Nucleus (Austin, Tex.)最新文献_第10页

Subnuclear distribution of proteins: Links with genome architecture. 蛋白质的核下分布：与基因组结构的联系

Nucleus (Austin, Tex.) Pub Date : 2018-01-01 Epub Date: 2017-09-14 DOI: 10.1080/19491034.2017.1361578

Fouziya R Shah, Younus A Bhat, Ajazul H Wani

引用次数: 0

Updated clinical overview on cardiac laminopathies: an electrical and mechanical disease. 心脏板层病的最新临床综述:一种电和机械疾病。

Nucleus (Austin, Tex.) Pub Date : 2018-01-01 DOI: 10.1080/19491034.2018.1489195

G Peretto, S Sala, S Benedetti, C Di Resta, L Gigli, M Ferrari, P Della Bella

引用次数: 31

Polycystic ovary syndrome in familial partial lipodystrophy type 2 (FPLD2): basic and clinical aspects. 家族性部分脂肪营养不良2型(FPLD2)多囊卵巢综合征:基础和临床方面。

Nucleus (Austin, Tex.) Pub Date : 2018-01-01 DOI: 10.1080/19491034.2018.1509659

Alessandra Gambineri, Laura Zanotti

引用次数: 7

Mechano-adaptation of the stem cell nucleus. 干细胞细胞核的机械适应性。

Nucleus (Austin, Tex.) Pub Date : 2018-01-01 Epub Date: 2017-11-13 DOI: 10.1080/19491034.2017.1371398

Su-Jin Heo, Brian D Cosgrove, Eric N Dai, Robert L Mauck

引用次数: 33

Extrusion without a motor: a new take on the loop extrusion model of genome organization. 无马达挤出：基因组组织环挤出模型的新进展。

Nucleus (Austin, Tex.) Pub Date : 2018-01-01 DOI: 10.1080/19491034.2017.1421825

C A Brackley, J Johnson, D Michieletto, A N Morozov, M Nicodemi, P R Cook, D Marenduzzo

{"title":"Extrusion without a motor: a new take on the loop extrusion model of genome organization.","authors":"C A Brackley, J Johnson, D Michieletto, A N Morozov, M Nicodemi, P R Cook, D Marenduzzo","doi":"10.1080/19491034.2017.1421825","DOIUrl":"10.1080/19491034.2017.1421825","url":null,"abstract":"Chromatin loop extrusion is a popular model for the formation of CTCF loops and topological domains. Recent HiC data have revealed a strong bias in favour of a particular arrangement of the CTCF binding motifs that stabilize loops, and extrusion is the only model to date which can explain this. However, the model requires a motor to generate the loops, and although cohesin is a strong candidate for the extruding factor, a suitable motor protein (or a motor activity in cohesin itself) has yet to be found. Here we explore a new hypothesis: that there is no motor, and thermal motion within the nucleus drives extrusion. Using theoretical modelling and computer simulations we ask whether such diffusive extrusion could feasibly generate loops. Our simulations uncover an interesting ratchet effect (where an osmotic pressure promotes loop growth), and suggest, by comparison to recent in vitro and in vivo measurements, that diffusive extrusion can in principle generate loops of the size observed in the data. Extra View on : C. A. Brackley, J. Johnson, D. Michieletto, A. N. Morozov, M. Nicodemi, P. R. Cook, and D. Marenduzzo \"Non-equilibrium chromosome looping via molecular slip-links\", Physical Review Letters 119 138101 (2017).","PeriodicalId":74323,"journal":{"name":"Nucleus (Austin, Tex.)","volume":" ","pages":"95-103"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/9a/cc/kncl-09-01-1421825.PMC5973195.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35707134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Myosin VI in the nucleus of neurosecretory PC12 cells: Stimulation-dependent nuclear translocation and interaction with nuclear proteins. 神经分泌型 PC12 细胞核中的肌球蛋白 VI：刺激依赖性核转运及与核蛋白的相互作用

Nucleus (Austin, Tex.) Pub Date : 2018-01-01 DOI: 10.1080/19491034.2017.1421881

Lukasz Majewski, Jolanta Nowak, Magdalena Sobczak, Olena Karatsai, Serhiy Havrylov, Robert Lenartowski, Malgorzata Suszek, Marta Lenartowska, Maria Jolanta Redowicz

{"title":"Myosin VI in the nucleus of neurosecretory PC12 cells: Stimulation-dependent nuclear translocation and interaction with nuclear proteins.","authors":"Lukasz Majewski, Jolanta Nowak, Magdalena Sobczak, Olena Karatsai, Serhiy Havrylov, Robert Lenartowski, Malgorzata Suszek, Marta Lenartowska, Maria Jolanta Redowicz","doi":"10.1080/19491034.2017.1421881","DOIUrl":"10.1080/19491034.2017.1421881","url":null,"abstract":"Myosin VI (MVI) is a unique actin-based motor protein moving towards the minus end of actin filaments, in the opposite direction than other known myosins. Besides well described functions of MVI in endocytosis and maintenance of Golgi apparatus, there are few reports showing its involvement in transcription. We previously demonstrated that in neurosecretory PC12 cells MVI was present in the cytoplasm and nucleus, and its depletion caused substantial inhibition of cell migration and proliferation. Here, we show an increase in nuclear localization of MVI upon cell stimulation, and identification of potential nuclear localization (NLS) and nuclear export (NES) signals within MVI heavy chain. These signals seem to be functional as the MVI nuclear presence was affected by the inhibitors of nuclear import (ivermectin) and export (leptomycin B). In nuclei of stimulated cells, MVI colocalized with active RNA polymerase II, BrUTP-containing transcription sites and transcription factor SP1 as well as SC35 and PML proteins, markers of nuclear speckles and PML bodies, respectively. Mass spectrometry analysis of samples of a GST-pull-down assay with the MVI tail domain as a \"bait\" identified several new potential MVI binding partners. Among them are proteins involved in transcription and post-transcriptional processes. We confirmed interaction of MVI with heterogeneous nuclear ribonucleoprotein U (hnRNPU) and nucleolin, proteins involved in pre-mRNA binding and transport, and nucleolar function, respectively. Our data provide an insight into mechanisms of involvement of MVI in nuclear processes via interaction with nuclear proteins and support a notion for important role(s) for MVI in gene expression.","PeriodicalId":74323,"journal":{"name":"Nucleus (Austin, Tex.)","volume":" ","pages":"125-141"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5973263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35701153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Differential staining of peripheral nuclear chromatin with Acridine orange implies an A-form epichromatin conformation of the DNA. 用吖啶橙鉴别染色外周核染色质表明DNA呈a型外染色质构象。

Nucleus (Austin, Tex.) Pub Date : 2018-01-01 DOI: 10.1080/19491034.2018.1431081

Jekaterina Erenpreisa, Jekabs Krigerts, Kristine Salmina, Turs Selga, Hermanis Sorokins, Talivaldis Freivalds

{"title":"Differential staining of peripheral nuclear chromatin with Acridine orange implies an A-form epichromatin conformation of the DNA.","authors":"Jekaterina Erenpreisa, Jekabs Krigerts, Kristine Salmina, Turs Selga, Hermanis Sorokins, Talivaldis Freivalds","doi":"10.1080/19491034.2018.1431081","DOIUrl":"https://doi.org/10.1080/19491034.2018.1431081","url":null,"abstract":"The chromatin observed by conventional electron microscopy under the nuclear envelope constitutes a single layer of dense 30-35 nm granules, while ∼30 nm fibrils laterally attached to them, form large patches of lamin-associated domains (LADs). This particular surface \"epichromatin\" can be discerned by specific (H2A+H2B+DNA) conformational antibody at the inner nuclear envelope and around mitotic chromosomes. In order to differentiate the DNA conformation of the peripheral chromatin we applied an Acridine orange (AO) DNA structural test involving RNAse treatment and the addition of AO after acid pre-treatment. MCF-7 cells treated in this way revealed yellow/red patches of LADs attached to a thin green nuclear rim and with mitotic chromosomes outlined in green, topologically corresponding to epichromatin epitope staining by immunofluorescence. Differentially from LADs, the epichromatin was unable to provide metachromatic staining by AO, unless thermally denatured at 94oC. DNA enrichment in GC stretches has been recently reported for immunoprecipitated ∼ 1Kb epichromatin domains. Together these data suggest that certain epichromatin segments assume the relatively hydrophobic DNA A-conformation at the nuclear envelope and surface of mitotic chromosomes, preventing AO side dimerisation. We hypothesize that epichromatin domains form nucleosome superbeads. Hydrophobic interactions stack these superbeads and align them at the nuclear envelope, while repulsing the hydrophilic LADs. The hydrophobicity of epichromatin explains its location at the surface of mitotic chromosomes and its function in mediating chromosome attachment to the restituting nuclear envelope during telophase.","PeriodicalId":74323,"journal":{"name":"Nucleus (Austin, Tex.)","volume":" ","pages":"171-181"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1080/19491034.2018.1431081","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35761772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 11

From dynamic chromatin architecture to DNA damage repair and back. 从动态染色质结构到DNA损伤修复再回来。

Nucleus (Austin, Tex.) Pub Date : 2018-01-01 DOI: 10.1080/19491034.2017.1419847

Emmanuelle Fabre, Christophe Zimmer

引用次数: 13

Invertebrate models of lamin diseases. 层粘连蛋白疾病的无脊椎动物模型。

Nucleus (Austin, Tex.) Pub Date : 2018-01-01 DOI: 10.1080/19491034.2018.1454166

Ryszard Rzepecki, Yosef Gruenbaum

引用次数: 8

Analysis of RNA-Seq datasets reveals enrichment of tissue-specific splice variants for nuclear envelope proteins. RNA-Seq数据集的分析揭示了核膜蛋白的组织特异性剪接变异体的富集。

Nucleus (Austin, Tex.) Pub Date : 2018-01-01 DOI: 10.1080/19491034.2018.1469351

Charlotte Capitanchik, Charles R Dixon, Selene K Swanson, Laurence Florens, Alastair R W Kerr, Eric C Schirmer

{"title":"Analysis of RNA-Seq datasets reveals enrichment of tissue-specific splice variants for nuclear envelope proteins.","authors":"Charlotte Capitanchik, Charles R Dixon, Selene K Swanson, Laurence Florens, Alastair R W Kerr, Eric C Schirmer","doi":"10.1080/19491034.2018.1469351","DOIUrl":"10.1080/19491034.2018.1469351","url":null,"abstract":"Laminopathies yield tissue-specific pathologies, yet arise from mutation of ubiquitously-expressed genes. A little investigated hypothesis to explain this is that the mutated proteins or their partners have tissue-specific splice variants. To test this, we analyzed RNA-Seq datasets, finding novel isoforms or isoform tissue-specificity for: Lap2, linked to cardiomyopathy; Nesprin 2, linked to Emery-Dreifuss muscular dystrophy and Lmo7, that regulates the Emery-Dreifuss muscular dystrophy linked emerin gene. Interestingly, the muscle-specific Lmo7 exon is rich in serine phosphorylation motifs, suggesting regulatory function. Muscle-specific splice variants in non-nuclear envelope proteins linked to other muscular dystrophies were also found. Nucleoporins tissue-specific variants were found for Nup54, Nup133, Nup153 and Nup358/RanBP2. RT-PCR confirmed novel Lmo7 and RanBP2 variants and specific knockdown of the Lmo7 variantreduced myogenic index. Nuclear envelope proteins were enriched for tissue-specific splice variants compared to the rest of the genome, suggesting that splice variants contribute to its tissue-specific functions.","PeriodicalId":74323,"journal":{"name":"Nucleus (Austin, Tex.)","volume":" ","pages":"410-430"},"PeriodicalIF":0.0,"publicationDate":"2018-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7000147/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36232086","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0