Fungal systematics and evolution最新文献

{"title":"Introducing two new genera, <i>Paivomyces gen. nov.</i> and <i>Pseudoselenophoma gen. nov.</i>, in <i>Saccotheciaceae</i> (<i>Dothideales</i>).","authors":"S S Nascimento, L O Ferro, Silva E A, Oliveira C S, Felipe M T C, Souza-Motta C M, Bezerra J D P","doi":"10.3114/fuse.2025.16.4","DOIUrl":"10.3114/fuse.2025.16.4","url":null,"abstract":"<p><p>Members of the family <i>Saccotheciaceae</i> are found in various environments and have different lifestyles. While exploring <i>Mimosa tenuiflora</i>-associated endophytic fungi in the Caatinga dry forest in Brazil, the isolates were initially identified as aureobasidium-like fungi. Based on morphological features and phylogenetic analyses using ITS, LSU, <i>tub2</i>, <i>rpb2</i>, and <i>tef1</i> gene sequences, these endophytes were placed within a distinct clade in <i>Saccotheciaceae</i>, differing from the genera currently accepted in the family. Here, we describe a new genus, <i>Paivomyces gen. nov.</i> (including <i>Paivomyces mimosae sp. nov.</i>). Additionally, during our phylogenetic analyses, we observed that <i>Selenophoma australiensis</i>, described from leaves of <i>Eucalyptus mineata</i> in Australia, could be treated as a unique lineage among the genera in the family <i>Saccotheciaceae</i>. Herein, we introduce <i>Pseudoselenophoma gen. nov.</i> (including <i>Pseudoselenophoma australiensis comb. nov.</i>) to accommodate the species. Our findings improve our understanding of the fungal diversity in the Caatinga dry forest in Brazil and clarify the phylogenetic lineages of <i>Saccotheciaceae</i>. <b>Citation:</b> Nascimento SS, Ferro LO, Silva EA, Felipe MTC, Souza-Motta CM, Bezerra JDP (2025). Introducing two new genera, <i>Paivomyces gen. nov</i>. and <i>Pseudoselenophoma gen. nov.</i>, in <i>Saccotheciaceae</i> (<i>Dothideales</i>). <i>Fungal Systematics and Evolution</i> <b>16</b>: 55-69. doi: 10.3114/fuse.2025.16.4.</p>","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"16 ","pages":"55-69"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486222/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145214665","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Hemileia ritscheliae</i>, a new species of rust fungus on <i>Aristolochia</i> sp. collected from Uganda in 1943.","authors":"J Bailey, S T Miller, L A Castlebury","doi":"10.3114/fuse.2025.16.8","DOIUrl":"10.3114/fuse.2025.16.8","url":null,"abstract":"<p><p>The genus <i>Hemileia</i> was established in 1869 and made famous by <i>H. vastatrix</i>, the agent of coffee leaf rust. This group of obligate biotrophs has grown to include more than 42 species, infecting plant hosts from 43 genera across Africa and Asia. A specimen collected in 1943 in Uganda, on the host plant <i>Aristolochia</i>, was identified to <i>Hemileia</i> sp. (BPI019726). Species identification was historically inconclusive and subsequent examinations noted that the material was insufficient. In this study, the specimen was morphologically re-examined, and partial sequences of the small subunit (18S) and large subunit (28S) of the rDNA were obtained. It was found to be sufficiently different from other species in the genus, and here described as <i>Hemileia ritscheliae sp. nov.</i>, named for the work by Anja Ritschel in compiling the <i>Hemileia</i> monograph (Ritschel 2005), and whose research greatly assisted our work. <b>Citation:</b> Bailey J, Miller ST, Castlebury LA (2025). <i>Hemileia ritscheliae</i>, a new species of rust fungus on <i>Aristolochia</i> sp. collected from Uganda in 1943. <i>Fungal Systematics and Evolution</i> <b>16</b>: 147-153. doi: 10.3114/fuse.2025.16.8.</p>","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"16 ","pages":"147-153"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486217/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145214620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diversity of fungicolous fungi associated with <i>Camarotella</i> spp. (<i>Phyllachorales</i>, <i>Sordariomycetes</i>) on <i>Cocos nucifera</i> in Brazil, including <i>Fusicolla parasitica sp. nov.</i> (<i>Nectriaceae</i>).","authors":"C E V A Sá, C M Souza-Motta, P V Tiago, A R Machado","doi":"10.3114/fuse.2025.16.9","DOIUrl":"10.3114/fuse.2025.16.9","url":null,"abstract":"<p><p><i>Camarotella torrendiella</i> and <i>Camarotella acrocomiae</i>, the causal agents of coconut black leaf spot, are of great economic importance to coconut cultivars. Species of hyperparasites have previously been reported in the stromata of these species, which delay and reduce the viability of the ascospores of these pathogens, demonstrating their bio-control potential. In order to identify and analyse the parasitic species affecting the pathogens that cause coconut black leaf spot, symptomatic leaflets exhibiting parasitized stromata of both <i>C. torrendiella</i> and <i>C. acrocomiae</i> were collected in the states of Pernambuco and Paraíba (Brazil) and the fungi present in the stromata were isolated. The hyperparasites were phylogenetically identified based on different gene regions according to genera and presented in this paper with a detailed taxonomic description as well as their abundance and constancy in the coconut grove. A new species of <i>Fusicolla</i> was described in this study as <i>Fusicolla parasitica sp. nov. Acremonium cavaraeanum</i> presented a new record for the states of Paraíba and Pernambuco. <i>Fusarium pseudocircinatum</i> and <i>Fusarium caatingaense</i> presented the first report of their fungicolous lifestyles, while <i>Purpureocillium lilacinum</i> was for the first time recorded hyperparasitizing a <i>Camarotella</i> spp. causing leaf lesions. This study shows <i>Fusicolla parasitica</i> and <i>Acremonium cavaraeanum</i> as constant and abundant species in the coconut grove, while <i>Fusarium pseudocircinatum</i> and <i>Purpureocillium lilacinum</i> are accessory and common; <i>Fusarium caatingaense</i> was considered rare and accidental. <b>Citation:</b> Sá CEVA, Souza-Motta CM, Tiago PV, Machado AR (2025). Diversity of fungicolous fungi associated with <i>Camarotella</i> spp. (<i>Phyllachorales</i>, <i>Sordariomycetes</i>) on <i>Cocos nucifera</i> in Brazil, including <i>Fusicolla parasitica sp. nov.</i> (<i>Nectriaceae</i>). <i>Fungal Systematics and Evolution</i> <b>16</b>: 155-180. doi: 10.3114/fuse.2025.16.9.</p>","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"16 ","pages":"155-180"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486220/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145214663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Expanding <i>Lycogala</i>: twenty-four new species, their morphology and phylogenetic relationships.","authors":"D Leontyev, Y Ishchenko, M Leontieva, I Yatsiuk, O Shchepin, W-L Song, S L Chen, E Moroz, M Schnittler","doi":"10.3114/fuse.2025.16.7","DOIUrl":"10.3114/fuse.2025.16.7","url":null,"abstract":"<p><p>The genus <i>Lycogala</i> was historically considered a small, morphologically uniform group of myxomycetes, with its classification remaining largely unchanged for most of the 20^th century. However, recent molecular studies have revealed exceptional cryptic diversity within the genus, challenging its traditional species delimitations. This study continues the taxonomic reassessment of <i>Lycogala</i>, integrating three-gene phylogenies and morphological analyses of 716 specimens from four continents. A total of 706 nucSSU, 273 mtSSU, and 130 <i>COI</i> sequences were analysed to delimit species within the genus using recombination test and distance-based delimitation. As a result, 716 specimens were assigned to 93 nucSSU ribogroups, which were further grouped into 63 putative morphological species. Of these, only 20 had been formally described prior to this study. Herein, we describe an additional 24 species, significantly expanding the known diversity of the genus. Phylogenetic analyses suggest the division of <i>Lycogala</i> into five major clades, which correspond to distinct morphological traits, supporting the proposal of five new sections within the genus. The study also confirms that <i>L. epidendrum</i> s. str. exhibits significant genetic polymorphism, with at least 29 subgroups detected. However, species delimitation within <i>L. epidendrum</i> remains challenging due to conflicting results from genetic markers and the presence of recombined genotypes. The study underscores the necessity of integrating molecular and morphological approaches for resolving <i>Lycogala</i> taxonomy and highlights the potential for further discoveries in myxomycete diversity. <b>Citation:</b> Leontyev D, Ishchenko Y, Leontieva M, Yatsiuk I, Shchepin O, Song W-L, Chen SL, Moroz E, Schnittler M (2025). Expanding <i>Lycogala</i>: twenty-four new species, their morphology and phylogenetic relationships. <i>Fungal Systematics and Evolution</i> <b>16</b>: 93-146. doi: 10.3114/fuse.2025.16.7.</p>","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"16 ","pages":"93-146"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486227/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145214669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Recent taxonomic discoveries suggest a host-symbiont co-migration of <i>Russula</i> subsect. <i>Castanopsidum</i> with <i>Fagaceae</i> in the Americas.","authors":"C Manz, A Ortiz-Suárez, K Adamčíková, B Looney, C R Noffsinger, M Caboň, F Hampe, M Piepenbring, T Hofmann, B Buyck, C A Parra-Aldana, S Adamčík, A Corrales","doi":"10.3114/fuse.2025.16.10","DOIUrl":"10.3114/fuse.2025.16.10","url":null,"abstract":"<p><p>Three members of ectomycorrhizal fungi belonging to <i>Russula</i> subsection <i>Castanopsidum</i> are described from neotropical montane forests dominated by species of <i>Fagaceae</i> in Panama and Colombia. <i>Russula esperanzae sp. nov</i>. from Colombia differs in the field from other members of the subsection by slender longitudinally striated red stipes as well as characteristics of spores and basidia. <i>Russula atroamethystina</i> subsp. <i>atroamethystina</i>, originally described from Costa Rica, is reported for the first time from Panama. Closely related and morphologically similar Colombian collections are described as <i>R. atroamethystina</i> subsp. <i>guacahayoensis subsp. nov</i>. and differ from the typical subspecies by longer hymenial cystidia as well as molecular sequence data. The distribution of this newly described subspecies is restricted to <i>Fagaceae</i> forests east of the Isthmus of Panama. This suggests an allopatric diversification, likely driven by the absence of <i>Fagaceae</i> in the tropical lowland forest of the Darién Gap. <i>Russula esperanzae</i> is exclusively associated with <i>Trigonobalanus excelsa</i>, a tree species of <i>Fagaceae</i> endemic to Colombia. The role of its association with this tree in the evolutionary history of the species is discussed. Based on molecular timing, species of the subsection <i>Castanopsidum</i> started to evolve approximately 15.1 M years ago (mya) and display an amphi-pacific disjunct distribution with an Asian clade that split from the American clade around 11 mya. <b>Citation:</b> Manz C, Ortiz-Suárez A, Adamčíková K, Looney B, Noffsinger CR, Caboň M, Hampe F, Piepenbring M, Hofmann T, Buyck B, Parra-Aldana CA, Adamčík S, Corrales A (2025). Recent taxonomic discoveries suggest a host-symbiont co-migration of <i>Russula</i> subsect. <i>Castanopsidum</i> with <i>Fagaceae</i> in the Americas. <i>Fungal Systematics and Evolution</i> <b>16</b>: 181-195. doi: 10.3114/fuse.2025.16.10.</p>","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"16 ","pages":"181-195"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486226/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145214638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrative taxonomy and genus delimitation in the <i>Rhizocarpaceae</i> (lichenized <i>Ascomycota</i>).","authors":"E J Möller, E Timdal, R Haugan, M Bendiksby","doi":"10.3114/fuse.2025.16.12","DOIUrl":"10.3114/fuse.2025.16.12","url":null,"abstract":"<p><p>The <i>Rhizocarpaceae</i>, a family of lichenized fungi within the <i>Ascomycota</i>, comprises approximately 160 species within five genera: <i>Catolechia</i>, <i>Epilichen</i>, <i>Haugania</i>, <i>Poeltinula</i>, and <i>Rhizocarpon</i>. <i>Rhizocarpon</i> is the most species-rich, with about 150 species predominantly inhabiting siliceous rock in boreal and arctic-alpine environments. Molecular phylogenetic studies have revealed that current taxonomy, heavily reliant on morphology, chemistry, and life strategies, renders <i>Rhizocarpon</i> paraphyletic. This study aims to elucidate the phylogenetic relationships and clarify genus delimitation within the <i>Rhizocarpaceae</i> using an integrative taxonomic approach that combines three genetic markers and a diversity of taxa covering the morphological, chemical, and ecological spectrum of the family. Our comprehensive sampling includes 50 species across the <i>Rhizocarpaceae</i> collected from diverse geographical locations and ecological settings. Our phylogenetic hypothesis is based on a concatenated dataset of two nuclear (ITS and MCM7) and one mitochondrial (mtSSU) genetic marker. Ascospore characteristics and thallus pigmentation alongside secondary metabolite profiles were mapped onto this DNA-based evolutionary framework. Our results underscore significant refinements in the classification of the <i>Rhizocarpaceae</i>, highlighting the inadequacy of traditional taxonomic markers alone to infer robust phylogenetic affiliations. We advocate for new circumscriptions of <i>Catolechia</i>, <i>Poeltinula</i>, and <i>Rhizocarpon</i> based on the molecular phylogeny and propose synonymizing <i>Epilichen</i> with <i>Catolechia</i>, the transfer of the species in the <i>R. hochstetteri</i> complex to <i>Poeltinula</i>, and the resurrection of <i>Rehmia</i>. We hence propose 24 new combinations and three typifications. Collectively, this study sets the groundwork for future research and stability in the systematics of the <i>Rhizocarpaceae</i>, augmenting our understanding of their diversity and evolutionary dynamics. <b>Citation:</b> Möller EJ, Timdal E, Haugan R, Bendiksby M (2025). Integrative taxonomy and genus delimitation in the <i>Rhizocarpaceae</i> (lichenized <i>Ascomycota</i>). <i>Fungal Systematics and Evolution</i> <b>16</b>: 215-231. doi: 10.3114/fuse.2025.16.12.</p>","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"16 ","pages":"215-231"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486223/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145214583","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring <i>Neopestalotiopsis</i> diversity associated with Blueberry leaf and twig blight in South African nurseries.","authors":"L S Van der Vyver, W De Bruin, N Siyoum, D L Nsibo, J Z Groenewald, P W Crous, L Korsten","doi":"10.3114/fuse.2025.16.3","DOIUrl":"10.3114/fuse.2025.16.3","url":null,"abstract":"<p><p>New blueberry plantings in South Africa have increased dramatically since the year 2000, exceeding the global expansion rate. The crop is, however, impacted by several economically important fungal pathogens. Lesser-known fungi such as pestalotioid species can cause leaf and twig blight, and have the potential to become a global threat to blueberry production and expansion. This study aimed to assess the presence of such fungal species in blueberry nurseries in South Africa due to the industry's exponential growth and mass introduction of new cultivars. Symptomatic leaf and twig samples were collected from six propagation nurseries, resulting in 180 pestalotioid isolates, of which a subset of 48 isolates were selected for molecular characterisation using multi-locus sequence analysis of the internal transcribed spacer region (ITS), partial translation elongation factor 1-α (<i>TEF1</i>) and partial β-tubulin (<i>TUB2</i>) gene regions. Phylogenetic analyses were performed using Maximum Likelihood, Maximum Parsimony and Bayesian Inference methods. Based on the phylogenetic analyses, all isolates clustered with three previously described species of <i>Neopestalotiopsis</i>, namely <i>N. rosae</i>, <i>N. hispanica</i> and <i>N. longiappendiculata.</i> Of these, 32 isolates were identified as <i>N. rosae</i>, followed by <i>N. hispanica</i> (12) and <i>N. longiappendiculata</i> (4). To the best of our knowledge, this is the first report of these species in South Africa. Based on phylogenetic analysis and morphological comparisons, we further recommend that <i>N. vaccinii</i> be considered a synonym of <i>N. hispanica.</i> <b>Citation:</b> Van der Vyver LS, De Bruin W, Siyoum N, Nsibo DL, Groenewald JZ, Crous PW, Korsten L (2025). Exploring <i>Neopestalotiopsis</i> diversity associated with Blueberry leaf and twig blight in South African nurseries. <i>Fungal Systematics and Evolution</i> <b>16</b>: 41-53. doi: 10.3114/fuse.2025.16.3.</p>","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"16 ","pages":"41-53"},"PeriodicalIF":0.0,"publicationDate":"2025-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12486218/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145214613","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"New treasures in <i>Cordycipitaceae</i>: Fungicolous fungi associated with <i>Pseudocercospora fijiensis</i> and <i>P. musae</i> in Brazil, including <i>Matutinistella gen. nov</i>.","authors":"F A Custódio, O L Pereira","doi":"10.3114/fuse.2025.15.06","DOIUrl":"10.3114/fuse.2025.15.06","url":null,"abstract":"<p><p><i>Cordycipitaceae</i> is a large family belonging to the order <i>Hypocreales</i>, including cosmopolitan species found on different substrates. This family includes species with different lifestyles such as entomopathogenic, endophytic, and mycoparasitic fungi. Some mycoparasitic species in this family attack phytopathogenic fungi. We identified species of <i>Cordycipitaceae</i> found during a survey of fungicolous fungi associated with <i>Pseudocercospora fijiensis</i> and <i>P. musae</i> in Brazil. Based on morphological characters combined with phylogenetic analysis of ITS, LSU, SSU<i>, RPB1, RPB2,</i> and <i>TEF1-α</i>, we propose a new genus in <i>Cordycipitaceae</i>, namely <i>Matutinistella,</i> with its type species <i>M. brasiliensis,</i> and a new species of the genus <i>Simplicillium,</i> namely <i>S. pseudocercosporicola.</i> Furthermore, we report <i>P. fijiensis</i> as a new host of the mycoparasitic fungus <i>Simplicillium lanosoniveum.</i> In this study we newly report fungicolous fungi of <i>Cordycipitaceae</i> associated with the causal agents of Black Sigatoka and Yellow Sigatoka on banana crops. We provide relevant information for future work involving control measures for these diseases that cause major losses in banana crops. <b>Citation</b>: Custódio FA, Pereira OL (2025). New treasures in <i>Cordycipitaceae</i>: Fungicolous fungi associated with <i>Pseudocercospora fijiensis</i> and <i>P. musae</i> in Brazil, including <i>Matutinistella gen. nov. Fungal Systematics and Evolution</i> <b>15</b>: 133-152. doi: 10.3114/fuse.2025.15.06.</p>","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"15 ","pages":"133-152"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11959235/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765915","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel amplified fragment length polymorphism (AFLP) markers for typing medically relevant <i>Fusarium</i> and allied fusarioid genera.","authors":"R C Monteiro, C Z Yu, S Dolatabadi, F Hagen, M Sandoval-Denis, P W Crous, M C Fisher, S S Gonçalves, Z P de Camargo, A L Hofling-Lima, A M Rodrigues","doi":"10.3114/fuse.2025.15.03","DOIUrl":"10.3114/fuse.2025.15.03","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Fusariosis is an emerging mycosis caused by diverse &lt;i&gt;Fusarium&lt;/i&gt; and allied fusarioid genera that are characterized by spindle-shaped macroconidia. These fungi possess a broad ecological distribution, causing infections in a wide diversity of hosts, spanning the animal and plant kingdoms. The spectrum of human fusariosis encompasses superficial lesions like keratitis and onychomycosis to invasive fungal diseases. Notable genera within the medically relevant fusarioid group include &lt;i&gt;Neocosmospora, Fusarium s. str.&lt;/i&gt;, and &lt;i&gt;Bisifusarium&lt;/i&gt;. While &lt;i&gt;Neocosmospora&lt;/i&gt; species (formerly &lt;i&gt;F. solani&lt;/i&gt; species complex) are primary causative agents of human fusariosis, instances involving &lt;i&gt;Fusarium s. str.&lt;/i&gt; and &lt;i&gt;Bisifusarium&lt;/i&gt; (formerly &lt;i&gt;F. dimerum&lt;/i&gt; species complex) have been reported. There is an urgent need for DNA-based markers to explore the epidemiology of these emerging fusarioid pathogens using molecular methods. We took advantage of fusarioid genomes available in NCBI (n = 20) to optimize the development of novel amplified fragment length polymorphism (AFLP) markers by conducting in-depth &lt;i&gt;in silico&lt;/i&gt; analyses to refine their applicability for studies on these pathogens' genetic epidemiology. &lt;i&gt;In-silico&lt;/i&gt; screening highlighted eight primer pair combinations (C1-C8) to be tested &lt;i&gt;in vitro&lt;/i&gt;. The AFLP protocol was used for genotyping 40 medically relevant fusarioid fungi. Based on the overall scored AFLP markers (77-93 fragments), the values of polymorphism information content (&lt;i&gt;PIC&lt;/i&gt; = 0.3474-0.3725), marker index (&lt;i&gt;MI&lt;/i&gt; = 0.0038-0.0056), effective multiplex ratio (&lt;i&gt;E&lt;/i&gt; = 26.3750-40.4750), resolving power (&lt;i&gt;Rp&lt;/i&gt; = 40.1500-54.6000), discriminating power (&lt;i&gt;D&lt;/i&gt; = 0.7978-0.8857), expected heterozygosity (&lt;i&gt;H&lt;/i&gt; = 0.4476-0.4949), and mean heterozygosity (&lt;i&gt;H&lt;/i&gt; &lt;sub&gt;avp&lt;/sub&gt; = 0.0001) demonstrated the utility of these primer combinations for discriminating &lt;i&gt;Neocosmospora, Fusarium s. str&lt;/i&gt;., and &lt;i&gt;Bisifusarium&lt;/i&gt; species. Of relevance, some AFLP panels were better than others at studying genetic trends in &lt;i&gt;Neocosmospora&lt;/i&gt; (#2 EcoRI-AT/MseI-TA, #3 EcoRI-AA/MseI-TT, and #5 EcoRI-AT/MseI-AG) or &lt;i&gt;Fusarium s. str.&lt;/i&gt; (mainly #2 EcoRI-AT/MseI-TA and #6 EcoRI-GA/MseI-TT) and &lt;i&gt;Bisifusarium&lt;/i&gt; (#1 EcoRI-GA/MseI-AG and #6 EcoRI-GA/MseI-TT), and these combinations will better resolve disease transmission routes. Our DNA fingerprint assay has proven effective by exhibiting rapidity, reproducibility, and high discriminatory capabilities, which represents a valuable asset in the ongoing efforts to combat fusariosis and enhance our scientific understanding of medically relevant &lt;i&gt;Fusarium&lt;/i&gt; and allied fusarioid genera. &lt;b&gt;Citation:&lt;/b&gt; Monteiro RC, Yu CZ, Dolatabadi S, Hagen F, Sandoval-Denis M, Crous PW, Fisher MC, Gonçalves SS, de Camargo ZP, Hofling-Lima AL, Rodrigues AM (2025). Novel amplified fragment length polymorphism (AFLP) markers for typing medically relevant &lt;i&gt;Fusari","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"15 ","pages":"79-96"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11959234/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143765916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Cortinarius spilomeoalpinus</i> (<i>Basidiomycota</i>, <i>Agaricales</i>) a new alpine species of sect. <i>Spilomei</i> from South-Tyrolean <i>Dryas octopetala</i> <b>habitats</b>.","authors":"U Peintner, L Huymann, R J Ferrari","doi":"10.3114/fuse.2025.15.12","DOIUrl":"10.3114/fuse.2025.15.12","url":null,"abstract":"<p><p>During our long-term studies on <i>Cortinarius</i> species in alpine habitats, we discovered several collections of a <i>Cortinarius</i> species resembling <i>C. spilomeus</i>. We carried out comparative morphological studies and a phylogenetic analysis based on rDNA ITS sequences. We included also type material of <i>Cortinarius spilomeus forma dryadicola</i>. We confirmed that <i>Cortinarius spilomeoalpinus</i> is a distinct species which is typical for alpine <i>Dryas octopetala</i> habitats. <i>Cortinarius spilomeus forma dryadicola</i> is not part of <i>C. spilomeus</i> sensu stricto. It is conspecific with the later described <i>C. ferrusinus</i>, another taxon of sect. <i>Spilomei</i>, and we treat it as <i>forma</i> of it. Detailed descriptions are given for the alpine taxa, and a differential diagnosis and dichotomous identification key are provided. <b>Citation:</b> Peintner U, Huymann L, Ferrari RJ (2025). <i>Cortinarius spilomeoalpinus</i> (<i>Basidiomycota, Agaricales</i>) a new alpine species of sect. <i>Spilomei</i> from the South-Tyrolean <i>Dryas octopetala</i> habitats<i>. Fungal Systematics and Evolution</i> <b>15</b>: 265-274. doi: 10.3114/fuse.2025.15.12.</p>","PeriodicalId":73121,"journal":{"name":"Fungal systematics and evolution","volume":"15 ","pages":"265-274"},"PeriodicalIF":0.0,"publicationDate":"2025-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11952185/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143756272","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}