microLife最新文献

{"title":"Grad-seq analysis of <i>Enterococcus faecalis</i> and <i>Enterococcus faecium</i> provides a global view of RNA and protein complexes in these two opportunistic pathogens.","authors":"Charlotte Michaux, Milan Gerovac, Elisabeth E Hansen, Lars Barquist, Jörg Vogel","doi":"10.1093/femsml/uqac027","DOIUrl":"10.1093/femsml/uqac027","url":null,"abstract":"<p><p><i>Enterococcus faecalis</i> and <i>Enterococcus faecium</i> are major nosocomial pathogens. Despite their relevance to public health and their role in the development of bacterial antibiotic resistance, relatively little is known about gene regulation in these species. RNA-protein complexes serve crucial functions in all cellular processes associated with gene expression, including post-transcriptional control mediated by small regulatory RNAs (sRNAs). Here, we present a new resource for the study of enterococcal RNA biology, employing the Grad-seq technique to comprehensively predict complexes formed by RNA and proteins in <i>E. faecalis</i> V583 and <i>E. faecium</i> AUS0004. Analysis of the generated global RNA and protein sedimentation profiles led to the identification of RNA-protein complexes and putative novel sRNAs. Validating our data sets, we observe well-established cellular RNA-protein complexes such as the 6S RNA-RNA polymerase complex, suggesting that 6S RNA-mediated global control of transcription is conserved in enterococci. Focusing on the largely uncharacterized RNA-binding protein KhpB, we use the RIP-seq technique to predict that KhpB interacts with sRNAs, tRNAs, and untranslated regions of mRNAs, and might be involved in the processing of specific tRNAs. Collectively, these datasets provide departure points for in-depth studies of the cellular interactome of enterococci that should facilitate functional discovery in these and related Gram-positive species. Our data are available to the community through a user-friendly Grad-seq browser that allows interactive searches of the sedimentation profiles (https://resources.helmholtz-hiri.de/gradseqef/).</p>","PeriodicalId":74189,"journal":{"name":"microLife","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117718/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9516279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A leader cell triggers end of lag phase in populations of <i>Pseudomonas fluorescens</i>.","authors":"Maxime Ardré,&nbsp;Guilhem Doulcier,&nbsp;Naama Brenner,&nbsp;Paul B Rainey","doi":"10.1093/femsml/uqac022","DOIUrl":"10.1093/femsml/uqac022","url":null,"abstract":"Abstract The relationship between the number of cells colonizing a new environment and time for resumption of growth is a subject of long-standing interest. In microbiology this is known as the “inoculum effect.” Its mechanistic basis is unclear with possible explanations ranging from the independent actions of individual cells, to collective actions of populations of cells. Here, we use a millifluidic droplet device in which the growth dynamics of hundreds of populations founded by controlled numbers of Pseudomonas fluorescens cells, ranging from a single cell, to one thousand cells, were followed in real time. Our data show that lag phase decreases with inoculum size. The decrease of average lag time and its variance across droplets, as well as lag time distribution shapes, follow predictions of extreme value theory, where the inoculum lag time is determined by the minimum value sampled from the single-cell distribution. Our experimental results show that exit from lag phase depends on strong interactions among cells, consistent with a “leader cell” triggering end of lag phase for the entire population.","PeriodicalId":74189,"journal":{"name":"microLife","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-11-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117806/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9516244","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bacterial methyltransferases: from targeting bacterial genomes to host epigenetics.","authors":"Monica Rolando, Cristina Di Silvestre, Laura Gomez-Valero, Carmen Buchrieser","doi":"10.1093/femsml/uqac014","DOIUrl":"10.1093/femsml/uqac014","url":null,"abstract":"<p><p>Methyltransferase (MTases) enzymes transfer methyl groups particularly on proteins and nucleotides, thereby participating in controlling the epigenetic information in both prokaryotes and eukaryotes. The concept of epigenetic regulation by DNA methylation has been extensively described for eukaryotes. However, recent studies have extended this concept to bacteria showing that DNA methylation can also exert epigenetic control on bacterial phenotypes. Indeed, the addition of epigenetic information to nucleotide sequences confers adaptive traits including virulence-related characteristics to bacterial cells. In eukaryotes, an additional layer of epigenetic regulation is obtained by post-translational modifications of histone proteins. Interestingly, in the last decades it was shown that bacterial MTases, besides playing an important role in epigenetic regulations at the microbe level by exerting an epigenetic control on their own gene expression, are also important players in host-microbe interactions. Indeed, secreted nucleomodulins, bacterial effectors that target the nucleus of infected cells, have been shown to directly modify the epigenetic landscape of the host. A subclass of nucleomodulins encodes MTase activities, targeting both host DNA and histone proteins, leading to important transcriptional changes in the host cell. In this review, we will focus on lysine and arginine MTases of bacteria and their hosts. The identification and characterization of these enzymes will help to fight bacterial pathogens as they may emerge as promising targets for the development of novel epigenetic inhibitors in both bacteria and the host cells they infect.</p>","PeriodicalId":74189,"journal":{"name":"microLife","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117894/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9888094","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Human milk oligosaccharides induce acute yet reversible compositional changes in the gut microbiota of conventional mice linked to a reduction of butyrate levels.","authors":"Andrea Qvortrup Holst, Harshitha Jois, Martin Frederik Laursen, Morten O A Sommer, Tine Rask Licht, Martin Iain Bahl","doi":"10.1093/femsml/uqac006","DOIUrl":"10.1093/femsml/uqac006","url":null,"abstract":"<p><p>Human Milk Oligosaccharides (HMOs) are glycans with prebiotic properties known to drive microbial selection in the infant gut, which in turn influences immune development and future health. Bifidobacteria are specialized in HMO degradation and frequently dominate the gut microbiota of breastfed infants. However, some species of <i>Bacteroidaceae</i> also degrade HMOs, which may prompt selection also of these species in the gut microbiota. To investigate to what extent specific HMOs affect the abundance of naturally occurring <i>Bacteroidaceae</i> species in a complex mammalian gut environment, we conducted a study in 40 female NMRI mice administered three structurally different HMOs, namely 6'sialyllactose (6'SL, <i>n</i> = 8), 3-fucosyllactose (3FL, <i>n</i> = 16), and Lacto-N-Tetraose (LNT, <i>n</i> = 8), through drinking water (5%). Compared to a control group receiving unsupplemented drinking water (<i>n</i> = 8), supplementation with each of the HMOs significantly increased both the absolute and relative abundance of <i>Bacteroidaceae</i> species in faecal samples and affected the overall microbial composition analyzed by 16s rRNA amplicon sequencing. The compositional differences were mainly attributed to an increase in the relative abundance of the genus <i>Phocaeicola</i> (formerly <i>Bacteroides</i>) and a concomitant decrease of the genus <i>Lacrimispora</i> (formerly <i>Clostridium</i> XIVa cluster). During a 1-week washout period performed specifically for the 3FL group, this effect was reversed. Short-chain fatty acid analysis of faecal water revealed a decrease in acetate, butyrate and isobutyrate levels in animals supplemented with 3FL, which may reflect the observed decrease in the <i>Lacrimispora</i> genus. This study highlights HMO-driven <i>Bacteroidaceae</i> selection in the gut environment, which may cause a reduction of butyrate-producing clostridia.</p>","PeriodicalId":74189,"journal":{"name":"microLife","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117735/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9516240","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hidden in plain sight: challenges in proteomics detection of small ORF-encoded polypeptides.","authors":"Igor Fijalkowski, Patrick Willems, Veronique Jonckheere, Laure Simoens, Petra Van Damme","doi":"10.1093/femsml/uqac005","DOIUrl":"10.1093/femsml/uqac005","url":null,"abstract":"<p><p>Genomic studies of bacteria have long pointed toward widespread prevalence of small open reading frames (sORFs) encoding for short proteins, <100 amino acids in length. Despite the mounting genomic evidence of their robust expression, relatively little progress has been made in their mass spectrometry-based detection and various blanket statements have been used to explain this observed discrepancy. In this study, we provide a large-scale riboproteogenomics investigation of the challenging nature of proteomic detection of such small proteins as informed by conditional translation data. A panel of physiochemical properties alongside recently developed mass spectrometry detectability metrics was interrogated to provide a comprehensive evidence-based assessment of sORF-encoded polypeptide (SEP) detectability. Moreover, a large-scale proteomics and translatomics compendium of proteins produced by <i>Salmonella</i> Typhimurium (<i>S</i>. Typhimurium), a model human pathogen, across a panel of growth conditions is presented and used in support of our <i>in silico</i> SEP detectability analysis. This integrative approach is used to provide a data-driven census of small proteins expressed by <i>S</i>. Typhimurium across growth phases and infection-relevant conditions. Taken together, our study pinpoints current limitations in proteomics-based detection of novel small proteins currently missing from bacterial genome annotations.</p>","PeriodicalId":74189,"journal":{"name":"microLife","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117744/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9521981","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"It is a matter of whether we allow microbes to enter the food chain.","authors":"","doi":"10.1093/femsml/uqac021","DOIUrl":"https://doi.org/10.1093/femsml/uqac021","url":null,"abstract":"When Martin Loessner decided as a little boy to become a scientist, he was already showing a lot of resistance. Even during Carnival—the so-called ‘Fifth Season’ in the western parts of Germany celebrated with street parades and costume balls—he gave his mum ‘a hard time finding costumes to dress him (me) up as a researcher or explorer’. Since then, Martin followed his curiosity and studied biology in Freiburg i. Br., Germany, and Michigan, USA, and eventually embarked on a PhD at the Bacteriological Institute at the Technical University in Munich, Germany. Here, he further stayed as a postdoctoral researcher, habilitated and became an Assistant Professor. During his early research years, he discovered his passion for bacteriophages and how they interact with their bacterial hosts. Especially, the function of the bacterial cell envelope in the uptake and release of bacteriophages fascinated him and he was at the forefront of research into endolysins (Loessner et al. 1995). These enzymes are encoded by the bacteriophage and activated at the end of the phage multiplication cycle. At that stage, new phage particles are assembled inside the bacterial cells and the bacteria start producing endolysins. With that, they decide their own fate: the endolysin recognizes the peptidoglycan of the bacterial envelope while its catalytic domain hydrolyses the cell wall from within. Together with a membrane pore-forming holin, endolysin activity destroys the host bacterium to release the newly produced phage particles (Loessner et al. 1997). Having found ‘a new agent that works as an antimicrobial, the next question you ask is: What about resistance?’. The answer to that question was unexpected. Since endolysins target highly conserved bonds within the cell wall (Korndörfer et al. 2006), bacteria are essentially unable to modify them, which prevents them from developing resistance. As any microbiologist can imagine, the discovery of a lack of resistance to an effective antibacterial agent can be mind-blowing. So, Martin had to withstand opposing opinions from many researchers and colleagues from the field. However, up to this day, it seems to be worth the effort; researchers have not been able to find any stable bacterial resistance mechanism to endolysins. Applying phages to improve lives","PeriodicalId":74189,"journal":{"name":"microLife","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/28/71/uqac021.PMC10117865.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9517169","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Neisseria gonorrhoeae</i>-derived outer membrane vesicles package β-lactamases to promote antibiotic resistance.","authors":"Subhash Dhital,&nbsp;Pankaj Deo,&nbsp;Manasa Bharathwaj,&nbsp;Kristy Horan,&nbsp;Joshua Nickson,&nbsp;Mohammad Azad,&nbsp;Isabella Stuart,&nbsp;Seong H Chow,&nbsp;Sachith D Gunasinghe,&nbsp;Rebecca Bamert,&nbsp;Jian Li,&nbsp;Trevor Lithgow,&nbsp;Benjamin P Howden,&nbsp;Thomas Naderer","doi":"10.1093/femsml/uqac013","DOIUrl":"https://doi.org/10.1093/femsml/uqac013","url":null,"abstract":"<p><p><i>Neisseria gonorrhoeae</i> causes the sexually transmitted disease gonorrhoea. The treatment of gonorrhoea is becoming increasingly challenging, as <i>N. gonorrhoeae</i> has developed resistance to antimicrobial agents routinely used in the clinic. Resistance to penicillin is wide-spread partly due to the acquisition of β-lactamase genes. How <i>N. gonorrhoeae</i> survives an initial exposure to β-lactams before acquiring resistance genes remains to be understood. Here, using a panel of clinical isolates of <i>N. gonorrhoeae</i> we show that the β-lactamase enzyme is packaged into outer membrane vesicles (OMVs) by strains expressing <i>bla</i>_TEM-1B or <i>bla</i>_TEM-106, which protects otherwise susceptible clinical isolates from the β-lactam drug amoxycillin. We characterized the phenotypes of these clinical isolates of <i>N. gonorrhoeae</i> and the time courses over which the cross-protection of the strains is effective. Imaging and biochemical assays suggest that OMVs promote the transfer of proteins and lipids between bacteria. Thus, <i>N. gonorrhoeae</i> strains secret antibiotic degrading enzymes via OMVs enabling survival of otherwise susceptible bacteria.</p>","PeriodicalId":74189,"journal":{"name":"microLife","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a0/fb/uqac013.PMC10117772.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9517174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Remembering Pepe Casadesús.","authors":"Francisco García-Del Portillo,&nbsp;Nara Figueroa-Bossi,&nbsp;Lionello Bossi","doi":"10.1093/femsml/uqac016","DOIUrl":"https://doi.org/10.1093/femsml/uqac016","url":null,"abstract":"On Tuesday, 2 August 2022, Josep (Pepe) Casadesús unexpectedly passed away while on vacation in his native Catalonia. Pepe was born on 11 September 1951, in his grandparents’ house, a small ‘masía’, in the municipality of Casserres, region of Catalonia, Spain. Pepe was trained as microbiologist at the ‘Estación Experimental del Zaidín-CSIC’ in Granada, Spain, working in the laboratory of José Olivares. He obtained his PhD degree in 1980 with a thesis on ‘Genetic studies in Rhizobium meliloti’. In 1983, after a 2year stay at the ‘Nitrogen Fixation Laboratory’ of Ray Dixon at the University of Sussex (to learn molecular biology), he moved to the laboratory of John Roth at the University of Utah, in Salt Lake City, for a second postdoctoral period. John Roth’s lab had pioneered the use of Salmonella enterica (serovar Typhimurium) as a model organism for bacterial genetic studies. The charm and power of Salmonella genetics combined with the intellectual liveness of the lab would have an ever-lasting impact on Pepe’s scientific life. Not only did he adopt Salmonella as a model system for his subsequent studies, but he was also strongly inspired by the spirit and practices of Roth’s lab when he started his own group at the University of Seville in 1985. He never imposed experiments on his students, but preferred experimental strategies to take shape during open and friendly discussions, only occasionally animated by his persuasive skills. He returned to Utah as a visiting professor for two extended periods of time, in 1988 and in 2000. Besides enjoying the company of some faculty at the Biology Department, he loved the magnificent decor of Salt Lake City mountains. He especially liked taking walks in Red Butte Canyon, at the eastern edge of the University campus, where colour changes in the fall can overwhelm your senses (Fig. 1). After two other stays as visiting professor at the Biozentrum, Universität Basel (Switzerland) and at the Università degli Studi di Sassari (Sardinia, Italy), in 1999, Pepe became full Professor of Genetics at the University of Seville, where he stayed for the rest of his career. He received many distinctions related to his academic activity, but undoubtedly what was most rewarding for him was the appreciation and respect he received from his former students and alumni. He trained many researchers in his lab and his lectures inspired many students to undertake a career in science. Pepe was the editor of several journals like PLoS Genetics (from 2011) and was the ambassador of the American Society for Microbiology in Europe. In 2018, he was elected fellow of the American Academy of Microbiology and in 2019 of the European Academy for Microbiology. He did extensive work as reviewer of papers and grant proposals for institutions in the USA, IberoAmerica, and the European Commission. In his nearly 40 years of activity, Pepe’s research covered various aspects of Salmonella biology. Early on, he became fascinated by the mechanisms ","PeriodicalId":74189,"journal":{"name":"microLife","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/79/bc/uqac016.PMC10117750.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9516241","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reviving the view: evidence that macromolecule synthesis fuels bacterial spore germination.","authors":"Bing Zhou,&nbsp;Sima Alon,&nbsp;Lei Rao,&nbsp;Lior Sinai,&nbsp;Sigal Ben-Yehuda","doi":"10.1093/femsml/uqac004","DOIUrl":"https://doi.org/10.1093/femsml/uqac004","url":null,"abstract":"<p><p>The Gram positive bacterium <i>Bacillus subtilis</i> and its relatives are capable of forming a durable dormant long-lasting spore. Although spores can remain dormant for years, they possess the remarkable capacity to rapidly resume life and convert into actively growing cells. This cellular transition initiates with a most enigmatic irreversible event, termed germination, lasting only for a few minutes. Germination is typified by a morphological conversion that culminates in loss of spore resilient properties. Yet, the molecular events occurring during this brief critical phase are largely unknown. The current widely accepted view considers germination to occur without the need for any macromolecule synthesis; however, accumulating data from our laboratory and others, highlighted here, provide evidence that both transcription and translation occur during germination and are required for its execution. We further underline numerous overlooked studies, conducted mainly during the 1960s-1970s, reinforcing this notion. We propose to revisit the fascinating process of spore germination and redefine it as a pathway involving macromolecule synthesis. We expect our perspective to shed new light on the awakening process of a variety of spore-forming environmental, commensal, and pathogenic bacteria and possibly be applicable to additional organisms displaying a quiescent life form.</p>","PeriodicalId":74189,"journal":{"name":"microLife","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117790/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9517173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Akirin/Subolesin regulatory mechanisms at host/tick-pathogen interactions.","authors":"José de la Fuente,&nbsp;Sara Artigas-Jerónimo,&nbsp;Margarita Villar","doi":"10.1093/femsml/uqab012","DOIUrl":"https://doi.org/10.1093/femsml/uqab012","url":null,"abstract":"<p><p>Ticks and tick-borne pathogens such as <i>Anaplasma phagocytophilum</i> affect human and animal health worldwide and thus the characterization of host/tick-pathogen interactions is important for the control of tick-borne diseases. The vertebrate regulatory proteins Akirins and its tick ortholog, Subolesin, are conserved throughout the metazoan and involved in the regulation of different biological processes such as immune response to pathogen infection. Akirin/Subolesin have a key role in host/tick-pathogen interactions and exert its regulatory function primarily through interacting proteins such as transcription factors, chromatin remodelers and RNA-associated proteins. Recent results have provided evidence of <i>akirin</i>/<i>subolesin</i> genetic interactions and the interaction of Akirin/Subolesin with histones, thus suggesting a role in direct chromatin remodeling. Finally, and still to be proven, some models suggest the possibility of direct Akirin/Subolesin protein interactions with DNA. Future research should advance the characterization of Akirin/Subolesin interactome and its functional role at the host/tick-pathogen interface. These results have implications for translational biotechnology and medicine for the development of new effective interventions for the control of ticks and tick-borne diseases.</p>","PeriodicalId":74189,"journal":{"name":"microLife","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/3f/81/uqab012.PMC10117763.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9516236","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}