Nucleus (Austin, Tex.)最新文献

Elucidating the nanoscopic organization and dynamics of the nuclear pore complex. 阐明核孔复合物的纳米级组织和动力学。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-06-04 DOI: 10.1080/19491034.2025.2510106

Kevin N Baumann, Eva Bertosin, Anders Barth, Cees Dekker, Roderick Y H Lim

{"title":"Elucidating the nanoscopic organization and dynamics of the nuclear pore complex.","authors":"Kevin N Baumann, Eva Bertosin, Anders Barth, Cees Dekker, Roderick Y H Lim","doi":"10.1080/19491034.2025.2510106","DOIUrl":"10.1080/19491034.2025.2510106","url":null,"abstract":"Due to its pivotal role as a regulator of nucleocytoplasmic transport, the structure and dynamic gating mechanism of the nuclear pore complex (NPC) is a subject of immense interest. Here, we report key recent advancements discussed at the Selective Transport Control in Biological and Biomimetic Nanopores meeting (Monte Verità, Switzerland, 2024) that gathered NPC experts from a range of disciplines. Novel insights were reported from cutting-edge super-resolution techniques that enable the direct interrogation of the NPC's dynamic central transporter; computational models that unravel the mechanisms of the selective barrier; and synthetic NPC mimics as valuable in vitro models for delineating NPC permeability and transport dynamics. Altogether, three major insights were highlighted: (i) the presence of dynamically organised nuclear transport pathways within the NPC, (ii) the role of nuclear transport receptors that enrich and reinforce the NPC's selective permeability barrier, and (iii) the ability of DNA origami nanostructures to mimic aspects of the NPC with unprecedented precision. Overall, the advancements marked a convergence in our understanding of NPC function by unraveling its dynamic gating mechanism at the nanoscale.","PeriodicalId":74323,"journal":{"name":"Nucleus (Austin, Tex.)","volume":"16 1","pages":"2510106"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143695/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear envelope components in vascular mechanotransduction: emerging roles in vascular health and disease. 血管机械转导中的核膜成分：在血管健康和疾病中的新作用。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-01-19 DOI: 10.1080/19491034.2025.2453752

Tung D Nguyen, Michael A Winek, Mihir K Rao, Shaiva P Dhyani, Monica Y Lee

{"title":"Nuclear envelope components in vascular mechanotransduction: emerging roles in vascular health and disease.","authors":"Tung D Nguyen, Michael A Winek, Mihir K Rao, Shaiva P Dhyani, Monica Y Lee","doi":"10.1080/19491034.2025.2453752","DOIUrl":"10.1080/19491034.2025.2453752","url":null,"abstract":"The vascular network, uniquely sensitive to mechanical changes, translates biophysical forces into biochemical signals for vessel function. This process relies on the cell's architectural integrity, enabling uniform responses to physical stimuli. Recently, the nuclear envelope (NE) has emerged as a key regulator of vascular cell function. Studies implicate nucleoskeletal elements (e.g. nuclear lamina) and the linker of nucleoskeleton and cytoskeleton (LINC) complex in force transmission, emphasizing nucleo-cytoskeletal communication in mechanotransduction. The nuclear pore complex (NPC) and its component proteins (i.e. nucleoporins) also play roles in cardiovascular disease (CVD) progression. We herein summarize evidence on the roles of nuclear lamina proteins, LINC complex members, and nucleoporins in endothelial and vascular cell mechanotransduction. Numerous studies attribute NE components in cytoskeletal-related cellular behaviors to insinuate dysregulation of nucleocytoskeletal feedback and nucleocytoplasmic transport as a mechanism of endothelial and vascular dysfunction, and hence implications for aging and vascular pathophysiology.","PeriodicalId":74323,"journal":{"name":"Nucleus (Austin, Tex.)","volume":"16 1","pages":"2453752"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear envelope and chromatin choreography direct cellular differentiation. 核膜和染色质编排直接影响细胞分化。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-02-12 DOI: 10.1080/19491034.2024.2449520

Anjitha Nair, Jayati Khanna, Jashan Kler, Rohith Ragesh, Kundan Sengupta

{"title":"Nuclear envelope and chromatin choreography direct cellular differentiation.","authors":"Anjitha Nair, Jayati Khanna, Jashan Kler, Rohith Ragesh, Kundan Sengupta","doi":"10.1080/19491034.2024.2449520","DOIUrl":"10.1080/19491034.2024.2449520","url":null,"abstract":"The nuclear envelope plays an indispensable role in the spatiotemporal organization of chromatin and transcriptional regulation during the intricate process of cell differentiation. This review outlines the distinct regulatory networks between nuclear envelope proteins, transcription factors and epigenetic modifications in controlling the expression of cell lineage-specific genes during differentiation. Nuclear lamina with its associated nuclear envelope proteins organize heterochromatin via Lamina-Associated Domains (LADs), proximal to the nuclear periphery. Since nuclear lamina is mechanosensitive, we critically examine the impact of extracellular forces on differentiation outcomes. The nuclear envelope is spanned by nuclear pore complexes which, in addition to their central role in transport, are associated with chromatin organization. Furthermore, mutations in the nuclear envelope proteins disrupt differentiation, resulting in developmental disorders. Investigating the underlying nuclear envelope controlled regulatory mechanisms of chromatin remodelling during lineage commitment will accelerate our fundamental understanding of developmental biology and regenerative medicine.","PeriodicalId":74323,"journal":{"name":"Nucleus (Austin, Tex.)","volume":"16 1","pages":"2449520"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143411944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Noncoding RNAs in nuclear organization. 核组织中的非编码rna。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-03-13 DOI: 10.1080/19491034.2025.2477848

Thembalami Dube, Dawn M Carone

引用次数: 0

RNA Pol-II transcripts in nucleolar associated domains of cancer cell nucleoli. 癌细胞核仁核仁相关区域的RNA Pol-II转录物。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-02-23 DOI: 10.1080/19491034.2025.2468597

Soumya Roy Chowdhury, Arunima Shilpi, Gary Felsenfeld

引用次数: 0

Closing the loops: chromatin loop dynamics after DNA damage. 闭合环：DNA损伤后染色质环动力学。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2024-12-25 DOI: 10.1080/19491034.2024.2438633

Pierre-Alexandre Vidi, Jing Liu, Keith Bonin, Kerry Bloom

引用次数: 0

Recent advances in nuclear actin research. 核肌动蛋白研究的最新进展。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-05-04 DOI: 10.1080/19491034.2025.2498643

Anikó Szabó, Péter Borkúti, Zoltán Kovács, Ildikó Kristó, Péter Vilmos

引用次数: 0

Correction. 修正。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-01-21 DOI: 10.1080/19491034.2024.2443274

引用次数: 0

Bridging-mediated compaction of mitotic chromosomes. 桥接介导的有丝分裂染色体的压实。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-05-09 DOI: 10.1080/19491034.2025.2497765

Giada Forte, Lora Boteva, Nick Gilbert, Peter R Cook, Davide Marenduzzo

引用次数: 0

Interplay of replication timing, DNA repair, and translesion synthesis in UV mutagenesis in yeast. 酵母紫外诱变中复制时间、DNA修复和翻译合成的相互作用。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-03-13 DOI: 10.1080/19491034.2025.2476935

Allysa Sewell, John J Wyrick

{"title":"Interplay of replication timing, DNA repair, and translesion synthesis in UV mutagenesis in yeast.","authors":"Allysa Sewell, John J Wyrick","doi":"10.1080/19491034.2025.2476935","DOIUrl":"10.1080/19491034.2025.2476935","url":null,"abstract":"Replication timing during S-phase impacts mutation rates in yeast and human cancers; however, the exact mechanism involved remains unclear. Here, we analyze the impact of replication timing on UV mutagenesis in Saccharomyces cerevisiae. Our analysis indicates that UV mutations are enriched in early-replicating regions of the genome in wild-type cells, but in cells deficient in global genomic-nucleotide excision repair (GG-NER), mutations are enriched in late-replicating regions. Analysis of UV damage maps revealed that cyclobutane pyrimidine dimers are enriched in late-replicating regions, but this enrichment is almost entirely due to repetitive ribosomal DNA. Complex mutations typically associated with TLS activity are also elevated in late-replicating regions in GG-NER deficient cells. We propose that UV mutagenesis is higher in early-replicating regions in repair-competent cells because there is less time to repair the lesion prior to undergoing replication. However, in the absence of GG-NER, increased TLS activity promotes UV mutagenesis in late-replicating regions.","PeriodicalId":74323,"journal":{"name":"Nucleus (Austin, Tex.)","volume":"16 1","pages":"2476935"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11913381/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143617679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0