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[SOME REACTIONS OF THE REGIONAL LYMPH NODES OF RATS AFTER IMPLANTATION OF MULTIPOTENT STROMAL CELLS ADSORBED ON POLYHYDROXYALKANOATE INTO A BONE TISSUE DEFECT]. [多羟基烷酸吸附的多能基质细胞植入骨组织缺损后大鼠局部淋巴结的一些反应]。
Morfologiia (Saint Petersburg, Russia) Pub Date : 2016-01-01
I V Maiborodin, V A Matveyeva, R V Maslov, N V Onopriyenko, I V Kuznetsova, G A Chastikin, A A Anikeyev
{"title":"[SOME REACTIONS OF THE REGIONAL LYMPH NODES OF RATS AFTER IMPLANTATION OF MULTIPOTENT STROMAL CELLS ADSORBED ON POLYHYDROXYALKANOATE INTO A BONE TISSUE DEFECT].","authors":"I V Maiborodin,&nbsp;V A Matveyeva,&nbsp;R V Maslov,&nbsp;N V Onopriyenko,&nbsp;I V Kuznetsova,&nbsp;G A Chastikin,&nbsp;A A Anikeyev","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The reactions of the regional lymph nodes, caused\u0000by implantation of the autologous multipotent\u0000stromal cells of bone marrow origin (AMSCBMO)\u0000to accelerate the healing of mandibular bone\u0000defect were studied by fluorescent microscopy in\u0000inbred male Wag rats aged 6 months (n=62). After\u0000the introduction of polyhydroxyalkanoate transplant\u0000containing adsorbed AMSCBMO with a transfected\u0000Green Fluorescent Protein (GFP) gene into a\u0000damaged bone area, the lymphoid nodules in\u0000submandibular lymph nodes demonstrated the\u0000appearance of numerous large macrophages\u0000containing multiple oval fluorescent inclusions in\u0000the cytoplasm. The number of these macrophages\u0000increased within 2 weeks after surgery and then\u0000began to decline. Apparently, AMSCBMO\u0000introduced in this way, were partially absorbed by\u0000macrophages. After destruction of the structures\u0000formed from AMSCBMO, the debris was also\u0000phagocytized by macrophages. In either case,\u0000these macrophages appeared in the germinal\u0000centers of lymphoid nodules in lymph nodes,\u0000where the induction of immune responses against\u0000DNA and GFP protein was probable.</p>","PeriodicalId":74226,"journal":{"name":"Morfologiia (Saint Petersburg, Russia)","volume":"149 2","pages":"21-6"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36418175","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[REPARATIVE OSTEOGENESIS AND ANGIOGENESIS UNDER THE CONDITIONS OF TRANSOSSEOUS OSTEOSYNTHESIS AND THE EXPOSURE TO LOW-INTENSITY LASER RADIATION]. [经骨成骨和低强度激光照射条件下的修复性成骨和血管生成]。
Morfologiia (Saint Petersburg, Russia) Pub Date : 2016-01-01
Yu M Iryanov, N A Kiryanov
{"title":"[REPARATIVE OSTEOGENESIS AND ANGIOGENESIS UNDER THE CONDITIONS OF TRANSOSSEOUS OSTEOSYNTHESIS AND THE EXPOSURE TO LOW-INTENSITY LASER RADIATION].","authors":"Yu M Iryanov,&nbsp;N A Kiryanov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The aim of the study was the morphological\u0000analysis of the effect of exposure to low-intensity\u0000infrared laser radiation on reparative osteogenesis\u0000and angiogenesis in bone regenerate formed\u0000during the treatment of fracture under the\u0000conditions of transosseous osteosynthesis. In the\u0000experiment on rats in control (n=16) and\u0000experimental (n=16) groups the fracture of the tibia\u0000was modeled, and the reposition and fixation of\u0000bone fragments with a device for transosseous\u0000osteosynthesis was carried out. In animals of the\u0000experimental group, the area of the fracture was\u0000exposed to pulsed infrared laser radiation of low\u0000intensity. In the group of control animals, similar\u0000exposure was imitated. The operated bones were\u0000studied using radiography, light and electron\u0000microscopy, x-ray electron probe microanalysis. It\u0000was found that the sessions of laser irradiation\u0000reduced the severity of the inflammatory process,\u0000stimulated fibrillogenesis and endovascular\u0000capillary growth, accelerated the compaction of the\u0000newly formed bone, increased the degree of its\u0000maturity, with the fracture healing occurring by\u0000primary-type mechanism. Laser therapy of the\u0000fracture area provides for the formation of bone\u0000regenerate and bone fragment unification at an\u0000earlier date.</p>","PeriodicalId":74226,"journal":{"name":"Morfologiia (Saint Petersburg, Russia)","volume":"149 2","pages":"36-41"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36418177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[THE CYTOARCHITECTONICS AND NEURONAL ORGANIZATION OF THE BASOLATERAL NUCLEUS OF THE AMYGDALA OF THE BRAIN IN ALCOHOL-PREFERRING AND ALCOHOLNONPREFERRING RATS]. [嗜酒和不嗜酒大鼠大脑杏仁核基底外侧核的细胞结构和神经元组织]。
Morfologiia (Saint Petersburg, Russia) Pub Date : 2016-01-01
A V Akhmadeyev, L B Kalimullina
{"title":"[THE CYTOARCHITECTONICS AND NEURONAL ORGANIZATION OF THE BASOLATERAL NUCLEUS OF THE AMYGDALA OF THE BRAIN IN ALCOHOL-PREFERRING AND ALCOHOLNONPREFERRING RATS].","authors":"A V Akhmadeyev,&nbsp;L B Kalimullina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>Analysis of cytoarchitectonics of the basolateral\u0000nucleus (BLN) of the brain amygdala was\u0000performed in cresyl violetstained frontal paraffin\u0000sections of the brain in 10 alcoholpreferring (AP)\u0000and 10 alcohol-nonpreferring (ANP) rats (with an\u0000equal number of male and female animals in each\u0000group). The presence of large and small neurons\u0000was detected in BLN. Most of the large neurons in\u0000AP rats had the character of chromoneutral and\u0000moderately chromophilic cells, while in ANP rats\u0000these cells were moderately chromophobic.\u0000Application of Golgi method demonstrated that the\u0000equivalents of large neurons were long-axonal\u0000densely branched pyramid-like neurons, and those\u0000of small-sized neurons — short-axonal neurons.\u0000The determination of the ratio of large and smallsized\u0000neurons showed that in AP rats the\u0000proportion of latter was 12.3±0.6%, while in the\u0000ANP rats it was significantly greater —\u000019.70±0.23%. These results help to explain the\u0000previously obtained data on larger specific area of\u0000BLN in amygdala of ANP rats by the presence of\u0000greater number of interneurons than in AP rats.</p>","PeriodicalId":74226,"journal":{"name":"Morfologiia (Saint Petersburg, Russia)","volume":"150 4","pages":"20-3"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36422163","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[HISTOLOGICAL CHANGES IN THE KNEE JOINT SYNOVIAL FLUID IN EXPERIMENTAL MODELING OF OSTEOARTHROSIS AND ORTHOPEDICBONE LENGTHENING IN DOGS]. [犬骨关节病和矫形骨延长实验模型中膝关节滑液的组织学变化]。
Morfologiia (Saint Petersburg, Russia) Pub Date : 2016-01-01
T A Stupina, M M Shchudlo, N A Shchudlo
{"title":"[HISTOLOGICAL CHANGES IN THE KNEE JOINT SYNOVIAL FLUID IN EXPERIMENTAL MODELING OF OSTEOARTHROSIS AND ORTHOPEDICBONE LENGTHENING IN DOGS].","authors":"T A Stupina,&nbsp;M M Shchudlo,&nbsp;N A Shchudlo","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The experiment was aimed at studying the\u0000structural reorganization of the knee joint synovium\u0000in dogs with modeling of primary osteoarthrosis\u0000(MOP) and tibial lengthening (TL). The methods of\u0000light microscopy and histomorphometry of semithin\u0000epoxy sections were used to assess the thickness\u0000of synovial surface layer, number of synoviocyte\u0000layers, characterize the cells, to measure the\u0000numerical density of vessels, and to define the\u0000changes of the nerves and cell composition of the\u0000subsynovial layer. In MOP-group, the\u0000predominance of type А synoviocytes,\u0000hypovascularity and irreversible changes in the\u0000nerves of subsynovial sheath were observed. In\u0000TL-group, the diversity of type В synoviocyte forms\u0000and sizes, hypervascularity of subsynovial layer\u0000and signs of its re-innervation were detected. It is\u0000concluded that the hyperplastic synoviopathy is\u0000possible in cases of both hypo- and\u0000hypervascularization of subsinovial layer.</p>","PeriodicalId":74226,"journal":{"name":"Morfologiia (Saint Petersburg, Russia)","volume":"149 2","pages":"42-6"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36422240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[AGE DYNAMICS OF THE MAIN SOMATOMETRIC PARAMETERS IN ADOLESCENT STUDENTS BORN IN THE CITY OF MAGADAN]. [马加丹市青少年学生主要身体测量参数的年龄动态]。
Morfologiia (Saint Petersburg, Russia) Pub Date : 2016-01-01
I V Averyanova, A L Maksimov
{"title":"[AGE DYNAMICS OF THE MAIN SOMATOMETRIC PARAMETERS IN ADOLESCENT STUDENTS BORN IN THE CITY OF MAGADAN].","authors":"I V Averyanova,&nbsp;A L Maksimov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>In the period from 2005 to 2013 the age dynamics\u0000of indices of physical development was studied in\u00001466 adolescent students aged from 17 to 21\u0000years born in Magadan. Studies have shown that\u0000the growth of the body length in boys in Magadan\u0000stopped at 17 years, whereas in the period of 19,\u000020 and 21 years, an increase in the body mass and\u0000chest circumference relative to the length of the\u0000body was detected, which, in turn, lead to an\u0000increase in the strength of the physique. All of the\u0000surveyed youths were characterized by a\u0000proportional and harmonious types of physical\u0000development.</p>","PeriodicalId":74226,"journal":{"name":"Morfologiia (Saint Petersburg, Russia)","volume":"149 2","pages":"62-7"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36422247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[CHANGES OF SPINAL MOTOR NEURONS IN MICE AFTER А SPACE FLIGHT]. [А航天飞行后小鼠脊髓运动神经元的变化]。
Morfologiia (Saint Petersburg, Russia) Pub Date : 2016-01-01
V V Porseva, V V Shilkin, A A Strelkov, I B Krasnov, P M Masliukov
{"title":"[CHANGES OF SPINAL MOTOR NEURONS IN MICE AFTER А SPACE FLIGHT].","authors":"V V Porseva,&nbsp;V V Shilkin,&nbsp;A A Strelkov,&nbsp;I B Krasnov,&nbsp;P M Masliukov","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The expression of choline acetyltransferase\u0000(ChAT), neurofilament (NF) protein 200 kDa,\u0000calbindin (CAB) 28 kDa, neuronal NO-synthase\u0000(nNOS), caspase 3, Ki-67 was studied in motor\u0000neurons from TIII–TV segments of the spinal cord\u0000in C57/ BL6 male mice by immunohistochemical\u0000methods 12 h after a 30 days-long space flight on\u0000the Bion-M1 biosatellite. Mice living under standard\u0000vivarium conditions served as a control. The\u0000motoneurons of experimental animals\u0000demonstrated the reactive changes that were\u0000manifested by the increase of their size, decrease\u0000in the number of subpopulations expressing ChAT\u0000and NF, increase of subpopulations containing\u0000CAB, appearance of motor neurons expressing\u0000nNOS, caspase-3, and the absence of Ki-67. The\u0000lack of proliferating gliocytes, the preservation of\u0000nucleolar component indicate the absence of\u0000necrobiotic changes characteristic of necrosis. The\u0000cytoplasmic vacuolization was observed in\u0000thionine-stained CAB-immunoreactive neurons.\u0000The disintegration of motor neurons into fragments\u0000resembling the apoptotic bodies was observed in\u0000single cells that contained caspase-3.</p>","PeriodicalId":74226,"journal":{"name":"Morfologiia (Saint Petersburg, Russia)","volume":"150 4","pages":"50-4"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36423002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[EXPRESSION OF DOUBLECORTIN AND NeuN IN THE DEVELOPING CEREBELLAR NEURONS IN RAT]. 大鼠发育中的小脑神经元中双皮质素和神经元的表达。
Morfologiia (Saint Petersburg, Russia) Pub Date : 2016-01-01
S M Zimatkin, O A Karniushko
{"title":"[EXPRESSION OF DOUBLECORTIN AND NeuN IN THE DEVELOPING CEREBELLAR NEURONS IN RAT].","authors":"S M Zimatkin,&nbsp;O A Karniushko","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>This work was performed on the offspring of 5 outbred female albino rats to give a comparative immunohistochemical evaluation of doublecortin (DCX) and NeuN expression in the neurons of the cerebellar cortex and nucleus interpositus in the early postnatal ontogenesis (postnatal days 2-15). DCX expression was detected in postmitotic neurons of the external granular layer and migrating neurons of the cerebellar cortex. At postnatal days 2 and 7 DCX expression in neocerebellum was higher than in paleocerebellum. NeuN expression was found to appear in migrating granule neurons, and reach the maximum in mature neurons of internal granular layer. DCX expression was not detected in Purkinje cells and in the nucleus interpositus of the cerebellum. In neurons of the nucleus interpositus the expression of NeuN progressively increased from postnatal days 2 to 15. Thus, a comparative immunohistochemical study of the dynamics of the expression of the pair of molecular markers studied proved to be an effective way of the assessment of the development of granular neurons of the cerebellum in early postnatal ontogenesis.</p>","PeriodicalId":74226,"journal":{"name":"Morfologiia (Saint Petersburg, Russia)","volume":"149 1","pages":"38-42"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34728325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[CHANGES OF THE CONTENT OF DLL4 AND Jag-1 ANGIOGENESIS REGULATORS IN HUMAN DERMIS IN ONTOGENESIS]. 人体真皮血管生成调节因子DLL4和jag1含量的变化。
Morfologiia (Saint Petersburg, Russia) Pub Date : 2016-01-01
N N Golubtzova, O V Vasiliyeva, V V Petrov, A G Gunin
{"title":"[CHANGES OF THE CONTENT OF DLL4 AND Jag-1 ANGIOGENESIS REGULATORS IN HUMAN DERMIS IN ONTOGENESIS].","authors":"N N Golubtzova,&nbsp;O V Vasiliyeva,&nbsp;V V Petrov,&nbsp;A G Gunin","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The goal of this study was to examine the contents of D114 and Jag-1 angiogenesis regulators in human dermis at different age periods. D114 and Jag-1 were demonstrated by indirect immunohistochemistry in skin sections of fetuses of 20-40 gestational weeks and in persons aged from birth to 85 years. D114 was studied in 150 skin samples of 72 females and 78 males, while Jag-1 was examined in 120 samples of 58 females and 62 males. It is found that the immunoreactivity was mainly expressed by the endothelial cells. Vessels, which gave a positive reaction to D114 and Jag-1, were found throughout the entire thickness of the dermis, both in fetuses, and people of all age groups. Expression of D114 in the vessels of dermal microvasculature was shown to increase from 20 weeks of gestation to 20 years. With the further age increase, the intensity of the reaction of blood vessels for D114 was decreased. Expression of Jag-1 in dermal microvessels was enhanced from 20 weeks of gestation to 85 years. The results are discussed in connection with the role of D114 and Jag-1 in angiogenesis in human dermis during ontogeny.</p>","PeriodicalId":74226,"journal":{"name":"Morfologiia (Saint Petersburg, Russia)","volume":"149 1","pages":"48-52"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34728327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[Modern clinical anatomy, problems of its teaching and development in Russia]. 现代临床解剖学在俄罗斯的教学与发展问题
Morfologiia (Saint Petersburg, Russia) Pub Date : 2016-01-01
I I Kagan
{"title":"[Modern clinical anatomy, problems of its teaching and development in Russia].","authors":"I I Kagan","doi":"","DOIUrl":"","url":null,"abstract":"","PeriodicalId":74226,"journal":{"name":"Morfologiia (Saint Petersburg, Russia)","volume":"149 1","pages":"96-9"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34729645","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
[THE INTERRELATION OF BIOLOGICAL AGE OF THE STUDENTS WITH THE INDICES OF BODY MASS, ITS COMPONENTS, AND THE CONSTITUTIONAL TYPE]. [学生生理年龄与体质指数及其组成、体质类型的相互关系]。
Morfologiia (Saint Petersburg, Russia) Pub Date : 2016-01-01
S V Mikhaylova, Yu G Kuzmichev, L I Krasnikova, N M Grinina
{"title":"[THE INTERRELATION OF BIOLOGICAL AGE OF THE STUDENTS WITH THE INDICES OF BODY MASS, ITS COMPONENTS, AND THE CONSTITUTIONAL TYPE].","authors":"S V Mikhaylova,&nbsp;Yu G Kuzmichev,&nbsp;L I Krasnikova,&nbsp;N M Grinina","doi":"","DOIUrl":"","url":null,"abstract":"<p><p>The relationship of biological age with the indices\u0000of body mass, body composition, and constitutional\u0000type was studied in 832 students aged 18–22\u0000years (302 youths and 530 girls). To determine the\u0000biological age, the method of V. P. Voytenko\u0000(1981) was used that allowed to establish\u0000functional class, rate of aging and health condition.\u0000It was found that almost half of the students had\u0000poor health associated with the accelerated rate of\u0000biological aging. A decline in the proportion of\u0000active cell mass and an increase in body fat mass\u0000was noted. In 40.6% of students with normal\u0000values of body mass index, excessive amount of\u0000body fat was determined by bioimpedance method.\u000062.2% of the students with an accelerated rate of\u0000biological aging had an increased content of a fat\u0000component in the body.</p>","PeriodicalId":74226,"journal":{"name":"Morfologiia (Saint Petersburg, Russia)","volume":"149 2","pages":"68-72"},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"36422248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
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