Molecular biology international最新文献

Role of B Cell Development Marker CD10 in Cancer Progression and Prognosis B细胞发育标志物CD10在肿瘤进展和预后中的作用

Molecular biology international Pub Date : 2016-11-14 DOI: 10.1155/2016/4328697

D. Mishra, Sunita Singh, G. Narayan

引用次数: 41

Drosophila Enhancer of Rudimentary Homolog, ERH, Is a Binding Partner of RPS3, RPL19, and DDIT4, Suggesting a Mechanism for the Nuclear Localization of ERH 果蝇基本同源增强子ERH是RPS3、RPL19和DDIT4的结合伙伴，提示ERH核定位的一种机制

Molecular biology international Pub Date : 2016-10-18 DOI: 10.1155/2016/8371819

S. Tsubota, Anthony C Phillips

{"title":"Drosophila Enhancer of Rudimentary Homolog, ERH, Is a Binding Partner of RPS3, RPL19, and DDIT4, Suggesting a Mechanism for the Nuclear Localization of ERH","authors":"S. Tsubota, Anthony C Phillips","doi":"10.1155/2016/8371819","DOIUrl":"https://doi.org/10.1155/2016/8371819","url":null,"abstract":"The protein enhancer of rudimentary homolog, ERH, is a small, highly conserved protein that has been found in animals, plants, and protists. Genetic and biochemical interactions have implicated ERH in the regulation of pyrimidine biosynthesis, DNA replication, transcription, mRNA splicing, cellular proliferation, tumorigenesis, and the Notch signaling pathway. In vertebrates and insects, ERH is nuclearly localized; however, an examination of the ERH amino-acid sequence does not reveal any nuclear localization signals. In this paper we show that the first 24 amino acids contain sequences necessary and sufficient for nuclear localization. Through yeast two-hybrid screens, three new binding partners of ERH, RPS3, RPL19, and DDIT4, were identified. RPS3 was isolated from both human and Drosophila screens. These interactions suggest functions of ERH in cell growth, cancer, and DNA repair. The ERH sequences necessary for the interactions between ERH and RPS3 and RPL19 are mapped onto the same 24-amino-acid region in ERH which are necessary for nuclear localization, suggesting that ERH is localizing to the nucleus through binding to one of its DNA-binding partners, such as RPS3 or RPL19.","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/8371819","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64564894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Cloning, Sequencing, and the Expression of the Elusive Sarcomeric TPM4α Isoform in Humans TPM4α亚型在人体内的克隆、测序和表达

Molecular biology international Pub Date : 2016-09-15 DOI: 10.1155/2016/3105478

D. Dube, S. Dube, L. Abbott, Ruham Alshiekh-Nasany, Charles Mitschow, B. Poiesz

{"title":"Cloning, Sequencing, and the Expression of the Elusive Sarcomeric TPM4α Isoform in Humans","authors":"D. Dube, S. Dube, L. Abbott, Ruham Alshiekh-Nasany, Charles Mitschow, B. Poiesz","doi":"10.1155/2016/3105478","DOIUrl":"https://doi.org/10.1155/2016/3105478","url":null,"abstract":"In mammals, tropomyosin is encoded by four known TPM genes (TPM1, TPM2, TPM3, and TPM4) each of which can generate a number of TPM isoforms via alternative splicing and/or using alternate promoters. In humans, the sarcomeric isoform(s) of each of the TPM genes, except for the TPM4, have been known for a long time. Recently, on the basis of computational analyses of the human genome sequence, the predicted sequence of TPM4α has been posted in GenBank. We designed primer-pairs for RT-PCR and showed the expression of the transcripts of TPM4α and a novel isoform TPM4δ in human heart and skeletal muscle. qRT-PCR shows that the relative expression of TPM4α and TPM4δ is higher in human cardiac muscle. Western blot analyses using CH1 monoclonal antibodies show the absence of the expression of TPM4δ protein (~28 kDa) in human heart muscle. 2D western blot analyses with the same antibody show the expression of at least nine distinct tropomyosin molecules with a mass ~32 kD and above in adult heart. By Mass spectrometry, we determined the amino acid sequences of the extracted proteins from these spots. Spot “G” reveals the putative expression of TPM4α along with TPM1α protein in human adult heart.","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/3105478","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64319191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Molecular Characterization of Human Rotavirus from Children with Diarrhoeal Disease in Sokoto State, Nigeria 尼日利亚索科托州腹泻病儿童感染轮状病毒的分子特征

Molecular biology international Pub Date : 2016-03-09 DOI: 10.1155/2016/1876065

B. Alkali, A. Daneji, A. A. Magaji, L. S. Bilbis, F. Bande

{"title":"Molecular Characterization of Human Rotavirus from Children with Diarrhoeal Disease in Sokoto State, Nigeria","authors":"B. Alkali, A. Daneji, A. A. Magaji, L. S. Bilbis, F. Bande","doi":"10.1155/2016/1876065","DOIUrl":"https://doi.org/10.1155/2016/1876065","url":null,"abstract":"This study was conducted to detect and characterize prevalent human group A rotavirus strains from 200 diarrheic children in Sokoto, Nigeria, by ELISA, monoclonal antibody (Mab) serotyping and Reverse Transcription-Polymerase Chain Reaction (RT-PCR) techniques. Rotavirus was detected in 25.5% of the children. The G-serotypes observed in circulation were G4: 16 (59.3%), G1: 4 (14.8%), G2: 3 (11.1%), G3: 3 (11.1%), and G12: 1 (3.7%). The monoclonal antibody (Mab) serotyping detected G1 and G3 but did not detect G4 and G2 serotypes. The Mab typing of the G1 and G3 serotypes was consistent with the result of the RT-PCR. The VP4 genotypes detected were P[6] 3 (13%), P[8] 11 (47.8%), and the rare human P genotype (P[9]), found in 9 patients (39.1%). Nine strains identified with the common G and P combinations were G4 P[8] 5 (56%), G4 P[6] 1 (11%), G1 P[8] 2 (22%), and G3 P[8] 1 (11%), while seven strains with unusual combinations or rare G or P genotypes identified were G12 P[8] 1 (14%), G2 P[8] 2 (29%), and G4 P[9] 4 (57%). To our knowledge this is the first molecular study of human rotavirus and report of rare human G and P serotypes in Sokoto State.","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-03-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/1876065","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64249918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Soluble Expression and Characterization of Biologically Active Bacillus anthracis Protective Antigen in Escherichia coli 生物活性炭疽芽孢杆菌保护性抗原在大肠杆菌中的可溶性表达及特性研究

Molecular biology international Pub Date : 2016-02-07 DOI: 10.1155/2016/4732791

N. Suryanarayana, Vanlalhmuaka, Bharti Mankere, M. Verma, K. Thavachelvam, U. Tuteja

{"title":"Soluble Expression and Characterization of Biologically Active Bacillus anthracis Protective Antigen in Escherichia coli","authors":"N. Suryanarayana, Vanlalhmuaka, Bharti Mankere, M. Verma, K. Thavachelvam, U. Tuteja","doi":"10.1155/2016/4732791","DOIUrl":"https://doi.org/10.1155/2016/4732791","url":null,"abstract":"Bacillus anthracis secretory protein protective antigen (PA) is primary candidate for subunit vaccine against anthrax. Attempts to obtain large quantity of PA from Escherichia coli expression system often result in the formation of insoluble inclusion bodies. Therefore, it is always better to produce recombinant proteins in a soluble form. In the present study, we have obtained biologically active recombinant PA in small scale E. coli shake culture system using three different expression constructs. The PA gene was cloned in expression vectors bearing trc, T5, and T7 promoters and transformed into their respective E. coli hosts. The growth conditions were optimized to obtain maximum expression of PA in soluble form. The expression construct PA-pET32c in DE3-pLysS E. coli host resulted in a maximum production of soluble PA (15 mg L−1) compared to other combinations. Purified PA was subjected to trypsin digestion and binding assay with lethal factor to confirm the protein's functionality. Biological activity was confirmed by cytotoxicity assay on J774.1 cells. Balb/c mice were immunized with PA and the immunogenicity was tested by ELISA and toxin neutralization assay. This study highlights the expression of soluble and biologically active recombinant PA in larger quantity using simpler E. coli production platform.","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-02-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"64396105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

SCAR Marker for Identification and Discrimination of Commiphora wightii and C. myrrha. SCAR 标记用于识别和区分 Commiphora wightii 和 C. myrrha。

Molecular biology international Pub Date : 2016-01-01 Epub Date: 2016-03-16 DOI: 10.1155/2016/1482796

Pramod Kumar Sairkar, Anjana Sharma, N P Shukla

引用次数: 0

Total Protein Profile and Drug Resistance in Candida albicans Isolated from Clinical Samples. 临床分离的白色念珠菌的总蛋白谱和耐药性。

Molecular biology international Pub Date : 2016-01-01 Epub Date: 2016-07-11 DOI: 10.1155/2016/4982131

Kamal Uddin Zaidi, Abin Mani, Vijay Thawani, Arti Mehra

{"title":"Total Protein Profile and Drug Resistance in Candida albicans Isolated from Clinical Samples.","authors":"Kamal Uddin Zaidi, Abin Mani, Vijay Thawani, Arti Mehra","doi":"10.1155/2016/4982131","DOIUrl":"https://doi.org/10.1155/2016/4982131","url":null,"abstract":"This study was done to assess the antifungal susceptibility of clinical isolates of Candida albicans and to evaluate its total protein profile based on morphological difference on drug resistance. Hundred and twenty clinical isolates of C. albicans from various clinical specimens were tested for susceptibility against four antifungal agents, namely, fluconazole, itraconazole, amphotericin B, and ketoconazole. A significant increase of drug resistance in clinical isolates of C. albicans was observed. The study showed 50% fluconazole and itraconazole resistance at 32 μg mL(-1) with a MIC50 and MIC90 values at 34 and 47 and 36 and 49 μg mL(-1), respectively. All isolates were sensitive to amphotericin B and ketoconazole. The SDS-PAGE protein profile showed a prevalent band of ~52.5 kDa, indicating overexpression of gene in 72% strains with fluconazole resistance. Since the opportunistic infections of Candida spp. are increasing along with drug resistance, the total protein profile will help in understanding the evolutionary changes in drug resistance and also to characterize them. ","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/4982131","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34721621","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 17

Analyses of Physcomitrella patens Ankyrin Repeat Proteins by Computational Approach. 应用计算方法分析小壶菌锚蛋白重复序列。

Molecular biology international Pub Date : 2016-01-01 Epub Date: 2016-06-27 DOI: 10.1155/2016/9156735

Niaz Mahmood, Nahid Tamanna

{"title":"Analyses of Physcomitrella patens Ankyrin Repeat Proteins by Computational Approach.","authors":"Niaz Mahmood, Nahid Tamanna","doi":"10.1155/2016/9156735","DOIUrl":"https://doi.org/10.1155/2016/9156735","url":null,"abstract":"Ankyrin (ANK) repeat containing proteins are evolutionary conserved and have functions in crucial cellular processes like cell cycle regulation and signal transduction. In this study, through an entirely in silico approach using the first release of the moss genome annotation, we found that at least 54 ANK proteins are present in P. patens. Based on their differential domain composition, the identified ANK proteins were classified into nine subfamilies. Comparative analysis of the different subfamilies of ANK proteins revealed that P. patens contains almost all the known subgroups of ANK proteins found in the other angiosperm species except for the ones having the TPR domain. Phylogenetic analysis using full length protein sequences supported the subfamily classification where the members of the same subfamily almost always clustered together. Synonymous divergence (dS) and nonsynonymous divergence (dN) ratios showed positive selection for the ANK genes of P. patens which probably helped them to attain significant functional diversity during the course of evolution. Taken together, the data provided here can provide useful insights for future functional studies of the proteins from this superfamily as well as comparative studies of ANK proteins. ","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2016-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2016/9156735","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34569480","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

FOXO3a Gene Polymorphism Associated with Asthma in Indian Population 印度人群中FOXO3a基因多态性与哮喘相关

Molecular biology international Pub Date : 2015-12-09 DOI: 10.1155/2015/638515

S. Barkund, Tejas Shah, Nikhil Ambatkar, M. Gadgil, K. Joshi

引用次数: 22

Evaluation of Loop-Mediated Isothermal Amplification Assay for the Detection of Pneumocystis jirovecii in Immunocompromised Patients 环介导等温扩增法检测免疫功能低下患者肺囊虫的评价

Molecular biology international Pub Date : 2015-11-19 DOI: 10.1155/2015/819091

Preeti Singh, Sundeep Singh, B. Mirdha, R. Guleria, S. Agarwal, A. Mohan

{"title":"Evaluation of Loop-Mediated Isothermal Amplification Assay for the Detection of Pneumocystis jirovecii in Immunocompromised Patients","authors":"Preeti Singh, Sundeep Singh, B. Mirdha, R. Guleria, S. Agarwal, A. Mohan","doi":"10.1155/2015/819091","DOIUrl":"https://doi.org/10.1155/2015/819091","url":null,"abstract":"Pneumocystis pneumonia (PCP) is one of the common opportunistic infection among HIV and non-HIV immunocompromised patients. The lack of a rapid and specific diagnostic test necessitates a more reliable laboratory diagnostic test for PCP. In the present study, the loop-mediated isothermal amplification (LAMP) assay was evaluated for the detection of Pneumocystis jirovecii. 185 clinical respiratory samples, including both BALF and IS, were subjected to GMS staining, nested PCR, and LAMP assay. Of 185 respiratory samples, 12/185 (6.5%), 41/185 (22.2%), and 49/185 (26.5%) samples were positive by GMS staining, nested PCR, and LAMP assay, respectively. As compared to nested PCR, additional 8 samples were positive by LAMP assay and found to be statistically significant (p < 0.05) with the detection limit of 1 pg. Thus, the LAMP assay may serve as a better diagnostic tool for the detection of P. jirovecii with high sensitivity and specificity, less turn-around time, operational simplicity, single-step amplification, and immediate visual detection.","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-11-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/819091","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65165750","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13