Nucleus (Austin, Tex.)最新文献

Elucidating the nanoscopic organization and dynamics of the nuclear pore complex. 阐明核孔复合物的纳米级组织和动力学。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-06-04 DOI: 10.1080/19491034.2025.2510106

Kevin N Baumann, Eva Bertosin, Anders Barth, Cees Dekker, Roderick Y H Lim

{"title":"Elucidating the nanoscopic organization and dynamics of the nuclear pore complex.","authors":"Kevin N Baumann, Eva Bertosin, Anders Barth, Cees Dekker, Roderick Y H Lim","doi":"10.1080/19491034.2025.2510106","DOIUrl":"10.1080/19491034.2025.2510106","url":null,"abstract":"Due to its pivotal role as a regulator of nucleocytoplasmic transport, the structure and dynamic gating mechanism of the nuclear pore complex (NPC) is a subject of immense interest. Here, we report key recent advancements discussed at the Selective Transport Control in Biological and Biomimetic Nanopores meeting (Monte Verità, Switzerland, 2024) that gathered NPC experts from a range of disciplines. Novel insights were reported from cutting-edge super-resolution techniques that enable the direct interrogation of the NPC's dynamic central transporter; computational models that unravel the mechanisms of the selective barrier; and synthetic NPC mimics as valuable in vitro models for delineating NPC permeability and transport dynamics. Altogether, three major insights were highlighted: (i) the presence of dynamically organised nuclear transport pathways within the NPC, (ii) the role of nuclear transport receptors that enrich and reinforce the NPC's selective permeability barrier, and (iii) the ability of DNA origami nanostructures to mimic aspects of the NPC with unprecedented precision. Overall, the advancements marked a convergence in our understanding of NPC function by unraveling its dynamic gating mechanism at the nanoscale.","PeriodicalId":74323,"journal":{"name":"Nucleus (Austin, Tex.)","volume":"16 1","pages":"2510106"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12143695/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144217746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterogeneity as a feature: unraveling chromatin's role in nuclear mechanics. 异质性作为一个特征：揭示染色质在核力学中的作用。

IF 4.5

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-08-21 DOI: 10.1080/19491034.2025.2545037

Wessel S Rodenburg, Amy R Strom, Jorine M Eeftens

{"title":"Heterogeneity as a feature: unraveling chromatin's role in nuclear mechanics.","authors":"Wessel S Rodenburg, Amy R Strom, Jorine M Eeftens","doi":"10.1080/19491034.2025.2545037","DOIUrl":"https://doi.org/10.1080/19491034.2025.2545037","url":null,"abstract":"Mechanical forces are a ubiquitous feature of the cellular environment. These forces propagate to the nucleus, where the mechanical response is critical for cellular function and survival. In addition to the nuclear lamina and cytoskeletal connections, chromatin is a key structural and mechanoresponsive element which not only contributes to bulk stiffness but also dynamically adapts its organization in response to mechanical stress. Crucially, chromatin is not a uniform material - its organization and mechanical properties vary across time, cell state, and even within individual nuclei. This heterogeneity underpins compartmentalization, gene regulation, and potentially, disease states when disrupted. In this review, we summarize recent experimental advances that have illuminated chromatin's role in nuclear mechanics, emphasizing the importance of heterogeneity. We argue that an integrated, multiscale, and quantitative framework is essential for dissecting chromatin's mechanical contributions. By doing so, the field will be better positioned to link nuclear mechanics to functional biological outcomes.","PeriodicalId":74323,"journal":{"name":"Nucleus (Austin, Tex.)","volume":"16 1","pages":"2545037"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12372515/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144981656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear envelope and chromatin choreography direct cellular differentiation. 核膜和染色质编排直接影响细胞分化。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-02-12 DOI: 10.1080/19491034.2024.2449520

Anjitha Nair, Jayati Khanna, Jashan Kler, Rohith Ragesh, Kundan Sengupta

{"title":"Nuclear envelope and chromatin choreography direct cellular differentiation.","authors":"Anjitha Nair, Jayati Khanna, Jashan Kler, Rohith Ragesh, Kundan Sengupta","doi":"10.1080/19491034.2024.2449520","DOIUrl":"10.1080/19491034.2024.2449520","url":null,"abstract":"The nuclear envelope plays an indispensable role in the spatiotemporal organization of chromatin and transcriptional regulation during the intricate process of cell differentiation. This review outlines the distinct regulatory networks between nuclear envelope proteins, transcription factors and epigenetic modifications in controlling the expression of cell lineage-specific genes during differentiation. Nuclear lamina with its associated nuclear envelope proteins organize heterochromatin via Lamina-Associated Domains (LADs), proximal to the nuclear periphery. Since nuclear lamina is mechanosensitive, we critically examine the impact of extracellular forces on differentiation outcomes. The nuclear envelope is spanned by nuclear pore complexes which, in addition to their central role in transport, are associated with chromatin organization. Furthermore, mutations in the nuclear envelope proteins disrupt differentiation, resulting in developmental disorders. Investigating the underlying nuclear envelope controlled regulatory mechanisms of chromatin remodelling during lineage commitment will accelerate our fundamental understanding of developmental biology and regenerative medicine.","PeriodicalId":74323,"journal":{"name":"Nucleus (Austin, Tex.)","volume":"16 1","pages":"2449520"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11834525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143411944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Oncogenic lncRNA transgene transcription modulates epigenetic memory at a naïve chromosomal locus. 致癌lncRNA转基因转录调节naïve染色体位点的表观遗传记忆。

IF 4.5

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-07-31 DOI: 10.1080/19491034.2025.2534242

Sweta Sikder, Songjoon Baek, Yamini Dalal, Ganesan Arunkumar

{"title":"Oncogenic lncRNA transgene transcription modulates epigenetic memory at a naïve chromosomal locus.","authors":"Sweta Sikder, Songjoon Baek, Yamini Dalal, Ganesan Arunkumar","doi":"10.1080/19491034.2025.2534242","DOIUrl":"10.1080/19491034.2025.2534242","url":null,"abstract":"Maintaining genome integrity is essential for the proper functioning and development of organisms. An intriguing aspect is that neocentromeres can form at non-centromeric sites. CENP-A, a key epigenetic marker of centromeres, is often mislocalized to ectopic sites in cancers when overexpressed. Its deposition on centromeres relies on transcription of centromeric non-coding RNAs. Subsequently, ectopic CENP-A is frequently found at transcriptionally active and chromosome breakpoint regions. We previously engineered a stable ectopic CENP-A site on a naïve chromosome by overexpressing PCAT2, a non-centromeric oncogenic lncRNA that recruits CENP-A to its transcribing locus. We tracked cells with this transgene to analyze the longevity of ectopic CENP-A. We discovered that this induced epigenetic memory was lost due to suppression by epigenetic silencing mechanisms, restoring CENP-A to previous levels. These findings suggest that cells have mechanisms to prevent neocentromere formation at ectopic sites by suppressing transcription unless selective pressure favors it.","PeriodicalId":74323,"journal":{"name":"Nucleus (Austin, Tex.)","volume":"16 1","pages":"2534242"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12320815/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144762577","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nuclear envelope components in vascular mechanotransduction: emerging roles in vascular health and disease. 血管机械转导中的核膜成分：在血管健康和疾病中的新作用。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-01-19 DOI: 10.1080/19491034.2025.2453752

Tung D Nguyen, Michael A Winek, Mihir K Rao, Shaiva P Dhyani, Monica Y Lee

{"title":"Nuclear envelope components in vascular mechanotransduction: emerging roles in vascular health and disease.","authors":"Tung D Nguyen, Michael A Winek, Mihir K Rao, Shaiva P Dhyani, Monica Y Lee","doi":"10.1080/19491034.2025.2453752","DOIUrl":"10.1080/19491034.2025.2453752","url":null,"abstract":"The vascular network, uniquely sensitive to mechanical changes, translates biophysical forces into biochemical signals for vessel function. This process relies on the cell's architectural integrity, enabling uniform responses to physical stimuli. Recently, the nuclear envelope (NE) has emerged as a key regulator of vascular cell function. Studies implicate nucleoskeletal elements (e.g. nuclear lamina) and the linker of nucleoskeleton and cytoskeleton (LINC) complex in force transmission, emphasizing nucleo-cytoskeletal communication in mechanotransduction. The nuclear pore complex (NPC) and its component proteins (i.e. nucleoporins) also play roles in cardiovascular disease (CVD) progression. We herein summarize evidence on the roles of nuclear lamina proteins, LINC complex members, and nucleoporins in endothelial and vascular cell mechanotransduction. Numerous studies attribute NE components in cytoskeletal-related cellular behaviors to insinuate dysregulation of nucleocytoskeletal feedback and nucleocytoplasmic transport as a mechanism of endothelial and vascular dysfunction, and hence implications for aging and vascular pathophysiology.","PeriodicalId":74323,"journal":{"name":"Nucleus (Austin, Tex.)","volume":"16 1","pages":"2453752"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017886","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNA Pol-II transcripts in nucleolar associated domains of cancer cell nucleoli. 癌细胞核仁核仁相关区域的RNA Pol-II转录物。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-02-23 DOI: 10.1080/19491034.2025.2468597

Soumya Roy Chowdhury, Arunima Shilpi, Gary Felsenfeld

引用次数: 0

Noncoding RNAs in nuclear organization. 核组织中的非编码rna。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-03-13 DOI: 10.1080/19491034.2025.2477848

Thembalami Dube, Dawn M Carone

引用次数: 0

Interplay between microtubule interactome, myonuclei mechanotransduction, and positioning in myopathies. 肌病中微管相互作用组、肌核机械转导和定位之间的相互作用。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-07-03 DOI: 10.1080/19491034.2025.2524909

Léa Castellano, Damien Caillol, Nathalie Streichenberger, Vincent Gache

{"title":"Interplay between microtubule interactome, myonuclei mechanotransduction, and positioning in myopathies.","authors":"Léa Castellano, Damien Caillol, Nathalie Streichenberger, Vincent Gache","doi":"10.1080/19491034.2025.2524909","DOIUrl":"10.1080/19491034.2025.2524909","url":null,"abstract":"Myofibers are the building block of skeletal muscle cells providing its capacity to contract and produce movement. The microtubule (MT) network sustains myofiber formation and its spatial organization is remodel during myofiber formation. This muscle-related MT network with specific partners, actively drive myonuclei localization in myofiber. In pathological conditions, myonuclei and MT patterning are affected and contribute to skeletal muscle mis-functionality. In this review, we classified myopathies depending on myonuclei positioning within myofibers and reported that 72% of myopathies exhibit myonuclei positioning alterations. We explored how this impairment can be analyzed according to muscle disease development, MT alterations and association with various partners. We highlighted how MT modifications impact muscle-specific mechanotransduction status and myofiber functional integrity. We then reported genes involved in myonuclei shape and positioning maintenance. Finally, we discussed technical contribution advances in the field to improve knowledge on muscle physiology and its challenges in disease context.","PeriodicalId":74323,"journal":{"name":"Nucleus (Austin, Tex.)","volume":"16 1","pages":"2524909"},"PeriodicalIF":0.0,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12233876/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144562222","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Recent advances in nuclear actin research. 核肌动蛋白研究的最新进展。

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-05-04 DOI: 10.1080/19491034.2025.2498643

Anikó Szabó, Péter Borkúti, Zoltán Kovács, Ildikó Kristó, Péter Vilmos

引用次数: 0

TET dioxygenases localize at splicing speckles and promote RNA splicing. TET双加氧酶定位于剪接斑点并促进RNA剪接。

IF 4.5

Nucleus (Austin, Tex.) Pub Date : 2025-12-01 Epub Date: 2025-07-27 DOI: 10.1080/19491034.2025.2536902

Florian D Hastert, Jasmin Weber, Christina Bauer, Andreas Zhadan, Deepanshu N D Singh, Thomas C Dix, Roland Arnold, Sergey Bessonov, Matthias Soller, Heinrich Leonhardt, M Cristina Cardoso, Maria Arroyo

{"title":"TET dioxygenases localize at splicing speckles and promote RNA splicing.","authors":"Florian D Hastert, Jasmin Weber, Christina Bauer, Andreas Zhadan, Deepanshu N D Singh, Thomas C Dix, Roland Arnold, Sergey Bessonov, Matthias Soller, Heinrich Leonhardt, M Cristina Cardoso, Maria Arroyo","doi":"10.1080/19491034.2025.2536902","DOIUrl":"10.1080/19491034.2025.2536902","url":null,"abstract":"The dynamic regulation of RNA metabolism plays a crucial part in cellular function, with emerging evidence suggesting an important role for RNA modifications in this process. This study explores the relationship between RNA splicing and the TET dioxygenase activity, shedding light on the role of hm5C (RNA 5-hydroxymethylcytosine), and TET proteins in RNA metabolism. Integrating data from mass spectrometry, AlphaFold structural modeling, microscopic analysis, and different functional assays, including in vitro splicing, TET proteins were found to regulate splicing. We show that TET1, TET2, and TET3 interact with the splicing factors U2AF1 and U2AF2. Interestingly, TET dioxygenases localize in splicing speckles in mammalian and Drosophila cells. TET speckles association was found to be RNA-dependent, but also rely on its interaction with splicing factors. Furthermore, cellular splicing assays revealed that all three TET proteins promote splicing efficiency independent of their catalytic activity. Interestingly, though, the oxidation of m5C to hm5C restores splicing efficiency in vitro. The latter highlights the regulatory role of cytosine modifications in RNA metabolism. These findings provide insights into the complex interplay between RNA modifications and splicing, suggesting a multifaceted role for TET proteins in RNA metabolism beyond their canonical function in the oxidation of 5mC in DNA.","PeriodicalId":74323,"journal":{"name":"Nucleus (Austin, Tex.)","volume":"16 1","pages":"2536902"},"PeriodicalIF":4.5,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12309557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144735916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0