American journal of cancer research最新文献

{"title":"NUCKS1 exacerbates hepatocellular carcinoma cell proliferation and metastasis via the upregulation of Cdc42.","authors":"Weiwang Fan, Yuchen Ma, Jiaming Wu, Xinchen Zhang","doi":"10.62347/IAMC6442","DOIUrl":"https://doi.org/10.62347/IAMC6442","url":null,"abstract":"<p><p>Nuclear casein kinase and cyclin-dependent kinases substrate 1 (NUCKS1) is overexpressed in hepatocellular carcinoma (HCC), but its role and regulatory mechanism in the development and progression of HCC remains unknown. Here, we report that the RNA and protein levels of NUCKS1 were significantly increased in HCC tissues. The inhibition of NUCKS1 notably decreased the proliferation and migration of SNU449 and HepG2 cells. However, NUCKS1 overexpression exacerbated cell growth and migration. Additionally, NUCKS1 depletion reduced the sphere formation efficiency and inhibited tumorigenesis in vivo. Mechanistically, depletion of NUCKS1 downregulated the expression of cell division control protein 42 (Cdc42), and NUCKS1 directly bound to the promoter of Cdc42 and transcriptionally upregulated Cdc42, which promoted the development and progression of HCC. Furthermore, the expression of NUCKS1 was positively associated with Cdc42 in HCC tissues. Collectively, our data indicate that the increasing expression of NUCKS1 plays an oncogenic role and promotes progression via transactivation of Cdc42 expression in HCC.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 3","pages":"1051-1065"},"PeriodicalIF":3.6,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and influencing factors of immunotherapy crossover combined with targeted therapy in advanced esophageal cancer patients following first-line chemotherapy combined with immunotherapy failure.","authors":"Xiuxiu Li, Fan Fan, Ti Zhang","doi":"10.62347/GBOQ6704","DOIUrl":"https://doi.org/10.62347/GBOQ6704","url":null,"abstract":"<p><strong>Background: </strong>Advanced esophageal cancer presents significant treatment challenges, especially after immunochemotherapy failure. This study evaluates the efficacy of further treatment with combination chemotherapy versus combination immunotherapy crossover in terms of tumor regression, quality of life, and identifies factors influencing treatment outcomes.</p><p><strong>Methods: </strong>In a retrospective case-control study, clinical data from 293 patients with advanced esophageal cancer treated at Shanxi Province Cancer Hospital between February 2021 and February 2023 were analyzed. Patients excluded from radical resection due to failure of first-line immunotherapy were divided into two groups: 95 received combination chemotherapy with Irinotecan and Tigio (S-1, Tegafur/Gimeracil/Oteracil Potassium), and 198 underwent Anlotinib targeted therapy combined with immunotherapy crossover. Treatment efficacy was assessed using tumor regression grading (TRG), and quality of life was evaluated using EORTC QLQ-C30 and QLQ-OES18 scales. Potential factors affecting treatment efficacy were examined using multivariate logistic regression analysis.</p><p><strong>Results: </strong>Baseline characteristics, including age, gender, body mass index (BMI), and history of smoking and alcohol consumption, were comparable between the two groups. TRG showed no significant differences in distribution, with objective response rates of 40% in the Irinotecan/S-1 group and 44.44% in the combined immunotherapy crossover group (P = 0.472). However, quality of life measures indicated superior outcomes from immunotherapy crossover in physical (P = 0.024), emotional (P = 0.002), and general health scores (P = 0.003). Factors negatively impacting treatment success included male gender, smoking, alcohol consumption history, and certain tumor locations. Elevated CEA levels positively correlated with treatment efficacy. Logistic regression analysis identified male gender (OR, 2.109; P = 0.021), smoking (OR, 2.575; P = 0.003), alcohol consumption (OR, 1.995; P = 0.043), and CEA levels (OR, 0.742; P = 0.017) as significant predictors of treatment efficacy.</p><p><strong>Conclusion: </strong>Immunotherapy combined with targeted therapy and chemotherapy alone showed comparable efficacy in tumor regression. However, immunotherapy combined with targeted therapy improved certain aspects of quality of life. Factors such as gender, lifestyle habits, and CEA levels can significantly influence treatment outcomes.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 3","pages":"1321-1334"},"PeriodicalIF":3.6,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958412","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Enhanced diagnostic accuracy of contrast-enhanced ultrasound in liver space-occupying lesions: superior sensitivity and specificity over conventional ultrasound.","authors":"Junfang Wang, Weiwei Shi, Yuanyuan Jiang","doi":"10.62347/BBUT6997","DOIUrl":"https://doi.org/10.62347/BBUT6997","url":null,"abstract":"<p><strong>Aims: </strong>To evaluate the clinical value of contrast-enhanced ultrasound (CEUS) in diagnosing liver space-occupying lesions.</p><p><strong>Methods: </strong>A total of 487 patients with liver space-occupying lesions were examined using both conventional ultrasound and CEUS. The diagnostic results from the two methods were compared, with pathological findings used as the gold standard. Sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), and the area under the receiver operating characteristic curve were calculated for each method to assess their diagnostic value.</p><p><strong>Results: </strong>Among the 487 lesions, 220 were malignant and 267 were benign. The relative blood flow (rBF) in the arterial phase of malignant lesions was significantly higher than that of benign lesions, while the rBF in the delayed phase was significantly lower (P<0.05). In diagnosing malignant lesions, CEUS had a higher detection rate than conventional ultrasound (75% vs. 43.18%, P<0.001). CEUS also demonstrated a higher diagnostic agreement for lesions ≤1 cm compared to conventional ultrasound (85.16% vs. 49.47%, P<0.001). The accuracy, sensitivity, specificity, PPV, and NPV of CEUS were all higher than those of conventional ultrasound (all P<0.05).</p><p><strong>Conclusion: </strong>CEUS is effective in diagnosing liver space-occupying lesions, with superior sensitivity and specificity compared to conventional ultrasound.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 3","pages":"1201-1212"},"PeriodicalIF":3.6,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982715/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143959145","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficacy and safety of endovascular repair versus open surgery for ruptured abdominal aortic aneurysm: a comparative study.","authors":"Mingkui Huang, Yinhe Tang","doi":"10.62347/NYJT8307","DOIUrl":"https://doi.org/10.62347/NYJT8307","url":null,"abstract":"<p><strong>Objective: </strong>Ruptured abdominal aortic aneurysm (rAAA) is a life-threatening condition with high mortality. This study compared the efficacy and safety of open surgical repair (OSR) and endovascular aortic repair (EVAR) in the treatment of rAAA.</p><p><strong>Methods: </strong>A retrospective analysis of clinical data was conducted for 232 rAAA patients treated at Taizhou Central Hospital and the First Affiliated Hospital of Wenzhou Medical College. Patients were divided into two groups based on surgical methods: OSR group (n=84) and EVAR group (n=148). Perioperative indicators, perioperative complication rates, and 1-year mortality rates were compared. Patients were further divided into a survival group (n=160) and a death group (n=72) based on their 1-year survival status, and the risk factors affecting the prognosis of rAAA patients were analyzed. Postoperative pain was evaluated using the Visual Analog Scale (VAS), Verbal Rating Scale (VRS), and Present Pain Intensity (PPI). Serum levels of C-reactive protein (CRP) and white blood cells (WBC), pro-inflammatory interleukins (IL-1α, IL-6, IL-8), and tumor necrosis factor-α (TNF-α) were measured before and after treatment using enzyme-linked immunosorbent assays (ELISA).</p><p><strong>Results: </strong>Compared with the OSR group, the EVAR group had significantly shorter surgical time, less intraoperative bleeding (IOB) and intraoperative blood transfusion volume, reduced intraoperative infusion volume, shorter fasting and first walk time, and shorter ICU and hospital days. The incidence of complications in the EVAR group was significantly lower than that in the OSR group (<i>P</i><0.05). Pain scores (VAS, VRS, and PPI) and serum levels of CRP, WBC, IL-1α, IL-6, IL-8, and TNF-α were significantly lower in the EVAR group than those in the OSR group (all <i>P</i><0.05). There was no significant difference in perioperative mortality between the two groups (28.95% vs. 11.80%, <i>P</i>>0.05). However, the 1-year mortality rate was significantly lower in the EVAR group (38.1% vs. 27.0%, P<0.05). Multivariate logistic regression analysis identified Alb<40 g/L (<i>P</i>=0.004), Cre≥1.5 mg/dL (<i>P</i>=0.007), urea nitrogen ≥25 mg/dL (<i>P</i>=0.001), ALT≥40 U/L (<i>P</i>=0.002), and treatment method (OSR) (<i>P</i>=0.024) as independent risk factors for poor postoperative prognosis.</p><p><strong>Conclusion: </strong>EVAR demonstrates significant advantages over OSR in reducing surgical trauma, decreasing postoperative complications, alleviating pain and inflammatory responses, and improving postoperative survival rates.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 3","pages":"1234-1247"},"PeriodicalIF":3.6,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982736/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Salidroside inhibits the invasion and migration of colorectal cancer cells by regulating MMP-12 and WNT signaling pathway.","authors":"Ye Hong, Yanrong Li, Xia Liu, Jia Deng, Yanli He, Bin Zhao","doi":"10.62347/KELA7583","DOIUrl":"https://doi.org/10.62347/KELA7583","url":null,"abstract":"<p><p>Colorectal cancer (CRC) is a prevalent and highly lethal malignancy, with current therapeutic efficacy limited by the tumor's high invasiveness and metastatic potential. Matrix metalloproteinases (MMPs) and the WNT (Wingless/Integrated) signaling pathway play key roles in the invasion and metastasis of CRC. Salidroside, a natural compound, has demonstrated inhibitory effects in several cancers, but its precise molecular mechanism in CRC cells remains unclear. This study aims to investigate the antitumor effect of salidroside on CRC and its molecular mechanism in influencing epithelial-mesenchymal transition (EMT) by regulating MMP-12 and the WNT signaling pathway. The effects of salidroside on CRC cell proliferation, migration, and invasion were evaluated through in vitro experiments using HCT-116 and SW620 cell lines. The antitumor effects of salidroside were validated using CCK-8, wound healing, and Transwell assays. Expression changes of MMP-12, WNT signaling-related proteins (e.g., β-catenin, GSK-3β), and EMT markers (e.g., E-cadherin, Vimentin) after salidroside treatment were measured by qRT-PCR and Western Blot. Additionally, bioinformatics analysis was performed using TCGA and GEO databases in combination with the BEST online tool to identify differentially expressed genes, followed by GSEA enrichment analysis. Salidroside showed significant antiproliferative and inhibitory effects on the migration and invasion of CRC cells. In vitro experiments demonstrated that salidroside significantly inhibited CRC cell proliferation and reduced their migration and invasion capabilities. qRT-PCR and Western Blot analyses showed that salidroside significantly downregulated MMP-12 expression and led to changes in the expression of WNT signaling and EMT-related proteins, specifically downregulating β-catenin, upregulating E-cadherin, and downregulating Vimentin. Furthermore, bioinformatics analysis indicated that MMP-12 plays a crucial role in salidroside-mediated CRC inhibition, further supporting its potential as a key target. In conclusion, salidroside suppresses CRC invasion and migration by downregulating MMP-12 and modulating the WNT signaling pathway, thereby inhibiting the EMT process. These findings suggest that salidroside holds potential as a therapeutic agent for CRC, offering a novel approach to CRC treatment.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 3","pages":"929-945"},"PeriodicalIF":3.6,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960659","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced EZH1/2 expression in imipridone-treated cells correlates with synergy following combinations with EZH1/2 or HDAC inhibitors in diffuse glioma and other tumors.","authors":"Yiqun Zhang, Kelsey E Huntington, Attila A Seyhan, Nikos Tapinos, Rishi R Lulla, Michelle Monje, Eric T Wong, Clark C Chen, Wafik S El-Deiry","doi":"10.62347/DZAT5333","DOIUrl":"https://doi.org/10.62347/DZAT5333","url":null,"abstract":"<p><p>Small molecule imipridones including ONC201, ONC206 and ONC212 have anti-cancer activity mediated in part through the integrated stress response, induction of TRAIL and its receptor DR5, and activation of mitochondrial caseinolytic protease ClpP with impaired oxidative phosphorylation. ONC201 provides clinical benefit in a subset of patients with histone H3K27M-mutated diffuse glioma (DG). We hypothesized that EZH2 inhibitors (EZH2i) may sensitize tumors to imipridones by mimicking H3K27M mutation. EZH1 is a homolog and alternative for EZH2 in assembling PRC2 complex. We combined ONC201, ONC206 or ONC212 plus dual EZH1/2i in tumors and observed synergy. We observed synergies with imipridones combined with HDACi or triple combination of ONC201/ONC206, EZH2i and HDACi in DG, GBM, prostate cancer and SCLC cells. Our observations implicate EZH1/2 suppression in mechanism of anti-cancer effect of imipridones. We investigated effects of imipridones on EZH1/2 in DG cells and solid tumor cells including GBM, CRC, PDAC, SCLC, prostate cancer, gastric cancer, HCC and breast cancer cells and found inhibition of EZH1/EZH2 expression across tumor types and cell viability suppression by imipridones is correlated with EZH1/2 reduction. Imipridone or EZH2i-treated tumor cells showed similar cytokine profile changes. RNA-seq showed ONC201 and EHZ2i tazemetostat-treated cells have similar transcriptional profiles and share overlap of top regulated genes. Thus, imipridones inhibit EZH1/2 in tumor cells in a manner that mimics H3K27M mutation supporting their role in anti-cancer efficacy. ONC201 and EZH2i share similar targets and actions on tumors. Synergistic combinations of imipridones plus EZH1/2i or imipridones, EZH2i and HDACi merit further investigation.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 3","pages":"1307-1320"},"PeriodicalIF":3.6,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982711/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143970810","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In-hospital outcomes of percutaneous ablative therapy for colorectal cancer liver metastasis in patients with and without frailty: nationwide inpatient sample analysis 2005-2020.","authors":"Yen-Jung Lu, Chien-Hsin Chen, En-Kwang Lin","doi":"10.62347/MQXG6358","DOIUrl":"https://doi.org/10.62347/MQXG6358","url":null,"abstract":"<p><p>Percutaneous ablative therapies are widely used to treat colorectal liver metastases (CRLM), particularly in patients who are not candidates for surgical resection. Frailty has been associated with poor outcomes in colorectal cancer (CRC) and liver resections. This study aimed to evaluate the clinical impact of frailty on short-term outcomes in patients undergoing percutaneous ablative therapies for CRLM. This population-based, retrospective study used data from the US Nationwide Inpatient Sample database (2005-2020). Adults aged ≥ 50 years diagnosed with CRLM who underwent percutaneous ablative therapies were included. Frailty was confirmed using the Hospital Frailty Risk Score (HFRS). Associations between frailty and in-hospital mortality, length of hospital stay (LOS), non-home discharge, total hospital charges, and postoperative complications were evaluated using univariate and multivariable regression analyses. A total of 670 patients (mean age: 66.3 years) were included, of whom 23% were categorized as frail (HFRS ≥ 5). Multivariable analysis showed that frail patients had significantly increased risks of complications (adjusted odds ratio [aOR] = 4.80, 95% confidence interval [CI]: 3.04-7.59), longer LOS (adjusted Beta [aBeta] = 1.69 days, 95% CI: 1.68-1.70), and higher total hospital charges (aBeta = $22.04 thousand, 95% CI: $21.92-$22.16). Complications with the highest risks in frail patients included, sepsis/shock (aOR = 17.39), surgical site infection (aOR = 3.55), respiratory failure/mechanical ventilation (aOR = 4.43), acute kidney injury (aOR = 9.37), and bleeding (aOR = 4.79). In conclusion, in adults aged ≥ 50 years undergoing percutaneous ablative therapies for CRLM, frailty independently predicted worse short-term outcomes, including higher complication rates, longer LOS, and increased hospital charges. The absence of detailed tumor characteristics and specific types of ablative therapy performed underscores the need for further research.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 3","pages":"1280-1290"},"PeriodicalIF":3.6,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982708/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of telemedicine on racial and ethnic disparities in oncologic care during the COVID-19 pandemic.","authors":"Brian D Cortese, Khalid Y Alkhatib, I Mitchell Harmatz, Katharine F Michel, Daniel J Lee, Thomas J Guzzo, David J Vaughn, Kelvin A Moses, Phillip M Pierorazio, Ruchika Talwar","doi":"10.62347/RNJS4301","DOIUrl":"https://doi.org/10.62347/RNJS4301","url":null,"abstract":"<p><p>Codification of COVID-19-era use of telemedicine as a permanent feature of US healthcare has been discussed as it may increase accessibility and equity. This study assesses whether telemedicine is associated with improved differential access to cancer care for racial and ethnic minorities. We conducted a cross-sectional analysis of the National Health Interview Survey from July 2020 to December 2021 and estimated prevalence of telemedicine utilization in both the study population (N=46,799) and in a subgroup of cancer patients (N=7,784). Complex survey-weighted multivariable Poisson regression identified patient-level predictors and estimated risk ratios (RR) for telemedicine receipt. Two-way interaction between cancer type and race and ethnicity assessed effect modification. Telemedicine prevalence was 35.5% [95% CI: 34.8%-36.2%] for the overall study population and 48.7% [95% CI: 47.0%-50.4%] for cancer patients. Weighted multivariable Poisson regression revealed that non-Hispanic Black (NHB) and non-Hispanic Asian (NHA) individuals had lower receipt compared to non-Hispanic White (NHB RR: 0.87, 95% CI: [0.83-0.92], P<0.01; NHA RR: 0.8, 95% CI: [0.74-0.86], P<0.01). This racial and ethnic disparity disappeared among cancer patients. Adjusted risk difference (ARD) analysis indicated no difference in decreased telemedicine utilization by cancer type except for breast cancer (NHB ARD: -0.16, 95% CI: [-0.27-(-0.05)], P=0.01) and lymphoma (Other ARD: -0.36, 95% CI: [-0.72-(-0.01)], P=0.05). Racial and ethnic disparities in telemedicine utilization decreased for cancer patients compared to the overall population. While racial and ethnic disparities persisted in two oncologic subgroups, telemedicine overall improved access and may increase equity in oncologic care.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 3","pages":"1224-1233"},"PeriodicalIF":3.6,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982731/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143958155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development and validation of a risk prediction model for lymph node metastasis in stage IA2-IIA1 cervical cancer based on laboratory parameters.","authors":"Yongli Hou, Lili Zhang, Hui Wang, Wenhao Wang, Min Hao","doi":"10.62347/EOXM6715","DOIUrl":"https://doi.org/10.62347/EOXM6715","url":null,"abstract":"<p><strong>Objective: </strong>To develop and validate a risk prediction model for lymph node metastasis (LNM) in stage IA2-IIA1 cervical cancer (CC) using laboratory parameters to aid in preoperative risk assessment and personalized treatment planning.</p><p><strong>Methods: </strong>A retrospective analysis was conducted on 624 patients treated between 2017 and 2023, divided into a training group (418 patients) and a validation group (206 patients). Clinical and laboratory data, including squamous cell carcinoma antigen (SCC-Ag), carcinoembryonic antigen (CEA), cancer antigen 125 (CA125), platelet count (PLT), fibrinogen (FIB), and C-reactive protein (CRP), were collected. Independent risk factors for LNM were identified using Least Absolute Shrinkage and Selection Operator (LASSO) regression. A predictive model was constructed and evaluated using receiver operating characteristic (ROC) curve analysis, decision curve analysis (DCA), and calibration curve.</p><p><strong>Results: </strong>SCC-Ag, CEA, CA125, PLT, FIB, and CRP were identified as significant predictors of LNM, with SCC-Ag demonstrating an AUC of 0.811 (sensitivity: 65.00%, specificity: 93.08%). The model achieved an AUC of 0.969 in the training group and 0.942 in the validation group, indicating robust generalizability and high predictive accuracy. DCA confirmed the model's clinical utility across a wide range of risk thresholds, and the calibration curve showed a good agreement between predicted and observed outcomes.</p><p><strong>Conclusions: </strong>This laboratory parameter-based risk prediction model is a reliable and practical tool for assessing LNM risk in stage IA2-IIA1 CC patients, supporting better clinical decision-making and reducing unnecessary interventions.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 3","pages":"1081-1095"},"PeriodicalIF":3.6,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143954624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Causal effect of interstitial lung disease on lung cancer risk in never-smokers and prognostic insights from Mendelian randomization and transcriptome analysis.","authors":"Limin Chi, Mengyan Li, Hanxing Zhou, Mien-Chie Hung, Wei-Jan Wang, Bo Wang, Xian Sun","doi":"10.62347/NJCQ2464","DOIUrl":"https://doi.org/10.62347/NJCQ2464","url":null,"abstract":"<p><strong>Introduction: </strong>The relationship between interstitial lung disease (ILD) and lung cancer in nonsmokers (LCINS) has garnered increasing interest. However, the causal associations and underlying pathogenesis between ILD and LCINS remain poorly understood.</p><p><strong>Methods: </strong>This research utilized a bidirectional two-sample Mendelian randomization (MR) method, utilizing forward MR analysis to assess the causal impact of ILD on LCINS and reverse MR analysis to evaluate the causal effect of LCINS on ILD. Additionally, transcriptome data and bioinformatics analyses were used to explore the associations between ILD and LCINS. An ILD-related gene signature (ILD risk score) was identified to examine its influence on the hallmark signaling pathways and the immune microenvironment in LCINS.</p><p><strong>Results: </strong>The study revealed a significant causal relationship between ILD and LCINS, with ILD increasing the risk of lung cancer in nonsmoking European populations. We developed a 5-gene risk model, which includes CD1A, CDH3, KRT6B, MMP1, and MMP10, via least absolute shrinkage and selection operator (LASSO) regression. The ILD risk score independently influences the prognosis of nonsmoking patients with lung cancer, and these five genes are also significantly associated with overall survival (OS) rates. Patients in the high-ILD risk subgroup exhibited significantly poorer survival rates. A highly accurate nomogram was developed to increase the clinical applicability of the ILD risk score. Additionally, the ILD risk scores were significantly correlated with hallmark signaling pathways and immune cell infiltration.</p><p><strong>Conclusions: </strong>This study suggested that ILD may have a positive causal effect on LCINS, with the ILD risk score serving as an effective predictor of the prognoses in LCINS patients. It is associated with tumor proliferation and the activation of metabolism-related signaling pathways. These findings also indicate that ILD may contribute to the occurrence and progression of LCINS through its influence on immune cell infiltration.</p>","PeriodicalId":7437,"journal":{"name":"American journal of cancer research","volume":"15 3","pages":"855-875"},"PeriodicalIF":3.6,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11982725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143965458","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}