Reproduction and breeding最新文献

{"title":"Captive breeding practices and insights on four indigenous Betta species of Sarawak","authors":"Ahmad Syafiq Ahmad Nasir ,&nbsp;Mohd Armeen Zulkanaini ,&nbsp;Jeffery Mahmud ,&nbsp;Ruhana Hassan ,&nbsp;Mohamad Faizul Mat Isa ,&nbsp;Samsur Mohamad","doi":"10.1016/j.repbre.2025.08.002","DOIUrl":"10.1016/j.repbre.2025.08.002","url":null,"abstract":"<div><div>Sarawak, located on the island of Borneo, is home to several indigenous <em>Betta</em> species, many of which face threats from habitat degradation. Despite their ecological and ornamental significance, limited information exists on their captive breeding potential. This study investigated the reproductive behavior and larval development of four native <em>Betta</em> species (<em>Betta brownorum</em>, <em>Betta ibanorum</em>, <em>Betta macrostoma</em>, and <em>Betta taeniata</em>) under controlled conditions. A total of 46 wild specimens were collected from four locations across Sarawak and subjected to species-specific breeding trials in aquaria. <em>B. brownorum</em>, a bubble-nester, exhibited nest-building and egg deposition behavior, with hatching observed within 3–4 days. In contrast, the three mouthbrooding species exhibited paternal incubation, which lasted 3–4 weeks. Larval development was monitored over time, showing steady increases in total length and distinct developmental stages were documented. These findings confirm the feasibility of captive breeding in all four species and highlight differences in reproductive strategy and larval growth patterns. The outcomes provide valuable baseline data for conservation planning and the development of ex-situ breeding programs for Sarawak's threatened <em>Betta</em> species.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 3","pages":"Pages 144-152"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144913240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comprehensive analysis of high-oil peanut cultivars in China: Agronomic performance, disease resistance, and breeding insights","authors":"Zhuo Li ,&nbsp;Yaru Zhang ,&nbsp;Yinghui Liu ,&nbsp;Yi Fan ,&nbsp;Ding Qiu ,&nbsp;Zhongfeng Li ,&nbsp;Yanzhong Zhou ,&nbsp;Fangping Gong ,&nbsp;Dongmei Yin","doi":"10.1016/j.repbre.2025.06.001","DOIUrl":"10.1016/j.repbre.2025.06.001","url":null,"abstract":"<div><div>Peanut (<em>Arachis hypogaea</em> L.) is a globally significant oilseed crop, with China being the largest producer and consumer. High-oil peanut varieties, characterized by oil content exceeding 55 %, offer substantial economic and nutritional benefits. This study comprehensively analyzed 238 high-oil peanut varieties in China, focusing on their agronomic traits, quality characteristics, yield potential, and disease resistance. Correlation analysis highlights a trade-off between oil and protein content, posing a challenge for breeding high-protein, high-oil varieties. Disease resistance analysis indicates that while a considerable proportion of varieties exhibit resistance to major diseases like leaf spot, bacterial wilt, and rust, the level of high resistance remains low, necessitating further breeding efforts. Pedigree analysis underscores the importance of key parents like Kaixuan 016 and CTWE in high-oil peanut breeding. The findings suggest integrating molecular breeding techniques, exploring wild relatives for genetic diversity, and developing multi-trait breeding objectives to enhance oil content, disease resistance, and environmental adaptability. This research provides a solid foundation for future breeding programs aimed at improving the yield, quality, and resilience of high-oil peanut varieties to meet the growing global demand for edible oils.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 3","pages":"Pages 102-109"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144279576","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genome-wide analysis of population structure and genetic diversity in two Ethiopian native sheep populations","authors":"Belayneh Engidawork Demissie ,&nbsp;Getinet Mekuriaw Tarekegn ,&nbsp;Hailu Dadi ,&nbsp;Zewdu Edea ,&nbsp;Adugna Abdi Woldesemayat ,&nbsp;Kwan-Suk Kim ,&nbsp;Selfu Girma ,&nbsp;Tamrayehu Seyoum ,&nbsp;Kalkidan Melaku ,&nbsp;Simenew Keskes","doi":"10.1016/j.repbre.2025.05.003","DOIUrl":"10.1016/j.repbre.2025.05.003","url":null,"abstract":"<div><div>Indigenous Ethiopian sheep inhabit diverse environmental conditions and hold distinct morphological traits, including variations in tail phenotypes. Characterizing their genetic diversity is crucial for developing appropriate breeding and conservation schemes. The current study aimed to determine the genetic diversity and population structure of two underrepresented indigenous sheep populations in Ethiopia: Selale and Semien. A total of 48 whole blood samples were collected randomly from the 24 Selale and 24 Semien sheep. Publicly available single nucleotide polymorphism genotype data from five Ethiopian sheep populations (Arsi-Bale, Menz, Blackhead Somali, Horro, and Adilo) were included in the analysis. Additionally, for an even broader perspective, four breeds from Asia and other parts of Africa (Afshari, Egyptian Barki, Red Maasai, and Indian Garole) were used to evaluate the genetic connections of the study populations. The average genetic diversity (expected heterozygosity) ranged from 0.325 for Ari-Bale to 0.362 for the Selale sheep, with a mean of (H_E = 0.344) across all populations. Estimates of genetic differentiation among sheep populations in Ethiopia ranged from 0.019 between Selale and Horro sheep to 0.077 between Semien and Arsi-Bale sheep. The analysis of molecular variance indicated that 94.25 % and 3.56 % of the genetic differentiation were attributable to variances within and among populations, respectively. Principal component and admixture analyses revealed three distinct genomic backgrounds of indigenous sheep populations in Ethiopia, consistent with their tail morphology and ecological distribution: long fat-tailed, short fat-tailed, and fat-rumped sheep. These findings can help formulate strategies for sustainable genetic improvement, conservation, and utilization of indigenous sheep populations.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 3","pages":"Pages 110-118"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144289158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A transcriptome-based analysis reveals the role of Hedgehog signaling in regulating slow muscle autophagy in Chinese perch","authors":"Yangyang Meng,&nbsp;Wei Zeng,&nbsp;Xin Liao,&nbsp;Lingtao Nie,&nbsp;Junzhao Wang,&nbsp;Xin Zhu,&nbsp;Jianshe Zhang,&nbsp;Wuying Chu","doi":"10.1016/j.repbre.2025.07.001","DOIUrl":"10.1016/j.repbre.2025.07.001","url":null,"abstract":"<div><div>As one of the structural tissues of fish skeletal muscles, slow muscle plays an important role in physiological and metabolic activities. The Hedgehog (Hh) signaling pathway has been reported to be involved in regulating the development and metabolism of slow muscle in fish. The underlying molecular mechanisms by which the Hedgehog (Hh) signaling pathway governs metabolic homeostasis within the slow muscle tissue of fish remain an enigma. In order to reveal the functional regulatory role of the Hh signaling pathway in the slow muscle of Chinese perch (<em>Siniperca chuatsi</em>), Cyclopamine was used to inhibit the Hh signaling pathway and Illumina sequencing technology was employed to explore the effect of inhibiting the Hh signaling pathway on slow muscle transcriptomes. In this study, a total of 666 differentially expressed genes (DEGs) were obtained between the control and Cyclopamine-treated groups. Functional enrichment analyses showed that the DEGs are mainly enriched in signaling pathways related to autophagy and metabolism, such as the mTOR signaling pathway, the autophagy pathway and the FoxO signaling pathway. The Western blotting analysis showed that the autophagy levels increased after treatment with Cyclopamine. Transmission electron microscopy results showed that the number of autophagic lysosomes was reduced in the Cyclopamine-treated group. The above results suggest that the Hh signaling pathway may play a key role in the regulation of autophagy and metabolism in slow muscle of Chinese perch, thus ensuring normal physiological activities in their muscles. In addition, the present study provides a direction for further elucidating the role of Hh signaling pathway in regulating the dynamic balance of metabolism in the slow muscle of Chinese perch.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 3","pages":"Pages 125-134"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144653032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving the growth performance of local pigs in Benin by crossbreeding with the stress-negative Pietrain","authors":"Constant Boris Bankolé ,&nbsp;Ignace Ogoudanan Dotché ,&nbsp;Serge Gbênagnon Ahounou ,&nbsp;Mahamadou Dahouda ,&nbsp;Issaka Youssao Abdou Karim ,&nbsp;Marcel Senou","doi":"10.1016/j.repbre.2025.05.004","DOIUrl":"10.1016/j.repbre.2025.05.004","url":null,"abstract":"<div><div>Local pigs in Benin are highly valued by the population, yet their suboptimal performance limits their breeding potential. The objective of this study was to enhance the zootechnical performance of local pigs in Benin by crossing them with the stress-negative Pietrain. A total of three batches were created. The first group consisted of 12 local females, mated by a local boar (LO), the second of 12 Pietrain females, inseminated with Pietrain semen (Pi), and the third of 12 local females inseminated with Pietrain semen (LO x Pi). The growth performance of piglets from each batch was collected from birth to 34 weeks of age. The data were subjected to a two-criteria analysis of variance, including the effects of piglet genetic type and sex. The results indicated that piglet weight, performance, and average daily gain (ADG) varied significantly (p &lt; 0.001) according to genetic type. From farrowing to 34 weeks of age, crossbred piglets (F1) had significantly higher weights than local piglets (P &lt; 0.05). The same was true of ADG. The Pietrain exhibited significantly higher growth performance than the other two genetic types. The observed performances did not vary according to sex within the same genetic type. In general, females were heavier than males between 28 and 34 weeks of age, regardless of genotype. The heterosis effect of the cross was positive, with a range of 3.71–11.14 %. The findings of this study indicate that the Pietrain genotype improves the growth performance of local pigs in Benin.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 3","pages":"Pages 95-101"},"PeriodicalIF":0.0,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144196100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The expression of liver–gonadal axis genes reveals the long–term effects of exogenous estradiol on gonad development and liver function in GIFT tilapia","authors":"Ye Yuan ,&nbsp;Zexun Zhou ,&nbsp;Shandong Chen ,&nbsp;Yunyun Liu ,&nbsp;Yongchun Li ,&nbsp;Yan Miao ,&nbsp;Shi Wang ,&nbsp;Zhongyuan Shen ,&nbsp;Lei Zeng ,&nbsp;Jun Xiao ,&nbsp;Qinbo Qin ,&nbsp;Wuhui Li","doi":"10.1016/j.repbre.2025.02.003","DOIUrl":"10.1016/j.repbre.2025.02.003","url":null,"abstract":"<div><div>The aim of this study was to investigate the effects of exogenous estradiol on the expression of the liver<strong>–</strong>gonadal axis gene in GIFT tilapia. Larval fish (5 dph) were fed a diet with 100 mg kg⁻¹ 17α–ethynylestradiol (EE2) for 30 days and then transferred to a conventional diet. Sex reversal statistics revealed that more than 60 % of the genotypic male fish transformed into pseudofemales. Histological results showed that the oocytes in pseudofemales developed asynchronously and had a prolonged maturation time compared to those in normal female fish. Global expression and DNA methylation analyses revealed that the expression patterns of the gonad and liver genes in pseudofemales were similar to those in XX female fish. Compared to XY male fish, the differentially expressed genes (DEGs) in pseudofemale gonads were enriched in FoxO signaling pathway, oocyte meiosis and other hormone–regulated pathways, which may affect the growth and development of the ovary. Moreover, the DEGs in liver were enriched mainly in protein digestion and absorption, steroid biosynthesis, and some disease and energy metabolism pathways, which may affect liver immunity and energy metabolism. Furthermore, the co-upregulated genes in the liver and gonads were enriched mainly in steroid biosynthesis and glycolysis/gluconeogenesis, whereas the co-downregulated genes were enriched in GnRH secretion and oxytocin signaling pathway. In addition, some genes associated with hormone secretion, growth, metabolism, and immunity in the liver‒gonad axis, such as <em>Oxt</em>, <em>Spoll</em> and <em>Piwi12,</em> were activated, whereas <em>Hox</em>, <em>cyp21a</em>, <em>gas1</em> and other genes were inhibited. These results indicated that EE2 affects gene expressions in liver and gonad tissues through the hypothalamic–pituitary–gonadal–liver (HPGL) axis.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 2","pages":"Pages 11-20"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143577185","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenotypic variability analysis of key sorghum (Sorghum bicolor (L) moench) genotypes under dry lowland areas","authors":"Temesgen Begna","doi":"10.1016/j.repbre.2025.03.007","DOIUrl":"10.1016/j.repbre.2025.03.007","url":null,"abstract":"<div><div>Sorghum is a climate-resilient food security and nutrition crop, which has been produced as a staple food in the semi-arid tropical regions of the world. However, moisture stress is increasingly affecting sorghum performance, especially at the flowering stage when water availability is critical for grain filling, thus reducing the sorghum grain yield. Therefore, the experiment was conducted to identify the best-adapted superior sorghum genotypes and quantify genetic variability, heritability and genetic advance as percent of mean for yield and yield components of sorghum genotypes. An alpha lattice experimental design with two replications was used to assess 42 sorghum genotypes in total at Mieso and Kobo. The combined analyses of variance revealed the presence of substantial genetic variation among sorghum genotypes for yield and agronomic traits. Among the traits with high genotypic coefficients of variation and heritability observed for plant height, panicle width and panicle yield were linked with higher values of genetic advance as a percentage of the mean. This result indicates that additive gene action governs the variability of these traits. High heritability and genetic advancement percentages were obtained for plant height (95.63 and 45.39 %, respectively), 1000-seed weight (77.98 and 23.86 %, respectively), panicle exertion (74.87 and 51.88 %, respectively), and panicle yield (65.43 and 37.32 %, respectively). The maximum grain yield was obtained from genotype 4x14 (6.32 tha^-1), followed by genotypes 8x15 (5.92 tha^-1), 1x15 (5.88 tha^-1), 13x14 (5.78 tha^-1) and 6x15 (5.57 tha^-1), with an average value of 5.00 tha^-1, which was greater than the mean value of the check (ESH-4) (4.77 tha^-1). In general, this study revealed wider genetic variability in the tested genotypes for different traits under moisture stress conditions. The selection and hybridization on these genotypes for a desired traits with high (H²) coupled with higher GCV and GAM will be effective to develop and select high yielding, stable and early sorghum genotypes. The results of this investigation would help determine the most pertinent genetic material and plan the subsequent breeding program to encourage efforts for varietal improvement. The most promising and potential genotypes that could be used commercially were identified by the study following an in-depth investigation of their superiority and yield stability over time across different parts of the country.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 2","pages":"Pages 79-87"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143816826","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Erratum regarding previously published articles","authors":"","doi":"10.1016/j.repbre.2025.03.003","DOIUrl":"10.1016/j.repbre.2025.03.003","url":null,"abstract":"","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 2","pages":"Pages 92-93"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143937550","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Next generation breeding in the omics era","authors":"Pasquale Tripodi","doi":"10.1016/j.repbre.2025.05.001","DOIUrl":"10.1016/j.repbre.2025.05.001","url":null,"abstract":"<div><div>The last two centuries have seen the occurrence of several theoretical and scientific advancements that revolutionized plant science. From Mendel's intuitions to discovery of DNA toward up to the development of next generation sequencing technologies, it is nowadays possible to precisely investigate the genetic basis of traits of agricultural interest accelerating crop improvement. The availability of catalogues of thousands of genomic markers in many species, provides new opportunities to identify favourable allelic variants supporting genomic assisted breeding programs. In addition, the release of complete plant genomes sequences and the development of pangenomes make possible the identification of structural variants facilitating mining of favourable alleles. Beyond technological advancements, data integration is crucial for the comprehensive understanding of the biological processes underlying plant traits and their interactions with the environment. This perspective article illustrates how omics technologies can be used to tailor genomic tools for different breeders' needs to revolutionize crop improvement.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 2","pages":"Pages 88-91"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143923320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Commercial fish genomics: A bibliometric and visualized study","authors":"Yan Wang ,&nbsp;Liang Guo ,&nbsp;Qifang Yu ,&nbsp;Jie Hu ,&nbsp;Yu Deng","doi":"10.1016/j.repbre.2025.04.001","DOIUrl":"10.1016/j.repbre.2025.04.001","url":null,"abstract":"<div><div>Bibliometric analysis is a scientific computer-assisted review methodology that can visualize the knowledge base and associated trends and patterns in a given topic or scientific field based on a quantitative review of the corresponding literature. This study aimed to explore the publication trends and growth potential of commercial fish genomics. The Web of Science Core Collection database was used to collect the literature associated with commercial fish genomics from the period between 2000 and 2023. The visualization analysis was then achieved by using Microsoft Excel 2019, VOSviewer and CiteSpace V. This study offers a fresh perspective and clear roadmap regarding global trends and hotspots in the field of commercial fish genomics, which could also lay a foundation for researchers to guide further research interests.</div></div>","PeriodicalId":74667,"journal":{"name":"Reproduction and breeding","volume":"5 2","pages":"Pages 69-78"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143807956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}