Journal of cancer science and clinical therapeutics最新文献_第3页

BRCA2 Status Alters the Effect of the P53 Reactivator HO-3867 in Ovarian Cancer Cells. BRCA2状态改变P53再激活物HO-3867在卵巢癌细胞中的作用

Journal of cancer science and clinical therapeutics Pub Date : 2023-01-01 Epub Date: 2023-04-27 DOI: 10.26502/jcsct.5079197

Eric J Devor, Ariane E Thomas, Brandon M Schickling

{"title":"BRCA2 Status Alters the Effect of the P53 Reactivator HO-3867 in Ovarian Cancer Cells.","authors":"Eric J Devor, Ariane E Thomas, Brandon M Schickling","doi":"10.26502/jcsct.5079197","DOIUrl":"10.26502/jcsct.5079197","url":null,"abstract":"The vast majority of ovarian cancers have a TP53 mutation. Among these, a substantial proportion also have a BRCA1 and/or a BRCA2 mutation. Given a rising interest in the therapeutic use of p53 reactivating agents, we assessed the effect that such BRCA mutants would have on the action of a p53 reactivator. As an initial tool to examine the effect of a BRCA mutation on the action of a p53 reactivator we chose to utilize a naturally occurring experimental model. The high grade serous ovarian cancer cell lines PEO1 and PEO4 were established from the same patient. Both cell lines have a missense TP53 mutation, G244D. However, PEO1 cells also have a nonsense BRCA2 mutation, Y1655ter, which is cancelled out by a second mutation, Y1655Y, that renders PEO4 cells BRCA2 wild-type. This makes these cell lines an ideal experimental platform to begin to assess the effect of a BRCA mutation on the action of a p53 reactivator. Both PEO1 and PEO4 cells were treated with a p53 reactivator, the synthetic curcumin analog HO-3867. The effect of treatment was assessed through quantitative PCR (qPCR) assays of fourteen known p53 target loci, including p53 itself. In all cases there was a definite difference between treated and untreated cells relative to their BRCA2 status. While these results are preliminary, the fact that BRCA2 status influences the effect of a p53 reactivator on numerous target loci suggests that this relationship should be further investigated and that, in future, the BRCA status of ovarian tumors containing missense TP53 mutations should be considered when opting for the therapeutic use of a p53 reactivator.","PeriodicalId":73634,"journal":{"name":"Journal of cancer science and clinical therapeutics","volume":"1 1","pages":"86-92"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11905286/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69348589","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Personalized Real-time Model of Drug Resistance in Gynecological Cancers: Not to Leave CAF Unturned 妇科癌症耐药的个性化实时模型:不放过CAF

Journal of cancer science and clinical therapeutics Pub Date : 2023-01-01 DOI: 10.26502/jcsct.5079208

Pradip De, Raed Sulaiman, Kris Gaster, Nandini Dey

{"title":"A Personalized Real-time Model of Drug Resistance in Gynecological Cancers: Not to Leave CAF Unturned","authors":"Pradip De, Raed Sulaiman, Kris Gaster, Nandini Dey","doi":"10.26502/jcsct.5079208","DOIUrl":"https://doi.org/10.26502/jcsct.5079208","url":null,"abstract":"Evidence suggests the involvement of cancer-associated fibroblasts (CAFs) in developing treatment resistance in various solid tumors. These findings emphasize the importance of understanding the biology and function of CAFs in the context of therapy resistance to develop improved treatment strategies for these solid tumors. The function of CAFs in promoting therapy resistance is organ-type specific. In the published literature, we observed an organ-type-specific imbalance regarding CAF’s role in endometrial neoplasms. Here we present a commentary on the innovative and promising approach to studying endometrial and ovarian CAFs in therapy resistance. Our personalized real-time model allows a more comprehensive understanding of CAFs' involvement in developing resistance to specific drugs or combinations. By adopting a tumor-tumor microenvironment (TME) holistic approach, we recognized the importance of studying tumor cells and the surrounding TME to understand disease progression better. Our CAF-based Two-Cell Hybrid Co-Culture model established using CAFs from resected tumor tissue from patients with gynecological cancers provides a relevant and patient-specific context for testing drug resistance. This personalized real-time model has the potential to shed light on the mechanisms by which CAFs contribute to therapy resistance. By studying the interactions between tumor cells and CAFs in this model, one can gain valuable insights into the role of CAFs in driving resistance and identify potential therapeutic targets to overcome it.","PeriodicalId":73634,"journal":{"name":"Journal of cancer science and clinical therapeutics","volume":"9 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136258907","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Xenotransplantation of Human Adipose Tissue in SCID Mice: a Model to Study Proximal Interactions between Adipose Tissue and Tumors 人脂肪组织在SCID小鼠体内的异种移植:研究脂肪组织与肿瘤近端相互作用的模型

Journal of cancer science and clinical therapeutics Pub Date : 2023-01-01 DOI: 10.26502/jcsct.5079191

Beaumel S, G. A, Duong Mn, Jordheim Lp, Delay E, Valet P, Perrial E, Dumontet C

引用次数: 0

Real World Experience with Osteosarcoma from a Tertiary Cancer Centre in India 来自印度三级癌症中心骨肉瘤的真实世界经验

Journal of cancer science and clinical therapeutics Pub Date : 2023-01-01 DOI: 10.26502/jcsct.5079198

P. Goyal, S. Joga, R. Tripathi, Arpit Jain, M. Sharma, Varun Goel, V. Talwar, U. Batra, S. Pasricha, Dinesh Chandra Doval

引用次数: 0

The PERK/Akt Pathway Mediates Apoptosis Resistance to ER Ca2+ Stress in LNCaP Prostate Cancer Cells PERK/Akt通路介导LNCaP前列腺癌细胞对ER Ca2+应激的凋亡抵抗

Journal of cancer science and clinical therapeutics Pub Date : 2023-01-01 DOI: 10.26502/jcsct.5079199

Charlotte Dubois, Dheeraj Kannancheri Puthooru, F. Vanden Abeele

引用次数: 0

5G Radiofrequency Radiation Caused the Microwave Syndrome in a Family Living Close to the Base Stations 5G射频辐射对基站附近家庭微波综合征的影响

Journal of cancer science and clinical therapeutics Pub Date : 2023-01-01 DOI: 10.26502/jcsct.5079203

Mona Nilsson, L. Hardell

{"title":"5G Radiofrequency Radiation Caused the Microwave Syndrome in a Family Living Close to the Base Stations","authors":"Mona Nilsson, L. Hardell","doi":"10.26502/jcsct.5079203","DOIUrl":"https://doi.org/10.26502/jcsct.5079203","url":null,"abstract":"The fifth generation, 5G, for wireless communication has been implemented in various countries since 2019. The deployment in Sweden started in 2020 where the used frequencies for 5G in cities are around 3.5 GHz. Recently, we published three case reports on persons that developed the microwave syndrome within short after the installation of 5G base stations close to their dwellings. The health symptoms were attributed to high levels of radiofrequency (RF) radiation measured in their apartments. In this article we examine a family of three persons living at distances of about 50 and 70 meters to two 5G base stations. The base stations are located on the top of two 6-floor buildings and the antennas are directed towards the family’s apartment on the 4 th floor on the opposite side of the street. Measurements in the apartment were made 10 times at every place, each measurement during 1 minute. Highest levels were measured close to the two windows in the master bed room varying from 320 000 to 1 200 000 μW/m 2 . High levels were also found at the window of the son’s room, 121 000 to 490 000 μW/m 2 , and the daughter’s room 34 800 to 166 000 μW/m 2 . Somewhat lower levels were found in the beds at the place of the pillow for all family members. Lowest levels were measured in the kitchen on the opposite side of the apartment, 710 to 3 260 μW/m 2 . Health problems were assessed by using a structured questionnaire similar to our previous studies. The family members reported symptoms included in the microwave syndrome to varying self-estimated degrees. The daughter had the most severe health issues, for example sleeping problems, headache, concentration and memory problems, skin disorders, irregular heartbeat, light sensitivity, anxiety and panic attacks These results are in line with previous case studies showing that deployment of 5G cannot be made without risks to human health, especially for those living or working in the proximity of the base stations.","PeriodicalId":73634,"journal":{"name":"Journal of cancer science and clinical therapeutics","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69348676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Epidemiology, Treatment, and Evolution of Glioblastoma in a Low-Income Country: Moroccan Experience 低收入国家胶质母细胞瘤的流行病学、治疗和进化:摩洛哥的经验

Journal of cancer science and clinical therapeutics Pub Date : 2023-01-01 DOI: 10.26502/jcsct.5079187

A. Naim, Hamza ouazzani, Soumya Rafii, A. Azhari, Abdellah Badou

引用次数: 0

Regulation of TGF-β1-Smad-1-7 signaling to Inhibit Epithelial Mesenchymal Transition by Repurposed Anti-Fibrotic Drug Pirfenidone can Attenuate Lung Cancer Progression 抗纤维化药物吡非尼酮调节TGF-β1-Smad-1-7信号抑制上皮间质转化可减缓肺癌进展

Journal of cancer science and clinical therapeutics Pub Date : 2023-01-01 DOI: 10.26502/jcsct.5079192

Ritu Kulshrestha, Pawan Kumar, Ashutosh Kumar Singh, Divya S Nair, M. R., Himanshu Dhanda, Apoorva Pandey, D. R., Mishra Ak, Dinda Ak

引用次数: 0

Percutaneous irreversible Electroporation in Locally Advanced Pancreatic Cancer: A Review of Current Literature 局部晚期胰腺癌的经皮不可逆电穿孔:当前文献综述

Journal of cancer science and clinical therapeutics Pub Date : 2023-01-01 DOI: 10.26502/jcsct.5079206

S. Carriero, C. Lanza, G. Pellegrino, Caterina Sattin

引用次数: 0

Liver Endothelium Microenvironment Promotes HER3-mediated Cell Growth in Pancreatic Ductal Adenocarcinoma. 肝脏内皮微环境促进 HER3 介导的胰腺导管腺癌细胞生长

Journal of cancer science and clinical therapeutics Pub Date : 2022-01-01 Epub Date: 2022-12-15 DOI: 10.26502/jcsct.5079182

Moeez Rathore, Wei Zhang, Michel'le Wright, Mehrdad Zarei, Ali Vaziri-Gohar, Omid Hajihassani, Ata Abbas, Hao Feng, Jonathan Brody, Sanford D Markowitz, Jordan Winter, Rui Wang

{"title":"Liver Endothelium Microenvironment Promotes HER3-mediated Cell Growth in Pancreatic Ductal Adenocarcinoma.","authors":"Moeez Rathore, Wei Zhang, Michel'le Wright, Mehrdad Zarei, Ali Vaziri-Gohar, Omid Hajihassani, Ata Abbas, Hao Feng, Jonathan Brody, Sanford D Markowitz, Jordan Winter, Rui Wang","doi":"10.26502/jcsct.5079182","DOIUrl":"10.26502/jcsct.5079182","url":null,"abstract":"~90% metastatic pancreatic ductal adenocarcinoma (mPDAC) occurs in the liver, and the 5-year survival rate for patients with mPDAC is only at 3%. The liver has a unique endothelial cell (EC)-rich microenvironment, and preclinical studies showed that ECs promote cancer cell survival pathways by secreting soluble factors in a paracrine fashion in other types of cancer. However, the effects of liver ECs on mPDAC have not been elucidated. In this study, we used primary liver ECs and determined that liver EC-secreted factors containing conditioned medium (CM) increased PDAC cell growth, compared to control CM from PDAC cells. Using an unbiased receptor tyrosine kinase array, we identified human epidermal growth factor receptor 3 (HER3, also known as ErbB3) as a key mediator of liver EC-induced growth in PDAC cells with HER3 expression (HER3 +ve). We found that EC-secreted neuregulins activated the HER3-AKT signaling axis, and that depleting neuregulins from EC CM or blocking HER3 with an antibody, seribantumab, attenuated EC-induced functions in HER3 +ve PDAC cells, but not in cells without HER3 expression. Furthermore, we determined that EC CM increased PDAC xenograft growth in vivo, and that seribantumab blocked EC-induced growth in xenografts with HER3 expression. These findings elucidated a paracrine role of liver ECs in promoting PDAC cell growth, and identified the HER3-AKT axis as a key mediator in EC-induced functions in HER3 +ve PDAC cells. As over 70% mPDAC express HER3, this study highlights the potential of using HER3-targeted therapies for treating patients with HER3 +ve mPDAC.","PeriodicalId":73634,"journal":{"name":"Journal of cancer science and clinical therapeutics","volume":"6 4","pages":"431-445"},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9838560/pdf/nihms-1860370.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10595197","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0