ISME communications最新文献_第8页

Time of first contact determines cooperator success in a three-member microbial consortium. 在一个三人微生物联盟中，首次接触的时间决定合作者的成功。

IF 5.1

ISME communications Pub Date : 2025-01-13 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycaf004

Rachel Los, Tobias Fecker, P A M van Touw, Rinke J van Tatenhove-Pel, Timon Idema

{"title":"Time of first contact determines cooperator success in a three-member microbial consortium.","authors":"Rachel Los, Tobias Fecker, P A M van Touw, Rinke J van Tatenhove-Pel, Timon Idema","doi":"10.1093/ismeco/ycaf004","DOIUrl":"10.1093/ismeco/ycaf004","url":null,"abstract":"Microbial communities are characterized by complex interaction, including cooperation and cheating, which have significant ecological and applied implications. However, the factors determining the success of cooperators in the presence of cheaters remain poorly understood. Here, we investigate the dynamics of cooperative interactions in a consortium consisting of a cross-feeding pair and a cheater strain using individual-based simulations and an engineered L. cremoris toy consortium. Our simulations reveal first contact time between cooperators as a critical predictor for cooperator success. By manipulating the relative distances between cooperators and cheaters or the background growth rates, influenced by the cost of cooperation, we can modulate this first contact time and influence cooperator success. Our study underscores the importance of cooperators coming into contact with each other on time, which provides a simple and generalizable framework for understanding and designing cooperative interactions in microbial communities. These findings contribute to our understanding of cross-feeding dynamics and offer practical insights for synthetic and biotechnological applications.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycaf004"},"PeriodicalIF":5.1,"publicationDate":"2025-01-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879117/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143558435","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Decrypting the phylogeny and metabolism of microbial dark matter in green and red Antarctic snow. 解密南极绿色和红色雪中微生物暗物质的系统发育和代谢。

IF 5.1

ISME communications Pub Date : 2025-01-10 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycaf003

Ze Ren, Wei Luo, Huirong Li, Haitao Ding, Yunlin Zhang

{"title":"Decrypting the phylogeny and metabolism of microbial dark matter in green and red Antarctic snow.","authors":"Ze Ren, Wei Luo, Huirong Li, Haitao Ding, Yunlin Zhang","doi":"10.1093/ismeco/ycaf003","DOIUrl":"10.1093/ismeco/ycaf003","url":null,"abstract":"Antarctic snow harbors diverse microorganisms, including pigmented algae and bacteria, which create colored snow patches and influence global climate and biogeochemical cycles. However, the genomic diversity and metabolic potential of colored snow remain poorly understood. We conducted a genome-resolved study of microbiomes in colored snow from 13 patches (7 green and 6 red) on the Fildes Peninsula, Antarctica. Using metagenome assembly and binning, we reconstructed 223 metagenome-assembled genomes (MAGs), with 91% representing previously unexplored microbes. Green snow (GS) and red snow (RS) showed distinct MAGs profile, with Polaromonas and Ferruginibacter as the most abundant genera, respectively. GS exhibited higher alpha diversity with more unique and enriched MAGs, while RS showed greater variability with higher beta diversity. All MAGs contained genes encoding auxiliary activities (AAs), carbohydrate esterases (CEs), glycoside hydrolases (GHs), and glycosyl transferases (GTs), indicating microbial degradation of complex carbon substrates. The most abundant enzymes included GT2 (cellulose synthase), GT4 (sucrose synthase), CE1 (acetyl xylan esterase), GT41 (peptide beta-N-acetylglucosaminyltransferase), and CE10 (arylesterase). GS had a higher abundance of GTs, whereas RS was enriched in GHs. Furthermore, 56% of MAGs contained genes for inorganic nitrogen cycling, with 18 gene families involved in assimilatory nitrate reduction, dissimilatory nitrate reduction, and denitrification. Potential coupling of nitrogen cycling and carbohydrate metabolism was observed at both genome and community levels, suggesting close links between these pathways, particularly through nitrate reduction during carbohydrate degradation. This study enhances our understanding of microbial metabolic functions in polar ecosystems and highlights their roles in maintaining Antarctic ecological stability.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycaf003"},"PeriodicalIF":5.1,"publicationDate":"2025-01-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11765414/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143049174","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contrasting diversity patterns between microeukaryotic and prokaryotic communities in cold-seep sediments. 冷渗沉积物中微真核生物与原核生物群落多样性格局对比。

IF 5.1

ISME communications Pub Date : 2025-01-08 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycaf002

Zhimeng Xu, Jiawei Chen, Wenzhao Liang, Zhao Liang Chen, Wenxue Wu, Xiaomin Xia, Bingzhang Chen, Ding He, Hongbin Liu

{"title":"Contrasting diversity patterns between microeukaryotic and prokaryotic communities in cold-seep sediments.","authors":"Zhimeng Xu, Jiawei Chen, Wenzhao Liang, Zhao Liang Chen, Wenxue Wu, Xiaomin Xia, Bingzhang Chen, Ding He, Hongbin Liu","doi":"10.1093/ismeco/ycaf002","DOIUrl":"10.1093/ismeco/ycaf002","url":null,"abstract":"Cold seeps are hotspots of biodiversity. However, the quantification of the microbial diversity, particularly that of microeukaryotes, remains scarce and little is known about the active groups. In this study we investigated the diversity and activity of prokaryotes and microeukaryotes in the Haima cold seep sediments in the northern South China Sea using both DNA (whole community) and RNA (active community) signatures. We found that, in general, prokaryotes had lower diversity in the seep sediment than in non-seep regions while microeukaryotes showed the opposite pattern. This finding could be explained by the dominance of homogeneous selection in the prokaryotic community while microeukaryotic communities were less affected by environmental selection, harboring high richness of abundant groups in the seep regions. The compositional difference between DNA and RNA communities was much larger in microeukaryotes than prokaryotes, which could be reflected by the large number of inactive microeukaryotic taxa. Compared to the whole community, the seep-active groups, e.g. among microeukaryotes, Breviatea, Labyrinthulomycetes, and Apicomplexa were more sensitive to and directly influenced by environmental factors, suggesting their pivotal roles in ecosystem biodiversity and functions. This study provides insight into the distinct diversity patterns and regulating mechanisms that occur between prokaryotic and microeukaryotic communities in cold-seep sediments, deepening our understanding of microbial ecology in deep-sea extreme habitats.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycaf002"},"PeriodicalIF":5.1,"publicationDate":"2025-01-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11879339/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143560290","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Sexual reproduction during diatom bloom. 硅藻华期间有性繁殖。

IF 5.1

ISME communications Pub Date : 2025-01-07 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycae169

Léa Prigent, Julien Quéré, Martin Plus, Mickael Le Gac

{"title":"Sexual reproduction during diatom bloom.","authors":"Léa Prigent, Julien Quéré, Martin Plus, Mickael Le Gac","doi":"10.1093/ismeco/ycae169","DOIUrl":"10.1093/ismeco/ycae169","url":null,"abstract":"Phytoplankton supports food webs in all aquatic ecosystems. Ecological studies highlighted the links between environmental variables and species successions in situ. However, the role of life cycle characteristics on phytoplankton community dynamics remains poorly characterized. In diatoms, sexual reproduction creates new genetic combinations and prevents excessive cell size miniaturization. It has been extensively studied in vitro but seldom in the natural environment. Here, analyzing metatranscriptomic data in the light of the expression patterns previously characterized in vitro, we identified a synchronized and transient sexual reproduction event during a bloom of the toxic diatom species Pseudo-nitzschia australis. Despite the complexity of environmental conditions encountered in situ, sexual reproduction appeared to be the strongest differential gene expression signal that occurred during the bloom. The potential link between environmental conditions and the initiation of sexual reproduction remain to be determined, but sexual reproduction probably had a major impact on the bloom dynamic.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycae169"},"PeriodicalIF":5.1,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11749564/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017585","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Marine phytoplankton impose strong selective pressures on in vitro microbiome assembly, but drift is the dominant process. 海洋浮游植物对体外微生物组的组装施加了强大的选择压力，但漂移是主要的过程。

IF 5.1

ISME communications Pub Date : 2025-01-06 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycaf001

Sergio E Morales, Sven P Tobias-Hünefeldt, Evelyn Armstrong, William S Pearman, Kirill Bogdanov

{"title":"Marine phytoplankton impose strong selective pressures on in vitro microbiome assembly, but drift is the dominant process.","authors":"Sergio E Morales, Sven P Tobias-Hünefeldt, Evelyn Armstrong, William S Pearman, Kirill Bogdanov","doi":"10.1093/ismeco/ycaf001","DOIUrl":"10.1093/ismeco/ycaf001","url":null,"abstract":"Phytoplankton are known ecosystem engineers that modulate ocean community assembly processes, but the universality and extent of their microbiome control remains unclear. We used in vitro incubations and 16S ribosomal RNA gene amplicon sequencing to test the influence of Southern and South Pacific oceans dominant phytoplankton on assembly processes and community successions in response to phytoplankton blooms. Phytoplankton grown with reduced-diversity cultures or supplemented with exogenously added microbiomes showed reduced diversity, suggesting environmental filtering. Community profiles were distinct under all culture conditions, further confirming strong selection for specific microbiomes based on phytoplankton. Analysis of core, abundant, and rare organisms in each culture condition showed a conserved response in which core organisms were enriched under conditions of exogenously added phytoplankton. Progression through phytoplankton growth phases selected first for rare and abundant organisms, with increased selection for core members during the exponential phase and relaxing of selection during the death phase, as seen throughout incubations for microbiome-only controls. Surprisingly, selection process quantification identified drift as the dominant process across all conditions and growth phases, with homogenous selection and dispersal limitation accounting for the remainder. Altogether, using Southern Ocean-derived model organisms we confirmed the role phytoplankton play in community assembly but also demonstrated that stochastic processes still predominately drive community selection.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycaf001"},"PeriodicalIF":5.1,"publicationDate":"2025-01-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11843096/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143484837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Soil microbial carbon use efficiency differs between mycorrhizal trees: insights from substrate stoichiometry and microbial networks. 菌根树的土壤微生物碳利用效率不同：来自基质化学计量学和微生物网络的见解。

IF 5.1

ISME communications Pub Date : 2024-12-27 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycae173

Jing Yu, Jingyi Yang, Lingrui Qu, Xiaoyi Huang, Yue Liu, Ping Jiang, Chao Wang

{"title":"Soil microbial carbon use efficiency differs between mycorrhizal trees: insights from substrate stoichiometry and microbial networks.","authors":"Jing Yu, Jingyi Yang, Lingrui Qu, Xiaoyi Huang, Yue Liu, Ping Jiang, Chao Wang","doi":"10.1093/ismeco/ycae173","DOIUrl":"10.1093/ismeco/ycae173","url":null,"abstract":"The role of mycorrhizal associations in controlling forest soil carbon storage remains under debate. This uncertainty is potentially due to an incomplete understanding of their influence on the free-living soil microbiome and its functions. In this study, rhizosphere and non-rhizosphere soils were collected from eight arbuscular mycorrhizal (AM) and seven ectomycorrhizal (ECM) tree species in a temperate forest. We employed high-throughput sequencing and 18O-H2O labeling to analyze the soil microbial community and carbon use efficiency (CUE), respectively. We find microbial respiration rates are higher in rhizosphere than that in non-rhizosphere soils for ECM trees, whereas microbial growth rates show no significant differences. Consequently, microbial CUE is lower in rhizosphere compared to non-rhizosphere soils for ECM trees. In addition, we find that non-rhizosphere soils from ECM trees exhibited higher CUE compared to those from AM trees. Furthermore, we observe that bacterial-fungal co-occurrence networks in ECM soils exhibit greater complexity relative to AM ones. Using random forest and structural equation modeling analyses, we find that microbial stoichiometric carbon/nitrogen imbalance and network complexity are key predictors of soil microbial CUE for AM and ECM trees, respectively. Our findings shed new light on the pivotal role of mycorrhizal associations in shaping free-living microbial communities and their metabolic characteristics in the studied soils. These insights are critical for predicting soil carbon sequestration in response to shifts in ECM and AM species within temperate forest under climate change.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycae173"},"PeriodicalIF":5.1,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arsenic modifies the microbial community assembly of soil-root habitats in Pteris vittata. 砷改变了蜈蚣草土壤根生境的微生物群落组合。

IF 5.1

ISME communications Pub Date : 2024-12-27 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycae172

Jiahui Lin, Zhongmin Dai, Mei Lei, Qian Qi, Weijun Zhou, Lena Q Ma, Randy A Dahlgren, Jianming Xu

{"title":"Arsenic modifies the microbial community assembly of soil-root habitats in Pteris vittata.","authors":"Jiahui Lin, Zhongmin Dai, Mei Lei, Qian Qi, Weijun Zhou, Lena Q Ma, Randy A Dahlgren, Jianming Xu","doi":"10.1093/ismeco/ycae172","DOIUrl":"10.1093/ismeco/ycae172","url":null,"abstract":"Pteris vittata, renowned for its ability to hyperaccumulate arsenic, presents a promising solution to the escalating issue of global soil arsenic contamination. This fern cultivates a unique underground microbial community to enhance its environmental adaptability. However, our understanding of the assembly process and the long-term ecological impacts of this community remains limited, hindering the development of effective soil remediation strategies. This study addresses this gap by investigating soil-root habitats from three geographically diverse fields comprising a gradient of arsenic contamination, complemented by a time-scale greenhouse experiment. Field investigations reveal that arsenic stress influences community assembly dynamics in the rhizosphere by enhancing processes of homogeneous selection. Greenhouse experiments further reveal that arsenic exposure alters the assembly trajectory of rhizosphere communities by promoting key microbial modules. Specifically, arsenic exposure increases the enrichment of a core taxon (i.e. Rhizobiaceae) in the rhizosphere, both in field and greenhouse settings, boosting their abundance from undetectable levels to 0.02% in the soil after phytoremediation. Notably, arsenic exposure also promotes a pathogenic group (i.e. Spirochaetaceae) in the rhizosphere, increasing their abundance from undetectable levels to 0.1% in the greenhouse. This raise concerns that warrant further investigation in future phytoremediation studies. Overall, this study elucidates the assembly dynamics of the soil microbiome following the introduction of a remediation plant and emphasizes the often-overlooked impacts on soil microbial community following phytoremediation. By probing the ecological impacts of remediation plants, this work advances a more nuanced understanding of the complex ecological implications inherent in phytoremediation processes.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycae172"},"PeriodicalIF":5.1,"publicationDate":"2024-12-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11742257/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Litter quality modulates changes in bacterial and fungal communities during the gut transit of earthworm species of different ecological groups. 凋落物质量调节不同生态类群蚯蚓肠道运输过程中细菌和真菌群落的变化。

IF 5.1

ISME communications Pub Date : 2024-12-26 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycae171

Huizhen Chao, Linlin Zhong, Ina Schaefer, Mingming Sun, André Junggebauer, Feng Hu, Stefan Scheu

{"title":"Litter quality modulates changes in bacterial and fungal communities during the gut transit of earthworm species of different ecological groups.","authors":"Huizhen Chao, Linlin Zhong, Ina Schaefer, Mingming Sun, André Junggebauer, Feng Hu, Stefan Scheu","doi":"10.1093/ismeco/ycae171","DOIUrl":"10.1093/ismeco/ycae171","url":null,"abstract":"Earthworms are keystone animals stimulating litter decomposition and nutrient cycling. However, earthworms comprise diverse species which live in different soil layers and consume different types of food. Microorganisms in the gut of earthworms are likely to contribute significantly to their ability to digest organic matter, but this may vary among earthworm species. Here, we analyse the effect of food (litter) quality on gut microbiota and their changes during the gut passage (from foregut to hindgut) of earthworms of different ecological groups. The endogeic (soil living) species Aporrectodea caliginosa and the anecic (litter feeding) species Lumbricus terrestris were fed with high- (rape leaves) and low-quality litter (wheat straw) in a microcosm experiment for 18 weeks. Irrespective of earthworm species, alpha diversity of bacterial and fungal communities changed little during the gut passage, with the composition and diversity of microbial communities in the gut generally resembling those in soil more than in litter. In addition, the low-quality litter supported higher alpha diversity and more complex communities than high-quality litter. Further, gut microbial communities of the anecic L. terrestris changed less during gut passage than those of the endogeic A. caliginosa, especially when fed low-quality litter. Our findings indicate that earthworm gut microbial communities are predominantly shaped by the soil they ingest, but are modulated by the quality of litter they feed on and earthworm ecological group. Overall, the results suggest that earthworms primarily influence soil microbiota by mixing and spreading microorganisms from different microhabitats through bioturbation rather than by digesting microorganisms.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycae171"},"PeriodicalIF":5.1,"publicationDate":"2024-12-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11778916/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143070062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microcystin shapes the Microcystis phycosphere through community filtering and by influencing cross-feeding interactions. 微囊藻毒素通过群落过滤和影响交叉摄食相互作用来塑造微囊藻的藻圈。

IF 5.1

ISME communications Pub Date : 2024-12-24 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycae170

Rebecca Große, Markus Heuser, Jonna E Teikari, Dinesh K Ramakrishnan, Ahmed Abdelfattah, Elke Dittmann

{"title":"Microcystin shapes the Microcystis phycosphere through community filtering and by influencing cross-feeding interactions.","authors":"Rebecca Große, Markus Heuser, Jonna E Teikari, Dinesh K Ramakrishnan, Ahmed Abdelfattah, Elke Dittmann","doi":"10.1093/ismeco/ycae170","DOIUrl":"10.1093/ismeco/ycae170","url":null,"abstract":"The cyanobacterium Microcystis causes harmful algal blooms that pose a major threat to human health and ecosystem services, particularly due to the prevalence of the potent hepatotoxin microcystin (MC). With their pronounced EPS layer, Microcystis colonies also serve as a hub for heterotrophic phycosphere bacteria. Here, we tested the hypothesis that the genotypic plasticity in its ability to produce MC influences the composition and assembly of the Microcystis phycosphere microbiome. In an analysis of individual colonies of a natural Microcystis bloom, we observed a significantly reduced richness of the community in the presence of MC biosynthesis genes. A subsequent synthetic community experiment with 21 heterotrophic bacterial strains in co-cultivation with either the wild-type strain Microcystis aeruginosa PCC 7806 or the MC-free mutant ΔmcyB revealed not only a tug-of-war between phototrophic and heterotrophic bacteria, but also a reciprocal dominance of two isolates of the genus Sphingomonas and Flavobacterium. In contrast, an Agrobacterium isolate thrived equally well in both consortia. In substrate utilization tests, Sphingomonas showed the strongest dependence on Microcystis exudates with a clear preference for the wild-type strain. Genome sequencing revealed a high potential for complementary cross-feeding, particularly for the Agrobacterium and Sphingomonas isolates but no potential for MC degradation. We postulate that strain-specific functional traits, such as the ability to perform glycolate oxidation, play a crucial role in the cross-feeding interactions, and that MC is one of the determining factors in the Microcystis phycosphere due to its interference with inorganic carbon metabolism.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycae170"},"PeriodicalIF":5.1,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748430/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017584","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Influences of fluctuating nutrient loadings on nitrate-reducing microorganisms in rivers. 波动的营养负荷对河流中硝酸盐还原微生物的影响。

IF 5.1

ISME communications Pub Date : 2024-12-24 eCollection Date: 2025-01-01 DOI: 10.1093/ismeco/ycae168

Shengjie Li, Rui Zhao, Shuo Wang, Yiwen Yang, Muhe Diao, Guodong Ji

{"title":"Influences of fluctuating nutrient loadings on nitrate-reducing microorganisms in rivers.","authors":"Shengjie Li, Rui Zhao, Shuo Wang, Yiwen Yang, Muhe Diao, Guodong Ji","doi":"10.1093/ismeco/ycae168","DOIUrl":"10.1093/ismeco/ycae168","url":null,"abstract":"Rivers serve important functions for human society and are significantly impacted by anthropogenic nutrient inputs (e.g. organic and sulfur compounds). Reduced organic and sulfur compounds influence the nitrogen cycle as they are electron donors of microbial nitrate reduction. Water pollution caused by individual nutrients and the mechanisms have been studied, but how the variation in multiple nutrient loadings influences nitrate-reducing microorganisms is less understood. Two sets of microcosms were established and exposed to nitrate, along with either acetate or thiosulfate, at different times. Nutrient concentrations responded to the loading pollutant. The nutrient loading order was more important in shaping microbial community structure and microbial interactions through the exchange of growth-required substances. This indicated that upstream or historical nutrient inflows impacted current nitrate reduction by changing the seeding microbial community, highlighting the importance of river connectivity. Based on metatranscriptome analysis, although the order and type of nutrient loadings were equally important in regulating global transcriptomes, transcripts of enzymes for key metabolisms (nitrate reduction, sulfur oxidation, etc.) more actively responded to the nutrient type. The regulation of a small set of genes was sufficient to make the transition, while most transcripts were not degraded and regenerated. These insights are important for understanding the varying pollution status of rivers and for developing effective solutions, such as remediation.","PeriodicalId":73516,"journal":{"name":"ISME communications","volume":"5 1","pages":"ycae168"},"PeriodicalIF":5.1,"publicationDate":"2024-12-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11748280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017582","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0