Molecular biology international最新文献_第2页

Assessment of Immunotoxicity of Dextran Coated Ferrite Nanoparticles in Albino Mice 右旋糖酐包被纳米铁氧体对白化小鼠的免疫毒性评价

Molecular biology international Pub Date : 2015-10-20 DOI: 10.1155/2015/518527

S. Syama, V. Gayathri, P. Mohanan

{"title":"Assessment of Immunotoxicity of Dextran Coated Ferrite Nanoparticles in Albino Mice","authors":"S. Syama, V. Gayathri, P. Mohanan","doi":"10.1155/2015/518527","DOIUrl":"https://doi.org/10.1155/2015/518527","url":null,"abstract":"In this study, dextran coated ferrite nanoparticles (DFNPs) of size <25 nm were synthesized, characterized, and evaluated for cytotoxicity, immunotoxicity, and oxidative stress by in vitro and in vivo methods. Cytotoxicity was performed in vitro using splenocytes with different concentrations of DFNPs. Gene expression of selected cytokines (IL-1, IL-10, and TNF β) secretion by splenocytes was evaluated. Also, 100 mg of DFNPs was injected intraperitoneally to 18 albino mice for immunological stimulations. Six animals each were sacrificed at the end of 7, 14, and 21 days. Spleen was subjected to immunotoxic response and liver was analyzed for antioxidant parameters (lipid peroxidation, reduced glutathione, glutathione peroxidase, superoxide dismutase, and glutathione reductase). The results indicated that DFNPs failed to induce any immunological reactions and no significant alternation in antioxidant defense mechanism. Also, mRNA expression of the cytokines revealed an increase in IL-10 expression and subsequent decreased expression of IL-1 and TNF β. Eventually, DNA sequencing of liver actin gene revealed base alteration in nonconserved regions (10–20 bases) of all the treated groups when compared to control samples. Hence, it can be concluded that the DFNPs were nontoxic at the cellular level and nonimmunotoxic when exposed intraperitoneally to mice.","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-10-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/518527","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"65010847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Redox Changes Induced by General Anesthesia in Critically Ill Patients with Multiple Traumas. 全身麻醉对多发创伤危重患者氧化还原变化的影响。

Molecular biology international Pub Date : 2015-01-01 DOI: 10.1155/2015/238586

Marius Papurica, Alexandru Florin Rogobete, Dorel Sandesc, Raluca Dumache, Radu Nartita, Mirela Sarandan, Alina Carmen Cradigati, Loredana Luca, Corina Vernic, Ovidiu Horea Bedreag

{"title":"Redox Changes Induced by General Anesthesia in Critically Ill Patients with Multiple Traumas.","authors":"Marius Papurica, Alexandru Florin Rogobete, Dorel Sandesc, Raluca Dumache, Radu Nartita, Mirela Sarandan, Alina Carmen Cradigati, Loredana Luca, Corina Vernic, Ovidiu Horea Bedreag","doi":"10.1155/2015/238586","DOIUrl":"https://doi.org/10.1155/2015/238586","url":null,"abstract":"The critically ill polytrauma patient is a constant challenge for the trauma team due to the complexity of the complications presented. Intense inflammatory response and infections, as well as multiple organ dysfunctions, significantly increase the rate of morbidity and mortality in these patients. Moreover, due to the physiological and biochemical imbalances present in this type of patients, the bioproduction of free radicals is significantly accelerated, thus installing the oxidative stress. In the therapeutic management of such patients, multiple surgical interventions are required and therefore they are being subjected to repeated general anesthesia. In this paper, we want to present the pathophysiological implications of oxidative stress in critically ill patients with multiple traumas and the implications of general anesthesia on the redox mechanisms of the cell. We also want to summarize the antioxidant treatments able to reduce the intensity of oxidative stress by modulating the biochemical activity of some cellular mechanisms. ","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/238586","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10474005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 13

Identification of Putative Molecular Markers Associated with Root Traits in Coffea canephora Pierre ex Froehner. 鉴定与 Coffea canephora Pierre ex Froehner 根系特征相关的假定分子标记。

Molecular biology international Pub Date : 2015-01-01 Epub Date: 2015-03-03 DOI: 10.1155/2015/532386

Devaraja Achar, Mallikarjuana G Awati, M Udayakumar, T G Prasad

{"title":"Identification of Putative Molecular Markers Associated with Root Traits in Coffea canephora Pierre ex Froehner.","authors":"Devaraja Achar, Mallikarjuana G Awati, M Udayakumar, T G Prasad","doi":"10.1155/2015/532386","DOIUrl":"10.1155/2015/532386","url":null,"abstract":"Coffea canephora exhibit poor root system and are very sensitive to drought stress that affects growth and production. Deeper root system has been largely empirical as better avoidance to soil water limitation in drought condition. The present study aimed to identify molecular markers linked to high root types in Coffea canephora using molecular markers. Contrasting parents, L1 valley with low root and S.3334 with high root type, were crossed, and 134 F1 individuals were phenotyped for root and associated physiological traits (29 traits) and genotyped with 41 of the 320 RAPD and 9 of the 55 SSR polymorphic primers. Single marker analysis was deployed for detecting the association of markers linked to root associated traits by SAS software. There were 13 putative RAPD markers associated with root traits such as root length, secondary roots, root dry weight, and root to shoot ratio, in which root length associated marker OPS1850 showed high phenotypic variance of 6.86%. Two microsatellite markers linked to root length (CPCM13400) and root to shoot ratio (CM211300). Besides, 25 markers were associated with more than one trait and few of the markers were associated with positively related physiological traits and can be used in marker assisted trait selection. ","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4363682/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33173332","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Electrostatic Interactions between Complement Regulator CD46(SCR1-2) and Adenovirus Ad11/Ad21 Fiber Protein Knob. 补体调节因子CD46(SCR1-2)与腺病毒Ad11/Ad21纤维蛋白旋涡的静电相互作用。

Molecular biology international Pub Date : 2015-01-01 Epub Date: 2015-08-19 DOI: 10.1155/2015/967465

Carl Z Chen, Ronald D Gorham, Zied Gaieb, Dimitrios Morikis

引用次数: 1

Systems Medicine: The Application of Systems Biology Approaches for Modern Medical Research and Drug Development. 系统医学:系统生物学方法在现代医学研究和药物开发中的应用。

Molecular biology international Pub Date : 2015-01-01 Epub Date: 2015-08-18 DOI: 10.1155/2015/698169

Duncan Ayers, Philip J Day

{"title":"Systems Medicine: The Application of Systems Biology Approaches for Modern Medical Research and Drug Development.","authors":"Duncan Ayers, Philip J Day","doi":"10.1155/2015/698169","DOIUrl":"https://doi.org/10.1155/2015/698169","url":null,"abstract":"The exponential development of highly advanced scientific and medical research technologies throughout the past 30 years has arrived to the point where the high number of characterized molecular agents related to pathogenesis cannot be readily integrated or processed by conventional analytical approaches. Indeed, the realization that several moieties are signatures of disease has partly led to the increment of complex diseases being characterized. Scientists and clinicians can now investigate and analyse any individual dysregulations occurring within the genomic, transcriptomic, miRnomic, proteomic, and metabolomic levels thanks to currently available advanced technologies. However, there are drawbacks within this scientific brave new age in that only isolated molecular levels are individually investigated for their influence in affecting any particular health condition. Since their conception in 1992, systems biology/medicine focuses mainly on the perturbations of overall pathway kinetics for the consequent onset and/or deterioration of the investigated condition/s. Systems medicine approaches can therefore be employed for shedding light in multiple research scenarios, ultimately leading to the practical result of uncovering novel dynamic interaction networks that are critical for influencing the course of medical conditions. Consequently, systems medicine also serves to identify clinically important molecular targets for diagnostic and therapeutic measures against such a condition. ","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2015-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2015/698169","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"34164129","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 56

Primer Based Approach for PCR Amplification of High GC Content Gene: Mycobacterium Gene as a Model. 基于引物的高GC含量基因PCR扩增方法——以分枝杆菌基因为模型。

Molecular biology international Pub Date : 2014-01-01 Epub Date: 2014-03-24 DOI: 10.1155/2014/937308

Arbind Kumar, Jagdeep Kaur

引用次数: 24

An Improved Method for Soil DNA Extraction to Study the Microbial Assortment within Rhizospheric Region. 一种改进的土壤DNA提取方法研究根际微生物分类。

Molecular biology international Pub Date : 2014-01-01 Epub Date: 2014-09-15 DOI: 10.1155/2014/518960

Faria Fatima, Neelam Pathak, Smita Rastogi Verma

{"title":"An Improved Method for Soil DNA Extraction to Study the Microbial Assortment within Rhizospheric Region.","authors":"Faria Fatima, Neelam Pathak, Smita Rastogi Verma","doi":"10.1155/2014/518960","DOIUrl":"https://doi.org/10.1155/2014/518960","url":null,"abstract":"The need for identification of soil microbial community mainly depends on direct extraction of DNA from soil, a multifaceted environment that is a major pool for microbial genetic diversity. The soil DNA extraction procedures usually suffer from two major problems, namely, inappropriate rupturing of cells and contamination with humic substances. In the present study, five protocols for single type of rhizospheric soil were investigated and their comparison indicated that the inclusion of 120 mM phosphate buffered saline (PBS) for washing and mannitol in the lysis buffer allowed the processing of soil sample in minimal time with no specific equipment requirement. Furthermore, DNA purity and yield were also improved, which allowed the exploitation of genetic potential of soil microbes within soil sample thereby facilitating the amplification of metagenomic DNA. The effectiveness of methods was analyzed using random amplification of polymorphic DNA. The banding patterns revealed that both the abundance and the composition of indigenous microbial community depend on the DNA recovery method. ","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/518960","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32735191","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

Interleukin-6 c.-174G>C Polymorphism and Periodontitis in a Brazilian Population. 白细胞介素- 6c - 174g >C多态性与巴西人群牙周炎的关系

Molecular biology international Pub Date : 2014-01-01 Epub Date: 2014-12-04 DOI: 10.1155/2014/490308

Fernanda Gabriela Teixeira, Samir Andrade Mendonça, Kamilla Menezes Oliveira, Djanilson Barbosa Dos Santos, Lucas Miranda Marques, Maise Mendonça Amorim, Raquel de Souza Gestinari

{"title":"Interleukin-6 c.-174G>C Polymorphism and Periodontitis in a Brazilian Population.","authors":"Fernanda Gabriela Teixeira, Samir Andrade Mendonça, Kamilla Menezes Oliveira, Djanilson Barbosa Dos Santos, Lucas Miranda Marques, Maise Mendonça Amorim, Raquel de Souza Gestinari","doi":"10.1155/2014/490308","DOIUrl":"https://doi.org/10.1155/2014/490308","url":null,"abstract":"Aim. Periodontitis is an inflammatory disease that affects the teeth supporting structures, triggered by periodontal pathogens, and is influenced by environmental and genetic factors. Genes encoding molecules related to the immune response, such as cytokine, are the main candidates for polymorphisms analysis and may be possibly associated with this pathology. A G/C promoter polymorphism on the IL6 gene has been shown to affect basal IL-6 levels. The aim of this study was to investigate the association between the IL6 c.-174G>C polymorphism and periodontitis in individuals from Vitória da Conquista, Bahia, Brazil. Material and Methods. Three hundred and thirty individuals (134 cases, 196 controls) were genotyped for the IL6 c.-174G>C by MS-PCR technique. Concentrations of salivary IL-6 were determined by ELISA method. Results. The IL6 c.-174G>C polymorphism was associated with periodontitis when comparing the distribution of genotypes between patients with periodontitis and control subjects. The GC genotype appeared as a protective factor for periodontitis. Results showed increased levels of salivary IL-6 in periodontitis patients. Nevertheless, there was no relationship between the concentrations of IL-6 and genotypes when comparing the case and control groups. Conclusions. Our data indicate an association between IL6 c.-174G>C polymorphism and periodontitis and showed that IL-6 may be considered an important marker for periodontitis. ","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2014-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2014/490308","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32940776","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 23

mTOR Signaling in Protein Translation Regulation: Implications in Cancer Genesis and Therapeutic Interventions. 蛋白翻译调控中的mTOR信号:在癌症发生和治疗干预中的意义。

Molecular biology international Pub Date : 2014-01-01 Epub Date: 2014-11-20 DOI: 10.1155/2014/686984

Mehvish Showkat, Mushtaq A Beigh, Khurshid I Andrabi

引用次数: 173

Regulatory Variants and Disease: The E-Cadherin -160C/A SNP as an Example. 调控变异体与疾病:以E-Cadherin -160C/A SNP为例

Molecular biology international Pub Date : 2014-01-01 Epub Date: 2014-09-02 DOI: 10.1155/2014/967565

Gongcheng Li, Tiejun Pan, Dan Guo, Long-Cheng Li

引用次数: 38