JADA foundational science最新文献

{"title":"Cleaning of dental handpieces","authors":"Ludger Schnieder PhD,&nbsp;Karen Seekamp-Schnieder PhD","doi":"10.1016/j.jfscie.2023.100030","DOIUrl":"https://doi.org/10.1016/j.jfscie.2023.100030","url":null,"abstract":"<div><h3>Background</h3><p>This work aimed to provide evidence for the importance of regular cleaning and care of dental handpieces. In addition, this study aimed to show that automated cleaning of dental tools may deliver superior results compared with manual cleaning.</p></div><div><h3>Methods</h3><p>Publicly available data from the US Food and Drug Administration’s (FDA’s) Manufacturer and User Facility Device Experience (MAUDE) database on incidents in the use of dental handpieces from the past 10 years were evaluated. The cleaning results of more than 4,830 instruments were also investigated and evaluated.</p></div><div><h3>Results</h3><p>By evaluating the manufacturer’s information on the incidents reported in the FDA’s MAUDE database, the connection between inadequate or no maintenance and care and the incidents was clearly shown. The evaluation of the results of manual cleaning compared with automated cleaning clearly shows that better results can be achieved through automated cleaning.</p></div><div><h3>Conclusions</h3><p>The evaluation of the FDA’s MAUDE database confirmed and emphasized the importance of cleaning and care for the safe and long-term use of dental tools. Comparing the data from manual cleaning and the usage of a device for automated cleaning of dental handpieces, it was shown that the establishment of a quality-controlled system for in-use testing of cleaning performance could provide valuable data for assessing the efficacy of different cleaning processes. Valuable feedback for the user can be derived this way. In addition, it was shown that automated cleaning of dental handpieces is a reasonable alternative and leads to clearly better results.</p></div>","PeriodicalId":73530,"journal":{"name":"JADA foundational science","volume":"3 ","pages":"Article 100030"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772414X23000105/pdfft?md5=35a775d5aae60460cfa62da367d545d0&pid=1-s2.0-S2772414X23000105-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139999371","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of flow cytometry to analyze microbial quality of dental unit water","authors":"Clifton M. Carey PhD ,&nbsp;Shannon E. Mills DDS ,&nbsp;Richard Vigil MS ,&nbsp;Madison Aungst BS, DDS ,&nbsp;Lacey Favazzo PhD","doi":"10.1016/j.jfscie.2024.100037","DOIUrl":"10.1016/j.jfscie.2024.100037","url":null,"abstract":"<div><h3>Background</h3><div>Dental unit waterline (DUWL) safety is vital for dental practitioners. Unfortunately, the standard heterotrophic plate count (HPC) methods commonly used to identify bacterial contamination dramatically underestimate planktonic bacteria counts. Flow cytometry (FCM) directly counts bacterial cells using laser-activated fluorescence, providing more reliable and timely results.</div></div><div><h3>Methods</h3><div>Approximately 1,700 DUWL samples were measured by both HPC and FCM methods. HPC samples were plated on R2A agar within 8 hours of receipt, incubated at 25 °C for 7 days, and reported as colony-forming units (CFU)/mL. FCM samples were treated with SYBR Green I fluorescent stain, analyzed and reported as high nucleic acid (HNA) and low nucleic acid (LNA) cell counts. An empirical algorithm was developed using HNA and LNA cell counts compared with the recommended action limit for the Centers for Disease Control and Prevention’s greater than 500 CFU/mL pass or fail.</div></div><div><h3>Results</h3><div>An FCM threshold limit that included both HNA 250 or greater and LNA 750 or greater cell counts was derived by pairing FCM data with R2A samples dichotomized as passing (≤ 500 CFU/mL) or failing (&gt; 500 CFU/mL) to establish corresponding pass or fail criteria that would minimize false pass and false fail compared with R2A results. 1,388 (81.6%) of samples passed the R2A limit of 500 CFU/mL or fewer, whereas 1,427 (83.9%) FCM samples passed the proposed HNA 250 or fewer and LNA 750 or fewer criteria.</div></div><div><h3>Conclusions</h3><div>The data show that FCM analysis and the proposed threshold criteria can be used as a powerful adjunctive tool for monitoring DUWL contamination, which includes greater accuracy and the ability to analyze samples immediately on receipt.</div></div>","PeriodicalId":73530,"journal":{"name":"JADA foundational science","volume":"3 ","pages":"Article 100037"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142326573","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Disruptive innovation events in dentistry","authors":"Jill Sirko PhD,&nbsp;Wenyuan Shi PhD","doi":"10.1016/j.jfscie.2024.100036","DOIUrl":"10.1016/j.jfscie.2024.100036","url":null,"abstract":"","PeriodicalId":73530,"journal":{"name":"JADA foundational science","volume":"3 ","pages":"Article 100036"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142573007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of scaling and root planing with and without minocycline hydrochloride microspheres on serum biomarkers and acute phase reactants","authors":"Michelle C. Arnett MS, RDH ,&nbsp;Massimo Costalonga DMD, PhD ,&nbsp;Phonsuda Chanthavisouk MDT, BSDH ,&nbsp;Michael D. Evans MS ,&nbsp;Danna R. Paulson MSDH, RDH, CCRP","doi":"10.1016/j.jfscie.2024.100040","DOIUrl":"10.1016/j.jfscie.2024.100040","url":null,"abstract":"<div><h3>Background</h3><div>This study tests the effects of scaling and root planing (SRP) vs SRP with minocycline hydrochloride microspheres (MMs) (SRP with MM) on serum biomarkers interleukin (IL)-1β, IL-6, tumor necrosis factor-α, and matrix metalloproteinase-8 and acute phase reactants hemoglobin A_1c (HbA_1c), high-sensitivity C-reactive proteins and haptoglobin (Hp) in patients with stage II-IV grade B periodontitis.</div></div><div><h3>Methods</h3><div>Seventy participants were randomized to receive SRP (n = 35) or SRP+MM (n = 35). Serum was collected at baseline (before SRP), 1-month reevaluation visit, and 3- and 6-month periodontal maintenance visits. MMs were delivered to pockets 5 mm or larger immediately after SRP and immediately after the 3-month periodontal maintenance visit. Serum for acute phase reactants only was collected at the 9- and 12-month posttreatment follow-up. All outcomes were summarized using estimated marginal means back-transformed to the original response scale with 95% CIs.</div></div><div><h3>Results</h3><div>At 6 months, no statistical significance was yielded in either group for IL-6 (<em>P</em> = .91), tumor necrosis factor-α (<em>P</em> = .34), or matrix metalloproteinase-8 (<em>P</em> = .34). IL-1β (<em>P</em> = .06) was slightly higher in the SRP-alone group, suggesting a clinical impact with the addition of MM. Acute phase reactants were not statistically significant for high-sensitivity C-reactive proteins (<em>P</em> = .59), HbA_1c (<em>P</em> = .46), or haptoglobin (<em>P</em> = .22) for either group. These outcomes continued at the 9- and 12-month posttreatment follow-up.</div></div><div><h3>Conclusions</h3><div>SRP alone and SRP+MM minimally reduced levels of cytokine biomarkers and acute phase reactants in self-reported systemically healthy patients with advanced stages of periodontitis. Thresholds for resolution of local clinical inflammation may not have been achieved in this study to result in a reduction of systemic inflammation.</div></div>","PeriodicalId":73530,"journal":{"name":"JADA foundational science","volume":"3 ","pages":"Article 100040"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142654050","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Detection of human papillomavirus infection in oral mucosal diseases","authors":"Paolo Junior Fantozzi DDS ,&nbsp;Umberto Romeo DDS ,&nbsp;Gianluca Tenore DDS, PhD ,&nbsp;Gaspare Palaia DDS, PhD ,&nbsp;Chiara Ciolfi DDS ,&nbsp;Alessandra Pierangeli PhD ,&nbsp;Cira Rosaria Tiziana Di Gioia MD, PhD ,&nbsp;Alessandro Villa DDS, PhD, MPH","doi":"10.1016/j.jfscie.2024.100031","DOIUrl":"https://doi.org/10.1016/j.jfscie.2024.100031","url":null,"abstract":"<div><h3>Background</h3><p>Human papillomavirus (HPV) infection accounts for more than 70% of oropharyngeal cancers but only a small proportion of oral cavity cancers. This study aimed to investigate the presence of HPV DNA in oral diseases to understand better the possible correlation between oral lesions and HPV infections.</p></div><div><h3>Methods</h3><p>This was a cross-sectional study of 99 adult patients seen for the evaluation of oral diseases. All patients received an oral biopsy and histopathologic examination and a brush biopsy for HPV-DNA detection and genotyping by real-time polymerase chain reaction. Immunohistochemistry was used to assess p16INK4a expression.</p></div><div><h3>Results</h3><p>HPV was identified in 15 of 99 (15.2%) patients (males, 66.6%). Patients with oral leukoplakia (OL) (46.6%), followed by patients with oral lichen planus (OLP) (33.3%) had the highest rate of HPV infection, with a predilection for the buccal mucosa (17.5%). Most patients with high-risk HPV infections had OLP (4/10, 40.0%), whereas most of the patients with low-risk HPV infections had nonreactive epithelial hyperkeratosis (3/6, 50.0%). Among all benign lesions, 19.0% were positive for any HPV infection. One patient with OL showing mild epithelial dysplasia had a positive p16INK4a expression.</p></div><div><h3>Conclusions</h3><p>The highest rate of HPV infection was in male patients, patients with OL and OLP, and conditions affecting the buccal mucosa. Larger studies are needed to elucidate the role of HPV in the development of these conditions.</p></div>","PeriodicalId":73530,"journal":{"name":"JADA foundational science","volume":"3 ","pages":"Article 100031"},"PeriodicalIF":0.0,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2772414X2400001X/pdfft?md5=4e7645c8b5dbc1b14aae1d459d4c1171&pid=1-s2.0-S2772414X2400001X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140555056","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Show me \"Bioactive!\": Let's play the Feud.","authors":"Jack L Ferracane","doi":"10.1016/j.jfscie.2023.100027","DOIUrl":"10.1016/j.jfscie.2023.100027","url":null,"abstract":"","PeriodicalId":73530,"journal":{"name":"JADA foundational science","volume":"1 1","pages":"100027"},"PeriodicalIF":0.0,"publicationDate":"2023-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13229046/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54466378","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Analysis of the transparent surface layer formed at the surfaces of arrested enamel caries lesions with tomographic imaging methods.","authors":"Nai-Yuan N Chang, Morgan Ng, Tina Dillas, Yi-Ching Ho, Yihua Zhu, Daniel Fried","doi":"10.1016/j.jfscie.2023.100025","DOIUrl":"10.1016/j.jfscie.2023.100025","url":null,"abstract":"<p><strong>Background: </strong>Optical coherence tomography (OCT) can acquire high-resolution images of lesion structure in vivo to assess lesion activity. The surface layers of arrested enamel caries lesions (ECLs) have a higher mineral content than the lesion body with a reduced porosity that inhibits the penetration of fluids into the lesion and appears with reduced reflectivity OCT. This study aimed to use microcomputed tomography (microCT) to measure the mineral content of the transparent surface layer (TSL) that appears on OCT images.</p><p><strong>Methods: </strong>OCT and microCT were used to measure the reflectivity and relative mineral density profiles of 20 bovine enamel samples that had simulated arrested ECLs, 44 extracted teeth with 17 proximal ECLs, 10 occlusal ECLs, and 17 lesions caused by fluorosis. TSL thickness (OCT), surface layer thickness (microCT), and the minimum and maximum densities of the TSLs were determined from matched OCT and microCT profiles.</p><p><strong>Results: </strong>For all samples, the TSL thickness measured with OCT was significantly less than the surface layer thickness measured with microCT (<i>P</i> < .05). Mean maximum mineral densities in the TSL were statistically similar to sound human enamel (<i>P</i> > .05) and significantly higher (<i>P</i> < .05) than the mean minimal mineral densities of the TSL.</p><p><strong>Conclusions: </strong>The maximum mineral densities of the TSL measurable with OCT approach those of sound enamel suggesting that almost complete remineralization has occurred in the TSLs. This study provides further confirmation that the presence of a TSL in OCT images is a key indicator of lesion arrest.</p>","PeriodicalId":73530,"journal":{"name":"JADA foundational science","volume":"1 1","pages":"100025"},"PeriodicalIF":0.0,"publicationDate":"2023-06-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13229035/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54466355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ease of use in accessing electronic dental records with a touchless interface compared with a conventional mouse.","authors":"Tudor Ilies, Nicholas Camic, Aditya Tadinada","doi":"10.1016/j.jfscie.2023.100024","DOIUrl":"10.1016/j.jfscie.2023.100024","url":null,"abstract":"","PeriodicalId":73530,"journal":{"name":"JADA foundational science","volume":"21 1","pages":"100024"},"PeriodicalIF":0.0,"publicationDate":"2023-06-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13229028/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54466347","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bioactive dental materials: Developing, promising, confusing.","authors":"Jack L Ferracane, Sharanbir K Sidhu, Mary Anne S Melo, In-Sung Luke Yeo, Anibal Diogenes, Brian W Darvell","doi":"10.1016/j.jfscie.2023.100022","DOIUrl":"10.1016/j.jfscie.2023.100022","url":null,"abstract":"","PeriodicalId":73530,"journal":{"name":"JADA foundational science","volume":"1 1","pages":"100022"},"PeriodicalIF":0.0,"publicationDate":"2023-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13229067/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54466333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The growth and dissolution of sodium fluoride hopper crystals.","authors":"Alexis N Frigyes, Jacqueline Holdren, William A McHale, Mark A Latta, Stephen M Gross","doi":"10.1016/j.jfscie.2022.100020","DOIUrl":"10.1016/j.jfscie.2022.100020","url":null,"abstract":"<p><strong>Background: </strong>Caries is still prevalent despite the current understanding and use of fluoride. Faster-dissolving crystals of sodium fluoride (NaF) in a fluoride treatment could improve the efficacy of current preventative approaches.</p><p><strong>Methods: </strong>In this study, crystals of NaF with a high surface area-to-volume ratio were prepared by adding an aqueous solution of NaF to an ethanol-water antisolvent mixture. Dissolution of these high surface area to volume crystals was measured using a conductivity probe.</p><p><strong>Results: </strong>Key variables in the preparation of hopper crystals included the amount of water in the antisolvent, the ratio of NaF solution to antisolvent, and the rate of stirring. The dissolution rate of these crystals was significantly greater than NaF crystals produced without a hopper morphology.</p><p><strong>Conclusions: </strong>Including NaF hopper crystals in fluoride treatments could increase the bioavailability of fluoride from a fluoride treatment and potentially improve the remineralization of enamel.</p>","PeriodicalId":73530,"journal":{"name":"JADA foundational science","volume":"1 1","pages":"100020"},"PeriodicalIF":0.0,"publicationDate":"2023-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13229025/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"54466316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}