Shuxin Zhang,&nbsp;Faxian Yu,&nbsp;Shengnan Li,&nbsp;Huan Zhong,&nbsp;Rurong Zhao,&nbsp;Min Tao","doi":"10.1016/j.repbre.2024.08.004","DOIUrl":"10.1016/j.repbre.2024.08.004","url":null,"abstract":"<div><div>The kisspeptin receptor (GPR54), a member of the G-protein-coupled receptors, plays a central role in regulating reproduction. However, research on GPR54 in allotriploid fish remains limited. In this study, we reported the full-length cDNAs, tissue expression, and localization of <em>gpr54a</em> and <em>gpr54b</em> in allotriploid crucian carp (ACC) and diploid red crucian carp (<em>Carassius auratus</em> red var., RCC). The full-length cDNAs of <em>gpr54a</em> in ACC and RCC were 2224 bp and 2218 bp, respectively. The full-length cDNAs of <em>gpr54b</em> in ACC and RCC were 1413 bp and 1182 bp, respectively. qRT-PCR revealed that ACC <em>gpr54a</em> and RCC <em>gpr54a</em> were highly expressed in the brain but had lower expression in peripheral tissues. Unlike <em>gpr54a</em>, ACC <em>gpr54b</em> and RCC <em>gpr54b</em> were highly expressed in the brain, liver, and muscle. During the breeding season, the expression of <em>gpr54a</em> in ACC was significantly lower than in RCC, but <em>gpr54b</em> had significantly higher expression in ACC than in RCC in the HPG axis. This finding suggested that <em>gpr54a</em> might promote ovarian development, while <em>gpr54b</em> might inhibit ovarian development. During the breeding season, ISH indicated abnormal ovarian development in ACC and found signals of <em>gpr54a</em> and <em>gpr54b</em> in several regions of the brain, pituitary, and ovary of ACC and RCC. These regions of the brain, pituitary, and ovary are associated with the secretion of several hormones related to reproductive regulation. Hence, we hypothesized that the lower expression of <em>gpr54a</em> and higher expression of <em>gpr54b</em> in ACC might be involved in sterility by regulating the secretion of reproductive hormones. This study suggested that <em>gpr54a</em> and <em>gpr54b</em> might function oppositely in regulating reproduction. In conclusion, these findings will aid in further investigating the functions of GPR54 and provide a theoretical basis for studying ACC sterility.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 4","pages":"Pages 221-233"},"PeriodicalIF":0.0,"publicationDate":"2024-09-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142323663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Identification of key genes affecting development and nutrient metabolism in the early seedling stage of Dongting catfish (Silurus asotus)","authors":"Yanfang Wu ,&nbsp;Jiaxin Fu ,&nbsp;Jixiang Chu ,&nbsp;Jun Yan ,&nbsp;Jun Xiao ,&nbsp;Can Yang ,&nbsp;Rui Song ,&nbsp;Hao Feng","doi":"10.1016/j.repbre.2024.08.003","DOIUrl":"10.1016/j.repbre.2024.08.003","url":null,"abstract":"<div><p>As a carnivorous fish, the cannibalism are prone to happen in the early seedling stage in Dongting catfish (<em>Silurus asotus</em>), especailly when there is not enough food, which resulting in the low survival rate of Dongting catfish. To find clues to improve the survival rate of Dongting catfish offspring, the comparative transcriptomics analysis were conducted between normal and malformed larval fish, as well as normal larval fish before and after first food intake. In normal and malformed larval fish groups, a total of 881 differential genes were identified, including 634 up-regulated genes and 247 down-regulated genes. From these genes related to growth hormone synthesis and secretion were screened out as <em>SLA isoform X1</em>, <em>SOCS3</em>, <em>STAT1b</em>, <em>JUNB</em>, respectively. In the group of larval fish before and after the first food intake, 4901 up-regulated genes and 3660 down-regulated genes were found, from which the differential genes related to protein digestion and absorption pathway and pancreatic secretion pathway were screened out as <em>cathepsin D precursor</em>, <em>elastase 2 like precursor</em>, <em>elastase 2 precursor</em>, <em>LPL isoform X1</em>, <em>CTRB1 precursor</em>, <em>CTRL precursor</em>, <em>CPA precursor</em>, <em>FABP</em>, <em>SLC15A1</em>, <em>CEL tandem duplicate 2 precursor</em>. The differential genes screened for association with amino acid metabolism were <em>ASAOC precursor</em>, <em>PLD3</em>, <em>NDPKs</em>, <em>trehalase</em>, <em>UMP-CMP kinase</em>, <em>L-amino-acid oxidase</em>, <em>PLB1</em>, <em>FALDH isoform X2</em>, <em>PLA2 precursor</em>, <em>glucokinase</em>. Meanwhile, nine differential genes were selected for qPCR verification. The results confirmed that the relative expression trends of these genes were consistent with those found in the transcriptome. These findings provide an important reference for developing strategies to enhance the survival rate of Dongting catfish at early seedling stage.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 4","pages":"Pages 212-220"},"PeriodicalIF":0.0,"publicationDate":"2024-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071224000395/pdfft?md5=797272dc11a4ae7f78ab3a11f8687edc&pid=1-s2.0-S2667071224000395-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142137141","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic performance, heritability, and correlation of traits in new plant type of rice lines for highland ecosystem","authors":"Yusuf Limbongan ,&nbsp;Rinaldi Sjahril ,&nbsp;Adewidar Marano Pata'dungan ,&nbsp;Trisday Yiin Parari","doi":"10.1016/j.repbre.2024.08.002","DOIUrl":"10.1016/j.repbre.2024.08.002","url":null,"abstract":"<div><p>Rice cultivation is crucial in providing global food needs, particularly in highland areas with distinct environmental conditions. Adapting promising rice lines suitable for highland ecosystems is essential to ensure sustainable rice production in such regions. This study assesses the agronomic performance, heritability, and correlation of traits in new promising rice lines adapted to highland ecosystems. The latest promising rice lines are designed with the unique environmental conditions of highland regions in mind. This research was conducted in Buntu Barana’ Village, Tikala Sub-district, with an elevation of 858.4 m above sea level. The study was carried out in rain-fed semi-irrigated paddy fields. The research was conducted from May 2023 to January 2024<strong>.</strong> The research methodology involves testing various agronomic traits for yield production components of the new type of promising rice lines at different highland locations. The research procedures included the selection of viable seed strains, seeding, fertilization, balanced irrigation, control of plant pest organisms, harvesting, and post-harvesting. Evaluated agronomic traits encompass plant height, days to maturity, number of productive tillers, length of panicles, number of filled grains per panicle, and grain yield. Additionally, heritability analysis is performed to assess the extent to which observed trait variations are attributed to genetic factors. Research findings reveal significant variations in agronomic traits among the new type of promising rice lines. The highest grain yield is achieved in genotype UKIT102-2-056, which is 8.3 tons per hectare of paddy rice. These lines exhibit objective adaptation to the highland ecosystem, with some lines demonstrating superior performance in specific parameters, especially early maturity, namely 97 days after planting. Heritability analysis indicates that certain traits have high heritability levels, suggesting a significant role of genetic factors in controlling these traits. Furthermore, this study unveils correlation patterns among the observed agronomic traits. Significant positive and negative correlations among several traits indicate complex interrelationships among these agronomic characteristics. These findings offer further insights into the relationships between these traits and can be utilized to develop more adaptive rice lines in highland environments.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 4","pages":"Pages 203-211"},"PeriodicalIF":0.0,"publicationDate":"2024-08-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071224000383/pdfft?md5=8f5a702cef97ef2da8d78f31ec2c5c79&pid=1-s2.0-S2667071224000383-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142094776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Multivariate analysis and image-based phenotyping of cayenne fruit traits in selection and diversity mapping of multiple F1 cross lines","authors":"Muhammad Fuad Anshori ,&nbsp;Yunus Musa ,&nbsp;Novaty Eny Dungga ,&nbsp;Nuniek Widiayani ,&nbsp;Arfina Sukmawati Arifin ,&nbsp;A. Masniawati ,&nbsp;Firmansyah ,&nbsp;Muh Farid ,&nbsp;Andi Dirpan ,&nbsp;Andi Isti Sakinah ,&nbsp;Nirwansyah Amier","doi":"10.1016/j.repbre.2024.08.001","DOIUrl":"10.1016/j.repbre.2024.08.001","url":null,"abstract":"<div><p>The phenomenon of fluctuating chili prices can be resolved in stages, one of which is through multiple crosses. However, this cross requires precise methods in the evaluation and selection process, especially regarding fruit characteristics. Image-based phenotyping 4.0 approaches can increase the potential precision of such evaluation genotypes, especially when this approach is combined with multivariate analysis. Therefore, both methods are needed to evaluate and select these cayenne multiple crosses. This research aims to identify the effectiveness of multivariate analysis and image-based explanatory characteristics of fruit phenotypes and to select multiple crosses that can continue to the F2 generation. The research was designed with a randomized complete block design of ten F1 multiple cross-genotypes and four check varieties. Each genotype was repeated three times, so there were 42 experimental units. Based on the results, multivariate was considered adequate in determining image explanatory characters based on fruit phenotype and genotype mapping of the population diversity of multiple crosses of cayenne pepper. The characteristics of fruit height, fruit area, and fruit Intden are image-based explanatory characters that can map the completeness of cayenne pepper fruit between multiple crosses well. This indicates that image-based phenotyping and multivariate analysis can provide more detailed image information of the potential of cayenne fruit from multiple crosses than just based on fruit weight. Therefore, both approaches are recommended for analyzing cayenne paper fruit potential, especially for multiple crosses. In addition, three crosses (MC4, MC8, and MC9) are optimal for the next generation to be recommended and continued.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 4","pages":"Pages 194-202"},"PeriodicalIF":0.0,"publicationDate":"2024-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071224000371/pdfft?md5=3375a5b839e582e52c0fa9c84f33faf8&pid=1-s2.0-S2667071224000371-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142012064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Population genetic characteristics of two crucian carp varieties derived from distant hybridization","authors":"Wenjie Luo ,&nbsp;Xuexue Huang ,&nbsp;Xiaowei Xu ,&nbsp;Chenghua Dai ,&nbsp;Qiong Liu ,&nbsp;Yating Zhu ,&nbsp;Duansheng Wu ,&nbsp;Shi Wang ,&nbsp;Qingfeng Liu ,&nbsp;Conghui Yang","doi":"10.1016/j.repbre.2024.07.002","DOIUrl":"10.1016/j.repbre.2024.07.002","url":null,"abstract":"<div><p>The Hefang crucian carp (HFJ) derived from <em>Carassius cuvieri</em> (♀) × <em>C. auratus</em> red var. (♂) and the crucian carp-like homodiploid fish (NCRC) derived from <em>Cyprinus carpio</em> (♀) × <em>Megalobrama amblycephala</em> (♂) are important germplasm resources of crucian carp. To investigate the population characteristics and reveal the current genetic situations of these hybrid varieties, we sequenced one nuclear molecular marker <em>transferrin</em> (<em>Tf</em>) among six <em>Carassius</em> populations, which included two hybrid varieties (HFJ and NCRC), one laboratory variety (red crucian carp, RCC) and three wild populations. A total of 166 distinct <em>Tf</em> alleles (A1∼A166) were identified, with sequence lengths ranging from 1145 bp to 1244 bp. Unexpectedly, the nucleotide diversity of <em>Tf</em> alleles in cultured populations (HFJ, NCRC, and RCC) exceeded that of wild populations. The phylogenetic reconstruction analysis results showed that HFJ and <em>C. cuvieri</em> were closely related, but they could not be separated from <em>C. auratus</em>. Besides, while most <em>Tf</em> alleles of NCRC and other <em>Carassius</em> populations were intermixed in five clades, two <em>Tf</em> alleles, together with <em>Cy</em>. <em>carpio</em>, formed a distinct monophyletic clade. Moreover, network and genetic structure analyses failed to distinguish between wild and cultured populations. Although AMOVA analysis based on <em>Tf</em> alleles indicated genetic differentiation among all six populations, the majority of the genetic variation (92.00 %) was observed within populations. Furthermore, the population dynamics analysis suggested that these populations have maintained relative stability in recent history. Therefore, the elevated nucleotide diversity of <em>Tf</em> alleles in cultured populations, the ambiguous population structure, and the limited genetic divergence among the six <em>Carassius</em> populations highlighted the hybrid genetic characteristics of HFJ and NCRC. These findings, based on <em>Tf</em> alleles, differ from the results obtained using mitochondrial gene markers, providing an alternative perspective for germplasm evaluation of hybrid varieties and crucian carp breeding.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 4","pages":"Pages 185-193"},"PeriodicalIF":0.0,"publicationDate":"2024-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266707122400036X/pdfft?md5=fbce2efe51ea9e707cd7bef7ae993539&pid=1-s2.0-S266707122400036X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141962809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Supplemental effect of wild strawberry (Fragaria vesca) pulp extract in extender to enhance quality attributes of cryopreserved bovine sperms","authors":"Khawaja Raees Ahmad ,&nbsp;Sadia Suleman ,&nbsp;Fiza Malik ,&nbsp;Muhammad Ali Kanwal ,&nbsp;Syeda Nadia Ahmad ,&nbsp;Sidra Yasmeen ,&nbsp;Iram Inayat ,&nbsp;Asma Younis ,&nbsp;Kausar Raees","doi":"10.1016/j.repbre.2024.06.004","DOIUrl":"https://doi.org/10.1016/j.repbre.2024.06.004","url":null,"abstract":"<div><p>The use of herbal bio-actives and phyto-contents to increase livestock productivity is a revived interest in veterinary research. The effect of wild strawberry {<em>Fragaria vesca</em> (FV)} pulp extract (PE) as antioxidant supplement in bovine semen cryopreservation diluent was explored. The experiment involved three fertile bulls. Three semen ejaculates per animal were collected. Each sample was diluted with Semen Transportation Diluent (STD) in 1:2 ratio. The (semen + STD) sample was vortexed and centrifuged (at 32g for 15 min) to remove 50 % supernatant. The remaining sample was re-diluted with semen cryopreservation diluent (SCD) in the ratio 1:4. Three aliquots of 0.3 ml were set aside from each (semen + STD + SCD) sample and were further treated with; 1: 0.7 mL of SCD (SCD group); 2: 0.7 mL SCD and 0.8 % FV-PE (FV-PE 0.8) and; 3: 0.7 mL SCD and 1.6 % FV-PE (FV-PE 1.6). Sperm quality parameters were evaluated before and after 48 h of cryopreservation. Results showed significantly enhanced mean percentage of sperm viability, progressive motility, velocity and fertilizability in FV-PE 0.8 and FV-PE 1.6 groups as compared with SCD group. The findings propose the protective and ameliorative potential of FV-PE for cryopreserved bovine sperms.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 3","pages":"Pages 179-184"},"PeriodicalIF":0.0,"publicationDate":"2024-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071224000280/pdfft?md5=ae3f93417370b47d671d10451ed1b9de&pid=1-s2.0-S2667071224000280-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141604952","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic parameter estimates for growth traits in Texel sheep of Brazil","authors":"Ali William Canaza-Cayo ,&nbsp;Fernando Amarilho Silveira ,&nbsp;Roxana Churata-Huacani ,&nbsp;Júlio Sílvio de Sousa Bueno Filho","doi":"10.1016/j.repbre.2024.07.001","DOIUrl":"https://doi.org/10.1016/j.repbre.2024.07.001","url":null,"abstract":"<div><p>The present study aimed to estimate the genetic parameters for direct and maternal influences on birth weight (BW), weaning weight (WW) and postweaning weight (PW) of Texel sheep, raised in an extensive system from Brazil. Data file used in this study consisted of 834 body weight records of 834 Texel lambs (376 males and 458 females) born from 2020 to 2021, offspring of 475 ewes and 45 rams. Multivariate animal model was conducted to estimate the variance components, heritability and genetic correlations using the restricted maximum likelihood method in ASReml-R software, while the effects of fixed factors were analyzed by the general linear model procedure of statistical analysis system. Five different animal models, with or without maternal genetic effect, maternal permanent environment effect and covariance between additive genetic and maternal genetic effects, were fitted on the growth traits data of the Texel sheep population. AIC and BIC test results were used to evaluate the best fit models for growth traits. The effect of lamb's sex was significant on BW, WW and PW while birth type and year of lambing effects were on all traits (P &lt; 0.05). Model 4, incorporating direct additive genetic, maternal additive genetic and maternal permanent environmental effects showed the minimum AIC estimate for all growth traits. The direct additive genetic heritability for BW, WW and PW were 0.11 ± 0.03, 0.37 ± 0.05 and 0.31 ± 0.08, respectively and maternal heritability for these traits were 0.00 ± 0.00, 0.04 ± 0.02 and 0.00 ± 0.00, respectively. The total heritability estimates for BW, WW and PW were 0.11 ± 0.03, 0.39 ± 0.05 and 0.31 ± 0.08, respectively. The estimates of the direct additive genetic correlation between BW-WW, BW-PW and WW-PW were 0.68 ± 0.12, 0.66 ± 0.12 and 0.78 ± 0.07, respectively. The maternal permanent environmental correlation between BW-WW, BW-PW and WW-PW were 0.38 ± 0.27, 0.29 ± 0.20 and 0.74 ± 0.16, respectively. Phenotypic correlation ranged from 0.43 ± 0.03 to 0.75 ± 0.02. It is concluded that due to the existence of genetic variation for BB, WW and PW and strong genetic correlation among them, genetic progress for these traits is possible by selection in Texel sheep from Brazil.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 3","pages":"Pages 174-178"},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071224000292/pdfft?md5=040d9bc869e3cb5ffc3ec953b1efefa0&pid=1-s2.0-S2667071224000292-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141582895","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative study of hypoxic tolerance between the hybrids of white crucian carp (♀) × red crucian carp (♂) and its parents","authors":"Kaikun Luo ,&nbsp;Yu Deng ,&nbsp;Lingmei Han ,&nbsp;Shengwei Wang,&nbsp;Yirui Zhang,&nbsp;Zhonghua Pen,&nbsp;Ting Liu,&nbsp;Chang Wang,&nbsp;Chengxi Liu,&nbsp;Min Tao,&nbsp;Chun Zhang,&nbsp;Rurong Zhao,&nbsp;Jing Wang","doi":"10.1016/j.repbre.2024.06.001","DOIUrl":"https://doi.org/10.1016/j.repbre.2024.06.001","url":null,"abstract":"<div><p>The hybrid of female white crucian carp (<em>Carassius auratus cuvieri</em>, WCC) × male red crucian carp (<em>Carassius auratus</em> red var. RCC) (WR), combines desirable characteristics of both parents, including tender meat, rapid growth rate, and strong resistance. To test the hypoxia tolerance of WR, an experiment of exposure to hypoxic conditions was conducted on WR, RCC, and WCC. The results showed that WR and RCC exhibited stronger hypoxia tolerance capacity compared to WCC. The strong resistance to hypoxia of WR and RCC was manifested by the absence of damage to the hepatopancreas after hypoxia, and the fish can adapt to the hypoxic environment by enhancing the hematopoietic function of hematopoietic tissue, remodeling gill morphology, and activating the antioxidant system. By contrast, after hypoxia, the number of red blood cells and the concentration of hemoglobin in the blood of WCC decreased, and the hepatopancreas was seriously damaged, indicating that the WCC is not as tolerant to low oxygen conditions as RCC and WR.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 3","pages":"Pages 164-173"},"PeriodicalIF":0.0,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071224000255/pdfft?md5=4cc5955441f42e33674f41b3a25eee31&pid=1-s2.0-S2667071224000255-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141582889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of dietary polyunsaturated fatty acid enriched diets on breeding, embryonic development and organogenesis of Rohu carp, Labeo rohita (Hamilton 1822)","authors":"Shahanaj Parvin Rumki ,&nbsp;Mohammad Moniruzzaman ,&nbsp;Md Saddam Hossain ,&nbsp;Tutul Kumar Saha ,&nbsp;Anika Tabassum ,&nbsp;Maria Binte Moin ,&nbsp;Shaon Kumar Mondol ,&nbsp;Sadia Ibnat ,&nbsp;Sungchul C. Bai ,&nbsp;Taesun Min ,&nbsp;Zakir Hossain","doi":"10.1016/j.repbre.2024.06.003","DOIUrl":"https://doi.org/10.1016/j.repbre.2024.06.003","url":null,"abstract":"<div><p>There is a considerable interest in supplementing fish diets with bio-functional substances such as polyunsaturated fatty acids (PUFAs), and research is being conducted to evaluate their effects on fish physiology, particularly on breeding performances. The objective of this study was to find out how squid-derived lipids (PUFAs) influence the Rohu carp, <em>Labeo rohita</em>, in terms of reproduction, and embryonic and larval growth. The experimental fish were provided with formulated diet for a duration of 90 days, containing 1 % squid-extracted lipids as a primary source of polyunsaturated fatty acids (PUFAs). To determine the accuracy of the formulation, a proximate analysis of the feed ingredients and the formulated feeds were conducted. The results demonstrated remarkable reproductive outcomes during the spawning trial. Observations in fish treated with PUFAs revealed a significant improvement (p &lt; 0.001) in sperm quality and viability, despite spawning occurring late in the breeding season. In addition, the fertilization rate (92.27 ± 1.74), hatching rate (89.33 ± 1.76), and survival rate (85.83 ± 1.74) of the offspring exhibited a statistically significant increase (<em>p</em> &lt; 0.05) when subjected to PUFAs treatment in <em>L. rohita</em>. Consequently, the treated fish exhibited enhanced early embryonic and larval development in comparison to the control group. Hence, the current study demonstrates that the inclusion of PUFAs in the diet of Rohu carp leads to improved maturation, breeding, and developmental outcomes, specifically in terms of embryonic development and organogenesis.</p></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"4 3","pages":"Pages 155-163"},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667071224000279/pdfft?md5=1570793242ee6daefecf3a28adada178&pid=1-s2.0-S2667071224000279-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141543715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}