IJC metabolic & endocrine最新文献

{"title":"Aging impairs ischemia-induced neovascularization by attenuating the mobilization of bone marrow-derived angiogenic cells","authors":"Yuen Ting Lam ,&nbsp;Laura Lecce ,&nbsp;Zoe Clayton ,&nbsp;Philippa J.L. Simpson ,&nbsp;Richard H. Karas ,&nbsp;Martin K.C. Ng","doi":"10.1016/j.ijcme.2016.05.005","DOIUrl":"10.1016/j.ijcme.2016.05.005","url":null,"abstract":"<div><h3>Background</h3><p>Aging is associated with impaired ischemia-induced neovascularization. However, the effects of aging on bone marrow-derived angiogenic cell (BMDAC)-mediated vasculogenesis and on angiogenesis at the ischemic sites remain incompletely understood.</p></div><div><h3>Methods and results</h3><p>Two- and 24-month old male C57Bl/6J mice were subjected to hindlimb ischemia. The levels of Sca1<!--> <!-->+/CXCR4<!--> <!-->+ BMDACs were determined post-ischemia by flow cytometry. In young mice, ischemia increased Sca1<!--> <!-->+/CXCR4<!--> <!-->+ BMDAC levels in the bone marrow and spleen at day 3 (p<!--> <!-->&lt;<!--> <!-->0.001) and in the circulating blood at day 7 (p<!--> <!-->&lt;<!--> <!-->0.01) post-ischemia. However, ischemia-induced elevation of progenitor cells was attenuated in the bone marrow, spleen and blood of old mice despite a preserved HIF-1α-mediated angiogenic response in the ischemic tissues. Irradiated young recipient mice engrafted with old bone marrow displayed reduced levels of Sca1<!--> <!-->+/CXCR4<!--> <!-->+ BMDACs in the bone marrow and circulating blood post-ischemia compared to recipients with young bone marrow. <em>Ex vivo</em> cultured BMDACs from old mice exhibited reduced SDF-1-stimulated migration (p<!--> <!-->&lt;<!--> <!-->0.01) and a decrease in JAK-2 and AKT activation. However, the intrinsic angiogenic function of BMDACs, including VEGF secretion and promotion of endothelial cell tubule formation, was preserved with aging. Furthermore, facilitated mobilization of old bone marrow-derived mononuclear cells to the ischemic hindlimb by intramuscular injection enhanced ischemia-induced neovascularization in old mice <em>in vivo</em> (p<!--> <!-->&lt;<!--> <!-->0.001).</p></div><div><h3>Conclusions</h3><p>The age-related impairment in ischemia-induced neovascularization is largely attributable to a marked attenuation of BMDAC mobilization with a preservation of intrinsic angiogenic function with age.</p></div>","PeriodicalId":73333,"journal":{"name":"IJC metabolic & endocrine","volume":"12 ","pages":"Pages 19-29"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcme.2016.05.005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85294863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Soluble urokinase plasminogen activator receptor (suPAR): Its relation to neurological outcome in patients with survived cardiac arrest","authors":"Obaida R. Rana ,&nbsp;Jörg W. Schröder ,&nbsp;Alexander Koch ,&nbsp;Frank Tacke ,&nbsp;Ralf Koos ,&nbsp;Robert H.G. Schwinger ,&nbsp;Malte Kelm ,&nbsp;Samir M. Said ,&nbsp;Erol Saygili","doi":"10.1016/j.ijcme.2016.05.008","DOIUrl":"10.1016/j.ijcme.2016.05.008","url":null,"abstract":"<div><h3>Background</h3><p>High serum levels of the inflammatory biomarker soluble urokinase plasminogen activator receptor (suPAR) have been associated with poor neurological outcome in patients after cardiac arrest (CA), but with inadequate and contradictive prediction values. The purpose of this study was to provide further evidence on the prognostic value of suPAR for the prediction of poor neurological outcome after initially survived CA.</p></div><div><h3>Methods</h3><p>A total of 177 patients were prospectively enrolled in this cohort study. 85 patients with survived CA were included and the neurological outcome was assessed after 6<!--> <!-->months. 71 patients with ST-segmental elevation myocardial infarction (STEMI) and 21 healthy control patients served as comparative groups.</p></div><div><h3>Results</h3><p>The serum suPAR levels on admission and the subsequent serum course were significantly higher in patients with CA as compared to STEMI and control patients. Furthermore, patients with poor neurological outcome showed significantly higher serum suPAR levels as compared to patients with good neurological outcome. By the use of ROC-curves and setting the specificities to 100%, inadequate sensitivities and cut-off values were calculated (day 2: sensitivity 21.1%, cut-off 10.2<!--> <!-->ng/dl, AUC 0.716). By setting the specificities to at least 80% the best prediction values could be calculated for day 2 with a sensitivity of 57.9% and a cut-off value of 5.3<!--> <!-->ng/dl.</p></div><div><h3>Conclusions</h3><p>SuPAR serum levels in patients with poor neurological outcome were significantly higher as compared to patients with good neurological outcome. However, the prognostic value was low and inadequate because of a substantial overlap of serum suPAR levels between the outcome groups.</p></div>","PeriodicalId":73333,"journal":{"name":"IJC metabolic & endocrine","volume":"12 ","pages":"Pages 8-13"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcme.2016.05.008","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78100415","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cardiovascular risk assessment using LOX-index and Self-Rating Depression Scale","authors":"Nobutaka Inoue,&nbsp;Kazue Fukuyama,&nbsp;Sonoko Hirayama,&nbsp;Takayuki Yoshioka,&nbsp;Toru Ozawa,&nbsp;Sachiyo Iwata,&nbsp;Asumi Takei","doi":"10.1016/j.ijcme.2016.05.002","DOIUrl":"10.1016/j.ijcme.2016.05.002","url":null,"abstract":"<div><h3><span>Objective</span></h3><p>LOX-Index is a novel biomarker for cardiovascular disease (CVD) and is calculated by multiplying LOX-1 ligands containing apolipoprotein B (LAB) and soluble LOX-1 (sLOX-1). The Framingham risk score (FRS) is a common clinical tool for risk assessment of coronary artery disease. Mental stress can also be an important risk factor for CVD. The purpose of this study was to examine the relationship between LOX-Index and FRS or mental stress.</p></div><div><h3>Methods</h3><p>LOX-Index was measured in 453 subjects including 150 consecutive outpatients with lifestyle-related diseases such as diabetes, hyperlipidemia, and hypertension and 303 healthy volunteers. Mental stress was evaluated by the Self-Rating Depression Scale (SDS).</p></div><div><h3>Results</h3><p>LOX-Index was significantly related with the 10-years risk of FRS. Multiple regression analysis demonstrated that LAB was closely associated with the smoking status, low-density lipoprotein (LDL), and high-density lipoprotein (HDL). There were no significant associations between LOX-Index and the SDS scores; however, by simultaneously using LOX-Index and SDS, the subjects could be classified in terms of oxidative stress and mental stress.</p></div><div><h3>Conclusions</h3><p>LOX-Index appears to be a comprehensive marker that could evaluate the status of multiple CVD risk factors. The classification with LOX-Index and SDS could contribute to the risk assessment for CVD.</p></div>","PeriodicalId":73333,"journal":{"name":"IJC metabolic & endocrine","volume":"12 ","pages":"Pages 3-7"},"PeriodicalIF":0.0,"publicationDate":"2016-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcme.2016.05.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83619827","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Posttraumatic stress disorder symptoms and hypercoagulability during emergency department evaluation for acute coronary syndrome","authors":"Vy Thuy Ho ,&nbsp;Daichi Shimbo ,&nbsp;Joan Duer-Hefele ,&nbsp;William Whang ,&nbsp;Melinda Chang ,&nbsp;Donald Edmondson","doi":"10.1016/j.ijcme.2016.03.002","DOIUrl":"10.1016/j.ijcme.2016.03.002","url":null,"abstract":"","PeriodicalId":73333,"journal":{"name":"IJC metabolic & endocrine","volume":"11 ","pages":"Pages 1-2"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcme.2016.03.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"35630527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Relationship between breast arterial calcification and lipid profile, plasma atherogenic index, Castelli's risk index and atherogenic coefficient in premenopausal women","authors":"Abdulmelik Yıldız ,&nbsp;Özlem Seçen ,&nbsp;Cennet Yıldız ,&nbsp;Mehtap Çiçekçi","doi":"10.1016/j.ijcme.2016.05.003","DOIUrl":"10.1016/j.ijcme.2016.05.003","url":null,"abstract":"<div><h3>Objective</h3><p>The aim of this study was to investigate the relationship between the breast arterial calcification (BAC) and the plasma atherogenic index (PAI), atherogenic coefficient (AC), Castelli risk index-I (CRI-I) and Castelli risk index-II (CRI-II).</p></div><div><h3>Methods</h3><p>This retrospective study included 60 premenopausal women aged over 40<!--> <!-->years with BAC on mammograms and control group of 60 women without BAC. Serum glucose, triglyceride (TG), low-density lipoproteincholesterol (LDLc), high-density lipoprotein-cholesterol (HDLc), and total cholesterol (TC), levels were measured. Lipid indices were calculated using the appropriate formula.</p></div><div><h3>Results</h3><p>LDLc, non-HDLc levels were significantly higher, HDLc levels were siginificantly lower in patient group compared to the control group (p<!--> <!-->=<!--> <!-->0.007, p<!--> <!-->=<!--> <!-->0.027, and p<!--> <!-->=<!--> <!-->0.014, respectively). Patient group had significantly higher PAI, AC, CRI-I and CRI-II levels than the control group (p<!--> <!-->=<!--> <!-->0.003, p<!--> <!-->=<!--> <!-->0.002, p<!--> <!-->=<!--> <!-->0.002 and p<!--> <!-->=<!--> <!-->0.003, respectively). A significant positive correlation was found between BAC and PAI, AC, CRI-I and CRI-II (r<!--> <!-->=<!--> <!-->0.267 and p<!--> <!-->=<!--> <!-->0.003, r<!--> <!-->=<!--> <!-->0.282 and p<!--> <!-->=<!--> <!-->0.002, r<!--> <!-->=<!--> <!-->0.282 and p<!--> <!-->=<!--> <!-->0.002, r<!--> <!-->=<!--> <!-->0.271 and p<!--> <!-->=<!--> <!-->0.003, respectively). LDLc and non-HDLc were positively correlated whereas HDLc was negatively correlated with the BAC (r<!--> <!-->=<!--> <!-->0.188 and p<!--> <!-->=<!--> <!-->0.039, r<!--> <!-->=<!--> <!-->0.202 and p<!--> <!-->=<!--> <!-->0.027, r<!--> <!-->=<!--> <!-->−<!--> <!-->0.223 and p<!--> <!-->=<!--> <!-->0.014, respectively).</p></div><div><h3>Conclusion</h3><p>BAC is a valuable tool for the prediction of deranged lipid profile. Dyslipidemia, PAI, AC, CRI-I and CRI-II are risk factors for the development of atherosclerosis. Our results indicate that BAC is potentially useful tool for the detection of dyslipidemia and early atherosclerosis in premenopausal women.</p></div>","PeriodicalId":73333,"journal":{"name":"IJC metabolic & endocrine","volume":"11 ","pages":"Pages 19-22"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcme.2016.05.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75088310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The combination of nutraceutical and simvastatin enhances the effect of simvastatin alone in normalising lipid profile without side effects in patients with ischemic heart disease","authors":"Giuseppe Campolongo ,&nbsp;Costanza Valentina Riccioni ,&nbsp;Valeria Raparelli ,&nbsp;Ilaria Spoletini ,&nbsp;Giuseppe Marazzi ,&nbsp;Cristiana Vitale ,&nbsp;Maurizio Volterrani","doi":"10.1016/j.ijcme.2016.03.001","DOIUrl":"10.1016/j.ijcme.2016.03.001","url":null,"abstract":"<div><h3>Background</h3><p>hyperlipidemia is one of the most important cardiovascular risk factors. Statins at high doses are commonly prescribed to lower LDL-cholesterol, but are often poorly tolerated. In particular, muscle pain and increase of creatine phosphokinase are frequent side effects. The purpose of this study was to assess whether the addition of a nutraceutical to simvastatin may result in the achievement of the therapeutic target (LDL-cholesterol less than 70<!--> <!-->mg/dL) without side effects in patients with ischemic heart disease.</p></div><div><h3>Methods</h3><p>Sixty-four patients with ischemic heart disease treated with simvastatin 20<!--> <!-->mg who had not achieved the therapeutic target were enrolled. Patients were randomised 1:1. Patients of group A (n<!--> <!-->=<!--> <!-->32) were given simvastatin 40<!--> <!-->mg per day and patients of group B (n<!--> <!-->=<!--> <!-->32) were given simvastatin 20<!--> <!-->mg plus 2 tablets of a nutraceutical composed of bergamot, phytosterols, artichoke, vitamin C.</p></div><div><h3>Results</h3><p>After 3<!--> <!-->months, patients in both groups showed a significant reduction from baseline in total cholesterol, LDL-c and tryglicerides. However, in group A, 4 patients reported myalgia (9,7%) with an increase in creatine phosphokinase; whereas no adverse events occurred in group B.</p></div><div><h3>Conclusions</h3><p>The association of a nutraceutical and simvastatin 20<!--> <!-->mg may be a valid therapeutic option for the treatment of hyperlipidemia in patients with ischemic heart disease intolerant to statin at high doses, in the absence of side effects. Further studies are needed to clarify the mechanisms of action of nutraceuticals.</p></div>","PeriodicalId":73333,"journal":{"name":"IJC metabolic & endocrine","volume":"11 ","pages":"Pages 3-6"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcme.2016.03.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78004417","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic profiles of lipid metabolism, arterial stiffness and hemodynamics in male coronary artery disease patients","authors":"Kaido Paapstel ,&nbsp;Jaak Kals ,&nbsp;Jaan Eha ,&nbsp;Kaspar Tootsi ,&nbsp;Aigar Ottas ,&nbsp;Anneli Piir ,&nbsp;Mihkel Zilmer","doi":"10.1016/j.ijcme.2016.05.001","DOIUrl":"10.1016/j.ijcme.2016.05.001","url":null,"abstract":"<div><h3>Background/objectives</h3><p>Metabolomic profiling allows to take a detailed look at lipid metabolism and to study the levels and roles of its numerous intermediates and products in the pathogenesis of cardiovascular disease (CVD). This study aimed to investigate the relationship between the metabolic profiles of lipid metabolism, arterial stiffness and hemodynamics in patients with coronary artery disease (CAD) and for healthy controls.</p></div><div><h3>Methods</h3><p>Serum levels of 186 metabolites were determined with the AbsoluteIDQ™ p180 kit (BIOCRATES Life Sciences AG, Innsbruck, Austria). The technique of applanation tonometry was used for non-invasive pulse wave analysis and carotid-femoral pulse wave velocity (cf-PWV) assessments. Principal component analysis (PCA) was carried out in order to reduce the large number of correlated metabolites to fewer uncorrelated factors.</p></div><div><h3>Results</h3><p>Elevated levels of C16:1, C18:1, C3-DC(C4-OH), PC aa C40:6, Met-SO/Met, and reduced levels of lysoPC a C18:2 were observed in the CAD group compared to the healthy controls. The cf-PWV showed positive correlations with C14, C16:1, (C2<!--> <!-->+<!--> <!-->C3)<!--> <!-->/<!--> <!-->C0, C2<!--> <!-->/<!--> <!-->C0 and the CPT-1 ratio for the CAD group. Moreover, PCA-derived factor 3 (medium- and long-chain acylcarnitines) proved to be an independent determinant of cf-PWV for these patients.</p></div><div><h3>Conclusions</h3><p>We demonstrated an independent association between the serum medium- and long-chain acylcarnitine profile and aortic stiffness for the CAD patients. In addition to the lipid-related classical CVD risk markers, the intermediates of lipid metabolism may serve as novel indicators for altered vascular function.</p></div>","PeriodicalId":73333,"journal":{"name":"IJC metabolic & endocrine","volume":"11 ","pages":"Pages 13-18"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcme.2016.05.001","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72458651","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deteriorated clinical outcome in coronary artery disease patients with a high prevalence of Porphyromonas gingivalis infection","authors":"Daisuke Tezuka ,&nbsp;Jun-ichi Suzuki ,&nbsp;Hisanori Kosuge ,&nbsp;Norio Aoyama ,&nbsp;Yuichi Izumi ,&nbsp;Shunji Yoshikawa ,&nbsp;Yasuhiro Maejima ,&nbsp;Takashi Ashikaga ,&nbsp;Kenzo Hirao ,&nbsp;Mitsuaki Isobe","doi":"10.1016/j.ijcme.2016.05.006","DOIUrl":"10.1016/j.ijcme.2016.05.006","url":null,"abstract":"<div><h3>Background</h3><p><em>Porphyromonas gingivalis</em> (<em>P. gingivalis</em>) is known as the main pathogen to progress periodontitis. The aim of this study was to clarify oral infection <em>P. gingivalis</em> in patients with coronary computed tomography angiography (CTA) diagnoses.</p></div><div><h3>Methods</h3><p>The subjects were 50 consecutive patients who were examined by coronary CTA. Their saliva was examined for the presence of <em>P. gingivalis</em> by 16S rRNA-based polymerase chain reaction. The subjects were divided into 3 groups: normal coronary artery (group NC), patients with coronary plaque but without history of acute coronary syndrome (ACS) (group CP), and post-ACS (group pACS) according to their findings on CT and medical history. We assessed coronary risk factors and C-reactive protein (CRP) levels. Their medical records for the last 5<!--> <!-->years were analyzed.</p></div><div><h3>Results</h3><p><em>P. gingivalis</em> infection was observed in 37.5% NC (N<!--> <!-->=<!--> <!-->16), 65.2% of the CP (N<!--> <!-->=<!--> <!-->23), and 90.9% of the pACS patients (N<!--> <!-->=<!--> <!-->11). CRP levels in patients with <em>P. gingivalis</em> infection were higher than in those without the infection (0.22<!--> <!-->±<!--> <!-->0.30 vs 0.07<!--> <!-->±<!--> <!-->0.10<!--> <!-->mg/dL; p<!--> <!-->=<!--> <!-->0.031). Multiple regression analysis revealed that <em>P. gingivalis</em> (p<!--> <!-->=<!--> <!-->0.047) was statistically correlated with coronary artery disease (CAD), as well as other coronary risk factors. The patients with a <em>P. gingivalis</em> infection exhibited lower event free survival ratio (p<!--> <!-->=<!--> <!-->0.0348).</p></div><div><h3>Conclusions</h3><p><em>P. gingivalis</em> was identified by sensitive detection in patients with CAD, diagnosed by coronary CTA. <em>P. gingivalis</em> in oral saliva can be a potential marker which is associated with clinical outcomes in patients with CAD.</p></div>","PeriodicalId":73333,"journal":{"name":"IJC metabolic & endocrine","volume":"11 ","pages":"Pages 7-12"},"PeriodicalIF":0.0,"publicationDate":"2016-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcme.2016.05.006","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74212959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"N-terminal-pro-brain natriuretic peptide elevations in the course of septic and non-septic shock reflect systolic left ventricular dysfunction assessed by transpulmonary thermodilution","authors":"A.B. Johan Groeneveld ,&nbsp;Ronald J. Trof","doi":"10.1016/j.ijcme.2016.01.002","DOIUrl":"10.1016/j.ijcme.2016.01.002","url":null,"abstract":"<div><h3>Background</h3><p>The cardiac correlates, if any, of N-terminal probrain natriuretic peptide (NT-proBNP) levels in septic and non-septic shock patients remain controversial.</p></div><div><h3>Methods</h3><p>In the 38 septic and 22 non-septic shock patients in the transpulmonary thermodilution arm of a previous 2-center randomized controlled trial comparing pulmonary artery catheterization with transpulmonary thermodilution, serial (daily for 3<!--> <!-->days) and paired measurements (n<!--> <!-->=<!--> <!-->145) were obtained of NT-proBNP and transpulmonary dilution variables as global ejection fraction (GEF), left ventricular preload-recruitable stroke work (PRSW) and diastolic compliance.</p></div><div><h3>Results</h3><p>Elevated NT-proBNP inversely related to low GEF and PRSW in pooled data (r<!--> <!-->=<!--> <!-->−<!--> <!-->0.45, P<!--> <!-->&lt;<!--> <!-->0.001). The 72<!--> <!-->h course of NT-proBNP was inversely associated with PRSW, independent of age, gender, creatinine, norepinephrine treatment and diastolic compliance, without differences between septic and non-septic shock. Over the 72<!--> <!-->h study period, NT-proBNP levels were higher in 28<!--> <!-->day non-survivors than survivors, independent of type of shock and disease severity.</p></div><div><h3>Conclusions</h3><p>In septic and non-septic shock, NT-proBNP elevations reflect systolic left ventricular dysfunction and are associated with a poor outcome. They may help recognition of cardiac dysfunction in shock and its management when invasive hemodynamic monitoring is not yet instituted.</p></div>","PeriodicalId":73333,"journal":{"name":"IJC metabolic & endocrine","volume":"10 ","pages":"Pages 30-35"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcme.2016.01.002","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"75479029","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The autocrine role of tryptase in pressure overload-induced mast cell activation, chymase release and cardiac fibrosis","authors":"Jianping Li ,&nbsp;Shaiban Jubair ,&nbsp;Scott P. Levick ,&nbsp;Joseph S. Janicki","doi":"10.1016/j.ijcme.2015.11.003","DOIUrl":"10.1016/j.ijcme.2015.11.003","url":null,"abstract":"<div><h3>Background</h3><p>Cardiac mast cell (MC) proteases, chymase and tryptase, increase proliferation and collagen synthesis in cultured cardiac fibroblasts. However, the question as to why preventing individually the actions of either protease prevents fibrosis when both are released upon MC activation remains unanswered. Since tryptase has the ability to activate MCs in noncardiac tissues via the protease-activated receptor-2 (PAR-2), there is the possibility that its, in vivo, fibrotic role is due to its ability to induce MC degranulation thereby amplifying the release of chymase.</p></div><div><h3>Methods</h3><p>This study sought to delineate the interactions between tryptase and chymase in myocardial remodeling secondary to transverse aortic constriction (TAC) for 5<!--> <!-->weeks in male Sprague–Dawley rats untreated or treated with either the tryptase inhibitor, nafamostat mesilate or MC membrane stabilizing drug, nedocromil (n<!--> <!-->=<!--> <!-->6/group). In addition, ventricular slices from 6 rat hearts were incubated with tryptase, tryptase plus nafamostat mesilate or the chymase inhibitor chymostatin for 24<!--> <!-->h.</p></div><div><h3>Results and conclusion</h3><p>The results indicate the presence of PAR-2 on MCs and that tryptase inhibition and nedocromil prevented TAC-induced fibrosis and increases in MC density, activation, and chymase release. Tryptase also significantly increased chymase concentration in ventricular slice culture media, which was prevented by the tryptase inhibitor. Hydroxyproline concentration in culture media was significantly increased with tryptase incubation as compared to the control group and the tryptase group incubated with nafamostat mesilate or chymostatin. We conclude that tryptase contributes to TAC-induced cardiac fibrosis primarily via activation of MCs and the amplified release of chymase.</p></div>","PeriodicalId":73333,"journal":{"name":"IJC metabolic & endocrine","volume":"10 ","pages":"Pages 16-23"},"PeriodicalIF":0.0,"publicationDate":"2016-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.ijcme.2015.11.003","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"72857526","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}