Total Protein Profile and Drug Resistance in Candida albicans Isolated from Clinical Samples.

Molecular biology international Pub Date : 2016-01-01 Epub Date: 2016-07-11 DOI:10.1155/2016/4982131

Kamal Uddin Zaidi, Abin Mani, Vijay Thawani, Arti Mehra

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引用次数: 17

Abstract

This study was done to assess the antifungal susceptibility of clinical isolates of Candida albicans and to evaluate its total protein profile based on morphological difference on drug resistance. Hundred and twenty clinical isolates of C. albicans from various clinical specimens were tested for susceptibility against four antifungal agents, namely, fluconazole, itraconazole, amphotericin B, and ketoconazole. A significant increase of drug resistance in clinical isolates of C. albicans was observed. The study showed 50% fluconazole and itraconazole resistance at 32 μg mL(-1) with a MIC50 and MIC90 values at 34 and 47 and 36 and 49 μg mL(-1), respectively. All isolates were sensitive to amphotericin B and ketoconazole. The SDS-PAGE protein profile showed a prevalent band of ~52.5 kDa, indicating overexpression of gene in 72% strains with fluconazole resistance. Since the opportunistic infections of Candida spp. are increasing along with drug resistance, the total protein profile will help in understanding the evolutionary changes in drug resistance and also to characterize them.

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临床分离的白色念珠菌的总蛋白谱和耐药性。

本研究旨在评估临床分离的白色念珠菌的抗真菌敏感性，并根据其耐药性的形态差异评估其总蛋白谱。从不同临床标本中分离的120株白色念珠菌对氟康唑、伊曲康唑、两性霉素B和酮康唑4种抗真菌药物进行了药敏试验。临床分离的白色念珠菌的耐药性明显增加。结果表明，氟康唑和伊曲康唑对32 μg mL(-1)的耐药率为50%，MIC50和MIC90分别为34和47、36和49 μg mL(-1)。所有分离株均对两性霉素B和酮康唑敏感。SDS-PAGE蛋白谱显示~52.5 kDa的流行带，表明该基因在72%的氟康唑耐药菌株中过表达。由于念珠菌的机会性感染随着耐药性的增加而增加，总蛋白谱将有助于了解耐药性的进化变化并对其进行表征。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Molecular biology international

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