American journal of cancer research最新文献

{"title":"Integration of serological markers with O-RADS ultrasound risk stratification achieves high diagnostic accuracy for early ovarian cancer: development and validation of an interpretable model.","authors":"Tingting Hua, Xinchun Wu, Weihui Liu, Zhiying Jia, Lianhua Zhang, Dilibaier Abuduniyazi, Yan Ma, Li Li, Xiaoyan Zhu, Muzaipaer Muhetaer, Abudushalamu Abulaiti, Fucheng Ma","doi":"10.62347/PGIS8026","DOIUrl":"https://doi.org/10.62347/PGIS8026","url":null,"abstract":"<p><p>This study aimed to enhance preoperative diagnostic accuracy for early ovarian cancer (EOC) by constructing and validating an interpretable predictive model integrating serological markers with O-RADS ultrasound risk stratification. A total of 674 patients with adnexal masses (270 EOC, 404 benign) were enrolled and randomly assigned to training (n=438) and validation (n=236) sets. Multivariate logistic regression identified cancer antigen 125 (CA125; P=0.002), human epididymal protein 4 (HE4; P=0.001), carcinoembryonic antigen (CEA; P=0.005), maximum tumor diameter (P=0.005), and Ovarian-Adnexal Reporting and Data System (O-RADS) classification (P<0.001) as independent predictors. Among individual indices, O-RADS performed best (AUC=0.854), followed by CA125 (AUC=0.783), HE4 (AUC=0.744), CEA (AUC=0.717), and maximum tumor diameter (MTD; AUC=0.696). The comprehensive model achieved an AUC of 0.969 (95% CI: 0.953-0.985) in the training set and 0.973 (95% CI: 0.952-0.994) in the validation set, with sensitivity of 0.907/0.897, specificity of 0.914/0.980, and accuracy of 0.911/0.949, significantly outperforming both the marker combination model (AUC=0.918) and individual indices (DeLong test, all P<0.05). Robust calibration was confirmed (training set: Brier score=0.062, Hosmer-Lemeshow P=0.126; validation set: Brier score=0.056, Hosmer-Lemeshow P=0.578), and decision curve analysis demonstrated positive net benefits across a wide threshold probability range. SHAP analysis identified O-RADS classification, CA125, and HE4 as the most influential predictors, with notable interactions among them. Bootstrap resampling and 5-fold cross-validation confirmed model stability. The comprehensive model demonstrated excellent diagnostic performance, robust calibration, and clinical utility, providing a reliable interpretable tool for preoperative EOC risk stratification.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"16 7","pages":"2740-2762"},"PeriodicalIF":3.1,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13468248/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148759137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of perioperative hospital stay, postoperative chest tube retention, pain levels between uniportal and multiportal thoracoscopic lung cancer resection patients.","authors":"Yong Gao, Zhanqiang Zhai, Lichao Huang, Youcai Zhu, Huafei Chen, Xiaofeng Li, Puen Chen, Yonghua Min, Gang Lan","doi":"10.62347/QEPX4181","DOIUrl":"https://doi.org/10.62347/QEPX4181","url":null,"abstract":"<p><strong>Purpose: </strong>To compare perioperative hospital stay, chest tube retention time, and postoperative pain intensity between uniportal video-assisted thoracoscopic surgery (UVATS) and multiportal VATS (MVATS) for anatomical lung resection in patients with non-small cell lung cancer (NSCLC).</p><p><strong>Methods: </strong>This retrospective cohort study enrolled 201 patients (83 in the UVATS group and 118 in the MVATS group) who underwent pulmonary lobectomy or segmentectomy. Daily postoperative chest drainage volume was recorded within the first three postoperative days. Postoperative pain intensity was assessed via the Visual Analog Scale (VAS) at 24 hours, 48 hours and 7 days after surgery. Opioid consumption, converted to morphine milligram equivalents (MME), was documented within the initial 48 postoperative hours. Chest tube retention time, postoperative length of stay and surgical complications were compared between groups.</p><p><strong>Results: </strong>The UVATS group yielded significantly lower daily drainage volumes on postoperative day (POD) 1 (284.56 vs. 338.92 mL, P = 0.007), POD 2 (197.34 vs. 236.48 mL, P = 0.009) and POD 3 (130.58 vs. 159.12 mL, P = 0.004), as well as lower cumulative 72-hour drainage volume (612.48 vs. 743.52 mL, P = 0.001). Patients in the UVATS group reported remarkably lower VAS scores at 24 h (3.52 vs. 5.08, P<0.001), 48 h (2.98 vs. 4.22, P<0.001) and postoperative day 7 (2.21 vs. 2.75, P = 0.002), alongside reduced 48-hour opioid consumption (28.46 vs. 42.73 mg, P<0.001). Moreover, the UVATS group had shorter chest tube indwelling time (3.47 vs. 4.85 days, P<0.001) and shorter postoperative hospitalization (5.63 vs. 6.76 days, P = 0.001). The overall complication rate was also markedly lower in the UVATS group (14.46% vs. 27.12%, P = 0.033).</p><p><strong>Conclusion: </strong>Compared with MVATS, UVATS for NSCLC anatomical resection is associated with decreased postoperative pleural drainage, alleviated surgical pain, shortened chest tube indwelling and hospitalization duration, as well as reduced perioperative complications.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"16 7","pages":"3173-3182"},"PeriodicalIF":3.1,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13468236/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148756947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Raman microspectroscopy for cervical cancer diagnosis: an ex-vivo cryopreserved tissue analysis.","authors":"Mariana V Tavares, Maria M Félix, Ana Lm Batista de Carvalho, Inês P Santos, Maria F Botelho, Luís Ae Batista de Carvalho, Maria Pm Marques","doi":"10.62347/KCBL4300","DOIUrl":"https://doi.org/10.62347/KCBL4300","url":null,"abstract":"<p><p>Cervical cancer was the fourth most common cancer worldwide in 2022. There is an urgent clinical need for new objective and reproductible methods aiming an early diagnosis. The aim of the present study is to characterize fresh cryopreserved human cervical tissue through Raman microspectroscopy to identify spectral biomarkers that highlight the chemical differences between healthy and malignant tissues. Normal and tumoral human cervical tissues samples were collected prospectively from patients and from pathology specimens and cryopreserved at -80°C without any treatment or cryoprotectant. Two parallel 10 µm sections were cut from each tissue block for histopathology and spectroscopy analysis. Raman microspectroscopy using a 532 nm laser was performed in the first minutes after defrosting the sample. Concerning the high-wavenumber region (2800-3600 cm^-1) the signals from lipids and proteins are those that most contribute to the spectra of the tumoral samples. In the fingerprint region (600-1800 cm^-1) the spectra of the malignant samples show characteristic nucleic acids signals and absence of features from either collagen or glycogen, which are usually present in healthy tissues. Distinct vibrational spectroscopic differences were obtained, and these signals may be considered as spectral biomarkers of tumoral tissues. A successful classification model was constructed to categorize the samples into either healthy or tumoral, with an accuracy higher than 90%. This report evidences the feasibility of Raman microspectroscopy as a reliable and non-invasive diagnostic tool for differentiating healthy from tumoral tissues with high accuracy. The use of snap-frozen tissue samples allows the translation to real-time in vivo fresh samples. Coupled to existing histopathological methods, this technique can is therefore a useful tool in the clinical field for early cancer diagnosis.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"16 7","pages":"2873-2884"},"PeriodicalIF":3.1,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13468258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757180","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"SPP1 promotes the metastasis of liver cancer cells through modulation of the MT1L/vimentin axis.","authors":"Jen Yu, Pei-Huan Ho, Chau-Ting Yeh, Ming-Wei Lai, Yang-Hsiang Lin","doi":"10.62347/LALC6308","DOIUrl":"https://doi.org/10.62347/LALC6308","url":null,"abstract":"<p><p>Tumor metastasis, a hallmark of cancer progression including hepatocellular carcinoma (HCC), is closely linked to poor prognosis. Therefore, strategies to suppress tumor metastasis remain an unresolved challenge for improving patient care. We identified that the oncogenic gene secreted phosphoprotein 1 (SPP1) is highly expressed in HCC tissues. A pseudogene-derived long non-coding RNA, metallothionein 1L (MT1L), was found to be negatively regulated by SPP1. Clinically, MT1L expression was reduced in HCC specimens, and its higher expression correlated positively with favorable prognosis. Notably, MT1L expression was inversely correlated with SPP1 expression in HCC. Mechanistically, SPP1 suppressed MT1L expression through the integrin signaling pathway. Overexpression of MT1L inhibited cell motility and lipid accumulation by repressing vimentin expression. Furthermore, rescue experiments demonstrated that MT1L was functionally involved in SPP1-mediated effects. Collectively, our findings reveal a novel association among the SPP1/MT1L/integrin/vimentin axis in HCC progression, suggesting that targeting this pathway may represent a potential therapeutic approach for HCC.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"16 7","pages":"2985-2996"},"PeriodicalIF":3.1,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13468239/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148756958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"LncRNA MEG3 inhibits glioma progression by inducing pyroptosis through the miR-223-3p/FOXO1/PARP1 axis.","authors":"Xiaogang Yang, Xin Ai, Hongxing Sun, Wei Shi","doi":"10.62347/AQKT8060","DOIUrl":"https://doi.org/10.62347/AQKT8060","url":null,"abstract":"<p><p>Glioma, particularly glioblastoma (GBM), is a highly aggressive malignancy with limited therapeutic options. Pyroptosis, a pro-inflammatory regulated cell death, represents a promising tumor-suppressive mechanism; however, its precise regulatory networks in glioma remain incompletely defined. Although lncRNA MEG3 is a recognized tumor suppressor, whether it dictates glioma pyroptosis through a competing endogenous RNA (ceRNA) mechanism has yet to be characterized. In the study, we measured the expression profiles of MEG3, miR-223-3p, FOXO1 and PARP1 in glioma tissues and cultured cell lines. In vitro, we evaluated the biological effects of MEG3 modulation. Subcutaneous xenograft tumor models were established to investigate tumor progression in vivo. Dual-luciferase reporter and RIP assays validated the molecular interactions, and rescue experiments confirmed the functional importance of this axis. In comparison to normal counterparts, glioma specimens and cell models displayed a prominent reduction in MEG3 expression. Crucially, restoring MEG3 levels thwarted aggressive cellular traits (including proliferation, migration, and invasion) by prompting pyroptosis, a process validated by the elevated abundance of mature IL-1β, cleaved caspase-1, and GSDMD-N. Mechanistically, as a ceRNA molecule, MEG3 exerts its regulatory role by sponging and functionally neutralizing miR-223-3p, thus derepressing FOXO1 and PARP1 post-transcriptionally. Knockdown of FOXO1 or PARP1 effectively abrogated the pro-pyroptotic and anti-tumor effects induced by MEG3 overexpression. Consistently, MEG3 upregulation hindered tumor growth and triggered pyroptosis in vivo. In conclusion, our findings unveil a novel regulatory MEG3/miR-223-3p/FOXO1-PARP1 axis that drives pyroptosis to restrain glioma progression, which identifies this axis as a potential therapeutic target with translational value for glioma therapy.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"16 7","pages":"2971-2984"},"PeriodicalIF":3.1,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13468249/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148760595","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of oliceridine-based patient-controlled intravenous administration on postoperative pain and hemodynamic stability in elderly patients undergoing laparoscopic surgery for colorectal cancer.","authors":"Ling Ding, Xiaojing Ren, Shenyue Pang, Tao Yang, Shuhua Xie","doi":"10.62347/EPTA3201","DOIUrl":"https://doi.org/10.62347/EPTA3201","url":null,"abstract":"<p><p>The dose-effect relationship and hemodynamic effects of oliceridine in elderly patients undergoing laparoscopic surgery remain unclear. This study evaluated the effects of different doses of oliceridine administered via patient-controlled intravenous analgesia (PCIA) on postoperative pain management in older adults with colorectal cancer. Patients were divided into three groups according to the analgesic regimen: a control group (Group C, fentanyl PCIA), a low-dose oliceridine group (Group O1), and a high-dose oliceridine group (Group O2), each comprising 50 patients. Outcomes included resting and movement numeric rating scale (NRS) scores, incidence of moderate-to-severe pain, Ramsay sedation scores, Quality of Recovery-15 (QoR-15) scores, patient satisfaction, inflammatory cytokine levels, hemodynamic and respiratory parameters, and adverse reactions. The total opioid load was similar across the three groups. Group O2 showed consistently lower resting and movement NRS scores at all observation time points (all <i>P</i><0.001). It also had the lowest frequency of moderate-to-severe pain within the first 48 hours after surgery (<i>P</i><0.05). In addition, patients in the O2 group achieved earlier first ambulation and had a shorter length of hospital stay than the other groups (<i>P</i><0.05). Group O2 also showed a higher QoR-15 score, indicating better postoperative recovery quality (<i>P</i><0.001), along with higher analgesic satisfaction (<i>P</i>=0.001). Inflammatory cytokine levels decreased dose-dependently (<i>P</i><0.001). Hemodynamic parameters were more stable, and respiratory rate and saturation (SpO₂) improved more significantly in Group O2 (<i>P</i><0.05). The incidence of adverse reactions was comparable among the three groups of people (<i>P</i>>0.05). The number of remedial analgesia administrations within 24 hours postoperatively (0.4±0.2 times) and total flurbiprofen axetil consumption (25.0±12.5 mg) were significantly lower in Group O2 than in Groups O1 and C (<i>P</i><0.05). High-dose Oliceridine PCIA provided better analgesic effects for elderly Laparoscopic Colorectal Cancer Patients after surgery, effectively reduced inflammatory response, and maintained stable Hemodynamic and Respiratory Function.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"16 7","pages":"2885-2898"},"PeriodicalIF":3.1,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13468246/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757660","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the relationship between immunological microenvironment and prognosis in pancreatic cancer.","authors":"Shuji Suzuki, Yuichi Nagakawa, Mitsugi Shimoda, Ryoichi Miyamoto, Masahiro Shiihara, Hiroaki Osakabe, Moe Matsumoto, Hiroshi Yamaguchi, Toshitaka Nagao","doi":"10.62347/WYLC8512","DOIUrl":"https://doi.org/10.62347/WYLC8512","url":null,"abstract":"<p><p>Immune cell infiltration in tumors can serve as a prognostic marker in many cancers; however, its role in pancreatic ductal adenocarcinoma (PDAC) remains understudied. In this retrospective study, we aimed to evaluate the prognostic significance of the immunological microenvironment and immune cell infiltration in PDAC, considering both preoperative clinicophysiological and postoperative clinicopathological factors. Furthermore, we evaluated the same analyses after excluding patients who had received neoadjuvant treatment for the subgroup analysis. We included 189 patients who underwent radical resection for pancreatic cancer between October 2007 and October 2015 at our center and an affiliated university hospital. Immunohistochemical staining for clusters of differentiation (CD3, CD8, CD19, and CD20) was performed on the largest cross-sectional area of the tumor specimens to quantify immune cell infiltration. Disease-free survival (DFS) and overall survival (OS) were analyzed for each antigen. Preoperative clinicophysiological and postoperative clinicopathological factors were compared based on positively labeled cell counts. Among the 189 patients, 114 were men (average age, 69.3 years). CD20- and CD8-positive cell counts were significantly associated with DFS (<i>P</i>=0.027) and OS (<i>P</i>=0.033), respectively. No significant differences were observed between groups in terms of T factor for CD8, tumor location, biliary decompression, albumin level, C-reactive protein level, hemoglobin level, N factor, or disease stage for CD20. Multivariate analyses identified disease stage (<i>P</i>=0.04) and CD20 expression (<i>P</i>=0.043) as independent predictors of DFS, while CD8 expression (<i>P</i>=0.016) was the only independent predictor of OS. These results indicate that high CD20 expression, reflecting B-cell infiltration, predicts DFS, whereas high CD8 expression, reflecting T-cell infiltration, predicts OS. Collectively, our findings suggest that the immunological microenvironment represents a potential prognostic factor in patients with PDAC.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"16 7","pages":"2861-2872"},"PeriodicalIF":3.1,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13468254/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perioperative symptom burden as an independent predictor of long-term functional and survival outcomes in oral squamous cell carcinoma patients undergoing free flap reconstruction: a retrospective cohort study.","authors":"Dandan Li, Qingli He, Haixin Zhang","doi":"10.62347/BSSW4014","DOIUrl":"https://doi.org/10.62347/BSSW4014","url":null,"abstract":"<p><p>This study aimed to evaluate the prognostic significance of acute perioperative symptom burden as an independent predictor for long-term functional recovery and survival outcomes in patients with oral squamous cell carcinoma (OSCC) undergoing microvascular free flap reconstruction. A retrospective cohort of 355 OSCC patients treated between June 2022 and December 2024 at Henan Provincial People's Hospital was analyzed. All patients underwent primary tumor resection followed by immediate microvascular free flap reconstruction. Perioperative symptom burden was quantified using the validated MD Anderson Symptom Inventory-Head and Neck module (MDASI-HN). Based on the median core severity score on postoperative day 3 (POD 3) of 5.79, patients were stratified into low symptom burden (n=178) and high symptom burden (n=177) groups. Over a 12-month follow-up period, comprehensive assessments encompassed long-term speech function measured by the Speech Handicap Index (SHI) and Percent Consonants Correct (PCI), swallowing function evaluated via the MD Anderson Dysphagia Inventory (MDADI), quality of life assessed by the University of Washington Quality of Life questionnaire (UW-QOL) and the Functional Assessment of Cancer Therapy-Head and Neck (FACT-H&N), as well as survival outcomes. Multivariate linear regression, Cox proportional hazards models, and subgroup analyses were employed to ascertain the independent prognostic value of symptom burden. Findings revealed that elevated perioperative symptom burden was significantly associated with delayed and incomplete functional recovery. At 12 months, the high symptom burden cohort demonstrated markedly worse SHI scores (29.2 vs. 19.2, t=12.45, P<0.001), reduced PCI (84.4% vs. 94.4%, t=-15.32, P<0.001), and lower MDADI scores (84.0 vs. 94.0, t=-14.88, P<0.001). Multivariate analysis identified the area under the curve (AUC) of the perioperative MDASI severity trajectory as an independent predictor for 12-month SHI (B=2.83, 95% CI 1.52-4.14, P=0.001) and UW-QOL scores (B=-3.82, 95% CI -5.14 to -2.50, P=0.001). Furthermore, a high symptom burden was independently correlated with poorer overall survival (Log-rank P=0.019) and recurrence-free survival (Log-rank P=0.025); Cox regression confirmed high symptom burden as an independent risk factor for overall survival (HR=1.78, 95% CI 1.06-2.99, P=0.029). Subgroup analyses upheld the robustness of these associations across diverse clinical and demographic strata. In conclusion, acute perioperative symptom burden transcends being a mere transient reflection of surgical trauma, serving instead as a pivotal independent prognosticator of long-term functional outcomes, quality of life, and survival in OSCC patients undergoing free flap reconstruction. Early identification and proactive management of perioperative symptoms may constitute a novel therapeutic avenue to enhance both oncologic and functional prognoses.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"16 7","pages":"2919-2936"},"PeriodicalIF":3.1,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13468264/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Clinical value of serum osteocalcin (N-MID) combined with thyroid transcription factor-1 for predicting bone metastasis of lung cancer.","authors":"Yang Li, Bingxiang Wu, Wenjun Zhou","doi":"10.62347/TSGZ3774","DOIUrl":"https://doi.org/10.62347/TSGZ3774","url":null,"abstract":"<p><p>This study aimed to evaluate the clinical predictive value of combining N-terminal serum osteocalcin (N-MID) with thyroid transcription factor-1 (TTF-1) for bone metastasis in lung cancer. This retrospective analysis included 147 lung cancer patients admitted to the hospital between February 2020 and October 2024. Patients were divided into a bone metastasis group (n=83) and a non-bone metastasis group (n=64) based on the presence of bone metastases. Baseline clinical data and laboratory parameters, including TTF-1, N-MID, tumor abnormal protein (TAP), parathyroid hormone (PTH), vascular endothelial growth factor (VEGF), total N-terminal propeptide of type I collagen (tPINP), and β-C-terminal telopeptide of type I collagen (β-CTX) were collected. Univariate and multivariate logistic regression analyses were performed to identify independent risk factors for bone metastasis. Model performance was evaluated using receiver operating characteristic (ROC) curves, integrated discrimination improvement (IDI), category-based net reclassification improvement (cNRI), the Hosmer-Lemeshow goodness-of-fit test, and decision curve analysis (DCA). Age-stratified analysis and external validation were further conducted to ensure the generalizability of study. Univariate analysis showed that TTF-1 positivity rate and the levels of TAP, PTH, VEGF, tPINP, β-CTX, and N-MID were significantly higher in the bone metastasis group than in the non-bone metastasis group (all <i>P</i><0.05). Multivariate logistic regression confirmed that TTF-1 (OR=2.217, 95% CI: 1.329-3.698, P<0.001) and N-MID (OR=2.519, 95% CI: 1.404-4.518, <i>P</i><0.001) were independent risk factors for bone metastasis in lung cancer. After adding TTF-1 and N-MID to a baseline model that included PTH, VEGF, tPINP, and β-CTX, the area under the curve (AUC) increased from 0.752 to 0.837, with an IDI of 0.079 and a cNRI of 0.609 (both P<0.001). The Hosmer-Lemeshow test indicated good model fit, and external validation demonstrated good concordance between predicted probabilities and observed outcomes. Age-stratified analysis revealed that in elderly populations (70-79 years and 80-89 years), the combined detection of TTF-1 and N-MID yielded significantly higher AUC than either marker alone. Decision curve analysis confirmed that the combined detection provided a higher net benefit within clinically reasonable threshold ranges. In conclusion, TTF-1 and N-MID are independent risk factors for bone metastasis in lung cancer, and their combination significantly enhances the diagnostic performance of the predictive model, with particularly notable clinical value in elderly patients. These findings provide evidence-based references for early identification of bone metastasis and individualized clinical intervention in lung cancer.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"16 7","pages":"3150-3161"},"PeriodicalIF":3.1,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13468252/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correlation of tumor-infiltrating lymphocyte spatial distribution patterns with HER2 expression status and prognostic significance in gastric adenocarcinoma: a retrospective study based on digital pathology.","authors":"Xinwu Mao, Shangwei Zhong, Xiaowen Zhang, Yuting Liu, Luqing Zhou","doi":"10.62347/PEJP1288","DOIUrl":"https://doi.org/10.62347/PEJP1288","url":null,"abstract":"<p><strong>Background: </strong>The spatial distribution of tumor-infiltrating lymphocytes (TILs) has prognostic value in solid tumors, but its relationship with HER2 status in gastric adenocarcinoma remains poorly characterized.</p><p><strong>Methods: </strong>TIL densities in the tumor center (CT) and at the invasive margin (IM) were assessed on H&E-stained slides using QuPath, and the edge/center ratio (E/C ratio = IM-TILs/CT-TILs) was calculated. The optimal cut-off for E/C ratio was determined by maximizing the log-rank statistic. Survival analyses were performed using Kaplan-Meier and Cox regression.</p><p><strong>Results: </strong>The HER2-positive group (n = 35) exhibited significantly lower CT-TILs (11.92% vs. 20.98%, P < 0.001) and a higher E/C ratio (2.11 vs. 1.25, P < 0.001) than the HER2-negative group (n = 165). Using an optimal cut-off of 1.57, high E/C ratio was associated with significantly worse overall survival (OS) in the whole cohort (median 14.64 vs. 42.87 months; log-rank P < 0.001). Multivariate Cox regression, adjusting for clinicopathological factors, revealed that the E/C ratio as a continuous variable was independently prognostic (HR = 1.398, P < 0.001), and a significant interaction between HER2 status and E/C ratio was observed (HR = 1.844, P = 0.001). Dichotomization at E/C > 1.57 provides significant prognostic stratification, an effect that is modified by HER2 status; however, due to the small HER2-positive subgroup, these findings require validation in larger independent cohorts.</p><p><strong>Conclusions: </strong>HER2-positive gastric adenocarcinoma exhibits an \"edge-limiting\" immune phenotype with elevated E/C ratio. Digital assessment of TILs on routine H&E sections is simple and promising for clinical translation.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"16 7","pages":"2937-2954"},"PeriodicalIF":3.1,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13468245/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}