Journal of clinical & experimental immunology最新文献

{"title":"Delving into Emotional Wounds, Addressing Them as Issues to be Solved, as One of\u0000the Reasons We Choose to be Incarnated: with Personal Research into their Origins","authors":"","doi":"10.33140/jcei.05.02.06","DOIUrl":"https://doi.org/10.33140/jcei.05.02.06","url":null,"abstract":"As we all begin our lives by walking on to the stage in Act One/Scene\u0000One; and into the kind of Drama that’s usually entitled on the lines of\u0000‘My Life, ‘ I’m going to begin with something that David Lorimar\u0000(one of the founders of the Scientific & Medical Network) once\u0000said: that the Anecdotal has as much worth as any other evidence,\u0000in scientific research. I’m not sure who first said: “Nothing‘s ever\u0000happened, until it‘s happened to you,” but they got that right!","PeriodicalId":73657,"journal":{"name":"Journal of clinical & experimental immunology","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"82103939","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Autoimmune Disease, Deceptional vs Logical Matrices & Informational Fidelity","authors":"","doi":"10.33140/jcei.05.02.04","DOIUrl":"https://doi.org/10.33140/jcei.05.02.04","url":null,"abstract":"The continuous interplay between concreteness & randomness, the incessant interchange of conceivable, perceivable,\u0000comprehensible with the unknown, the mysterious, the abstruse & the consequential fractals from which infinite geometrical\u0000nexuses of bonds, forms of connections, pathways & associations appear as mazy combinations in a single cluster of seconds,\u0000with the true values alongside the false values to get involved & entangled to alterate, exchange perpetually their bipolar products\u0000to emerged matrices of obscure data, in which their noisy action & omnidirectional behavior of the reverberating echo of the\u0000abstract result confuses, bias & warps into one the physio-logical with the abnormal, the real with the mirroring, the tangible\u0000with the imaginery, the rational with the aberrant, the conscious with the subconscious, the psyche with the somatic, developing\u0000as a result an unconscious field of causative potentiallity, a kymatic burst of unexplained interactions & interpretations, which\u0000swirl the embedded hidden elements of distortion & delusion, in the end this horrorous deceptive complicated morphoma comes\u0000as the ultimate form of abyssal functioning against the humble simplicity and substract expression of the luminous linear true\u0000path of taintless logic.","PeriodicalId":73657,"journal":{"name":"Journal of clinical & experimental immunology","volume":"4 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84611422","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational Analysis for Prediction of Multi Epitopes Vaccine against Blue\u0000Tongue Virus Serotype 4 from VP5 and VP7 Proteins","authors":"","doi":"10.33140/jcei.05.02.03","DOIUrl":"https://doi.org/10.33140/jcei.05.02.03","url":null,"abstract":"Blue Tongue Disease (BTD) is a non-contagious insect transmitted disease of ruminants caused by double stranded RNA virus.\u0000This study aimed to predict an effective multi-epitopes vaccine against BTD from VP5 and VP7 as immunogenic proteins using\u0000immunoinformatic tools. The VP5 and VP7 proteins sequences were retrieved from GenBank of National Center for Biotechnology\u0000Information (NCBI). The sequences of each protein were aligned for conservancy using Bioedit software. Immune Epitope\u0000Database (IEDB) analysis resources were used to predict B and T cell epitopes. The proposed MHC-1 epitopes of both proteins\u0000were further subjected to molecular docking to show minimum binding energy of each epitopes. In our results, two epitopes\u0000(235-SEEV-235 and 85-PDPLSP-90) from VP5 and two epitopes (79-PISPDYTQ-86 and 297-PIFPPN-302) from VP7 were\u0000proposed as B cell epitopes since they were shown to be linear, surface accessible and antigenic epitopes. For T cells, MHC-1\u0000binding prediction tools showed multiple epitopes strongly interacted with BoLA alleles from both VP5 and VP7. Among them\u0000three epitopes, (257-KLKKVINAL-265, 487-QMHILRGPL-495 and 350-VMMRFKIPR-358) fromVP5 protein and four epitopes\u0000(86-QHMATIGVL-94, 315-TLADVYTVL-323, 17-TLQEARIVL-25 and 10-TVMRACATL-18) from VP7 protein interacted\u0000with the highest number of alleles and demonstrated best binding affinity to MHC-1 alleles. Thus were proposed as a vaccine\u0000candidate from VP5 and VP7 proteins. All the epitopes from VP5 and VP7 that interacted with MHC-1 alleles when subjected\u0000to molecular docking against the sheep b_microglobulin alleles demonstrated biologically significant higher binding affinity\u0000which expressed by their lower global and attractive energy. In conclusion, eleven epitopes were predicted as promising vaccine\u0000candidates against BTD from the VP5 and VP7 immunogenic proteins. These epitopes require to be validated experimentally\u0000through in vitro and in vivo studies.","PeriodicalId":73657,"journal":{"name":"Journal of clinical & experimental immunology","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84205637","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computational Prediction of Multi-Epitopes Vaccine from Envelope E Protein\u0000against Louping Ill Virus via Reverse Vaccinology","authors":"","doi":"10.33140/jcei.05.02.02","DOIUrl":"https://doi.org/10.33140/jcei.05.02.02","url":null,"abstract":"Louping ill disease is a zoonotic viral disease caused by louping ill virus in the genus Flavivirus. It belongs to the tick-borne\u0000flavivirus that is a part of the tick-borne encephalitis virus complex.The envelope E protein of louping ill virus is the major\u0000structural protein that plays an important role in membrane binding and inducing a protective immune response.The aim\u0000of the present study was to design multi epitopes vaccine from the envelope E glycoprotein against louping ill virus using\u0000immunoinformatic tools that elicited humoral and cellular immunity. Eighteen envelope E protein sequences were retrieved\u0000from NCBI and subjected to various immunoinformatics tools from IEDB to assess their conservancy, surface accessibility\u0000and antigenicity as promising epitopes against B cells. The binding affinity of the conserved predicted epitopes was analyzed\u0000against MHC-I and MHC-II alleles of the T cells. The predicted epitopes were further assessed for their population coverage.\u0000For B-cell 25, 18 and 12 epitopes were predicted as linear conserved epitopes, surface accessibility and antigenic respectively.\u0000However, nine epitopes overlapped all the B cell prediction tools. Among them three epitopes (205-TAEHLP-210,336-KPCR-339\u0000and 349-SPDV-352) were proposed as B cell epitopes. For T cell, 75 epitopes were found to interact with MHC-I alleles. The\u0000epitopes 130-YVYDANKV-138and356-MLITPNPTI-364 were proposed as a peptide vaccine since they interacted with the highest\u0000number of MHC-1 alleles.Moreover a total of 195core epitopes were found to interact with MHC-II alleles. The core epitopes\u0000130-YVYDANKV-138, 219-WFNDLALPW-227, 415-VIGEHAWDF-423 and 462-VALAWLGLN-470 interacted with higher\u0000number of MHC-II alleles and proposed as vaccine since they demonstrated high affinity to MHC-II alleles.The population\u0000coverage epitopes set for MHC-I and MHC-II alleles was 74.69% and 99.98%, respectively. While the epitopes set for all T cell,\u0000proposed epitopes was 100%. Nine epitopes were predicted eliciting B and T cells and proposed as vaccine candidates against\u0000louping ill virus. However, these proposed epitopes require clinical trials studies to ensure their efficacy as vaccine candidates.","PeriodicalId":73657,"journal":{"name":"Journal of clinical & experimental immunology","volume":"24 1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91294277","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Silico Prediction of Multi-Epitopes Vaccine from the Fusion F Protein Against\u0000Respiratory Syncytial Virus","authors":"","doi":"10.33140/jcei.05.02.01","DOIUrl":"https://doi.org/10.33140/jcei.05.02.01","url":null,"abstract":"Respiratory Syncytial Virus (RSV) is the major cause of the lower respiratory tract illness (RTI) in the elderly and in\u0000immunocompromised patients and children under 5 years of age. The disease causes deaths of approximately 500 infants each\u0000year. Conventional vaccine against the disease demonstrated immunological pitfalls to enhance T-helper responses and developed\u0000non-neutralising antibodies. This study aimed to predict epitopes from the fusion F protein of SRV that elicit the immune system\u0000and acted as safer efficacious vaccine. A total of 199 strains of RSV were retrieved from the NCBI database. The immune epitope\u0000database analysis resources (IEDB) were used for epitopes prediction against B and T cells. The population coverage was also\u0000calculated for the proposed epitopes against the whole world. Only two epitopes (441-YVSNK-445 and 440-DYVS-443) successfully\u0000passed all B cell prediction tools and demonstrated higher score in Emini and Kolaskar and tongaonker software. Thus were\u0000proposed as B cells epitopes. For T cells, a total of 177 epitopes were found to interact with MHC-I alleles. Among them four\u0000epitopes (53-YTSVITIEL-61; 250-YMLTNSELL-258, 198-YIDKQLLPI-206, and 450-VSVGNTLYY-458) were proposed since they\u0000interacted with the highest number of alleles and the best binding affinity to MHC-1 alleles. Moreover, a total of 397 core epitopes\u0000were found to interact with MHC-П alleles. However, the best four core proposed epitopes that interacted with higher number of\u0000MHC-II alleles were 217-IETVIEFQQ-226; 250-YMLTNSELL-258; 477-FYDPLVFPS-485 and 505-FIRKSDELL-513. Strikingly\u0000the epitope 250-YMLTNSELL-258 successfully interacted with both MHC-1and MHC-П alleles. The population coverage was\u000048.61% and 99.64% for MHC-I and MHC-II epitopes, respectively, and 100% for all T cells epitopes. Taken together ten epitopes\u0000successfully proposed as vaccine candidate against RSV. In vivo and in vitro clinical trials studies are required to elucidate the\u0000effectiveness of these epitopes as vaccine.","PeriodicalId":73657,"journal":{"name":"Journal of clinical & experimental immunology","volume":"40 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87095165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Plasmid Profiling of Antibiotic Resistant Organisms Isolated From Hospital Effluents\u0000Discharged Into Nworie River Imo State","authors":"","doi":"10.33140/jcei.05.01.05","DOIUrl":"https://doi.org/10.33140/jcei.05.01.05","url":null,"abstract":"The emergence of multiple antibiotics resistant in bacteria and the indiscriminate use of antibiotics contribute to the dissemination\u0000of resistant pathogen in the environment. Hospital effluents are potential sources of antibiotic resistant bacteria, which if released\u0000into the rivers leads to the contamination of the water by the resistant strains which are potential threat to human health as\u0000they might have direct access to man or transported from sea animals to man through food. Plasmids are major mechanism for\u0000the spread of antibiotic resistant gene in bacteria population. Plasmid profiling is one of the methods used to determine and\u0000characterize antibiotic resistance traits in bacteria. In this study, Samples were collected using sterile sample bottles at three\u0000different locations of Nworie River (Two Federal Medical Center and the third behind Umezuruike hospital) in Imo State. A\u0000total of eighteen isolates were screened for antibiotic susceptibility. The isolates were tested against ten (10) different antibiotics\u0000using the disc diffusion method. Eight (8) isolates were found to be resistant to at least five antibiotics. While the plasmid DNA\u0000were extracted using the TENS extraction method and separated by agarose gel electrophoresis. Four of the resistant strains\u0000had plasmid DNA.","PeriodicalId":73657,"journal":{"name":"Journal of clinical & experimental immunology","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83134361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunoglobulin Isotypes, C3 – C4 Complement Components, Absolute Eosinophils\u0000and Allergen Specific IgE as Biomarkers Correlated to Preschool Wheeze Children","authors":"PROF. Abdulla, M. El-Mansoury, Syed Abdu-Rahman, Prof. N. G. MORGAN”, 7. Dean","doi":"10.33140/jcei.05.01.04","DOIUrl":"https://doi.org/10.33140/jcei.05.01.04","url":null,"abstract":"Wheeze chest is a common problem among preschool children, and represents a common disorder characterized by airways\u0000obstruction. Almost half of children manifest wheeze chest symptoms before the age of six. Their parents report at least one\u0000attack within this period. This study aimed to assess the relationship between the immunoglobulin (IgA, IgM, IgG, and IgE),\u0000complement, and the level of eosinophils with children’s wheeze chest. Moreover, the level of IgE with the numbers of the attacks\u0000per year, age, family history, and eosinophilic count.\u0000A total number of a randomized (n=73) preschool children were involved in this study, divided into 2 groups, (n=52) patients\u0000with recurrent wheezy chest attacks and (n=21) controls. Samples were analyzed for immunoglobulin isotypes; complement C3,\u0000C4, Eosinophils, and specific IgE allergen test.\u0000Number of attacks were strongly correlated with IgE with a P-value of (P=0.001), as well as the manifestation with a P-value\u0000of (P=0.002), while age was weakly correlated with IgE with a P-value of (P=0.005). The other variables in this study were\u0000found to be insignificant, correlating with IgE.\u0000Regarding specific allergens, 20 different types were evaluated in patients who had elevated levels of IgE, and the findings\u0000were that all patients were not only allergic to 1 type but unlikely to many. Most definitely the majority of the cases shared\u0000Dermatophagoides Peter (8 patients) and Dermatophagoidesf ariaeni (7 patients) allergens and so the list goes descending in\u0000the number with 2 allergens were not found, Cladosporium herbarum and Penicillium notatum.","PeriodicalId":73657,"journal":{"name":"Journal of clinical & experimental immunology","volume":"303 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"77767095","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Side Effect Driven Conversion to Belatacept for Kidney Transplant Recipients in a Clinical Setting","authors":"","doi":"10.33140/jcei.05.01.03","DOIUrl":"https://doi.org/10.33140/jcei.05.01.03","url":null,"abstract":"Maintenance immunosuppression after kidney transplantation is critical to graft and patient survival. However, the optimal\u0000immunosuppressive medication may differ for patients based on adverse effects. Here we report one-year outcomes of 73 kidney\u0000transplant patients converted from tacrolimus to belatacept because of adverse effects at least 90 days after transplant.","PeriodicalId":73657,"journal":{"name":"Journal of clinical & experimental immunology","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-02-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"84374918","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antitumor Immunity as Determining Factor for Prevention of Post-Surgery Cancer Recurrence","authors":"","doi":"10.33140/jcei.05.01.01","DOIUrl":"https://doi.org/10.33140/jcei.05.01.01","url":null,"abstract":"Cancer surgery is the oldest modality in cancer management and remains an effective cancer treatment that excises visible\u0000tumor and can result in long-lasting clinical cure. Yet the mechanism for the ability of surgery to cure cancer is not understood.\u0000The conventional thinking is that surgery effectively excises all tumor before it spreads and that results in cure. However, with\u0000more recent studies to detect circulating tumor cells that can still persist after tumor excision, it is now increasingly clear that\u0000early tumor spread before clinical detection is a common process of cancer development. Then the important paradoxical\u0000question is how does a local therapy such as surgical excision cure a systemic disease like cancer? We hypothesize that the\u0000interaction between tumor metastasis and antitumor immunity answers this question. The model, which we call “post-surgery\u0000tumor recurrence window model”, suggests that establishment of cancer metastases has an L –shaped kinetics following removal\u0000of the primary tumor. Similarly, pre-surgery concomitant antitumor immunity will also decay following surgery due to lack of\u0000antigen stimulation. Whether a new metastasis can establish is determined by the balance between these two processes. Here\u0000we present animal study evidence to support this model. We also present examples of how this model may be applied to predict\u0000post-surgery prognosis in individual cancer patients.","PeriodicalId":73657,"journal":{"name":"Journal of clinical & experimental immunology","volume":"24 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2020-01-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74204675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Can cinnamon spice down autoimmune diseases?","authors":"Swarupa Pahan,&nbsp;Kalipada Pahan","doi":"10.33140/jcei.05.06.01","DOIUrl":"https://doi.org/10.33140/jcei.05.06.01","url":null,"abstract":"<p><p>Autoimmune diseases are one of the dreadful group of human diseases that have always been of keen interest to researchers. Due to complex and broad-spectrum nature, scientists are not yet able to pinpoint the pathogenesis of and delineate effective therapy against this group of diseases. However, it is becoming clear that a decrease in number and function of T regulatory cells (Treg), an increase in autoreactive Th1/Th17 cells and associated immunomodulation and inflammation participate in the pathogenesis of many autoimmune diseases. Cinnamon (<i>Cinnamonum verum</i> or <i>Cinnamonum cassia</i>) is a widely used natural spice and flavoring ingredient and its metabolite sodium benzoate (NaB) is a food-additive and FDA-approved drug against nonketotic hyperglycinemia (NKH) and urea cycle disorders (UCD). Recent studies indicate that cinnamon either in powder or extract form and NaB are capable of modulating different autoimmune pathways as well as protecting animals from different autoimmune disorders. Here, we have made an honest attempt to delineate such pieces of evidence with available anti-autoimmune mechanisms and analyze whether cinnamon supplements could be used to control the fury of autoimmune disorders.</p>","PeriodicalId":73657,"journal":{"name":"Journal of clinical & experimental immunology","volume":"5 6","pages":"252-258"},"PeriodicalIF":0.0,"publicationDate":"2020-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7720887/pdf/nihms-1649509.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38352656","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}