Molecular biology international最新文献_第3页

Expression of TPM1κ, a Novel Sarcomeric Isoform of the TPM1 Gene, in Mouse Heart and Skeletal Muscle. TPM1基因的一种新的肉瘤亚型TPM1κ在小鼠心脏和骨骼肌中的表达

Molecular biology international Pub Date : 2014-01-01 Epub Date: 2014-04-24 DOI: 10.1155/2014/896068

Syamalima Dube, Lauren Panebianco, Amr A Matoq, Henry N Chionuma, Christopher R Denz, Bernard J Poiesz, Dipak K Dube

引用次数: 8

A Synthetic Interaction between CDC20 and RAD4 in Saccharomyces cerevisiae upon UV Irradiation. 紫外辐照下酿酒酵母CDC20与RAD4的合成相互作用

Molecular biology international Pub Date : 2014-01-01 Epub Date: 2014-02-23 DOI: 10.1155/2014/519290

Bernadette Connors, Lauren Rochelle, Asela Roberts, Graham Howard

{"title":"A Synthetic Interaction between CDC20 and RAD4 in Saccharomyces cerevisiae upon UV Irradiation.","authors":"Bernadette Connors, Lauren Rochelle, Asela Roberts, Graham Howard","doi":"10.1155/2014/519290","DOIUrl":"https://doi.org/10.1155/2014/519290","url":null,"abstract":"Regulation of DNA repair can be achieved through ubiquitin-mediated degradation of transiently induced proteins. In Saccharomyces cerevisiae, Rad4 is involved in damage recognition during nucleotide excision repair (NER) and, in conjunction with Rad23, recruits other proteins to the site of damage. We identified a synthetic interaction upon UV exposure between Rad4 and Cdc20, a protein that modulates the activity of the anaphase promoting complex (APC/C), a multisubunit E3 ubiquitin ligase complex. The moderately UV sensitive Δrad4 strain became highly sensitive when cdc20-1 was present, and was rescued by overexpression of CDC20. The double mutant is also deficient in elicting RNR3-lacZ transcription upon exposure to UV irradiation or 4-NQO compared with the Δrad4 single mutant. We demonstrate that the Δrad4/cdc20-1 double mutant is defective in double strand break repair by way of a plasmid end-joining assay, indicating that Rad4 acts to ensure that damaged DNA is repaired via a Cdc20-mediated mechanism. This study is the first to present evidence that Cdc20 may play a role in the degradation of proteins involved in nucleotide excision repair. ","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":"2014 ","pages":"519290"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/519290","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32241590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Human Epidermal Growth Factor Receptor 2 (HER2) in Cancers: Overexpression and Therapeutic Implications. 人表皮生长因子受体2 (HER2)在癌症中的过度表达及其治疗意义

Molecular biology international Pub Date : 2014-01-01 Epub Date: 2014-09-07 DOI: 10.1155/2014/852748

Nida Iqbal, Naveed Iqbal

引用次数: 747

The Influence of DNA Extraction Procedure and Primer Set on the Bacterial Community Analysis by Pyrosequencing of Barcoded 16S rRNA Gene Amplicons. 通过对条形码 16S rRNA 基因扩增子进行热测序分析 DNA 提取程序和引物组对细菌群落分析的影响

Molecular biology international Pub Date : 2014-01-01 Epub Date: 2014-07-10 DOI: 10.1155/2014/548683

Ingo C Starke, Wilfried Vahjen, Robert Pieper, Jürgen Zentek

{"title":"The Influence of DNA Extraction Procedure and Primer Set on the Bacterial Community Analysis by Pyrosequencing of Barcoded 16S rRNA Gene Amplicons.","authors":"Ingo C Starke, Wilfried Vahjen, Robert Pieper, Jürgen Zentek","doi":"10.1155/2014/548683","DOIUrl":"10.1155/2014/548683","url":null,"abstract":"In this study, the effect of different DNA extraction procedures and primer sets on pyrosequencing results regarding the composition of bacterial communities in the ileum of piglets was investigated. Ileal chyme from piglets fed a diet containing different amounts of zinc oxide was used to evaluate a pyrosequencing study with barcoded 16S rRNA PCR products. Two DNA extraction methods (bead beating versus silica gel columns) and two primer sets targeting variable regions of bacterial 16S rRNA genes (8f-534r versus 968f-1401r) were considered. The SEED viewer software of the MG-RAST server was used for automated sequence analysis. A total of 5.2 × 10(5) sequences were used for analysis after processing for read length (150 bp), minimum sequence occurrence (5), and exclusion of eukaryotic and unclassified/uncultured sequences. DNA extraction procedures and primer sets differed significantly in total sequence yield. The distribution of bacterial order and main bacterial genera was influenced significantly by both parameters. However, this study has shown that the results of pyrosequencing studies using barcoded PCR amplicons of bacterial 16S rRNA genes depend on DNA extraction and primer choice, as well as on the manner of downstream sequence analysis. ","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":"2014 ","pages":"548683"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4120916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32583316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cancer stem cells accountability in progression of head and neck squamous cell carcinoma: the most recent trends! 头颈部鳞状细胞癌进展中的癌症干细胞责任:最新趋势!

Molecular biology international Pub Date : 2014-01-01 Epub Date: 2014-02-19 DOI: 10.1155/2014/375325

Samapika Routray, Neeta Mohanty

引用次数: 26

Error Rate Comparison during Polymerase Chain Reaction by DNA Polymerase. DNA聚合酶链反应错误率比较。

Molecular biology international Pub Date : 2014-01-01 Epub Date: 2014-08-17 DOI: 10.1155/2014/287430

Peter McInerney, Paul Adams, Masood Z Hadi

{"title":"Error Rate Comparison during Polymerase Chain Reaction by DNA Polymerase.","authors":"Peter McInerney, Paul Adams, Masood Z Hadi","doi":"10.1155/2014/287430","DOIUrl":"https://doi.org/10.1155/2014/287430","url":null,"abstract":"As larger-scale cloning projects become more prevalent, there is an increasing need for comparisons among high fidelity DNA polymerases used for PCR amplification. All polymerases marketed for PCR applications are tested for fidelity properties (i.e., error rate determination) by vendors, and numerous literature reports have addressed PCR enzyme fidelity. Nonetheless, it is often difficult to make direct comparisons among different enzymes due to numerous methodological and analytical differences from study to study. We have measured the error rates for 6 DNA polymerases commonly used in PCR applications, including 3 polymerases typically used for cloning applications requiring high fidelity. Error rate measurement values reported here were obtained by direct sequencing of cloned PCR products. The strategy employed here allows interrogation of error rate across a very large DNA sequence space, since 94 unique DNA targets were used as templates for PCR cloning. The six enzymes included in the study, Taq polymerase, AccuPrime-Taq High Fidelity, KOD Hot Start, cloned Pfu polymerase, Phusion Hot Start, and Pwo polymerase, we find the lowest error rates with Pfu, Phusion, and Pwo polymerases. Error rates are comparable for these 3 enzymes and are >10x lower than the error rate observed with Taq polymerase. Mutation spectra are reported, with the 3 high fidelity enzymes displaying broadly similar types of mutations. For these enzymes, transition mutations predominate, with little bias observed for type of transition. ","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":"2014 ","pages":"287430"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/287430","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32648373","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 172

The role of suppressors of cytokine signalling in human neoplasms. 细胞因子信号抑制因子在人类肿瘤中的作用。

Molecular biology international Pub Date : 2014-01-01 Epub Date: 2014-03-16 DOI: 10.1155/2014/630797

Walid Sasi, Anup K Sharma, Kefah Mokbel

引用次数: 59

MCM Paradox: Abundance of Eukaryotic Replicative Helicases and Genomic Integrity. MCM悖论:真核生物复制解旋酶的丰度和基因组完整性。

Molecular biology international Pub Date : 2014-01-01 Epub Date: 2014-10-19 DOI: 10.1155/2014/574850

Mitali Das, Sunita Singh, Satyajit Pradhan, Gopeshwar Narayan

{"title":"MCM Paradox: Abundance of Eukaryotic Replicative Helicases and Genomic Integrity.","authors":"Mitali Das, Sunita Singh, Satyajit Pradhan, Gopeshwar Narayan","doi":"10.1155/2014/574850","DOIUrl":"https://doi.org/10.1155/2014/574850","url":null,"abstract":"As a crucial component of DNA replication licensing system, minichromosome maintenance (MCM) 2-7 complex acts as the eukaryotic DNA replicative helicase. The six related MCM proteins form a heterohexamer and bind with ORC, CDC6, and Cdt1 to form the prereplication complex. Although the MCMs are well known as replicative helicases, their overabundance and distribution patterns on chromatin present a paradox called the \"MCM paradox.\" Several approaches had been taken to solve the MCM paradox and describe the purpose of excess MCMs distributed beyond the replication origins. Alternative functions of these MCMs rather than a helicase had also been proposed. This review focuses on several models and concepts generated to solve the MCM paradox coinciding with their helicase function and provides insight into the concept that excess MCMs are meant for licensing dormant origins as a backup during replication stress. Finally, we extend our view towards the effect of alteration of MCM level. Though an excess MCM constituent is needed for normal cells to withstand stress, there must be a delineation of the threshold level in normal and malignant cells. This review also outlooks the future prospects to better understand the MCM biology. ","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":"2014 ","pages":"574850"},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/574850","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32806168","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 59

The transcriptomics of secondary growth and wood formation in conifers. 针叶树二次生长和木材形成的转录组学。

Molecular biology international Pub Date : 2013-01-01 Epub Date: 2013-10-29 DOI: 10.1155/2013/974324

Ana Carvalho, Jorge Paiva, José Louzada, José Lima-Brito

{"title":"The transcriptomics of secondary growth and wood formation in conifers.","authors":"Ana Carvalho, Jorge Paiva, José Louzada, José Lima-Brito","doi":"10.1155/2013/974324","DOIUrl":"10.1155/2013/974324","url":null,"abstract":"In the last years, forestry scientists have adapted genomics and next-generation sequencing (NGS) technologies to the search for candidate genes related to the transcriptomics of secondary growth and wood formation in several tree species. Gymnosperms, in particular, the conifers, are ecologically and economically important, namely, for the production of wood and other forestry end products. Until very recently, no whole genome sequencing of a conifer genome was available. Due to the gradual improvement of the NGS technologies and inherent bioinformatics tools, two draft assemblies of the whole genomes sequence of Picea abies and Picea glauca arose in the current year. These draft genome assemblies will bring new insights about the structure, content, and evolution of the conifer genomes. Furthermore, new directions in the forestry, breeding and research of conifers will be discussed in the following. The identification of genes associated with the xylem transcriptome and the knowledge of their regulatory mechanisms will provide less time-consuming breeding cycles and a high accuracy for the selection of traits related to wood production and quality. ","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":"2013 ","pages":"974324"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3830773/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31914252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Investigation of the Association between Genetic Polymorphism of Microsomal Epoxide Hydrolase and Primary Brain Tumor Incidence. 微粒体环氧化物水解酶遗传多态性与原发性脑肿瘤发病率的相关性研究。

Molecular biology international Pub Date : 2013-01-01 Epub Date: 2013-12-16 DOI: 10.1155/2013/189237

Ali Aydin, Hatice Pinarbasi, Mustafa Gurelik

{"title":"Investigation of the Association between Genetic Polymorphism of Microsomal Epoxide Hydrolase and Primary Brain Tumor Incidence.","authors":"Ali Aydin, Hatice Pinarbasi, Mustafa Gurelik","doi":"10.1155/2013/189237","DOIUrl":"https://doi.org/10.1155/2013/189237","url":null,"abstract":"MEH IS A CRITICAL BIOTRANSFORMATION ENZYME THAT CATALYZES THE CONVERSION OF XENOBIOTIC EPOXIDE SUBSTRATES INTO MORE POLAR DIOL METABOLITES: it is also capable of inactivating a large number of structurally different molecules. Two polymorphisms affecting enzyme activity have been described in the exon 3 and 4 of the mEH gene. The hypothesis of this study is that inherent genetic susceptibility to a primary brain tumor is associated with mEH gene polymorphisms. The polymorphisms of the mEH gene were determined with PCR-RFLP techniques and 255 Turkish individuals. Our results indicate that the frequency of the mEH exon 4 polymorphism (in controls) is significantly higher than that of primary brain tumor patients (OR = 1.8, 95% CI = 1.0-3.4). This report, however, failed to demonstrate a significant association between mEH exon 3 polymorphism and primary brain tumor susceptibility in this population. Analysis of patients by both histological types of primary brain tumor and gene variants showed no association, although analysis of family history of cancer between cases and controls showed a statistically significant association (χ (2) = 7.0, P = 0.01). Our results marginally support the hypothesis that genetic susceptibility to brain tumors may be associated with mEPHX gene polymorphisms. ","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":"2013 ","pages":"189237"},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/189237","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32054417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0