Acta Facultatis Pharmaceuticae Universitatis Comenianae最新文献

{"title":"Clinical significance of anti-C1q antibodies in SLE","authors":"I. Stiborová, V. Král, J. Rovenský, S. Blažíčková","doi":"10.1515/afpuc-2015-0045","DOIUrl":"https://doi.org/10.1515/afpuc-2015-0045","url":null,"abstract":"Abstract Lupus nephritis (LN) is a severe and frequent complication of systemic lupus erythematosus (SLE). Untreated cases very often lead to patients’ death; therefore, it is important to use markers sensitive and specific enough for the diagnosis and subsequent monitoring of nephritis. Autoantibodies against double-stranded DNA (anti-dsDNA) are believed to play a major role in SLE in general and so their significance in prediction and monitoring of glomerular inflammation is low. For prediction of renal flares and effective, well-timed therapy, it is required to have an appropriate marker available. In our study, we have tested sera of 85 SLE patients with or without LN. The criterion for LN determination was the degree of proteinuria (persistent proteinuria > 0.5 g/day, according to ACR criteria for LN). Disease activity was described by SLE disease index (SLEDAI) score, renal functions were stated according to British Isles Lupus Assessment Group score. There were anti-C1q, total anti-dsDNA and high-avidity anti-dsDNA detected in the patients’ sera. We did not find any significant difference in average SLEDAI value between patients with renal and non-renal organ complications. Positivity of anti-C1q was more frequent in patients with nephritis than in those without any history of renal disease (58.3 vs. 39.1%). Higher prevalence of these antibodies was evident in patients with clinically active LN than in those without renal improvement (73.1 vs. 39.1%). When comparing anti-C1q with antibodies against structures of DNA, significant differences were found in case of high avidity anti-dsDNA. Our results have confirmed the studies showing that anti-C1q antibodies could serve as a reliable serological marker of LN activity along with other laboratory tests. Detection of anti-C1q together with high avidity anti-dsDNA antibodies seems to be a good algorithm for the prediction of possible renal flares in SLE patients.","PeriodicalId":7321,"journal":{"name":"Acta Facultatis Pharmaceuticae Universitatis Comenianae","volume":"11 1","pages":"15 - 20"},"PeriodicalIF":0.0,"publicationDate":"2015-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79130108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Consumer satisfaction with pharmaceutical care in Slovak community pharmacies / Spokojnosť klientov lekární s lekárenskou starostlivosťou na Slovensku","authors":"D. Mináriková, I. Malovecká, V. Foltán","doi":"10.2478/afpuc-2014-0015","DOIUrl":"https://doi.org/10.2478/afpuc-2014-0015","url":null,"abstract":"Abstract The aim of this pilot study was to evaluate consumer satisfaction with pharmaceutical care provided in community pharmacies at the region level. Expectations and opinions of consumers were also confronted with the assumptions of pharmacists. Up to 82.0% of consumers confirmed their satisfaction with the care given to them by the pharmacist. A key factor of consumer satisfaction has been the professional (78.7%) and human approach (83.5%) of the pharmacist. A total of 73.6% of consumers in the survey accepted the pharmacist as an expert in medicines. The pharmacist´s self-evaluation was more negative. Monitoring and evaluation of consumer satisfactory with pharmaceutical care is not only essential for improvement of the quality of the healthcare system and for the implementation of pharmaceutical care focussed on the patient, but it may influence the economic and financial outcomes of entities providing pharmaceutical care in community pharmacies. Slovak abstract Cieľom tejto pilotnej štúdie bolo zhodnotiť spokojnosť zákazníkov s lekárenskou starostlivosťou poskytovanou vo verejných lekárňach na lokálnej úrovni. Očakávania a názory zákazníkov boli zároveň porovnané s predpokladmi lekárnikov. Až 82,0% zákazníkov potvrdilo svoju spokojnosť so starostlivosťou, ktorú im poskytuje lekárnik. Ako kľúčový faktor spokojnosti zákazníkov sa ukázal profesionálny (78,7%) a ľudský prístup (83,5%) lekárnika. 73,6% zákazníkov v našom prieskume akceptovalo lekárnika ako odborníka na lieky. Sebahodnotenie lekárnikov bolo negatívnejšie. Monitoring a hodnotenie spokojnosti zákazníkov s lekárenskou starostlivosťou je nevyhnutné nielen pre zlepšenie kvality systému zdravotnej starostlivosti a pre implementáciu lekárenskej starostlivosti orientovanej na pacienta, ale môže ovplyvniť ekonomické a finančné výsledky subjektov poskytujúcich lekárenskú starostlivosť vo verejných lekárňach.","PeriodicalId":7321,"journal":{"name":"Acta Facultatis Pharmaceuticae Universitatis Comenianae","volume":"20 1","pages":"25 - 30"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"87814233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The exploitation of micromorphological parameters for identification in the section / Mentha Využitie mikromorfologických parametrov pri identifikácii v sekcii Mentha","authors":"S. Fialová, L. Halušová, D. Tekeľová, D. Grančai","doi":"10.1515/afpuc-2015-0004","DOIUrl":"https://doi.org/10.1515/afpuc-2015-0004","url":null,"abstract":"Abstract The identification of species in the genus Mentha is especially difficult because of the ease of hybridization, favoured by gynodioecy, which is further complicated by polymorphism, cultivation, polyploidy and vegetative propagation. This all explains the genicpopulation and microevolutionary background for variability of mints. In this work we studied the usage of micromorphological and phytochemical parameters in identification and characterization of selected Mentha species. On abaxial surface of leaves of 20 Mentha populations we determined the size and the number of peltate glandular trichomes. The essential oil was analysed by GC MS. We identified and characterized populations of M. × piperita, M. spicata, M. spicata subsp. condensata, M. spicata var. crispa, M. spicata var. citrata, M. × gentilis, M. aquatic, M. arvensis amd M. longifolia. The size and the number of peltate glandular trichomes contribute to characterisation of some Mentha species (especially in M. longifolia and M. × piperita). Slovak Abstract Identifikacia druhov v rode Mentha L. je obzvlašť naročna. Je to sposobene ľahkym križenim podporenym gynodioeciou, ktora je ďalej skomplikovana polymorfizmom, pestovanim, polyploiditou a vegetativnym rozmnožovanim, čo vysvetľuje genetickopopulačne a mikroevolučne pozadie premenlivosti v rode Mentha. V tejto praci sme študovali využitie mikromorfologickych a fytochemickych parametrov pri identifikacii a charakterizacii vybranych druhov rodu Mentha. Na spodnej pokožke listov 20 populacii Mentha sme stanovili veľkosť a počet „žliazok typu Lamiaceae“. Silicu sme analyzovali pomocou GC MS. Identifikovali a charakterizovali sme populacie druhov M. × piperita, M. spicata, M. spicata subsp. condensata, M. spicata var. crispa, M. spicata var. citrata, M. × gentilis, M. aquatic, M. arvensis a M. longifolia. Sledovanie veľkosti a počtu „žliazok typu Lamiaceae prispievaju k charakterizacii niektorych druhov Mentha (najma M. longifolia a M. × piperita).","PeriodicalId":7321,"journal":{"name":"Acta Facultatis Pharmaceuticae Universitatis Comenianae","volume":"366 1","pages":"14 - 9"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83567436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hydroxycinnamic derivatives content in plant organs linked to harvest time of Salvia officinalis L. cv. ‘Krajová’ / Obsah hydroxyškoricových derivátov v rastlinných orgánoch Salvia officinalis L. cv. ‘Krajová’ v závislosti od termínu zberu","authors":"D. Tekeľová, J. Tóth, S. Czigle, A. Koutsoulas","doi":"10.1515/afpuc-2015-0005","DOIUrl":"https://doi.org/10.1515/afpuc-2015-0005","url":null,"abstract":"Abstract Salvia officinalis L. (sage) is an important essential oil containing Mediterranean medicinal plant that is commonly cultivated for pharmaceutical uses. In addition to essential oil, other compounds participate on the biological effects of sage, mainly diterpenes, triterpenes and phenolic compounds of the hydroxycinnamic and flavonoid types. Sage essential oil content is known to differ in plant parts, and to be influenced by plant phenophase, climatic and edaphic conditions. This work deals with the study of total hydroxycinnamic derivatives (THD), and specifically rosmarinic acid (RA), content variation in different aerial parts of sage linked to different harvest times. Dry sage leaves (Salviae officinalis folium) THD content was quantified using a pharmacopoeial method, and varied between 3.06 % and 3.52 % in different harvest times, with maxima in youngest plant shoot leaves and newly-grown shoots in September. Sage stems showed similar THD content variations when linked to harvest times, however with lower percentage, 1.33 - 3.04 %. Rosmarinic acid variability showed the same trends, its content ranged between 0.76 % and 1.65 % in leaves, and between 0.19 % and 0.83 % in stems, respectively. Highest percentages of both THD and RA were found in top leaves, lowest in leaves from the middle of the stem. When linked to plant phenophase, the content of THD and RA decreased at flowering time. Slovak abstract Salvia officinalis L. (šalvia lekarska) je vyznamnou silicovou liečivou rastlinou domacou v oblasti Stredomoria, pre farmaceuticke učely sa pestuje. Okrem silice sa na biologickom učinku rastliny podieľaju hlavne diterpeny, triterpeny a fenolove latky typu hydroxyškoricovych derivatov a flavonoidov. Je zname, že obsah a kvalita silice v šalvii koliše v zavislosti od rastlinnej časti, vyvinovej fazy, klimatickych a podnych podmienok. V našej praci sme sledovali kolisanie obsahu celkovych hydroxyškoricovych derivatov (THD) a samotnej kyseliny rozmarinovej (RA) v nadzemnych častiach šalvie lekarskej v roznych terminoch zberu. Obsah THD v suchych listoch (Salviae officinalis folium) stanoveny liekopisnou metodou kolisal v jednotlivych terminoch zberu od 3,06 % do 3,52 %, najvyšši bol v listoch z vyhonkov najmladšich rastlin a z novonarastenych vyhonkov v septembri. Podobne kolisanie obsahu THD v jednotlivych zberoch bolo aj v stonkach, tie však obsahovali len 1,33 - 3,04 %. Rovnaku variabilitu obsahu sme zaznamenali pri kyseline rozmarinovej, jej obsah v listoch kolisal od 0,76 % do 1,65 % a v stonkach od 0,19 % do 0,83 %. Najvyšši obsah THD a RA bol vo vrcholovych listoch, najnižši v listoch umiestnenych v strede stonky. Počas kvitnutia rastliny sa obsah THD a RA v listoch znižil.","PeriodicalId":7321,"journal":{"name":"Acta Facultatis Pharmaceuticae Universitatis Comenianae","volume":"17 1","pages":"40 - 46"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"73617749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elicitation – a tool to improve secondary metabolites production in Melissa Officinalis L. Suspension cultures / Elicitácia ako nástroj na zlepšenie produkcie sekundárnych metabolitov v suspenzných kultúrach Melissa Officinalis L.","authors":"J. Urdová, M. Rexová, P. Mučaji, A. Balažová","doi":"10.1515/afpuc-2015-0012","DOIUrl":"https://doi.org/10.1515/afpuc-2015-0012","url":null,"abstract":"Abstract Our recent study is focussed on the investigation of the influence of biotic (Botrytis cinerea, methyl jasmonate) and abiotic (cadmium and cobalt chloride) elicitors on Melissa officinalis L. (lemon balm) suspension cultures production properties. The plant material was treated with different concentrations of methyl jasmonate (10 and 100 μmol.l−1), with a phytopathogenic fungus hydrolyzate (Botrytis cinerea, 1 and 2 ml with glucose equivalent 24 μg.ml−1), cadmium and cobalt (II) chloride (both at concentrations 100 μmol.l−1 and 1 μmol.l−1). Elicited suspension cultures were harvested after 24, 48 and 72 h of elicitor treatment. The elicitation effect was evaluated based on hydroxycinnamic acid derivatives content expressed as rosmarinic acid in dose- and time-dependent manners. The Botrytis cinerea hydrolyzate exhibited the best effect on Melissa officinalis L. suspension cultures production properties among biotic elicitors (glucose equivalent 24 mg.ml−1, 1 ml, after 72 h of treatment). The hydroxycinnamic acid derivatives content increased in triplicate compared to non-elicited samples. Methyl jasmonate affected the suspension cultures production properties moderately at concentration 10 μmol.l−1. Chlorides salts of cobalt and cadmium stimulated phenolic compounds production effectively at concentrations 100 μmol.l−1. The presence of cobalt and cadmium ions in suspension cultures growth media increased the hydroxycinnamic acid derivatives content twofold and quadruple, respectively. Slovak abstract Súčasná práca je zameraná na štúdium vplyvu biotických (Botrytis cinerea, metyljazmonát) a abiotických elicitorov (chlorid kademnatý a chlorid kobaltnatý) na produkčné vlastnosti suspenzných kultúr medovky lekárskej (Melissa officinalis L.). Rastlinný materiál bol elicitovaný metyljazmonátom (10 and 100 μmol.l-1), hydrolyzátom bunkovej steny fytopatogénnej huby Botrytis cinerea (1 a 2 ml s glukózovým ekvivalentom 24μg.ml-1), chloridom kademnatým a kobaltnatým (oba v koncentráciách 100 and 1 μmol.l-1). Elicitované kultúry boli vystavené expozícii elicitorov po dobu 24, 48 a 72 h. Efekt elicitácie sa hodnotil na základe obsahu derivátov kyseliny hydroxyškoricovej, vyjadrených ako kyseliny rozmarínová, v závislosti od koncentrácie použitého elicitora a dĺžky jeho pôsobenia. Spomedzi testovaných biotických elicitorov najúčinnejšie podporil produkčné vlastnosti suspenzných kultúr Melissa officinalis L. hydrolyzát z Botrytis cinerea (glukózový ekvivalent 24 mg.ml-1, 1 ml, doba pôsobenia 72 h). Obsah derivátov kyseliny hydroxyškoricovej sa po elicitácii zvýšil trojnásobne v porovnaní s neelicitovanými kultúrami. Produkčná schopnosť suspenzných kultúr elicitovaných metyljazmonátom sa mierne zvýšila len pri použití koncentrácie 10 μmol.l-1. Chlorid kobaltnatý a kademnatý efektívne stimulovali produkciu fenolových látok pri koncentrácii 100 μmol.l-1. Prítomnosť iónov kobaltu a kadmia v rastových médiách suspenzných kultúr viedla k dvoj-, resp. štvorn","PeriodicalId":7321,"journal":{"name":"Acta Facultatis Pharmaceuticae Universitatis Comenianae","volume":"18 1","pages":"46 - 50"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"79924387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Polyphenolic compounds and essential oil analysis of selected species of the genus Thymus / Analýza fenolových zložiek a analýza silice vybraných druhov rodu Thymus","authors":"M. Kameníková, S. Fialová, A. Ťažký, I. Čičová","doi":"10.1515/afpuc-2015-0009","DOIUrl":"https://doi.org/10.1515/afpuc-2015-0009","url":null,"abstract":"Abstract The content of phenolic compounds (total phenolic compounds, tannins, flavonoids and anthocyanidins) of three species of thyme (Thymus pulegioides, Thymus pannonicus, Thymus praecox) of different origin (Bohemian-Moravian highlands, Křivoklat and Považsky Inovec) was determined using spectrophotometric methods of European Pharmacopoeia 8th edition. Furthermore, the determination of the content of essential oil and analysis of its constituents was realised. The amount of total phenolics was determined by a spectrophotometric method using the Folin-Ciocalteu reagent. Their content ranged from 3.87 to 8.86%. The content of tannins was established on a preliminary determination of the total phenolics, followed by adsorption of tannins on hide powder. The amount of tannins in our samples varied from 1.96 to 5.65%. The content of total flavonoids was determined by a spectrophotometric method using aluminium chloride. Quantitative analysis of flavonoids has shown that content in our samples ranged from 0.59 to 1.52% expressed as luteolin-7-O-glucoside (λ = 392 nm) and from 0.41 to 1.12% expressed as rutin (λ = 420 nm). Anthocyanins represent a small amount of total polyphenol content in Thymus species. In our samples, the content of anthocyanins expressed as cyanidin-3-O-glucoside varied from 0.02 to 0.1%. The determination of essential oil was realised by method of European Pharmacopoeia 8th edition. Its contents in our samples ranged from 0.2 to 0.75%. Gas chromatography-mass spectrometry was used for essential oil analysis. According to the presence of main monoterpene in Thymus essential oil, we can distinguish three chemotypes: thymol, carvacrol and linalool. Due to variations of chemical constituents of thyme essential oil, it was possible to observe a relationship between occurrence of certain chemotype and origin of sample. Differences in content of flavonoids have not shown a significant relationship to locality of origin. Slovak abstract Predložena praca bola zamerana na stanovenie obsahu vybranych fenolovych zlučenin (celkove fenoly, triesloviny, flavonoidy a antokyany) troch roznych druhov rodu Thymus (T. pulegioides, T. pannonicus, T. praecox) s rozdielnym povodom (Českomoravska vrchovina, Křivoklat a Považsky Inovec). Na stanovenie tychto zložiek boli využite spektrofotometricke metody Europskeho liekopisu, 8. vydanie. Zaroveň bol stanoveny aj obsah silice a bola vykonana analyza jej zložiek. Obsah celkovych polyfenolov bol stanoveny spektrofotometrickou metodou s využitim Folin-Ciocalteovho činidla. Obsah kolisal od 3.87 % do 8,86 %. Obsah trieslovin bol realizovany na zaklade predošleho stanovenia obsahu celkovych fenolov, a to adsorpciou trieslovin na kožny prašok. Ich obsah sa pohyboval v rozmedzi 1,96 % do 5,65 %. Na stanovenie obsahu flavonoidov bola využita spektrofotometricka metoda s chloridom hlinitym. Obsah flavonoidov vyjadrenych ako luteolin-7-O-glukozid (λ = 392 nm) kolisal od 0,59 % do 1,52 % a obsah flavonoidov vyj","PeriodicalId":7321,"journal":{"name":"Acta Facultatis Pharmaceuticae Universitatis Comenianae","volume":"76 1","pages":"12 - 17"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"74421777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Prosperity of community pharmacy evaluated by gross and net profit and suggested corrective measures. 10 years study / Prosperita verejnej lekárne hodnotená prostredníctvom hrubého a čistého zisku a opatrenia vedúce k ich náprave. 10 ročná štúdia","authors":"I. Malovecká, K. Papargyris, D. Minarikova, V. Foltán, A. Jankovská","doi":"10.1515/afpuc-2015-0015","DOIUrl":"https://doi.org/10.1515/afpuc-2015-0015","url":null,"abstract":"Abstract Monitoring, calculation and assessment of community pharmacy prosperity with the help of financial analysis indicators for years 2003-2012, using financial statements was conducted, with respect to profitability parameters such as gross and net profit ratios. These ratios reflect various changes that hold between years 2003 and 2012. Under the time of financial crisis, recession and serious socioeconomic changes the profitability parameter gross profit ranged from x2003-2011=14.3-22.8% (average=19.2%, mean=19.8%, σ=2.4), but in 2012 decreased on 14.3%. Net profit ranged x2003-2011 = 2.3-18.3% (average=14.6%, mean =16.6%, σ = 4.9), while in 2012 reached only 2.3%. All changes that have taken place in the society had impact on community pharmacy finance by worsening its profitability. Therefore, the stability of community pharmacy may be threatened and may affect its future performance.","PeriodicalId":7321,"journal":{"name":"Acta Facultatis Pharmaceuticae Universitatis Comenianae","volume":"24 1","pages":"20 - 24"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"83386063","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Medication review in inpatients at cardiology clinic / Prehodnotenie terapie u hospitalizovaných pacientov na kardiologickej klinike","authors":"B. Kováčová, J. Hasilla, A. Ďurišová","doi":"10.1515/afpuc-2015-0002","DOIUrl":"https://doi.org/10.1515/afpuc-2015-0002","url":null,"abstract":"Abstract This study deals with effect of hospital pharmacist on solving drug-related problems (DRP) in inpatients. The study was carried out as a prospective 5-week study at the Cardiology Clinic, Teaching Hospital, Nitra. The study group included 73 inpatients. Pharmacotherapy of each patient was analysed for DRP within 24-48 h after admission. Information on patients was collected from electronic database, medical reports, communication with attending doctors and ward rounds. Patients’ age, medical history, diagnoses, medication prescribed during hospitalisation and laboratory test results were assessed for further pharmacotherapy rationalisation. Pharmaceutical Care Network Europe (PCNE) classification scheme for DRP V6.2 was used to characterise DRP. There were 36 DRP identified in 37% inpatients (n = 27). The most frequent causes of DRP were dose-related (n = 13; 26%) and use Slovak abstract Táto práca poukazuje na možnosti farmaceuta pri identifikovaní a riešení farmakoterapeutických problémov (DRP) u hospitalizovaných pacientov. Sledovaný súbor tvorilo 73 pacientov, ktorí boli hospitalizovaní na Kardiologickej klinike. U každého pacienta zaradeného do štúdie sa prehodnotila terapia do 24-48 hodín od prijatia k hospitalizácii. Informácie o pacientoch sme čerpali z elektronickej databázy, chorobopisov, osobnej konzultácie s lekárom a lekárskych vizít. Sledovanými údajmi pri racionalizácii terapie bol vek pacienta, lieková anamnéza, diagnózy, ordinovaná terapia a laboratórne parametre. Identifikáciu a riešenie DRP sme vykonávali podľa PCNE klasifikácie V6.2. V 37% (n=27) chorobopisov sme identifikovali 36 DRP. Najčastejšou príčinou DRP bola veľkosť dávky (n=13; 26%) a výber nevhodného alebo kontraindikovaného liečiva (n=11; 22%). Cieľom tejto","PeriodicalId":7321,"journal":{"name":"Acta Facultatis Pharmaceuticae Universitatis Comenianae","volume":"20 1","pages":"15 - 19"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"86030249","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phenolic compounds variation in Mentha L. Species in the course of a four-years period / Kolísanie fenolových látok v rôznych druhoch Mentha L. Počas 4 ročného obdobia","authors":"S. Fialová, D. Tekeľová, K. Rendeková, J. Klinčok, M. Kolařík, K. Kurucová, D. Grančai","doi":"10.1515/afpuc-2015-0013","DOIUrl":"https://doi.org/10.1515/afpuc-2015-0013","url":null,"abstract":"Abstract Mints rank among the most important Lamiaceae plants. In addition to essential oil, they contain many valuable phenolic compounds, including flavonoids and phenolic acids that participate in mints’ pharmacological properties. In this work, we examined the contents of phenolic compounds variation in the course of 4 years of vegetation. We compared the contents of total hydroxycinnamic derivatives expressed as rosmarinic acid (λ = 505 nm), total polyphenols and tannins expressed as rosmarinic acid (λ = 760 nm), and flavonoids expressed as luteolin-7-O-glucoside (λ = 392 nm) and quercetin (λ = 420 nm) in 1-, 2-, 3-, and 4-year-old plants’ dry leaves, respectively. Spectrophotometric methods of the European Pharmacopoeia were employed. Our results show high levels of active phenolic compounds, particularly in 3- and 4-year-old plants. Slovak abstract Mäty patria medzi významné rastliny čeľade Lamiaceae. Okrem silice obsahujú množstvo cenných fenolových látok, vrátane flavonoidov a fenolových kyselín, ktoré sa spolupodieľajú na farmakologickej aktivite mäty. V našej práci sme hodnotili kolísanie obsahu fenolových látok počas 4 rokov vegetácie. Pri 1- až 4-ročných rastlinách sme porovnali v suchých listoch obsah hydroxyškoricových derivátov vyjadrených ako kyselina rozmarínová (λ = 505 nm), polyfenolov a trieslovín vyjadrených ako kyselina rozmarínová (λ = 760 nm) a flavonoidov vyjadrených ako luteolín-7-O-glukozid (λ = 392 nm) a kvercetín (λ = 420 nm). Využili sme spektrofotometrické metódy Európskeho liekopisu. Naše výsledky poukazujú na vysoké hodnoty fenolových látok najmä v 3- a 4-ročných porastoch.","PeriodicalId":7321,"journal":{"name":"Acta Facultatis Pharmaceuticae Universitatis Comenianae","volume":"7 1","pages":"2 - 7"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"78645108","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Factors affecting the actual yield of active substances from anthocyanin-rich berries / Faktory ovlivňující skutečný výtěžek aktivních látek z plodů bohatých na anthokyany","authors":"A. Polášková, J. Spilková, A. Matějíček, H. Studená","doi":"10.1515/afpuc-2015-0006","DOIUrl":"https://doi.org/10.1515/afpuc-2015-0006","url":null,"abstract":"Abstract The content of anthocyanins in fruits (Sambucus nigra. Vaccinium myrtillus. Aronia melanocarpa) and in some fruit (berry) products was determined by differential spectrophotometry, a method recommended by AOAC International (former Association of Official Analytical Chemists). The method takes advantage of the reversible pH-dependent transformation of the colour to the colourless form of anthocyanins. The procedure and evaluation were refined to allow measurements of incompletely specified anthocyanin mixtures. Parameters of the determination (buffer composition, pH, stabilisation of samples, reference standards) were optimised. Using the modified method, anthocyanins in various matrices (fruits, syrups, wines) were measured with the aim to explain possible losses of these active substances during processing and/or storage of fruit products. Real samples of commercial products recommended conventionally as a valuable source of antioxidants were analysed and in some of them, only low levels of anthocyanins were found. In a model arrangement, anthocyanins in the skin and the pulp of Sambucus nigra fruits were extracted separately. In all samples of various cultivars, anthocyanins were present predominantly in the skin (up to 90% of the total content). Considering this, among other factors, a suitable extraction technology might improve the quality of berry products substantially. Slovak abstract Obsah anthokyanů v plodech (Sambucus nigra. Vaccinium myrtillus. Aronia melanocarpa) a v některych produktech z plodů byl stanoven diferenčni spektrofotometrii, metodou doporučenou AOAC International (dřivějši Associace oficialnich analytickych chemiků). Metoda využiva reverzibilni transformace barevne formy anthokyanů na bezbarvou v zavislosti na pH. Postup i způsob hodnoceni byly upraveny tak, aby umožnily měřeni i u ne zcela přesně definovanych směsi anthokyanů. Byly optimalizovany podminky stanoveni (složeni pufrů, pH, stabilizace vzorků, referenčni standardy). Pomoci modifikovane metody byly změřeny anthokyany v různych matricich (plody, sirupy, vino) s cilem vysvětlit možne ztraty aktivnich latek při zpracovani plodů a/nebo uchovavani produktů. Byly analyzovany vzorky vyrobků dostupnych v obchodni siti a doporučovanych obvykle jako cenny zdroj antioxidantů - v některych byl nalezen jen maly obsah anthokyanů. V modelovem uspořadani byly odděleně extrahovany anthokyany ze slupek a dužniny plodů Sambucus nigra. Ve všech vzorcich z různych kultivarů byly anthokyany přitomny převažně ve slupkach (až 90 % celkoveho obsahu). Tyto i dalši faktory je nutno brat v uvahu při vyvoji vhodne technologie, ktera by mohla podstatně zlepšit kvalitu vyrobků z plodů.","PeriodicalId":7321,"journal":{"name":"Acta Facultatis Pharmaceuticae Universitatis Comenianae","volume":"11 1","pages":"31 - 40"},"PeriodicalIF":0.0,"publicationDate":"2015-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"85023777","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}