Pathologie (Heidelberg, Germany)最新文献_第7页

Mitteilungen der Deutschen Gesellschaft für Pathologie. Mitteilungen der Deutschen Gesellschaft für Pathologie.

Pathologie (Heidelberg, Germany) Pub Date : 2024-05-01 DOI: 10.1007/s00292-024-01323-8

引用次数: 0

[Xanthogranulomatous adrenalitis : A rare and difficult differential diagnosis of adrenal gland tumors]. [黄疽性肾上腺炎：肾上腺肿瘤罕见且难以鉴别诊断的疾病]。

Pathologie (Heidelberg, Germany) Pub Date : 2024-05-01 Epub Date: 2024-03-12 DOI: 10.1007/s00292-024-01312-x

Wolfgang Saeger, Andreas M Luebke, S T Mekoula, Jörg-Michael Pahnke

引用次数: 0

[Fluorescence confocal microscopy-complete digitization of pathology]. [荧光共聚焦显微镜--病理学完全数字化]。

Pathologie (Heidelberg, Germany) Pub Date : 2024-05-01 Epub Date: 2024-03-06 DOI: 10.1007/s00292-024-01311-y

Andreas G Loth, Anne Fassl, Felix K H Chun, Jens Köllermann, Sylvia Hartmann, Steffen Gretser, Paul K Ziegler, Nadine Flinner, Falko Schulze, Peter J Wild, Maximilian N Kinzler

{"title":"[Fluorescence confocal microscopy-complete digitization of pathology].","authors":"Andreas G Loth, Anne Fassl, Felix K H Chun, Jens Köllermann, Sylvia Hartmann, Steffen Gretser, Paul K Ziegler, Nadine Flinner, Falko Schulze, Peter J Wild, Maximilian N Kinzler","doi":"10.1007/s00292-024-01311-y","DOIUrl":"10.1007/s00292-024-01311-y","url":null,"abstract":"Background: Fluorescence-based confocal microscopy (FCM) can be used to create virtual H&E sections in real time. So far, FCM has been used in dermato-, uro-, and gynecopathology. FCM allows the creation of a completely digitized frozen section, which could potentially replace conventional frozen sections in the future.Objective: The aim of the current work is to implement FCM technology as a component of fully digitized processes in the pathological workflow. For this purpose, the current use of FCM in liver transplant pathology will be extended to other disciplines such as urology and otorhinolaryngology.Materials and methods: The FCM technique continues to be used prospectively on native tissue samples from potential donor livers. Conventional frozen sections are used comparatively to virtual FCM scans.Results: The data show a nearly perfect agreement for the detection of cholangitis, fibrosis, and malignancy, and a high level of agreement for, e.g., macrovesicular steatosis, inflammation, steatohepatitis, and necrosis between virtual FCM scans and conventional routine diagnostic frozen sections.Conclusion: Since the availability of time- and cost-intensive frozen section diagnostics in the context of transplant pathology in continuous operation (24/7) is now only established at very few university centers in Germany due to an increasing shortage of specialists, the use of FCM could be an important building block in the current process leading towards a fully digitized pathology workflow and should thus be extended to various disciplines.","PeriodicalId":74402,"journal":{"name":"Pathologie (Heidelberg, Germany)","volume":" ","pages":"211-217"},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11045597/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140041149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Adenoid cystic carcinoma of the ceruminous glands]. [耵聍腺样囊性癌]。

Pathologie (Heidelberg, Germany) Pub Date : 2024-05-01 Epub Date: 2024-02-19 DOI: 10.1007/s00292-024-01309-6

Stephanie Strobl, Matthias M Gaida

引用次数: 0

[Development and progress of the National Autopsy Network (NATON)]. [国家尸检网络（NATON）的发展与进步]。

Pathologie (Heidelberg, Germany) Pub Date : 2024-05-01 Epub Date: 2024-03-01 DOI: 10.1007/s00292-024-01307-8

Svenja Windeck, Kristina Allgoewer, Saskia von Stillfried, Lucas Triefenbach, Ulrike Nienaber, Roman David Bülow, Rainer Röhrig, Benjamin Ondruschka, Peter Boor

{"title":"[Development and progress of the National Autopsy Network (NATON)].","authors":"Svenja Windeck, Kristina Allgoewer, Saskia von Stillfried, Lucas Triefenbach, Ulrike Nienaber, Roman David Bülow, Rainer Röhrig, Benjamin Ondruschka, Peter Boor","doi":"10.1007/s00292-024-01307-8","DOIUrl":"10.1007/s00292-024-01307-8","url":null,"abstract":"Background: Autopsies have long been considered the gold standard for quality assurance in medicine, yet their significance in basic research has been relatively overlooked. The COVID-19 pandemic underscored the potential of autopsies in understanding pathophysiology, therapy, and disease management. In response, the German Registry for COVID-19 Autopsies (DeRegCOVID) was established in April 2020, followed by the DEFEAT PANDEMIcs consortium (2020-2021), which evolved into the National Autopsy Network (NATON).Deregcovid: DeRegCOVID collected and analyzed autopsy data from COVID-19 deceased in Germany over three years, serving as the largest national multicenter autopsy study. Results identified crucial factors in severe/fatal cases, such as pulmonary vascular thromboemboli and the intricate virus-immune interplay. DeRegCOVID served as a central hub for data analysis, research inquiries, and public communication, playing a vital role in informing policy changes and responding to health authorities.Naton: Initiated by the Network University Medicine (NUM), NATON emerged as a sustainable infrastructure for autopsy-based research. NATON aims to provide a data and method platform, fostering collaboration across pathology, neuropathology, and legal medicine. Its structure supports a swift feedback loop between research, patient care, and pandemic management.Conclusion: DeRegCOVID has significantly contributed to understanding COVID-19 pathophysiology, leading to the establishment of NATON. The National Autopsy Registry (NAREG), as its successor, embodies a modular and adaptable approach, aiming to enhance autopsy-based research collaboration nationally and, potentially, internationally.","PeriodicalId":74402,"journal":{"name":"Pathologie (Heidelberg, Germany)","volume":" ","pages":"203-210"},"PeriodicalIF":0.0,"publicationDate":"2024-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11045591/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139998432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Artificial intelligence for pathology-how, where, and why?] [病理学人工智能--如何、在哪里、为什么？］

Pathologie (Heidelberg, Germany) Pub Date : 2024-05-01 Epub Date: 2024-03-12 DOI: 10.1007/s00292-024-01314-9

Peter Schüffler, Katja Steiger, Carolin Mogler

引用次数: 0

[Effects of upstream laboratory processes on the digitization of histological slides]. [上游实验室流程对组织学切片数字化的影响]。

Pathologie (Heidelberg, Germany) Pub Date : 2024-03-01 Epub Date: 2024-02-22 DOI: 10.1007/s00292-024-01303-y

Leander Schwaibold, Sven Mattern, Markus Mählmann, Leon Lobert, Thomas Breunig, Christian M Schürch

{"title":"[Effects of upstream laboratory processes on the digitization of histological slides].","authors":"Leander Schwaibold, Sven Mattern, Markus Mählmann, Leon Lobert, Thomas Breunig, Christian M Schürch","doi":"10.1007/s00292-024-01303-y","DOIUrl":"10.1007/s00292-024-01303-y","url":null,"abstract":"Background: Several factors in glass slide (GS) preparation affect the quality and data volume of a digitized histological slide. In particular, reducing contamination and selecting the appropriate coverslip have the potential to significantly reduce scan time and data volume.Goals: To objectify observations from our institute's digitization process to determine the impact of laboratory processes on the quality of digital histology slides.Materials and methods: Experiment 1: Scanning the GS before and after installation of a central console in the microtomy area to reduce dirt and statistical analysis of the determined parameters. Experiment 2: Re-coverslipping the GS (post diagnostics) with glass and film. Scanning the GS and statistical analysis of the collected parameters.Conclusion: The targeted restructuring in the laboratory process leads to a reduction of GS contamination. This causes a significant reduction in the amount of data generated and scanning time required for the digitized sections. Film as a coverslip material minimizes processing errors in contrast to glass. According to our estimation, all the above-mentioned points lead to considerable cost savings.","PeriodicalId":74402,"journal":{"name":"Pathologie (Heidelberg, Germany)","volume":" ","pages":"90-97"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10901962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139934589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Histomolecular classification of urothelial carcinoma of the urinary bladder : From histological phenotype to genotype and back]. [膀胱尿路上皮癌的组织分子分类：从组织学表型到基因型再到组织学表型]。

Pathologie (Heidelberg, Germany) Pub Date : 2024-03-01 Epub Date: 2024-01-29 DOI: 10.1007/s00292-024-01305-w

Alexandra K Stoll, Florestan J Koll, Markus Eckstein, Henning Reis, Nadine Flinner, Peter J Wild, Jochen Triesch

{"title":"[Histomolecular classification of urothelial carcinoma of the urinary bladder : From histological phenotype to genotype and back].","authors":"Alexandra K Stoll, Florestan J Koll, Markus Eckstein, Henning Reis, Nadine Flinner, Peter J Wild, Jochen Triesch","doi":"10.1007/s00292-024-01305-w","DOIUrl":"10.1007/s00292-024-01305-w","url":null,"abstract":"Background: Of all urothelial carcinomas (UCs), 25% are muscle invasive and associated with a 5-year overall survival rate of 50%. Findings regarding the molecular classification of muscle-invasive urothelial carcinomas (MIUCs) have not yet found their way into clinical practice.Objectives: Prediction of molecular consensus subtypes in MIUCs with artificial intelligence (AI) based on histologic hematoxylin-eosin (HE) sections.Methods: Pathologic review and annotation of The Cancer Genome Atlas (TCGA) Bladder Cancer (BLCA) Cohort (N = 412) and the Dr. Senckenberg Institute of Pathology (SIP) BLCA Cohort (N = 181). An AI model for the prediction of molecular subtypes based on annotated histomorphology was trained.Results: For a five-fold cross-validation with TCGA cases (N = 274), an internal TCGA test set (N = 18) and an external SIP test set (N = 27), we reached mean area under the receiver operating characteristic curve (AUROC) scores of 0.73, 0.8 and 0.75 for the classification of the used molecular subtypes \"luminal\", \"basal/squamous\" and \"stroma-rich\". By training on correlations to individual molecular subtypes, rather than training on one subtype assignment per case, the AI prediction of subtypes could be significantly improved.Discussion: Follow-up studies with RNA extraction from various areas of AI-predicted molecular heterogeneity may improve molecular classifications and thereby AI algorithms trained on these classifications.","PeriodicalId":74402,"journal":{"name":"Pathologie (Heidelberg, Germany)","volume":" ","pages":"106-114"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10901926/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139572255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

[Explainable artificial intelligence in pathology]. [病理学中可解释的人工智能]。

Pathologie (Heidelberg, Germany) Pub Date : 2024-03-01 Epub Date: 2024-02-05 DOI: 10.1007/s00292-024-01308-7

Frederick Klauschen, Jonas Dippel, Philipp Keyl, Philipp Jurmeister, Michael Bockmayr, Andreas Mock, Oliver Buchstab, Maximilian Alber, Lukas Ruff, Grégoire Montavon, Klaus-Robert Müller

引用次数: 0

[Challenges of automation in quantitative evaluation of liver biopsies : Automatic quantification of liver steatosis]. [肝活检定量评估自动化面临的挑战：肝脏脂肪变性的自动量化]。

Pathologie (Heidelberg, Germany) Pub Date : 2024-03-01 Epub Date: 2024-02-21 DOI: 10.1007/s00292-024-01298-6

Jessica Darling, Nada Abedin, Paul K Ziegler, Steffen Gretser, Barbara Walczak, Ana Paula Barreiros, Falko Schulze, Henning Reis, Peter J Wild, Nadine Flinner

{"title":"[Challenges of automation in quantitative evaluation of liver biopsies : Automatic quantification of liver steatosis].","authors":"Jessica Darling, Nada Abedin, Paul K Ziegler, Steffen Gretser, Barbara Walczak, Ana Paula Barreiros, Falko Schulze, Henning Reis, Peter J Wild, Nadine Flinner","doi":"10.1007/s00292-024-01298-6","DOIUrl":"10.1007/s00292-024-01298-6","url":null,"abstract":"Background: Metabolic dysfunction-associated steatotic liver disease (MASLD), or non-alcoholic fatty liver disease (NAFLD), is a common disease that is diagnosed through manual evaluation of liver biopsies, an assessment that is subject to high interobserver variability (IBV). IBV can be reduced using automated methods.Objectives: Many existing computer-based methods do not accurately reflect what pathologists evaluate in practice. The goal is to demonstrate how these differences impact the prediction of hepatic steatosis. Additionally, IBV complicates algorithm validation.Materials and methods: Forty tissue sections were analyzed to detect steatosis, nuclei, and fibrosis. Data generated from automated image processing were used to predict steatosis grades. To investigate IBV, 18 liver biopsies were evaluated by multiple observers.Results: Area-based approaches yielded more strongly correlated results than nucleus-based methods (⌀ Spearman rho [ρ] = 0.92 vs. 0.79). The inclusion of information regarding tissue composition reduced the average absolute error for both area- and nucleus-based predictions by 0.5% and 2.2%, respectively. Our final area-based algorithm, incorporating tissue structure information, achieved a high accuracy (80%) and strong correlation (⌀ Spearman ρ = 0.94) with manual evaluation.Conclusion: The automatic and deterministic evaluation of steatosis can be improved by integrating information about tissue composition and can serve to reduce the influence of IBV.","PeriodicalId":74402,"journal":{"name":"Pathologie (Heidelberg, Germany)","volume":" ","pages":"115-123"},"PeriodicalIF":0.0,"publicationDate":"2024-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10901975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139914225","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0