Journal of biological methods最新文献

{"title":"Nanotechnology-based approaches for targeted drug delivery for the treatment of respiratory tract infections.","authors":"Vasiliki Epameinondas Georgakopoulou, Petros Papalexis, Nikolaos Trakas","doi":"10.14440/jbm.2024.0065","DOIUrl":"10.14440/jbm.2024.0065","url":null,"abstract":"<p><strong>Background: </strong>Nanotechnology has emerged as a promising field for the diagnosis, monitoring, and treatment of respiratory tract infections (RTIs). By leveraging the unique properties of nanoscale delivery systems, nanotechnology can significantly enhance the selectivity and efficacy of antimicrobials, thereby reducing off-target effects.</p><p><strong>Objective: </strong>This review explores the development and application of targeted nanosystems in combating viral, bacterial, and fungal RTIs. Nanotechnology-based systems, including biological and non-biological nanoparticles, offer innovative solutions for overcoming antimicrobial resistance, improving drug bioavailability, and minimizing systemic side effects. RTIs are a leading cause of morbidity and mortality globally, particularly affecting vulnerable populations such as children, the elderly, and immunocompromised individuals. Traditional drug delivery methods face numerous challenges, such as rapid clearance, poor tissue penetration, and drug degradation. Nanoparticle-based delivery systems address these issues by enhancing tissue penetration, providing sustained drug release, and enabling targeted delivery to infection sites. These systems include liposomal delivery, polymeric nanoparticles, dendrimers, and metal-based nanoparticles, each offering unique advantages in treating RTIs. Nanotechnology also plays a crucial role in vaccine development by offering new strategies to enhance immune responses and improve antigen delivery. Furthermore, the review discusses the clinical translation and regulatory considerations for nanotechnology-based drug delivery, emphasizing the need for rigorous testing and quality control to ensure safety and efficacy.</p><p><strong>Conclusion: </strong>Nanotechnology offers promising advancements in the treatment, and prevention of RTIs by enhancing drug delivery and efficacy. By addressing challenges such as antimicrobial resistance and poor tissue penetration, nanotechnology-based systems have the potential to significantly improve patient outcomes.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"11 4","pages":"e99010032"},"PeriodicalIF":0.0,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744063/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017688","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Full mouth rehabilitation with fixed teeth supported prosthesis following hobo twin stage technique - A Case Report.","authors":"Ahila Singaravel Chidambaranathan, Rajdeep Tah, Muthu Kumar Balasubramanium","doi":"10.14440/jbm.2024.0036","DOIUrl":"10.14440/jbm.2024.0036","url":null,"abstract":"<p><strong>Background: </strong>Worn dentition is a common problem encountered by most people in the last decade. Rehabilitation of a full mouth needs individual attention and proper treatment planning, which is very challenging for partial edentulous cases where bilateral teeth are missing and collapsed vertical dimension leaves inadequate restorative space. Treatment of these cases is complex and needs to apply standard principles while designing and fabricating prostheses.</p><p><strong>Case presentation: </strong>A 41-year-old female presented with a history of difficulty in chewing and poor appearance. Examination revealed that the patient had missing teeth in the maxilla (12, 13, 14, 15, 23, and 24), in the mandible (46 and 47), and in teeth 11, 21, 22, 25, 36, 37, 43, 44, and 45 were root canal treated, of which 21, 22, and 25 did not have any coronal structure and 36 had a discharging sinus. The periodontal condition of the remaining teeth was good and the vertical dimension was reduced. This patient had multiple missing and badly damaged anterior teeth. Hence, Hobo and Takayama twin stage method was used to re-establish the anterior guidance for full mouth rehabilitation.</p><p><strong>Conclusion: </strong>Worn dentition requires a very careful and calculative approach due to its complex nature. Hobo twin stage technique is based on case studies from which the mathematical values for anterior guidance and posterior disocclusion are determined and thus minimizes the clinical procedure duration and produces good masticatory function.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"11 4","pages":"e99010027"},"PeriodicalIF":0.0,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017679","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of health-promoting behaviors and menopausal symptoms of urban women of Hyderabad: A randomized controlled trial.","authors":"Sudha Bala, Satya Lakshmi Komarraju, Sathyanath Dasarathan, Shrikanth Muralidharan","doi":"10.14440/jbm.2024.0041","DOIUrl":"10.14440/jbm.2024.0041","url":null,"abstract":"<p><strong>Background: </strong>Menopause, characterized by cessation of menstruation, is accompanied by various physiological and psychological changes. This randomized controlled trial (RCT) investigated the efficacy of a multi-domain lifestyle modification program in alleviating menopausal symptoms and promoting health behaviors among postmenopausal women residing in urban slums of Hyderabad, India.</p><p><strong>Methods: </strong>Eighty-two women aged 45-60 years experiencing menopausal symptoms were recruited. Participants were randomly assigned to either a 45-day lifestyle intervention group or a control group receiving standard care. The intervention program addressed six domains: health responsibility, physical activity, nutrition, spiritual growth, interpersonal relations, and stress management. The menopause rating scale (MRS) and a modified health-promoting lifestyle II (HPBII) questionnaire assessed menopausal symptoms and health behaviors at baseline and during follow-up.</p><p><strong>Results: </strong>The intervention group showed significant improvements (<i>P</i> < 0.05) in terms of overall HPBII scores compared to baseline, with the most notable changes observed in health responsibility, nutrition, and stress management domains. In addition, MRS scores indicating menopausal symptoms were significantly lowered (<i>P</i> < 0.05) in the intervention group. No significant changes were found in the control group in either health behaviors or menopausal symptoms. After the intervention, the experimental group showed a significant improvement in HPBII scores compared to the control group, particularly in health responsibility, nutrition, interpersonal relationships, and stress management sub-domains.</p><p><strong>Conclusion: </strong>This RCT suggested that a comprehensive lifestyle modification program could effectively improve health behaviors and alleviate menopausal symptoms in postmenopausal women residing in under-resourced settings. Further research is warranted to explore the program's long-term sustainability and generalizability.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"11 3","pages":"e99010028"},"PeriodicalIF":0.0,"publicationDate":"2024-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557300/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Lower urinary tract dysfunction following stroke: From molecular mechanisms to clinical anatomy.","authors":"Eleni Agapiou, Efstratios-Stylianos Pyrgelis, Ioannis N Mavridis, Maria Meliou, Welege Samantha Buddhika Wimalachandra","doi":"10.14440/jbm.2024.0022","DOIUrl":"10.14440/jbm.2024.0022","url":null,"abstract":"<p><p>Bladder dysfunction is a common clinical problem in stroke patients and a strong prognostic factor of disability and exerts an enormous impact on health and economy. The aim of this narrative review was tο examine the pathophysiological mechanisms of lower urinary tract symptoms after stroke, as well as the relevant clinical anatomy. Normal micturition is achieved through complex coordination between brain regions, spinal cord, and peripheral nerves, and anatomic brain connectivity is crucial to lower urinary tract physiology. The most important neurotransmitters involved in bladder control include γ-aminobutyric acid, opioids, glutamate, dopamine, norepinephrine, acetylcholine, and nitric oxide. The precise correspondence between brain damage and relevant urinary symptoms is not well understood. Urodynamic changes after stroke include detrusor overactivity, dyssynergia, and uninhibited sphincter relaxation. Several brain regions could be implicated in post-stroke urinary dysfunction. Brainstem lesions can cause various urinary symptoms. A lesion superiorly to the pontine micturition center (PMC) results in an uninhibited bladder, whereas a lesion between the sacral spinal cord and PMC leads to either a spastic bladder or sphincter-detrusor dyssynergia. Supra-pontine lesions usually cause bladder storage dysfunction. Frontoparietal lesions have been associated with urinary incontinence and insular lesions with urinary retention. Understanding the mechanisms underlying the dysfunction of the lower urinary tract following stroke can aid in the development of new therapeutic strategies for these patients.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"11 4","pages":"e99010024"},"PeriodicalIF":0.0,"publicationDate":"2024-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of positioning accuracy in head-and-neck cancer treatment: A cone beam computed tomography assessment of three immobilization devices with volumetric modulated arc therapy.","authors":"Noor Mail, Khalid M Alshamrani, Rab Nawaz Lodhi, Eman Khawandanh, Amani Saleem, Batoor Khan, Majed Alghamdi, Mohammed Nadershah, Majid S Althaqafy, Ahmed Subahi, Suliman M Alghamdi","doi":"10.14440/jbm.2024.0047","DOIUrl":"10.14440/jbm.2024.0047","url":null,"abstract":"<p><p>In this study, we assessed the precision and repeatability of the daily patient positioning for three distinct immobilization devices used for head-and-neck patients undergoing RapidArc radiation therapy using cone beam computed tomography (CBCT). An analysis was conducted on the accuracy of patient setup for three distinct immobilization devices, resulting in 1204 CBCT images for 189 patients in total. Using a typical posifix supine headrest and five fixation point podcast-plus-thermoplastic masks, the first group of 39 patients (125 CBCTs) was immobilized. The identical method was used to immobilize the second group of 19 patients (158 CBCTs) in the same posture (supine), and AccuFormTM custom headrests were employed as an added measure. Over 65% of the patients in the third group had a double shell positioning system (DSPS) covering their entire head and neck. Patient-alignment-accuracy or couch shifts in the vertical, longitudinal, and lateral directions from CT-CBCT fusions were recorded from ARIA. Our results showed that in 90% of the anteriorposterior (AP), 90% of the superior-inferior (SI), and 92.7% of the right-left (RL) population in the first group, patient-alignment-accuracy or couch shifts were within 2 mm. For 99.4% (AP), 100% (SI), and 98.7% (RL) of the second group's total population, patient-alignment-accuracy was within 2 mm. In the third group, it was within 2 mm for 92.1% (AP), ~89% (SI), and 93.3% (RL) of the total population. In conclusion, a significant improvement was seen with the application of a mouth bite and a tailored backrest cushion to the five fixation point posicast mask. In addition, significant improvement in the alignment of the lower neck area was observed with the use of DSPS. Virtually 100% of the head-and-neck patients were aligned within an accuracy of 3 mm, which is the PTV margin in our department.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"11 3","pages":"e99010025"},"PeriodicalIF":0.0,"publicationDate":"2024-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557299/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The neuronal density in the rostral pole of substantia nigra pars compacta in Wistar Albino rats from Rijswijk rats: A link to spike-wave seizures.","authors":"Lidia M Birioukova, Darya A Tsvetaeva, Inna S Midzyanovskaya, Vladimir V Raevsky, Evgenia Sitnikova","doi":"10.14440/jbm.2024.0027","DOIUrl":"10.14440/jbm.2024.0027","url":null,"abstract":"<p><p>This study aimed to investigate the role of the nigrostriatal dopaminergic system in the modulation of absence epilepsy. Immunochemical analysis of the rostral pole of the substantia nigra pars compacta (SNpc) was conducted on 13 adult male Wistar Albino rats from Rijswijk rats. The rostral pole of the SNpc included the dorsal and lateral parts. The neuronal density in the dorsal part was higher than in the lateral part. The ratio of dopaminergic to non-dopaminergic neurons in the lateral part of the SNpc was 1:1, while in the dorsal part, it was around 1.9:1. All rats exhibited spontaneous spike-wave discharges (SWDs) on their electrocorticograms. SWDs are known to be a hallmark of absence seizures in both human patients and rat models. In this study, we found that the number and duration of SWDs were negatively correlated with dopaminergic and non-dopaminergic neurons only in the lateral part of the SNpc. However, in the dorsal part of the SNpc, no correlations were found between neuronal density and the severity of absence epilepsy. Our findings suggest that the lateral SNpc may be involved in modulating the severity of absence epilepsy in genetically prone subjects. This contributes to a better understanding of the role of the nigrostriatal dopaminergic system in the absence of epilepsy.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"11 3","pages":"e99010022"},"PeriodicalIF":0.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557302/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genetic insights into endurance athlete status: A meta-analysis of <i>ACVR1B, AGT, FTO, IL-6, and NRF2</i> gene polymorphisms.","authors":"Gökhan İpekoğlu, Erdal Ari, Alpay Aydoğdu, Furkan Korkmaz, Hülya Çelik, Fatih Öztürk, Şeyma Tuba Acar","doi":"10.14440/jbm.2024.0013","DOIUrl":"10.14440/jbm.2024.0013","url":null,"abstract":"<p><p>The aim of this meta-analysis was to analyze allele and genotype pattern frequencies of five different gene polymorphisms associated with endurance athlete status. Endurance sports require prolonged physical performance, and it is well known that physiological and genetic characteristics play a prominent role in this performance. In recent years, research on the impact of genetic factors on endurance athlete status has been on the rise, suggesting that various gene polymorphisms may be associated with physical performance. Specifically, the gene polymorphisms, activin receptor type-1B (<i>A9CVR1B</i>) rs2854464, angiotensinogen (<i>AGT</i>) rs699, fat mass and obesity-associated (<i>FTO</i>) rs9939609, interleukin-6 (<i>IL-6</i>) rs1800795, and nuclear factor erythroid 2 (<i>NRF2</i>) rs12594956, are thought to be related to endurance athlete status. Each of these genes plays a part in different biological processes, such as muscle development, energy metabolism, inflammation, and antioxidant defense mechanisms. For example, the <i>NRF2</i> gene is a critical player in the regulation of cellular stress responses and may contribute to adaptive responses that enhance performance in endurance athletes. This meta-analysis included a total of 20 articles published between 2009 and 2023. The specific gene polymorphisms explored in this study, i.e., <i>ACVR1B</i> rs2854464, <i>AGT</i> rs699, <i>FTO</i> rs9939609, <i>IL-6</i> rs1800795, and <i>NRF2</i> rs12594956, were selected due to their reported associations with physical performance and endurance. A comprehensive search was conducted in the Web of Science and PubMed databases using specific keywords, preliminarily identifying 329 articles. Upon analysis of the abstracts, and full texts, 20 articles were deemed eligible for inclusion in this meta-analysis. Articles lacking control and endurance athlete groups or clear allele/genotype data were excluded. The findings indicated no significant differences in allele and genotype frequencies for <i>ACVR1B</i>, <i>AGT</i>, <i>FTO</i>, and <i>IL-6</i> gene polymorphisms between endurance athletes and control groups. However, the <i>NRF2</i> rs12594956 gene polymorphism showed a significantly higher frequency of the major allele (A) and the AA genotype in endurance athletes than in controls. In conclusion, the <i>NRF2</i> rs12594956 polymorphism may be a genetic variant of interest in determining the status of endurance athletes. These findings highlight the potential clinical implications for genetic screening and personalized training programs in sport genetics. More extensive studies with larger cohorts are needed to further confirm these associations.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"11 3","pages":"e99010021"},"PeriodicalIF":0.0,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557301/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634090","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Improving CT scan for lung cancer diagnosis with an integromic signature.","authors":"Jipei Liao, Pushpawallie Dhilipkannah, Feng Jiang","doi":"10.14440/jbm.2024.0028","DOIUrl":"10.14440/jbm.2024.0028","url":null,"abstract":"<p><p>Lung cancer is the leading cause of cancer-related mortality globally, making early detection crucial for reducing death rates. Low-dose computed tomography (LDCT) screening helps detect lung cancer early but often identifies indeterminate pulmonary nodules (PNs), leading to potential overtreatment. This study aimed to develop a diagnostic test that accurately differentiates malignant from benign PNs detected on LDCT scans by analyzing non-coding RNAs, DNA methylation, and bacterial DNA in patient samples. Using droplet digital polymerase chain reaction, we analyzed samples from a training set of 150 patients with malignant PNs and 250 smokers with benign PNs. Individual biomarkers in plasma and sputum showed moderate effectiveness, with sensitivities ranging from 62% to 77% and specificities from 54% to 87%. We developed an integromic signature by combining two plasma biomarkers and one sputum biomarker, along with additional clinical data, which demonstrated a sensitivity of 90% and specificity of 95%. The signature's diagnostic performance was further validated in a cohort consisting of 30 patients with malignant PNs and 50 smokers with benign PNs. The integromic signature showed high sensitivity and specificity in distinguishing malignant from benign PNs identified through LDCT. This tool has the potential to significantly lower both mortality and health-care costs associated with the overtreatment of benign nodules, offering a promising approach to improving lung cancer screening protocols.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"11 3","pages":"e99010023"},"PeriodicalIF":0.0,"publicationDate":"2024-09-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634100","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CH1-specific affinity resins possess the potential of separating heterodimer from homodimers in asymmetric bispecific antibody purification.","authors":"Wanyuan Dong, Yifeng Li","doi":"10.14440/jbm.2024.0026","DOIUrl":"10.14440/jbm.2024.0026","url":null,"abstract":"<p><p>CaptureSelect CH1-XL and Praesto 70 CH1 are two affinity media that specifically bind to the CH1 domain of an antibody. In the current work, we first demonstrated that these two CH1-specific affinity media bound to different monoclonal antibodies (mAbs) with varied strengths under identical conditions. We previously had observed the same on a Protein L-conjugated resin and showed that such a property could facilitate homodimer removal in asymmetric bispecific antibody (bsAb) purification. Next, using Praesto 70 CH1, we showed that a small difference in binding between two mAbs could be significantly exaggerated by adding sodium chloride to the mobile phase, further demonstrating this resin can potentially play a role in bsAb purification. Finally, with a concrete bsAb case study, we showed that, like Protein L, Praesto 70 CH1 could separate the target heterodimer from the homodimer by-product. Homodimers are common product-related impurities associated with the recombinant production of asymmetric bsAbs, which can be difficult to remove. Their removal, even a partial one, at the capture stage is a big advantage as it can alleviate the purification burden on subsequent polishing steps and render the overall process more robust. Therefore, Praesto 70 CH1's unique property is highly desirable, and this affinity resin can be a better alternative than Protein A for product capture in asymmetric bsAb purification.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"11 3","pages":"e99010020"},"PeriodicalIF":0.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557294/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The development and application of cleavage under targets and tagmentation (CUT&Tag) technology.","authors":"Chaoyang Xiong, Jiyin Wang, Xinglin Li, Guohong Li, Xi Wang","doi":"10.14440/jbm.2024.0017","DOIUrl":"10.14440/jbm.2024.0017","url":null,"abstract":"<p><p>The regulation of chromatin structure and gene transcription in eukaryotic cells is an involved process mediated by histone modifications, chromatin-binding proteins, and transcription factors. Studying the distribution of histone modifications and transcription factors at the whole-genome level is crucial for understanding the mechanisms of gene transcription. Cleavage under targets and tagmentation (CUT&Tag) is a novel chromatin analysis method that has rapidly gained popularity in the field of epigenetics since its introduction. It has been widely used for the detection of chromatin modifications and transcription factors in different species. Furthermore, CUT&Tag has also been adapted to simultaneously detect multiple epigenetic modifications. Its integration with single-cell transcriptome, and spatial transcriptome analysis allows for a comprehensive examination of cell fate and functions. In this review, we aimed to provide an overview of the recent developments and applications of CUT&Tag and its derivatives, highlighting their significance in advancing our understanding of epigenetic regulation.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"11 3","pages":"e99010019"},"PeriodicalIF":0.0,"publicationDate":"2024-09-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11557298/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}