Molecular biology international最新文献_第4页

The RASSF1 Gene and the Opposing Effects of the RASSF1A and RASSF1C Isoforms on Cell Proliferation and Apoptosis. RASSF1基因及RASSF1A和RASSF1C亚型对细胞增殖和凋亡的相反作用

Molecular biology international Pub Date : 2013-01-01 Epub Date: 2013-11-12 DOI: 10.1155/2013/145096

Mark E Reeves, Matthew Firek, Shin-Tai Chen, Yousef Amaar

{"title":"The RASSF1 Gene and the Opposing Effects of the RASSF1A and RASSF1C Isoforms on Cell Proliferation and Apoptosis.","authors":"Mark E Reeves, Matthew Firek, Shin-Tai Chen, Yousef Amaar","doi":"10.1155/2013/145096","DOIUrl":"https://doi.org/10.1155/2013/145096","url":null,"abstract":"RASSF1A has been demonstrated to be a tumor suppressor, while RASSF1C is now emerging as a growth promoting protein in breast and lung cancer cells. To further highlight the dual functionality of the RASSF1 gene, we have compared the effects of RASSF1A and RASSF1C on cell proliferation and apoptosis in the presence of TNF- α . Overexpression of RASSF1C in breast and lung cancer cells reduced the effects of TNF- α on cell proliferation, apoptosis, and MST1/2 phosphorylation, while overexpression of RASSF1A had the opposite effect. We also assessed the expression of RASSF1A and RASSF1C in breast and lung tumor and matched normal tissues. We found that RASSF1A mRNA levels are significantly higher than RASSF1C mRNA levels in all normal breast and lung tissues examined. In addition, RASSF1A expression is significantly downregulated in 92% of breast tumors and in 53% of lung tumors. Conversely, RASSF1C was upregulated in 62% of breast tumors and in 47% of lung tumors. Together, these findings suggest that RASSF1C, unlike RASSF1A, is not a tumor suppressor but instead may play a role in stimulating survival in breast and lung cancer cells. ","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/145096","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31945969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 25

Communication and the emergence of collective behavior in living organisms: a quantum approach. 生命有机体中交流和集体行为的出现:量子方法。

Molecular biology international Pub Date : 2013-01-01 Epub Date: 2013-10-30 DOI: 10.1155/2013/987549

Marco Bischof, Emilio Del Giudice

{"title":"Communication and the emergence of collective behavior in living organisms: a quantum approach.","authors":"Marco Bischof, Emilio Del Giudice","doi":"10.1155/2013/987549","DOIUrl":"https://doi.org/10.1155/2013/987549","url":null,"abstract":"Intermolecular interactions within living organisms have been found to occur not as individual independent events but as a part of a collective array of interconnected events. The problem of the emergence of this collective dynamics and of the correlated biocommunication therefore arises. In the present paper we review the proposals given within the paradigm of modern molecular biology and those given by some holistic approaches to biology. In recent times, the collective behavior of ensembles of microscopic units (atoms/molecules) has been addressed in the conceptual framework of Quantum Field Theory. The possibility of producing physical states where all the components of the ensemble move in unison has been recognized. In such cases, electromagnetic fields trapped within the ensemble appear. In the present paper we present a scheme based on Quantum Field Theory where molecules are able to move in phase-correlated unison among them and with a self-produced electromagnetic field. Experimental corroboration of this scheme is presented. Some consequences for future biological developments are discussed. ","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/987549","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31914253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 79

GAPDH Pseudogenes and the Quantification of Feline Genomic DNA Equivalents. GAPDH假基因和猫基因组DNA等同物的定量。

Molecular biology international Pub Date : 2013-01-01 Epub Date: 2013-04-28 DOI: 10.1155/2013/587680

A Katrin Helfer-Hungerbuehler, Stefan Widmer, Regina Hofmann-Lehmann

{"title":"GAPDH Pseudogenes and the Quantification of Feline Genomic DNA Equivalents.","authors":"A Katrin Helfer-Hungerbuehler, Stefan Widmer, Regina Hofmann-Lehmann","doi":"10.1155/2013/587680","DOIUrl":"https://doi.org/10.1155/2013/587680","url":null,"abstract":"Quantitative real-time PCR (qPCR) is broadly used to detect and quantify nucleic acid targets. In order to determine cell copy number and genome equivalents, a suitable reference gene that is present in a defined number in the genome is needed, preferably as a single copy gene. For most organisms, a variable number of glyceraldehyde-3-phosphate dehydrogenase (GAPDH) pseudogenes have been reported. However, it has been suggested that a single-copy of the GAPDH pseudogene is present in the feline genome and that a GAPDH assay can therefore be used to quantify feline genomic DNA (gDNA). The aim of this study was to determine whether one or more GAPDH pseudogenes are present in the feline genome and to provide a suitable alternative qPCR system for the quantification of feline cell copy number and genome equivalents. Bioinformatics and sequencing results revealed that not just one but several closely related GAPDH-like sequences were present in the cat genome. We thus identified, developed, optimized, and validated an alternative reference gene assay using feline albumin (fALB). Our data emphasize the need for an alternative reference gene, apart from the GAPDH pseudogene, for the normalization of gDNA levels. We recommend using the fALB qPCR assay for future studies.","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/587680","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"31482361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 18

Sequence Characterization of Mitochondrial 12S rRNA Gene in Mouse Deer (Moschiola indica) for PCR-RFLP Based Species Identification. 基于PCR-RFLP的鼠鹿线粒体12S rRNA基因序列鉴定

Molecular biology international Pub Date : 2013-01-01 Epub Date: 2013-12-23 DOI: 10.1155/2013/783925

Chandra Mohan Siddappa, Mohini Saini, Asit Das, Ramesh Doreswamy, Anil K Sharma, Praveen K Gupta

{"title":"Sequence Characterization of Mitochondrial 12S rRNA Gene in Mouse Deer (Moschiola indica) for PCR-RFLP Based Species Identification.","authors":"Chandra Mohan Siddappa, Mohini Saini, Asit Das, Ramesh Doreswamy, Anil K Sharma, Praveen K Gupta","doi":"10.1155/2013/783925","DOIUrl":"https://doi.org/10.1155/2013/783925","url":null,"abstract":"Mitochondrial 12S rRNA has proven to be a useful molecular marker for better conservation and management of the endangered species. Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) of the mitochondrial 12S rRNA gene has proven to be a reliable and efficient tool for the identification of different Indian deer species of family cervidae. In the present study, mitochondrial 12S rRNA gene sequence of mouse deer (Moschiola indica) belonging to the family Tragulidae was characterized and analysed in silico for its use in species identification. Genomic DNA was isolated from the hair follicles and mitochondrial 12S rRNA gene was amplified using universal primers. PCR product was cloned and sequenced for the first time. The sequence of mouse deer showed 90.04, 90.08, 90.04, 91.2, 90.04, and 90.08% identities with sika deer, sambar, hog deer, musk deer, chital, and barking deer, respectively. Restriction mapping in Lasergene (DNAstar Inc., Madison, WI, USA) revealed that mouse deer mitochondrial 12S rRNA gene sequence can be differentiated from the other deer species in PCR-RFLP using RsaI, DdeI, BsrI, and BstSFI. With the help of predicted pattern, mouse deer can be identified using genomic DNA from a variety of biomaterials, thereby providing molecular aid in wildlife forensics and conservation of the species. ","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2013-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2013/783925","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"32054418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 10

Host-pathogen interactions of retroviruses. 逆转录病毒的宿主-病原体相互作用。

Molecular biology international Pub Date : 2012-01-01 Epub Date: 2012-10-24 DOI: 10.1155/2012/648512

Abdul A Waheed, Abraham L Brass, Suryaram Gummuluru, Gilda Tachedjian

引用次数: 2

Hippo and rassf1a Pathways: A Growing Affair. 河马和rassf1a通路:一个不断增长的事件。

Molecular biology international Pub Date : 2012-01-01 Epub Date: 2012-07-05 DOI: 10.1155/2012/307628

Francesca Fausti, Silvia Di Agostino, Andrea Sacconi, Sabrina Strano, Giovanni Blandino

引用次数: 33

Protease-Mediated Maturation of HIV: Inhibitors of Protease and the Maturation Process. 蛋白酶介导的HIV成熟:蛋白酶抑制剂和成熟过程。

Molecular biology international Pub Date : 2012-01-01 Epub Date: 2012-07-25 DOI: 10.1155/2012/604261

Catherine S Adamson

{"title":"Protease-Mediated Maturation of HIV: Inhibitors of Protease and the Maturation Process.","authors":"Catherine S Adamson","doi":"10.1155/2012/604261","DOIUrl":"https://doi.org/10.1155/2012/604261","url":null,"abstract":"Protease-mediated maturation of HIV-1 virus particles is essential for virus infectivity. Maturation occurs concomitant with immature virus particle release and is mediated by the viral protease (PR), which sequentially cleaves the Gag and Gag-Pol polyproteins into mature protein domains. Maturation triggers a second assembly event that generates a condensed conical capsid core. The capsid core organizes the viral RNA genome and viral proteins to facilitate viral replication in the next round of infection. The fundamental role of proteolytic maturation in the generation of mature infectious particles has made it an attractive target for therapeutic intervention. Development of small molecules that target the PR active site has been highly successful and nine protease inhibitors (PIs) have been approved for clinical use. This paper provides an overview of their development and clinical use together with a discussion of problems associated with drug resistance. The second-half of the paper discusses a novel class of antiretroviral drug termed maturation inhibitors, which target cleavage sites in Gag not PR itself. The paper focuses on bevirimat (BVM) the first-in-class maturation inhibitor: its mechanism of action and the implications of naturally occurring polymorphisms that confer reduced susceptibility to BVM in phase II clinical trials.","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/604261","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30830434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 32

The Role of TNPO3 in HIV-1 Replication. TNPO3在HIV-1复制中的作用

Molecular biology international Pub Date : 2012-01-01 Epub Date: 2012-07-19 DOI: 10.1155/2012/868597

Felipe Diaz-Griffero

引用次数: 20

RASSF1 Polymorphisms in Cancer. 癌症中的 RASSF1 多态性。

Molecular biology international Pub Date : 2012-01-01 Epub Date: 2012-05-31 DOI: 10.1155/2012/365213

Marilyn Gordon, Mohamed El-Kalla, Shairaz Baksh

引用次数: 0

Modulator of Apoptosis 1: A Highly Regulated RASSF1A-Interacting BH3-Like Protein. 凋亡调节因子1:一个高度调控的rassf1a相互作用bh3样蛋白。

Molecular biology international Pub Date : 2012-01-01 Epub Date: 2012-06-14 DOI: 10.1155/2012/536802

Jennifer Law, Victor C Yu, Shairaz Baksh

{"title":"Modulator of Apoptosis 1: A Highly Regulated RASSF1A-Interacting BH3-Like Protein.","authors":"Jennifer Law, Victor C Yu, Shairaz Baksh","doi":"10.1155/2012/536802","DOIUrl":"https://doi.org/10.1155/2012/536802","url":null,"abstract":"Modulator of apoptosis 1 (MOAP-1) is a BH3-like protein that plays key roles in both the intrinsic and extrinsic modes of cell death or apoptosis. MOAP-1 is part of the Ras association domain family 1A (RASSF1A)/MOAP-1 pro-apoptotic extrinsic signaling pathway that regulates apoptosis by utilizing death receptors such as tumor necrosis factor α (TNFα) or TNF-related apoptosis-inducing ligand (TRAIL) to inhibit abnormal growth. RASSF1A is a bona fide tumor suppressor gene that is epigenetically silenced by promoter-specific methylation in numerous human cancers. MOAP-1 is a downstream effector of RASSF1A that promotes Bax activation and cell death and is highly regulated during apoptosis. We speculate that MOAP-1 and RASSF1A are important elements of an \"apoptotic checkpoint\" that directly influences the outcome of cell death. The failure to regulate this pro-apoptotic pathway may result in the appearance of cancer and possibly other disorders. Although loss of RASSF1A expression is frequently observed in human cancers, it is currently unknown if MOAP-1 expression may also be affected during carcinogenesis to result in uncontrolled malignant growth. In this article, we will summarize what is known about the biological role(s) of MOAP-1 and how it functions as a downstream effector to RASSF1A.","PeriodicalId":74217,"journal":{"name":"Molecular biology international","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2012-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1155/2012/536802","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"30726423","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 20