Journal of biological methods最新文献

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Integration of an automated cell culture analyzer with a closed-system hollow-fiber bioreactor for online metabolite detection and cell monitoring. 自动化细胞培养分析仪与封闭系统中空纤维生物反应器的集成,用于在线代谢物检测和细胞监测。
Journal of biological methods Pub Date : 2025-03-04 eCollection Date: 2025-01-01 DOI: 10.14440/jbm.2025.0046
Nathan Schwab, Nathan Frank, Tonya Acker, Kelly Richardson, Mindy Miller
{"title":"Integration of an automated cell culture analyzer with a closed-system hollow-fiber bioreactor for online metabolite detection and cell monitoring.","authors":"Nathan Schwab, Nathan Frank, Tonya Acker, Kelly Richardson, Mindy Miller","doi":"10.14440/jbm.2025.0046","DOIUrl":"https://doi.org/10.14440/jbm.2025.0046","url":null,"abstract":"<p><strong>Background: </strong>On-line sensing technologies for expanding cell cultures are becoming essential tools for understanding metabolic activity during critical stages of expansion. These tools generate data that indicate when user interventions are required, such as harvesting cells or cell products, inducing cell differentiation, or altering growth medium inputs. The platform must reliably measure the biochemical and physiochemical properties of interest in a dependable, aseptic, and non-invasive manner to benefit users.</p><p><strong>Objective: </strong>In this proof-of-concept study, we used the Nova Biomedical BioProfile FLEX2 as a platform for metabolic and cellular measurements due to its ability to detect a range of metabolically relevant compounds, measure cell counts and viability, and acquire samples automatically.</p><p><strong>Methods: </strong>Here, we demonstrated the straightforward integration of the analyzer with the Quantum Flex™ Cell Expansion System (Quantum Flex) using an available sampling port. While this approach can accommodate both suspension and adherent cell types, this study focused on suspension cells only. In addition, we developed a simple method to integrate the sampling adapter into a Quantum cell expansion set, allowing sample collection at precise intervals.</p><p><strong>Results: </strong>In both cases, the samples were acquired automatically using the analyzer's timing function, facilitating an automated expansion process with increased data collection frequency - previously impractical with manual sampling.</p><p><strong>Conclusion: </strong>This model provides Quantum Flex users with an option for online sensing to monitor cell expansion at scheduled intervals without requiring additional user input.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"12 1","pages":"e99010043"},"PeriodicalIF":0.0,"publicationDate":"2025-03-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973053/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143813127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Screening of organic substrates for the development of effective biofungicides to manage cashew fusarium wilt disease. 筛选有机基质,开发有效的生物杀菌剂防治腰果枯萎病。
Journal of biological methods Pub Date : 2025-02-21 eCollection Date: 2025-01-01 DOI: 10.14440/jbm.2025.0089
Stanslaus A Lilai, Juma Hussein, Fortunus A Kapinga, Wilson A Nene, Stela G Temu, Donatha D Tibuhwa
{"title":"Screening of organic substrates for the development of effective biofungicides to manage cashew fusarium wilt disease.","authors":"Stanslaus A Lilai, Juma Hussein, Fortunus A Kapinga, Wilson A Nene, Stela G Temu, Donatha D Tibuhwa","doi":"10.14440/jbm.2025.0089","DOIUrl":"https://doi.org/10.14440/jbm.2025.0089","url":null,"abstract":"<p><strong>Background: </strong>The biotechnology industry commonly utilizes synthetic media to grow biological control agents (BCAs); however, these media are often considered impractical, particularly in developing countries.</p><p><strong>Objective: </strong>This study aimed to identify the most suitable locally available organic substrates for the cultivation of BCAs used against cashew fusarium wilt disease.</p><p><strong>Materials and methods: </strong>Experiments were conducted in 2022 and 2023 on five substrates, namely rice husk (RH), rice bran (RB), hulled millet, parboiled rice, and their combinations, as growth media for combined <i>Bacillus</i> strains and <i>Trichoderma asperellum</i>. The carbohydrate and protein content of the substrates were quantified colorimetrically.</p><p><strong>Results: </strong>Protein and carbohydrate contents ranged from 13.4 - 19.8% to 76.3 - 82.7%, respectively. The BCAs exhibited high colonization rates on all tested substrates, with combined substrates supporting the highest colonization, followed by RH and RB. Endospore formation and conidiation levels varied significantly over time across substrates and incubation temperatures (<i>p</i> ≤ 0.05). Population densities decreased over time under refrigerated, room temperature, and incubator conditions for most substrates in both seasons, except for combined substrates, RH, and RB. Final population counts were as follows: 2.1 × 10<sup>7</sup> ± 4.9 × 10<sup>5</sup> and 1.0 × 10<sup>7</sup> ± 0.3 × 10<sup>5</sup> colony-forming units (CFUs)/mL (combined substrates), 2.3 × 10<sup>7</sup> ± 5.4 × 10<sup>5</sup> and 5.7 × 10<sup>7</sup> ± 1.1 × 10<sup>6</sup> CFUs/mL (RH), 3.6 × 10<sup>5</sup> ± 2.1 × 10<sup>4</sup> and 3.3 × 10<sup>5</sup> ± 1.6 × 10<sup>4</sup> CFUs/mL (RB), while under refrigerated conditions, population densities remained relatively unchanged throughout the study period.</p><p><strong>Conclusion: </strong>Based on the findings, it is recommended to use a mixture of substrates, RH alone, or RB alone as appropriate media for the large-scale production of the studied biofungicides.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"12 1","pages":"e99010053"},"PeriodicalIF":0.0,"publicationDate":"2025-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812558","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Platelet-rich plasma as a promising bioscaffold for enhancing peripheral nerve regeneration: An experimental study in a rat sciatic nerve model. 富血小板血浆作为促进周围神经再生的生物支架:大鼠坐骨神经模型的实验研究。
Journal of biological methods Pub Date : 2025-02-19 eCollection Date: 2025-01-01 DOI: 10.14440/jbm.2025.0083
Sofija Pejkova, Velimir Stojkovski, Gordana Georgieva, Boris Aleksovski, Sofija Tusheva, Blagoja Srbov, Elena Rafailovska, Stefania Azmanova Mladenovska, Katerina Jovanovska, Bisera Nikolovska, Boro Ilievski, Boro Dzonov, Trpe Ristoski, Anita Petrushevska, Icko Gjorgoski
{"title":"Platelet-rich plasma as a promising bioscaffold for enhancing peripheral nerve regeneration: An experimental study in a rat sciatic nerve model.","authors":"Sofija Pejkova, Velimir Stojkovski, Gordana Georgieva, Boris Aleksovski, Sofija Tusheva, Blagoja Srbov, Elena Rafailovska, Stefania Azmanova Mladenovska, Katerina Jovanovska, Bisera Nikolovska, Boro Ilievski, Boro Dzonov, Trpe Ristoski, Anita Petrushevska, Icko Gjorgoski","doi":"10.14440/jbm.2025.0083","DOIUrl":"https://doi.org/10.14440/jbm.2025.0083","url":null,"abstract":"<p><strong>Background: </strong>Despite advancements in surgical treatments, impairments persist after peripheral nerve injuries, prompting a shift in research toward the microenvironment of injured axons. Platelet-rich plasma (PRP), rich in growth factors and derived from autologous blood, emerges as a potential candidate to accelerate nerve healing.</p><p><strong>Objective: </strong>This study investigated the role of PRP in enhancing peripheral nerve regeneration using a rat sciatic nerve model (<i>n</i> = 8) in female Wistar rats.</p><p><strong>Methods: </strong>A transected sciatic nerve model was created, with both hindlimbs repaired through end-to-end neurorrhaphy. PRP, prepared from the blood of a healthy Wistar rat, was applied to one hindlimb. Functional recovery was assessed using sciatic indices. At the 20-week time point, histological evaluations were performed to compare PRP-treated hindlimbs with control ones. Statistical analysis was conducted to compare the results between the two groups using three different calculations for specific parameters.</p><p><strong>Results: </strong>Walking track-based sciatic functional index (SFI) showed an improvement of 66.0%, 47.8%, and 71.6% (<i>p</i> < 0.05). Video analysis-based SFI revealed a 36.7% and 27.3% improvement (<i>p</i> < 0.05). Static sciatic index calculations indicated an improvement of 19.4% for vertical standing and 26.7% for standing on all four limbs (<i>p</i> < 0.001). Histopathological analysis showed a reduction in inflammation, a decrease in fibrosis, and the absence of macrophages in the sciatic nerves of the experimental group. Muscle specimens from the PRP-treated group exhibited fewer macrophages and significantly less fibrosis (<i>p</i> < 0.05). Overall, PRP treatment significantly improved all functional indices.</p><p><strong>Conclusion: </strong>This study demonstrated PRP's utility in promoting peripheral nerve regeneration, highlighting its potential for both fundamental research and clinical applications.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"12 1","pages":"e99010051"},"PeriodicalIF":0.0,"publicationDate":"2025-02-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143813149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
3D visualization of human colon tissue using a modified CUBIC-based tissue-clearing technique. 使用改进的基于立方体的组织清除技术的人类结肠组织的三维可视化。
Journal of biological methods Pub Date : 2025-02-04 eCollection Date: 2025-01-01 DOI: 10.14440/jbm.2025.0101
Pavel Pavlov, Andreas Kontny, Neele Wagner, Nikola Kolev, Alexander Zlatarov, Turgay Kalinov, Anton B Tonchev
{"title":"3D visualization of human colon tissue using a modified CUBIC-based tissue-clearing technique.","authors":"Pavel Pavlov, Andreas Kontny, Neele Wagner, Nikola Kolev, Alexander Zlatarov, Turgay Kalinov, Anton B Tonchev","doi":"10.14440/jbm.2025.0101","DOIUrl":"https://doi.org/10.14440/jbm.2025.0101","url":null,"abstract":"<p><strong>Background: </strong>Colorectal cancer represents one of the most common neoplastic diseases worldwide, making it a frequent focus in routine pathological analyses. Visualizing complex three-dimensional (3D) structures, such as nerves within tumors, requires thick tissue sections, which necessitates the use of optical tissue-clearing methods to achieve transparency. However, following tissue clearing, samples typically require advanced imaging techniques such as light-sheet and two-photon confocal microscopy, which are usually unavailable in standard histological laboratories.</p><p><strong>Objective: </strong>We aimed to demonstrate how a well-established tissue-clearing approach can be adapted for use in a routine histological laboratory, enabling a robust 3D visualization of nerve fibers in samples of both normal human colon and colon cancer tissues.</p><p><strong>Methods: </strong>We modified the \"clear unobstructed brain/body imaging cocktails\" method, originally developed for whole-brain imaging in mice, and applied it to human colon tissue samples measuring approximately 10 mm<sup>3</sup>, a standard size typically processed in pathological laboratories.</p><p><strong>Results: </strong>Our protocol, which integrates a tissue-clearing technique, enabled reliable immunofluorescent visualization of colonic nerve fibers labeled with anti-β<sub>3</sub>-tubulin antibodies. The labeled nerve fibers could be observed using a standard epifluorescence microscope, and high-quality 3D reconstructions were generated through a simple image analysis approach using the open-source software ilastik, which eliminates the need for confocal microscopy.</p><p><strong>Conclusion: </strong>The proposed steps provide a valuable method for researchers to visualize complex 3D structures, such as neural cells and processes, in both normal and tumor-transformed tissue settings.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"12 1","pages":"e99010052"},"PeriodicalIF":0.0,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973050/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143813134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Phylodynamics of hepatitis B virus genotype B in East Asia: A population genomics analysis. 东亚乙型肝炎病毒基因型的系统动力学:种群基因组学分析。
Journal of biological methods Pub Date : 2025-01-17 eCollection Date: 2025-01-01 DOI: 10.14440/jbm.2025.0084
Serena Y C Lin, Patrick C Y Woo
{"title":"Phylodynamics of hepatitis B virus genotype B in East Asia: A population genomics analysis.","authors":"Serena Y C Lin, Patrick C Y Woo","doi":"10.14440/jbm.2025.0084","DOIUrl":"https://doi.org/10.14440/jbm.2025.0084","url":null,"abstract":"<p><strong>Background: </strong>Hepatitis B virus (HBV) genotype B (HBV/B) is the predominant strain in Taiwan and several East Asian countries.</p><p><strong>Objective: </strong>The aim of this study is to use comprehensive phylogenetic analysis tools to monitor the long-term molecular evolution dynamic of HBV genotype B population in East Asia.</p><p><strong>Methods: </strong>In this study, full genome sequences of HBV with temporal information were extracted from GenBank and analyzed using the Bayesian Markov chain Monte Carlo method to identify best-fitting coalescent models.</p><p><strong>Results: </strong>Bayesian Skygrid analysis revealed a viral effective population (phylodynamic) bottleneck for HBV/B in 2003, a pattern similar to the previously described HBV genotype C (HBV/C). Despite these similarities, the viral dynamics for HBV/B and HBV/C diverged after 2005. HBV/C exhibited a marked decrease in genetic diversity across East Asia, whereas HBV/B maintained stable genetic diversity after 2005. Phylogeographic analysis using Neighbor-Joining and Bayesian maximum clade credibility trees indicated that Taiwan was likely the geographic origin of the most recent common ancestor of HBV/B in East Asia. An early clade spread to Japan and subsequently to the West Coast of the United States of America. Another clade dispersed to China, spread widely across the region, and was reintroduced to Taiwan multiple times. In contrast, HBV/C likely originated in China and spread to Japan, Korea, and Taiwan over several decades.</p><p><strong>Conclusion: </strong>This study highlights the similarities and differences between the viral dynamics and geographical evolutionary pathways between HBV/B and HBV/C.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"12 1","pages":"e99010048"},"PeriodicalIF":0.0,"publicationDate":"2025-01-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973052/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143813143","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Lipidomics and temporal-spatial distribution of organelle lipid. 脂质组学与细胞器脂质的时空分布。
Journal of biological methods Pub Date : 2025-01-16 eCollection Date: 2025-01-01 DOI: 10.14440/jbm.2025.0094
Wenjuan Qian, Huiru Tang, Hongyan Yao
{"title":"Lipidomics and temporal-spatial distribution of organelle lipid.","authors":"Wenjuan Qian, Huiru Tang, Hongyan Yao","doi":"10.14440/jbm.2025.0094","DOIUrl":"https://doi.org/10.14440/jbm.2025.0094","url":null,"abstract":"<p><strong>Background: </strong>Lipids are crucial signaling molecules or cellular membrane components orchestrating biological processes. To gain insights into lipid functions and the communication between organelles, it is essential to understand the subcellular localization of individual lipids. Advancements in lipid quantification techniques, improvements in chemical and spatial resolution for detecting various lipid species, and enhancements in organelle isolation speed have allowed for profiling of the organelle lipidome, capturing its temporal-spatial distribution.</p><p><strong>Objective: </strong>This review examined approaches used to develop organelle lipidome and aimed to gain insights into cellular lipid homeostasis from an organelle perspective. In addition, this review discussed the advancements in lipid-mediated inter-organelle communication within complex physiological and pathological processes.</p><p><strong>Conclusion: </strong>With the advancement of lipidomic technologies, more detailed explorations of organelle structures and the specific lipid-mediating functions they perform are feasible.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"12 1","pages":"e99010049"},"PeriodicalIF":0.0,"publicationDate":"2025-01-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973048/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143813130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Reappraisal of the fundamental mechanisms of the sHA14-1 molecule as a Bcl-2/Bcl-XL ligand in the context of anticancer therapy: A cell biological study. 重新评估sHA14-1分子作为抗癌治疗背景下Bcl-2/Bcl-XL配体的基本机制:一项细胞生物学研究
Journal of biological methods Pub Date : 2024-12-30 eCollection Date: 2024-01-01 DOI: 10.14440/jbm.2024.0055
Aoula Moustapha, Pauline Andreu, François Gonzalvez, Delphine Fradin, Jean-Pierre Tissier, Phillippe Diolez, Patrice Xavier Petit
{"title":"Reappraisal of the fundamental mechanisms of the sHA14-1 molecule as a Bcl-2/Bcl-XL ligand in the context of anticancer therapy: A cell biological study.","authors":"Aoula Moustapha, Pauline Andreu, François Gonzalvez, Delphine Fradin, Jean-Pierre Tissier, Phillippe Diolez, Patrice Xavier Petit","doi":"10.14440/jbm.2024.0055","DOIUrl":"10.14440/jbm.2024.0055","url":null,"abstract":"<p><strong>Background: </strong>HA14-1 is a small-molecule, stable B-cell lymphoma 2 (Bcl-2) antagonist that promotes apoptosis in malignant cells through an incompletely-defined mechanism of action. Bcl-2 and related anti-apoptotic proteins, such as B-cell lymphoma-extra-large [Bcl-XL]), are predominantly localized to the outer mitochondrial membrane, where they regulate cell death pathways. However, the notably short half-life of HA14-1 <i>in vitro</i> limits its potential therapeutic application. To address this limitation, a more stable analog, ethyl-2-amino-6-phenyl-4-(2-ethoxy-2-oxoethyl)-4H-chromene-3-carboxylate (sHA14-1), was developed.</p><p><strong>Objective: </strong>This study investigated the relationship between sHA14-1 and Bcl-2/Bcl-XL. The sHA14-1 molecule acts as a hormetic substance. Therefore, it is crucial to determine whether the hormetic zone corresponds to a putative therapeutic window, that is, the optimal concentration at which sHA14-1 selectively kills cancer cells overexpressing Bcl-2 or Bcl-XL while causing minimal damage to normal cells.</p><p><strong>Methods: </strong>Using classical cell biology and flow cytometry, we examined the main signaling pathways involving Bcl-2 or Bcl-XL, and their modification in the presence of sHA14-1.</p><p><strong>Results: </strong>We showed that sHA14-1 exerted a dual effect on mitochondria: (i) it sensitized cells to increased permeability, and (ii) it inhibited adenosine diphosphate-stimulated respiration and uncoupled respiration. At relatively low concentrations, sHA14-1 induced mitochondrial swelling, reminiscent of \"pore opening\" but with distinct characteristics. Over 30 μM, sHA14-1 caused mitochondrial transition depolarization independent of permeability transition and cell death that resembled secondary necrosis (<i>i.e</i>., occurring after maximal mitochondrial permeability) rather than apoptosis. The balance between apoptotic and necrotic cell death induced by sHA14-1 was also evaluated.</p><p><strong>Conclusion: </strong>Our results suggested that sHA14-1 plays a multifunctional role, involving both mitochondria and the endoplasmic reticulum. Its actions are more complex than its originally intended role in targeting anti-apoptotic Bcl-2 family members, which may complicate its potential application as an anticancer therapy.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"11 4","pages":"e99010040"},"PeriodicalIF":0.0,"publicationDate":"2024-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11744068/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Role of hematological biomarkers in predicting oncological outcomes of definitive chemoradiation in locally advanced vulvar carcinoma. 血液学生物标志物在预测局部晚期外阴癌最终放化疗预后中的作用。
Journal of biological methods Pub Date : 2024-12-18 eCollection Date: 2025-01-01 DOI: 10.14440/jbm.2025.0104
Maysa Al Hussaini, Ramiz Abuhijlih, Issa Mohamad, Abdallah Al-Ani, Fawzi Abuhijla
{"title":"Role of hematological biomarkers in predicting oncological outcomes of definitive chemoradiation in locally advanced vulvar carcinoma.","authors":"Maysa Al Hussaini, Ramiz Abuhijlih, Issa Mohamad, Abdallah Al-Ani, Fawzi Abuhijla","doi":"10.14440/jbm.2025.0104","DOIUrl":"https://doi.org/10.14440/jbm.2025.0104","url":null,"abstract":"<p><strong>Background: </strong>The systemic inflammatory response triggered by the carcinogenic process induces significant changes in a wide range of hematological biomarkers, impacting their levels, functions, and overall roles in the body's physiological and pathological processes.</p><p><strong>Objective: </strong>To evaluate the value of pre-treatment hematological parameters in the prediction of clinical and radiological responses of locally advanced vulvar cancer to definitive chemoradiation.</p><p><strong>Methods: </strong>We retrospectively reviewed the medical records of patients treated at the King Hussein Cancer Center receiving definitive chemoradiation for pathologically confirmed locally advanced vulvar carcinoma. Response of the primary disease to treatment was classified as complete response (CR) if there was no clinically- or radiologically-confirmed residual disease at 12 weeks after completion of chemoradiation. Univariate analyses on complete response, progression-free survival (PFS), and overall survival (OS) were performed using clinical factors and pre-treatment hematological parameters.</p><p><strong>Results: </strong>A total of 30 patients were included, with a median age of 57.5 years and a median follow-up of 21 months. Of these, 24 patients (80%) achieved CR. Disease progression occurred in 11 patients (36.7%) during the follow-up period, and 9 (30%) died. Kaplan-Meier analysis demonstrated that only the neutrophil-to-lymphocyte ratio (NLR) (<i>p</i> = 0.007) and basophil-to-lymphocyte ratio (BLR) (<i>p</i> = 0.05) were predictive of OS. Conversely, PFS was significantly associated with white blood cell count (<i>p</i> = 0.042) and BLR (<i>p</i> = 0.004). Receiver operating characteristic (ROC) analysis indicated that NLR and BLR had significant predictive power for survival at the following cutoffs: 3.4 and 0.035, respectively. When categorized by ROC values, BLR was significantly associated with response to treatment (<i>p</i> = 0.026). Moreover, both NLR and BLR were significantly associated with OS and PFS.</p><p><strong>Conclusion: </strong>Pre-treatment NLR and BLR may be useful predictive markers for clinical and radiological response, as well as for oncological outcomes in locally advanced vulvar cancer treated with definitive chemoradiation.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"12 1","pages":"e99010044"},"PeriodicalIF":0.0,"publicationDate":"2024-12-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973051/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143813165","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
A two-column process for bispecific antibody purification based on MabSelect VL resin's strong byproduct removal capability. 基于MabSelect VL树脂强大的副产物去除能力的双柱工艺纯化双特异性抗体。
Journal of biological methods Pub Date : 2024-12-12 eCollection Date: 2025-01-01 DOI: 10.14440/jbm.2025.0106
Wanyuan Dong, Penglong Zhang, Di Wu, Yan Wan, Yifeng Li
{"title":"A two-column process for bispecific antibody purification based on MabSelect VL resin's strong byproduct removal capability.","authors":"Wanyuan Dong, Penglong Zhang, Di Wu, Yan Wan, Yifeng Li","doi":"10.14440/jbm.2025.0106","DOIUrl":"https://doi.org/10.14440/jbm.2025.0106","url":null,"abstract":"<p><strong>Background: </strong>Protein L-conjugated resins are affinity media that bind to the variable region of the kappa light chain (LC) and have been used for initial product capture in the downstream processing of full-length antibodies and antibody fragments. Previous studies, including ours, have demonstrated that Protein L chromatography effectively separated various byproducts generated during the production of bispecific antibodies (bsAbs), including half-antibody, homodimer, LC-missing species, and aggregates. Cytiva recently launched its second-generation Protein L resin, MabSelect VL, which offers significantly improved binding capacity compared to its predecessor, Capto L.</p><p><strong>Objective: </strong>This study aimed to explore the feasibility of developing a two-column process, which includes MabSelect VL capture step and a polishing step, for purification of complex antibody molecules.</p><p><strong>Methods: </strong>We employed two bsAb cases to demonstrate that MabSelect VL's enhanced byproduct removal capability allows for a potential two-column purification process.</p><p><strong>Results: </strong>For both bsAbs, the developed two-column process yielded a product with quality attributes comparable to those obtained using the traditional three-column process.</p><p><strong>Conclusion: </strong>The MabSelect VL-based two-column process can be successfully applied to bsAb purification. In addition, it should also be feasible with regular monoclonal antibodies, whose purification is generally less challenging than that of bsAbs. By reducing the downstream process from three columns to two columns, significant savings in terms of time, labor, and materials can be achieved.</p>","PeriodicalId":73618,"journal":{"name":"Journal of biological methods","volume":"12 1","pages":"e99010045"},"PeriodicalIF":0.0,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11973047/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143813136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
An organotypic model for investigating drug-radiation responses in the lung. 用于研究肺部药物辐射反应的器官型模型。
Journal of biological methods Pub Date : 2024-11-28 eCollection Date: 2025-01-01 DOI: 10.14440/jbm.2025.0080
Maryam Alkadhimi, Anuradha Helen Manne, Yanyan Jiang, Marcus Green, Anderson Joseph Ryan
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