Pathologie (Heidelberg, Germany)最新文献

{"title":"Reconstructing 3D histological structures using machine learning (artificial intelligence) algorithms.","authors":"J Báskay, M Kivovics, D Pénzes, E Kontsek, A Pesti, A Kiss, M Szócska, O Németh, P Pollner","doi":"10.1007/s00292-024-01387-6","DOIUrl":"10.1007/s00292-024-01387-6","url":null,"abstract":"<p><strong>Background: </strong>Histomorphometry is currently the gold standard for bone microarchitectural examinations. This relies on two-dimensional (2D) sections to deduce the spatial properties of structures. Micromorphometric parameters are calculated from these sections based on the assumption of a plate-like 3D microarchitecture, resulting in the loss of 3D structure due to the destructive nature of classical histological processing.</p><p><strong>Materials and methods: </strong>To overcome the limitation of histomorphometry and reconstruct the 3D architecture of bone core biopsy samples from 2D histological sections, bone core biopsy samples were decalcified and embedded in paraffin. Subsequently, 5 µm thick serial sections were stained with hematoxylin and eosin and scanned using a 3DHISTECH PANNORAMIC 1000 Digital Slide Scanner (3DHISTECH, Budapest, Hungary). A modified U‑Net architecture was trained to categorize tissues on the sections. LoFTR feature matching combined with affine transformations was employed to create the histologic reconstruction. Micromorphometric parameters were calculated using Bruker's CTAn software (v. 1.18.8.0, Bruker, Kontich, Belgium) for both histological and microCT datasets.</p><p><strong>Results: </strong>Our method achieved an overall accuracy of 95.26% (95% confidence interval (CI): [94.15%, 96.37%]) with an F‑score of 0.9320 (95% CI: [0.9211, 0.9429]) averaged across all classes. Correlation coefficients between micromorphometric parameters measured on microCT imaging and histological reconstruction showed a strong linear relationship, with Spearman's ρ‑values of 0.777, 0.717, 0.705, 0.666, and 0.687 for bone volume/tissue volume (BV/TV), bone surface/TV, trabecular pattern factor, trabecular thickness, and trabecular separation, respectively. Bland-Altman and mountain plots indicated good agreement between the methods for BV/TV measurements.</p><p><strong>Conclusion: </strong>This method enables examination of tissue microarchitecture in 3D with an even higher resolution than microcomputed tomography (microCT), without losing information on cellularity. However, the limitation of this procedure is its destructive nature, which precludes subsequent mechanical testing of the sample or any further secondary measurements. Furthermore, the number of histological sections that can be created from a single sample is limited.</p>","PeriodicalId":74402,"journal":{"name":"Pathologie (Heidelberg, Germany)","volume":" ","pages":"67-73"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142683707","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Why tumors of the urinary bladder?]","authors":"N T Gaisa, A Hartmann","doi":"10.1007/s00292-024-01355-0","DOIUrl":"https://doi.org/10.1007/s00292-024-01355-0","url":null,"abstract":"","PeriodicalId":74402,"journal":{"name":"Pathologie (Heidelberg, Germany)","volume":"45 6","pages":"361-362"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142514285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[New edition of the Paris classification 2022: What is new?]","authors":"Tatjana Vlajnic, Lukas Bubendorf","doi":"10.1007/s00292-024-01340-7","DOIUrl":"10.1007/s00292-024-01340-7","url":null,"abstract":"<p><p>As an internationally accepted diagnostic system, the Paris classification has achieved a global breakthrough in the standardization of diagnoses in urine cytology. Based on experience over the past few years since its first publication, the new edition of the Paris classification refines the diagnostic criteria and discusses diagnostic pitfalls. While the detection of high-grade urothelial carcinoma remains the main focus, other aspects of urine cytology, including cytology of the upper urinary tract and the associated challenges, have also been addressed. Low-grade urothelial neoplasia is no longer listed as a separate category but is now included in the category \"negative for high-grade urothelial carcinoma\" (NGHUC). Essentially, the Paris classification provides an important basis for estimating the risk of malignancy and further clinical management.</p>","PeriodicalId":74402,"journal":{"name":"Pathologie (Heidelberg, Germany)","volume":" ","pages":"389-396"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11511721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141494488","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Report of the Molecular Pathology Working Group of the German Society for Pathology].","authors":"N Pfarr, V Tischler, J Sperveslage","doi":"10.1007/s00292-024-01378-7","DOIUrl":"10.1007/s00292-024-01378-7","url":null,"abstract":"","PeriodicalId":74402,"journal":{"name":"Pathologie (Heidelberg, Germany)","volume":" ","pages":"114-115"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11695454/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634285","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Centers for experimental animal pathology still occupy a small but valuable niche in translational medicine-time for change?]","authors":"Tanja Poth, Peter Schirmacher","doi":"10.1007/s00292-024-01383-w","DOIUrl":"10.1007/s00292-024-01383-w","url":null,"abstract":"<p><p>Since CRISPR/Cas systems can be easily used to establish new genetically engineered mouse models, application of these models in the translational research field to explore predictive and therapeutic approaches for human diseases is rising.Integrative centers for experimental animal pathology, such as the CMCP in Heidelberg and the CEP in Munich, link the veterinary and human pathology discipline and contribute substantially to meaningful study results by combining the technical, research, and diagnostic expertise for phenotyping, evaluating, and interpretating complex animal models in the context of human pathology. They provide high-quality tissue processing in a broad spectrum of standard and specialized tissue-based technologies. The histopathology platform enables animal model evaluation including phenotyping, scoring, and detecting species-specific \"background lesions.\" By providing essential prerequisites for high-quality, reproducible, and sustainable research in translational medicine, facilities for experimental animal pathology optimally fulfill the requirements for publications in top-class professional journals, contribute to the implementation of the 3R principle for animal welfare, and help to reduce costs in the preclinical research.The importance of animal models reflecting human diseases will increase in the future but the requirements for high-quality tissue processing and comparative pathology/morphological phenotyping cannot be met by single facilities in a niche position. To cover the existing gap and expected rising demand in the German and European translational research landscape, further facilities for experimental animal pathology that are integrated in the human pathology environment should be established.</p>","PeriodicalId":74402,"journal":{"name":"Pathologie (Heidelberg, Germany)","volume":" ","pages":"31-36"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142649799","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[FGFR2/3 in urothelial carcinoma].","authors":"Michael Rose, Nadine T Gaisa","doi":"10.1007/s00292-024-01363-0","DOIUrl":"10.1007/s00292-024-01363-0","url":null,"abstract":"","PeriodicalId":74402,"journal":{"name":"Pathologie (Heidelberg, Germany)","volume":" ","pages":"409-411"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142302870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Pediatric soft tissue tumors : Tumors of uncertain origin].","authors":"K Greimelmaier, C Vokuhl","doi":"10.1007/s00292-024-01356-z","DOIUrl":"10.1007/s00292-024-01356-z","url":null,"abstract":"<p><p>Soft tissue tumors of childhood are an extremely heterogeneous group of tumors that require precise diagnosis for therapy. In this article, selected tumors of uncertain origin that exhibit characteristic histological, immunophenotypical, and molecular features are addressed. Angiomatoid fibrous histiocytoma, alveolar soft part sarcoma, extrarenal rhabdoid tumor, synovial sarcoma, and desmoplastic small round cell tumor differ in their pathology, their clinical behavior, and prognosis.</p>","PeriodicalId":74402,"journal":{"name":"Pathologie (Heidelberg, Germany)","volume":" ","pages":"412-421"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142362515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Report of the German Society for Pathology Gastroenteropathology Working Group].","authors":"Jens Neumann, Alexander Quaas","doi":"10.1007/s00292-024-01380-z","DOIUrl":"10.1007/s00292-024-01380-z","url":null,"abstract":"","PeriodicalId":74402,"journal":{"name":"Pathologie (Heidelberg, Germany)","volume":" ","pages":"95-96"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142570561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Reinhard Spanel : 4 March 1943-28 March 2024].","authors":"Jörg Placke","doi":"10.1007/s00292-024-01359-w","DOIUrl":"10.1007/s00292-024-01359-w","url":null,"abstract":"","PeriodicalId":74402,"journal":{"name":"Pathologie (Heidelberg, Germany)","volume":" ","pages":"125-126"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142333929","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"[Report of the German Society of Pathology Working Group on Pediatric and Fetal Pathology].","authors":"Elise Gradhand, Thomas Hager","doi":"10.1007/s00292-024-01358-x","DOIUrl":"10.1007/s00292-024-01358-x","url":null,"abstract":"","PeriodicalId":74402,"journal":{"name":"Pathologie (Heidelberg, Germany)","volume":" ","pages":"108-109"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11695383/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142333930","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}