Parasites, hosts and diseases最新文献_第3页

Exploration of Naegleria-preferentially secreted proteins for identifying diagnostic candidates to detect Naegleria fowleri. 探索纳格里氏杆菌优先分泌蛋白，以确定诊断候选物以检测福氏纳格里氏杆菌。

IF 1.3

Parasites, hosts and diseases Pub Date : 2026-01-01 Epub Date: 2026-01-26 DOI: 10.3347/PHD.25058

Hye-Jeong Jo, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon

{"title":"Exploration of Naegleria-preferentially secreted proteins for identifying diagnostic candidates to detect Naegleria fowleri.","authors":"Hye-Jeong Jo, Hae-Ahm Lee, Fu-Shi Quan, Hyun-Hee Kong, Eun-Kyung Moon","doi":"10.3347/PHD.25058","DOIUrl":"10.3347/PHD.25058","url":null,"abstract":"Naegleria fowleri is a free-living amoeba that can cause primary amebic meningoencephalitis (PAM), a very serious infection of the central nervous system. Early diagnosis of PAM is challenging, and the condition is almost always fatal. In this study, we conducted 2-dimensional gel electrophoresis (2-DE) analysis using N. fowleri trophozoite lysates and conditioned media to identify preferentially secreted proteins. As a result of the 2-dimensional gel electrophoresis analysis, 1 protein was found to increase, 5 proteins were found to decrease, 3 proteins showed a qualitative increase, and 15 proteins showed a qualitative decrease in the conditioned media compared to the proteins in the trophozoite lysates. Using cDNA from N. fowleri, Acanthamoeba castellanii, and Balamuthia mandrillaris, all of which can cause encephalitis, real-time PCR was performed on 5 genes corresponding to the p23-like domain-containing protein, cystatin-like domain-containing protein, fowlerpain-2, hemerythrin family non-heme iron protein, and an uncharacterized protein. The results showed that all 5 genes were highly expressed in N. fowleri. In animal models infected with N. fowleri resulting in PAM, real-time PCR analysis of brain tissue revealed significant overexpression of the p23-like domain-containing protein and fowlerpain-2. These results suggest that the 2 secreted proteins could provide valuable insights for developing antibody-based or molecular diagnostic methods to detect N. fowleri in patients with PAM.","PeriodicalId":74397,"journal":{"name":"Parasites, hosts and diseases","volume":" ","pages":"27-36"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12890559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146047380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A fatal case of complex hepatic alveolar echinococcosis. 复杂肝肺泡包虫病1例死亡。

IF 1.3

Parasites, hosts and diseases Pub Date : 2026-01-01 Epub Date: 2026-01-22 DOI: 10.3347/PHD.25022

Li Xin, Li Mengmeng, Yu Huixia, Zan Runna, Li Guojun, Shang Rongjian, Yu Jia

{"title":"A fatal case of complex hepatic alveolar echinococcosis.","authors":"Li Xin, Li Mengmeng, Yu Huixia, Zan Runna, Li Guojun, Shang Rongjian, Yu Jia","doi":"10.3347/PHD.25022","DOIUrl":"10.3347/PHD.25022","url":null,"abstract":"Hepatic alveolar echinococcosis (HAE), a life-threatening zoonosis, poses formidable surgical challenges when involving critical vasculature. Herein, we reported the periprocedural management dilemmas in radical resection for advanced HAE. A 58-year-old female visited the outpatient department presented with HAE. Imaging examination revealed extensive invasion of the hilum, bile duct, and several hepatic vessels, as well as left adrenal metastasis. The patient underwent right trisegmentectomy with left hepatic vein reconstruction, auto-transplantation, and adrenalectomy, with intraoperative Doppler demonstrating patent portal flow before abdominal closure. However, emergency thrombectomy and transcatheter thrombolysis were performed due to the abrupt occurrence of portal vein thrombosis 3 h after surgery. Despite intervention, the residual liver volume remained insufficient (approximately 28% of the standard liver volume), leading to progressive liver failure. The patient expired from multiorgan failure 9 days after operation. This case underscores not only the critical balance between radical resection and preservation of residual liver function in the surgical management of complex HAE, but also the imperative need to establish a comprehensive postoperative thromboprophylaxis.","PeriodicalId":74397,"journal":{"name":"Parasites, hosts and diseases","volume":"64 1","pages":"98-103"},"PeriodicalIF":1.3,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12890552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146159558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vaccinia virus expressing MIC8 and AMA1 provides protection against Toxoplasma gondii ME49 infection. 表达MIC8和AMA1的牛痘病毒对弓形虫ME49感染具有保护作用。

IF 1.3

Parasites, hosts and diseases Pub Date : 2025-11-01 Epub Date: 2025-11-19 DOI: 10.3347/PHD.25054

Hae-Ji Kang, Yan Jin, Zhao-Shou Yang, Md Atique Ahmed, Fu-Shi Quan

{"title":"Vaccinia virus expressing MIC8 and AMA1 provides protection against Toxoplasma gondii ME49 infection.","authors":"Hae-Ji Kang, Yan Jin, Zhao-Shou Yang, Md Atique Ahmed, Fu-Shi Quan","doi":"10.3347/PHD.25054","DOIUrl":"10.3347/PHD.25054","url":null,"abstract":"Toxoplasma gondii is an intracellular protozoan parasite capable of causing chronic infection by forming persistent cysts in the brain. Despite its global burden, no approved vaccine exists. Virus-like particle vaccines expressing microneme protein 8 (MIC8) or apical membrane antigen 1 (AMA1) of T. gondii have previously shown efficacy. In this study, we generated recombinant vaccinia viruses (rVVs) expressing MIC8 and AMA1 and evaluated their efficacy against T. gondii ME49 infection. BALB/c mice were intramuscularly immunized with a combination of MIC8 and AMA1 rVVs and challenged orally with T. gondii ME49. Immunization with MIC8+AMA1 rVVs produced a significant increase in T. gondii-specific IgG. Splenocyte analysis revealed robust activation of CD4+ and CD8+ T cells, as well as expansion of memory B cells. The immunized group exhibited an 89.6% reduction in brain cyst count, with significantly improved survival compared to the control group. These findings demonstrate that combining the antigens MIC8 and AMA1 using a vaccinia virus platform can effectively promote both humoral and cellular immunity, supporting its potential as a vaccine strategy against T. gondii ME49.","PeriodicalId":74397,"journal":{"name":"Parasites, hosts and diseases","volume":"63 4","pages":"340-348"},"PeriodicalIF":1.3,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12661178/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145643657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Complete mitogenome sequence of Caryophyllaeus brachycollis (Cestoda: Caryophyllidae) from China: Characterization and phylogenetic analyses of Caryophyllidea. 完成mitogenome序列Caryophyllaeus brachycollis(绦虫类:石竹亚纲)从中国:Caryophyllidea的描述和系统发育分析。

IF 1.3

Parasites, hosts and diseases Pub Date : 2025-11-01 Epub Date: 2025-11-19 DOI: 10.3347/PHD.25044

Yi-Liu Liu, Ya Zhang, Yi-Tian Fu, Guo-Hua Liu, Hui-Mei Wang, Yuan-Ping Deng

{"title":"Complete mitogenome sequence of Caryophyllaeus brachycollis (Cestoda: Caryophyllidae) from China: Characterization and phylogenetic analyses of Caryophyllidea.","authors":"Yi-Liu Liu, Ya Zhang, Yi-Tian Fu, Guo-Hua Liu, Hui-Mei Wang, Yuan-Ping Deng","doi":"10.3347/PHD.25044","DOIUrl":"10.3347/PHD.25044","url":null,"abstract":"Caryophyllaeus brachycollis mainly parasitizes the intestines of globally distributed freshwater fishes, and infection causes significant economic losses to the aquaculture industry. However, data on the molecular epidemiology, population genetics, and systematics of C. brachycollis are scarce. In this study, we sequenced the complete mitogenome of C. brachycollis isolated from Beijing, China. This circular mitogenome comprised 14,273 bp, which was 231 bp shorter than that of C. brachycollis isolated from Wuhan, China. The mitogenome contained 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and 2 noncoding regions. Bayesian inference revealed that C. brachycollis belonged to the family Caryophyllaeidae. The taxonomic status of C. brachycollis is controversial when based solely on morphological features. A comparative analysis of the mitogenome sequence obtained in this study revealed novel molecular markers for the accurate ascertainment of the phylogenetic position of this parasite.","PeriodicalId":74397,"journal":{"name":"Parasites, hosts and diseases","volume":"63 4","pages":"317-326"},"PeriodicalIF":1.3,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12661179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145643327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Detection of intestinal parasites in leopard cat fecal samples using shotgun metagenomics. 猎枪宏基因组法检测豹猫粪便肠道寄生虫。

IF 1.3

Parasites, hosts and diseases Pub Date : 2025-11-01 Epub Date: 2025-11-19 DOI: 10.3347/PHD.25032

Jun Ho Choi, Singeun Oh, Myung-Hee Yi, Dongjun Kang, Du-Yeol Choi, Xavier Chavarria, Arwa Shatta, Yoon Hee Cho, Seongjun Choe, Seung-Hun Lee, Ju Yeong Kim

引用次数: 0

Reconstruction of the nearly complete mitochondrial genome of a Danish isolate of Hymenolepis diminuta (Cyclophyllidea: Hymenolepididae) with extension of the noncoding region. 丹麦小膜膜绦虫（Cyclophyllidea: Hymenolepis hymenolepidia）近完整线粒体基因组的重建及非编码区扩展。

IF 1.3

Parasites, hosts and diseases Pub Date : 2025-11-01 Epub Date: 2025-11-19 DOI: 10.3347/PHD.25061

Kwangjae Cho, Minkyung Kim, Yang-Kyu Choi, Won Gi Yoo

{"title":"Reconstruction of the nearly complete mitochondrial genome of a Danish isolate of Hymenolepis diminuta (Cyclophyllidea: Hymenolepididae) with extension of the noncoding region.","authors":"Kwangjae Cho, Minkyung Kim, Yang-Kyu Choi, Won Gi Yoo","doi":"10.3347/PHD.25061","DOIUrl":"10.3347/PHD.25061","url":null,"abstract":"We present a nearly complete mitochondrial genome (mitogenome) of Hymenolepis diminuta from a Danish isolate, which was reassembled and comprehensively annotated using whole-genome sequencing data retrieved from the Sequence Read Archive (accession No. ERS056110). Although 2 mitogenomes of H. diminuta have previously been submitted to GenBank (accession No. AP017664, Danish isolate; NC_002767, putative German laboratory strain), the former lacks noncoding regions, while the latter harbors relatively short repeat units. Our newly reconstructed mitogenome is 14,090 bp long, comprising 12 protein-coding genes, 22 transfer RNA genes, 2 ribosomal RNA genes, and a 643-bp noncoding region containing 7 tandem repeat units. The annotated sequence has been deposited in the Third Party Annotation database in GenBank (accession No. BK071817). Phylogenetic analysis based on mitogenomic sequences confirmed a close relationship between H. diminuta and Hymenolepis nana. This improved mitogenome sequence represents a valuable resource for comparative mitogenomic and phylogenetic investigations within the families Hymenolepididae, Taeniidae, and Diphyllobothriidae.","PeriodicalId":74397,"journal":{"name":"Parasites, hosts and diseases","volume":"63 4","pages":"364-370"},"PeriodicalIF":1.3,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12661174/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145643609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Catatropis indicus (Digenea: Notocotylidae) from ducks in Indonesia with a brief review of the genus Catatropis. 印度尼西亚鸭属褐刺鸭属研究述评。

IF 1.3

Parasites, hosts and diseases Pub Date : 2025-11-01 Epub Date: 2025-11-19 DOI: 10.3347/PHD.25045

Jong-Yil Chai, Bong-Kwang Jung, Taehee Chang, Sooji Hong, Hyejoo Shin, Marzuki Bin Muhammad Abdullah

{"title":"Catatropis indicus (Digenea: Notocotylidae) from ducks in Indonesia with a brief review of the genus Catatropis.","authors":"Jong-Yil Chai, Bong-Kwang Jung, Taehee Chang, Sooji Hong, Hyejoo Shin, Marzuki Bin Muhammad Abdullah","doi":"10.3347/PHD.25045","DOIUrl":"10.3347/PHD.25045","url":null,"abstract":"Adult notocotylid flukes (Digenea: Notocotylidae) were recovered from the ceca of Pitalah ducks (Anas sp.) in Aceh Province, Indonesia. These flukes were morphologically characterized by a median ventral ridge with 2 lateral rows of ventral papillae and the absence of both a ventral sucker and pharynx, consistent with the characteristics of the genus Catatropis. They exhibited a genital pore located just posterior to the oral sucker, 10-11 pairs of ventral papillae, a deep and multi-lobed ovary and testes, a metraterm equal in length to the cirrus sac, and ceca bearing numerous diverticula; accordingly, they were identified as Catatropis indicus Srivastava, 1935. Adult specimens measured 3.01-3.77 mm (average 3.47 mm) in length and 0.98-1.21 mm (average 1.11 mm) in width (n=8). Uterine eggs measured 0.016-0.023 mm (average 0.019 mm) in length and 0.008-0.014 mm (average 0.012 mm) in width (n=20), each bearing 2 long polar filaments. These specimens resembled Catatropis vietnamensis Izrailskaia et al., 2019, and Catatropis pakistanensis Schuster and Wibbelt, 2012, sharing features such as a genital pore immediately posterior to the oral sucker and other morphological traits. However, they differed from C. vietnamensis by having a larger body, ceca with numerous diverticula, and a broader anterior distribution of vitelline follicles. They also differed from C. pakistanensis in possessing a longer esophagus and ceca with multiple diverticula. In 28S rDNA sequence analysis, our specimens showed 99.1% identity with both C. indicus and C. vietnamensis. In contrast, internal transcribed spacer 2 (ITS2) sequence comparisons revealed only 96.0%-96.1% identity with C. vietnamensis (no GenBank data available for C. indicus), suggesting that our specimens are phylogenetically distant from C. vietnamensis. This represents the first report of C. indicus from ducks in Indonesia. A brief review of Catatropis species is provided.","PeriodicalId":74397,"journal":{"name":"Parasites, hosts and diseases","volume":"63 4","pages":"303-316"},"PeriodicalIF":1.3,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12661176/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145643312","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Re: Impact of self-efficacy-based health education programs on behavior modification for Opisthorchis viverrini and cholangiocarcinoma prevention in Thailand: A systematic review and meta-analysis. 以自我效能感为基础的健康教育项目对泰国虎腹蛇行为矫正和胆管癌预防的影响：一项系统综述和荟萃分析。

IF 1.3

Parasites, hosts and diseases Pub Date : 2025-11-01 Epub Date: 2025-11-19 DOI: 10.3347/PHD.25037

Nathkapach Kaewpitoon Rattanapitoon, Chutharat Thanchonnang, Schawanya Kaewpitoon Rattanapitoon

引用次数: 0

Partial characterization of a cysteine protease inhibitor of Plasmodium vivax. 间日疟原虫半胱氨酸蛋白酶抑制剂的部分鉴定。

IF 1.3

Parasites, hosts and diseases Pub Date : 2025-11-01 Epub Date: 2025-11-19 DOI: 10.3347/PHD.25043

Tuấn Cường Võ, Jung-Mi Kang, Hương Giang Lê, Byoung-Kuk Na

{"title":"Partial characterization of a cysteine protease inhibitor of Plasmodium vivax.","authors":"Tuấn Cường Võ, Jung-Mi Kang, Hương Giang Lê, Byoung-Kuk Na","doi":"10.3347/PHD.25043","DOIUrl":"10.3347/PHD.25043","url":null,"abstract":"Cysteine proteases play key roles in the biology of Plasmodium parasites and are recognized as antimalarial drug targets. Because these enzymes are involved in diverse biological functions, precise regulation is required to prevent unnecessary damage to both parasites and hosts. In this study, we identified an endogenous inhibitor of cysteine protease of Plasmodium vivax (PvICP) and characterized its biochemical properties. PvICP was found to share highly similar structural characteristics with orthologous proteins from other Plasmodium species. Recombinant PvICP (rPvICP) expressed in Escherichia coli showed a broad range of inhibitory activity against falcipain family cysteine proteases, including vivapain-3, vivapain-4, falcipain-3, malapain-2, and malapain-4, with more potent inhibitory activity against vivapain-3 and vivapain-4. rPvICP's inhibitory activity was not significantly affected by pH, suggesting its broad biological functions. These findings provide new insights into PvICP and lay the groundwork for future studies exploring its biological significance and potential as a therapeutic target in malaria research.","PeriodicalId":74397,"journal":{"name":"Parasites, hosts and diseases","volume":"63 4","pages":"354-359"},"PeriodicalIF":1.3,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12661172/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145643501","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prevalence of gastrointestinal parasites in alpacas: A preliminary 1-year longitudinal study on a farm in Jeju, Korea. 羊驼胃肠道寄生虫的流行：在韩国济州一个农场进行的为期1年的初步纵向研究。

IF 1.3

Parasites, hosts and diseases Pub Date : 2025-11-01 Epub Date: 2025-11-19 DOI: 10.3347/PHD.25042

Seung-Ho Ryu, Eliot Forbes, Heon Woo Lee, Emmnuel Opara, Seongjun Choe

{"title":"Prevalence of gastrointestinal parasites in alpacas: A preliminary 1-year longitudinal study on a farm in Jeju, Korea.","authors":"Seung-Ho Ryu, Eliot Forbes, Heon Woo Lee, Emmnuel Opara, Seongjun Choe","doi":"10.3347/PHD.25042","DOIUrl":"10.3347/PHD.25042","url":null,"abstract":"Alpacas (Vicugna pacos), native to South America, were recently introduced to Korea, primarily for tourism-related activities. However, information on their parasitic infections in alpacas within the region are limited. This study aimed to investigate the longitudinal prevalence and diversity of gastrointestinal parasites in an alpaca herd on a single farm in Korea and to assess the potential risks for reinfection and interspecies transmission. Between June 2022 and June 2023 (excluding December 2022 and January 2023), fecal samples were collected monthly from a herd of 61 alpacas housed on a farm on Jeju Island, Korea. Coprological examination of 406 samples was performed using a modified McMaster technique, and parasites were identified morphologically. Six gastrointestinal parasite taxa were identified: strongyles, Trichuris sp., capillarids, Moniezia sp., Eimeria lamae, and Eimeria macusaniensis. The overall gastrointestinal parasite prevalence was 11.3%, with strongyles and Trichuris sp. most frequently identified. No seasonal trends were observed, and mixed infections were present in five animals. The prevalence was markedly lower compared with reports from Japan, Poland, and Australia, reflecting the restricted pasture access implemented by Korea's alpaca management systems, which are primarily tourism-oriented. Neither Nematodirus spp. nor zoonotic parasites were detected. This is the first longitudinal investigation of gastrointestinal parasites in alpacas in Korea. Although the overall prevalence was low, environmental contamination and the risk of reinfection are ongoing concerns. These findings highlight the need for routine surveillance, targeted parasite control, and consistent veterinary oversight, particularly as alpaca populations expand in Korea.","PeriodicalId":74397,"journal":{"name":"Parasites, hosts and diseases","volume":"63 4","pages":"293-302"},"PeriodicalIF":1.3,"publicationDate":"2025-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12661173/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145643538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0