Frontiers in fungal biology最新文献

{"title":"Morphological variations and adhesive distribution: a cross-species examination in <i>Colletotrichum</i> conidia.","authors":"Caleb Oliver Bedsole, Mary Cowser, Timothy Martin, Jillian Hamilton, Lucia Gonzalez Rodriguez, Thomas M Chappell, Brian D Shaw","doi":"10.3389/ffunb.2024.1481865","DOIUrl":"10.3389/ffunb.2024.1481865","url":null,"abstract":"<p><p><i>Colletotrichum</i> is a globally significant genus of plant pathogens known for causing anthracnose across a diverse array of hosts. Notably, <i>Colletotrichum graminicola</i> is a pathogen affecting maize. Annually, the global economic impact of this pathogen reaches billions of US dollars. <i>C. graminicola</i> produces conidia that have a characteristic falcate shape and are dispersed by rain. Upon attachment to maize leaves, these conidia develop melanized appressoria to penetrate the leaf surface to initiate disease. Recent findings have emphasized the existence of an adhesive strip on only one side of <i>C. graminicola</i> conidia. This strip colocalizes with an actin array, playing a crucial role in facilitating attachment and germination. This asymmetrical adhesive was postulated to enhance spore dispersal by assuring that some conidia do not attach to their initial deposition site. The extent of this asymmetric adhesive phenotype in other <i>Colletotrichum</i> species remains unknown, raising questions about its conservation within the genus. This study reveals the ubiquitous presence of an asymmetric adhesive on the conidia across nine isolates of <i>Colletotrichum</i>, representing eight species. Morphological differences in conidium shape and adhesive distribution were observed. Significantly, <i>Colletotrichum truncatum</i> is unique from other observed species by exhibiting an adhesive strip on both sides of its conidium. Furthermore, in <i>C. graminicola</i>, we noted a simultaneous development of the actin array and detachment from its mother cell after spore development. We posit that the study of other <i>Colletotrichum</i> members holds promise in elucidating the evolutionary trajectory of this phenotype. Furthermore, these insights may prove instrumental in understanding spore dispersal dynamics across diverse hosts, shedding light on the intricate web of host specificity within the genus.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"5 ","pages":"1481865"},"PeriodicalIF":2.1,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11671520/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142904130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Three-year multi-mycotoxin analysis of South African commercial maize from three provinces.","authors":"Queenta Ngum Nji, Mulunda Mwanza","doi":"10.3389/ffunb.2024.1426782","DOIUrl":"10.3389/ffunb.2024.1426782","url":null,"abstract":"<p><strong>Introduction: </strong>The Food and Agricultural Organization (FAO) reported that numerous diseases can be traced back to the consumption of unsafe food contaminated with mycotoxins. Mycotoxins are secondary metabolites produced by toxigenic filamentous fungi. Mycotoxins reported to be of socio-economic concerns include aflatoxins, fumonisins, zearalenone, ochratoxin A, and deoxynivalenol. These mycotoxins are frequent contaminants of maize especially in the face of climate change and global food insecurity. South Africa is a leading exporter of maize in Africa, hence, it is crucial to evaluate exposure risks with respect to mycotoxin contamination of maize for consumers' safety.</p><p><strong>Materials and method: </strong>In total, 752 post-harvest maize samples collected from silos over a 3-year period were analysed using liquid chromatography with tandem mass spectrometry (LC-MS/MS) for the occurrence of mycotoxins.</p><p><strong>Results and discussion: </strong>The overall mean values for all the quantified mycotoxins were within the South Africa regulatory limit as well as the individual samples, apart from DON and FB mycotoxins with 5% and 1% samples, respectively, above the limit. Citrinin was quantified in South African commercial maize for the first time. The presence of major mycotoxins in South African commercial maize even within safety limits is of public health concern, hence, continuous monitoring and evaluation is recommended.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"5 ","pages":"1426782"},"PeriodicalIF":2.1,"publicationDate":"2024-12-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11647527/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biological evaluation of semi-synthetic isoindolinone isomers produced by <i>Stachybotrys chartarum</i>.","authors":"Alica Fischle, Ulrich Schreiber, Viola Haupt, Felix Schimang, Lina Schürmann, Matthias Behrens, Florian Hübner, Melanie Esselen, Dmitrii V Kalinin, Svetlana A Kalinina","doi":"10.3389/ffunb.2024.1494795","DOIUrl":"10.3389/ffunb.2024.1494795","url":null,"abstract":"<p><p>The filamentous fungus <i>Stachybotrys chartarum</i> is rich in meroterpenoid secondary metabolites, some of which carry <i>o</i>-dialdehyde moieties, which are readily derivatized to isoindolinones by addition of primary amines. The structural diversity of phenylspirodrimanes, in particular, is linked to a wide range of biological activities, making them ideal candidates for semi-synthetic modification. In this study, acetoxystachybotrydial acetate was reacted with l-tryptophan and tryptamine, resulting in the detection of both regiospecific isomeric structures - a rare and significant finding that enabled the examination of four novel reaction products. Besides their successful purification, a detailed report on their isomer-specific behavior with regard to chromatographic retention, UV-spectral specificities, nuclear magnetic resonances, and mass spectrometric fragmentation is given. Furthermore, a comprehensive insight into each compounds' unique effect within the tested biological assays is provided, which include cytotoxicity, genotoxicity, their biological activity against serine proteases of the blood coagulation cascade, and <i>in vitro</i> hepatic metabolism, always in comparison to the non-derivatized substance. Ultimately, each isomer can be distinguished already during the purification process, which extends to the biological assays where we present one less cytotoxic, faster metabolized, and more active regio-isomeric phenylspirodrimane-derivative.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"5 ","pages":"1494795"},"PeriodicalIF":2.1,"publicationDate":"2024-11-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11621054/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142803680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"General aspects, host interaction, and application of <i>Metarhizium</i> sp. in arthropod pest and vector control.","authors":"Rodrigo Prado Rodrigues de Miranda, Talita Kellen Dos Anjos Soares, Daniele Pereira Castro, Fernando Ariel Genta","doi":"10.3389/ffunb.2024.1456964","DOIUrl":"10.3389/ffunb.2024.1456964","url":null,"abstract":"<p><p>The application of microorganisms as bio-control agents against arthropod populations is a need in many countries, especially in tropical, subtropical, and neotropical endemic areas. Several arthropod species became agricultural pests of paramount economic significance, and many methods have been developed for field and urban applications to prevent their, the most common being the application of chemical insecticides. However, the indiscriminate treatment based upon those substances acted as a selective pressure for upcoming resistant phenotype populations. As alternative tools, microorganisms have been prospected as complementary tools for pest and vectorial control, once they act in a more specific pattern against target organisms than chemicals. They are considered environmentally friendly since they have considerably less off-target effects. Entomopathogenic fungi are organisms capable of exerting pathogenesis in many vector species, thus becoming potential tools for biological management. The entomopathogenic fungi <i>Metarhizium</i> sp. have been investigated as a microbiological agent for the control of populations of insects in tropical regions. However, the development of entomopathogenic fungi as control tools depends on physiological studies regarding aspects such as mechanisms of pathogenicity, secreted enzymes, viability, and host-pathogen aspects. The following review briefly narrates current aspects of entomopathogenic fungi, such as physiology, cellular characteristics, host-pathogen interactions, and its previous applications against different insect orders with medical and economic importance. Approaches integrating new isolation, prospection, characterization, delivery strategies, formulations, and molecular and genetic tools will be decisive to elucidate the molecular mechanisms of EPFs and to develop more sustainable alternative pesticides.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"5 ","pages":"1456964"},"PeriodicalIF":2.1,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11614621/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142781922","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial: Predicting, managing, and minimizing mycotoxicosis in farm animals.","authors":"Jesús Adonai Maguey-González, Juan D Latorre, Sergio Gomez-Rosales, Abraham Mendez-Albores","doi":"10.3389/ffunb.2024.1519411","DOIUrl":"https://doi.org/10.3389/ffunb.2024.1519411","url":null,"abstract":"","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"5 ","pages":"1519411"},"PeriodicalIF":2.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11604792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142775082","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Corrigendum: Navigating the fungal battlefield: cysteine-rich antifungal proteins and peptides from Eurotiales.","authors":"Jeanett Holzknecht, Florentine Marx","doi":"10.3389/ffunb.2024.1511041","DOIUrl":"https://doi.org/10.3389/ffunb.2024.1511041","url":null,"abstract":"<p><p>[This corrects the article DOI: 10.3389/ffunb.2024.1451455.].</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"5 ","pages":"1511041"},"PeriodicalIF":2.1,"publicationDate":"2024-11-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11587351/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142717850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An overview of symbiotic and pathogenic interactions at the fungi-plant interface under environmental constraints.","authors":"Sunishtha Mishra, Anukriti Srivastava, Ajeet Singh, Girish Chandra Pandey, Garima Srivastava","doi":"10.3389/ffunb.2024.1363460","DOIUrl":"https://doi.org/10.3389/ffunb.2024.1363460","url":null,"abstract":"<p><p>The complex and dynamic interactions between fungi and plants constitute a critical arena in ecological science. In this comprehensive review paper, we explore the multifaceted relationships at the fungi-plant interface, encompassing both mutualistic and antagonistic interactions, and the environmental factors influencing these associations. Mutualistic associations, notably mycorrhizal relationships, play a pivotal role in enhancing plant health and ecological balance. On the contrary, fungal diseases pose a significant threat to plant health, agriculture, and natural ecosystems, such as rusts, smuts, powdery mildews, downy mildews, and wilts, which can cause extensive damage and lead to substantial economic losses. Environmental constraints encompassing abiotic and biotic factors are elucidated to understand their role in shaping the fungi-plant interface. Temperature, moisture, and soil conditions, along with the presence of other microbes, herbivores, and competing plants, significantly influence the outcome of these interactions. The interplay between mutualism and antagonism is emphasised as a key determinant of ecosystem health and stability. The implications of these interactions extend to overall ecosystem productivity, agriculture, and conservation efforts. The potential applications of this knowledge in bioremediation, biotechnology, and biocontrol strategies emphasise the importance of adapting to climate change. However, challenges and future directions in this field include the impacts of climate change, emerging fungal pathogens, genomic insights, and the role of the fungi-plant interface in restoration ecology. Hence, this review paper provides a comprehensive overview of fungi-plant interactions, their environmental influences, and their applications in agriculture, conservation, and ecological restoration.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"5 ","pages":"1363460"},"PeriodicalIF":2.1,"publicationDate":"2024-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11544544/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142633487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metal tolerance of Río Tinto fungi.","authors":"Monike Oggerin, Catalina Del Moral, Nuria Rodriguez, Nuria Fernandez-Gonzalez, José Manuel Martínez, Iván Lorca, Ricardo Amils","doi":"10.3389/ffunb.2024.1446674","DOIUrl":"10.3389/ffunb.2024.1446674","url":null,"abstract":"<p><p>Southwest Spain's Río Tinto is a stressful acidic microbial habitat with a noticeably high concentration of toxic heavy metals. Nevertheless, it has an unexpected degree of eukaryotic diversity in its basin, with a high diversity of fungal saprotrophs. Although some studies on the eukaryotic diversity in Rio Tinto have been published, none of them used molecular methodologies to describe the fungal diversity and taxonomic affiliations that emerge along the river in different seasons. The aim of the present study was to isolate and describe the seasonal diversity of the fungal community in the Río Tinto basin and its correlation with the physicochemical parameters existing along the river's course. The taxonomic affiliation of 359 fungal isolates, based on the complete internal transcribed spacer DNA sequences, revealed a high degree of diversity, identifying species belonging primarily to the phylum Ascomycota, but representatives of the Basidiomycota and Mucoromycota phyla were also present. In total, 40 representative isolates along the river were evaluated for their tolerance to toxic heavy metals. Some of the isolates were able to grow in the presence of 1000 mM of Cu²⁺, 750 mM of As⁵⁺ and Cd²⁺, and 100 mM of Co²⁺, Ni²⁺, and Pb²⁺.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"5 ","pages":"1446674"},"PeriodicalIF":2.1,"publicationDate":"2024-10-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11521807/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"What lies behind the large genome of <i>Colletotrichum lindemuthianum</i>.","authors":"Leandro Lopes da Silva, Hilberty Lucas Nunes Correia, Osiel Silva Gonçalves, Pedro Marcus Pereira Vidigal, Rafael Oliveira Rosa, Mateus Ferreira Santana, Marisa Vieira de Queiroz","doi":"10.3389/ffunb.2024.1459229","DOIUrl":"https://doi.org/10.3389/ffunb.2024.1459229","url":null,"abstract":"<p><p><i>Colletotrichum lindemuthianum</i> is the etiological agent of anthracnose disease in common bean (<i>Phaseolus vulgaris</i> L.), noted for its ability to cause serious damage and significant pathogenic variability. This study reveals the features of the high-quality genome of <i>C. lindemuthianum</i>. Analysis showed improvements over the first assembly, with the refined genome having 119 scaffolds, ten times fewer than the first, and a 19% increase in gene number. The effector candidates increased by nearly 1.5 times. More than 40% of the amino acid sequences with homologs in the Pathogen-Host Interactions (PHI-base) database are linked to pathogenicity. Of 18 putative proteins identified as Chitinase-like Protein, six have a mutation in the enzyme catalytic motif, and three showed gene expression in the biotrophic phase, indicating they can act as effectors. Comparative genomic analyses with 30 other fungal species revealed that <i>C. lindemuthianum</i> is among the top three fungi encoding transport proteins. Seven Necrosis and Ethylene-Inducing Peptide 1 (Nep1)-Like Proteins (NLPs) are present in the <i>C. lindemuthianum</i> genome, but none had complete identity with the GHRHDWE conserved motif of NLPs; two were grouped with proteins that induce necrosis and may retain the capability to induce host necrosis. <i>Colletotrichum</i> species show a high number of secondary metabolite (SM) clusters, with <i>C. lindemuthianum</i> having 47 SM clusters. Approximately 60% of the <i>C. lindemuthianum</i> genome is composed of repetitive elements, a significantly higher proportion than in other fungi. These differences in transposable element (TE) numbers may explain why <i>C. lindemuthianum</i> has one of the largest genomes among the fungi analyzed. A significant portion of its genome comprises retroelements, particularly the <i>Ty1/Copia</i> superfamily, which accounts for 22% of the genome and represents 40% of the repetitive elements. The genomic profile features a remarkably high RIP-affected genomic proportion of 54.77%, indicating substantial RIP activity within this species. This high-quality genome of <i>C. lindemuthianum</i>, a significant pathogen in common bean cultivation, will support future research into this pathosystem, fostering a deeper understanding of the interaction between the fungus and its host.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"5 ","pages":"1459229"},"PeriodicalIF":2.1,"publicationDate":"2024-10-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11518743/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142549256","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Conserved perception of host and non-host signals via the a-pheromone receptor Ste3 in <i>Colletotrichum graminicola</i>.","authors":"Anina Yasmin Rudolph, Carolin Schunke, Daniela Elisabeth Nordzieke","doi":"10.3389/ffunb.2024.1454633","DOIUrl":"10.3389/ffunb.2024.1454633","url":null,"abstract":"<p><p>Understanding the interactions between fungal plant pathogens and host roots is crucial for developing effective disease management strategies. This study investigates the molecular mechanisms underpinning the chemotropic responses of the maize anthracnose fungus <i>Colletotrichum graminicola</i> to maize root exudates. Combining the generation of a deletion mutant with monitoring of disease symptom development and detailed analysis of chemotropic growth using a 3D-printed device, we identify the 7-transmembrane G-protein coupled receptor (GPCR) CgSte3 as a key player in sensing both plant-derived class III peroxidases and diterpenoids. Activation of CgSte3 initiates signaling through CgSo, a homolog to the Cell Wall Integrity Mitogen-Activated Protein Kinase (CWI MAPK) pathway scaffold protein identified in other filamentous fungi, facilitating the pathogen's growth towards plant defense molecules. The NADPH oxidase CgNox2 is crucial for peroxidase sensing but not for diterpenoid detection. These findings reveal that CgSte3 and CWI MAPK pathways are central to <i>C. graminicola's</i> ability to hijack plant defense signals, highlighting potential targets for controlling maize anthracnose.</p>","PeriodicalId":73084,"journal":{"name":"Frontiers in fungal biology","volume":"5 ","pages":"1454633"},"PeriodicalIF":2.1,"publicationDate":"2024-10-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11491335/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142482331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}