Frontiers in bioscience (Elite edition)最新文献

{"title":"Peri-Implant Mucositis Treatment with a Chlorexidine Gel with A.D.S. 0.5%, PVP-VA and Sodium DNA vs a Placebo Gel: A Randomized Controlled Pilot Clinical Trial.","authors":"Felice Lorusso,&nbsp;Gianluca Tartaglia,&nbsp;Francesco Inchingolo,&nbsp;Antonio Scarano","doi":"10.31083/j.fbe1404030","DOIUrl":"https://doi.org/10.31083/j.fbe1404030","url":null,"abstract":"<p><strong>Background: </strong>Peri-implant mucositis is a bacterial and inflammatory disease of implant surrounding soft tissues. Chlorhexidine (CHX) associated with PVP-VA (Polyvinylpyrrolidone/vinyl acetate) has an adjuvant function in the antibacterial activity: it protects the film-forming layer, due to polymer, from microbial contamination or over-infection, and prolongs its permanence, without a direct action on the tissues and wounds. Sodium DNA is able to improve gingival trophism. The aim of the study was to compare a gel with CHX and antidiscoloration system (A.D.S.) 0.5% with the addition of PVP-VA and sodium DNA with a placebo gel without chlorexidine in peri-implant mucositis treatment.</p><p><strong>Methods: </strong>Trial design: A single center randomized controlled pilot clinical trial was conducted on a total of 24 patients with mucositis, randomly allocated into two groups. In the group (A) (n = 12) patients were treated with a gel with CHX + A.D.S. 0.5% while a placebo gel was used in the group (B) (n = 12).</p><p><strong>Outcome: </strong>Plaque Index (PI) (primary outcome), Bleeding on probing (BOP) and Gingival Index (GI) (secondary outcomes) were assessed at baseline and at 2 weeks after treatment period.</p><p><strong>Results: </strong>Mean PI score recorded at baseline was 2.4 ± 0.4 [95% CI: -2.682/7.482] in the group (A) and 2.2 ± 0.5 [95% CI: -4.153/8.553] in the group (B) (<i>p</i> > 0.05) while mean PI score at 2 weeks was 0.5 ± 0.4 [95% CI: -4.582/5.582] and 1.7 ± 1.9 [95% CI: -9.736/13.14] in the group and in the group respectively (<i>p</i> < 0.05). Mean BOP value at baseline was 57.1 ± 15.2% [95% CI: -136.0/250.2] in the group (A) and 55.3% ± 11.7% [95% CI: -93.36/204.0] (<i>p</i> > 0.05) while mean BOP value at 2 weeks follow-up was 14.3% ± 6.6% [95% CI: -69.56/98.16] (A) and 45.4% ± 9.8% [95% CI: -79.12/169.9] (B) respectively (<i>p</i> < 0.05).</p><p><strong>Conclusions: </strong>A significant reduction in gingival inflammation indexes was found at 2 weeks in patients with peri-implant mucositis treated with a gel with CHX + A.D.S. 0.5% with the addition of PVP-VA and sodium DNA compared to those treated with a placebo gel.</p><p><strong>Clinical trial registration: </strong>2019-004976-20.</p>","PeriodicalId":73068,"journal":{"name":"Frontiers in bioscience (Elite edition)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10502315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding the Feedback Loops between Energy, Matter and Life.","authors":"Richard H W Funk","doi":"10.31083/j.fbe1404029","DOIUrl":"https://doi.org/10.31083/j.fbe1404029","url":null,"abstract":"<p><p>This review gathers recent findings in biophysics that shed light on the biological principle of self-organization, spanning from molecules to more complicated systems with higher information processing capacity. The focus is on \"feedback loops\" from information and matter to an exchange component with a more fundamental meaning than \"cybernetic regulation\" and \"maintenance of homeostasis\". This article proposes that electric and electromagnetic forces are the most important mediators over large distances. Field-like mediation is distinguished from cell-to-cell communication by special electric- or ion-guiding mechanisms that create additional pathways to the \"classical\" mediators such as nerve conduction or blood flow. Resonance phenomena from phonons and photons in the visible range will be discussed in relation to organelles, cytoskeletal elements and molecules. In this context, the aqueous surrounding of molecules and cells is an important aspect. Many of these phenomena are caused by quantum physics, such as the tunneling of electrons in enzymes or in other coherent working systems. This suggests that quantum information processing is also spread over large-scale areas of an organism.</p>","PeriodicalId":73068,"journal":{"name":"Frontiers in bioscience (Elite edition)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10553205","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Technology as a Way of Cleaning the Environment from Petroleum Substances in South-Eastern Poland.","authors":"Barbara Sawicka,&nbsp;Viola Vambol,&nbsp;Barbara Krochmal-Marczak,&nbsp;Mohammed Messaoudi,&nbsp;Dominika Skiba,&nbsp;Piotr Pszczółkowski,&nbsp;Piotr Barbaś,&nbsp;Alaa K Farhan","doi":"10.31083/j.fbe1404028","DOIUrl":"https://doi.org/10.31083/j.fbe1404028","url":null,"abstract":"<p><strong>Background: </strong>In phytoextraction methods, the problem is the obtained contaminated plant biomass, the selection of the appropriate species, resistant to the type and degree of contamination, as well as the long time needed to completely clean the soil.</p><p><strong>Goal: </strong>when selecting the appropriate method of remediation of soils contaminated with polycyclic aromatic hydrocarbons, not only the effectiveness of the method should be considered, but also the degree of contamination, the location of the site and its current and planned use.</p><p><strong>Methods: </strong>Descriptive, laboratory and comparative methods were used.</p><p><strong>Results: </strong>Soil contamination with polycyclic aromatic hydrocarbons (PAHs), which can cause mutations and cancer, is of particular concern as it affects not only human health but also vegetation growth and the biological environment. A fast, nature-friendly and cost-effective method is required to remove and minimize the hazardous effects of crude oil.</p><p><strong>Conclusions: </strong>Green technology is particularly beneficial, especially the phytoextraction technique, in which plants clean the soil of excess petroleum products, prevent its further movement from the site of contamination and prevent erosion of reclaimed soil. Species such as: <i>Trifolium repens</i>, <i>Trifolium pratense</i>, <i>Lotus corniculatus</i>, <i>Agrostis stolonifera</i>, <i>Festuca rubra</i> subsp. <i>trichophylla</i>, <i>Arrhenatherum elatius</i> performed their tasks very well, therefore they can be recommended for use as a factor counteracting environmental degradation.</p>","PeriodicalId":73068,"journal":{"name":"Frontiers in bioscience (Elite edition)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10553203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural and Semi-Synthetic Flavonoid Anti-SARS-CoV-2 Agents for the Treatment of Long COVID-19 Disease and Neurodegenerative Disorders of Cognitive Decline.","authors":"James Melrose,&nbsp;Margaret M Smith","doi":"10.31083/j.fbe1404027","DOIUrl":"https://doi.org/10.31083/j.fbe1404027","url":null,"abstract":"The aim of this review is to highlight the beneficial attributes of flavonoids, a diverse family of widely-distributed polyphenolic phytochemicals that have beneficial cell and tissue protective properties. Phytochemicals are widely distributed in plants, herbs and shrubs used in traditional complimentary medical formulations for centuries. The bioactive components that convey beneficial medicinal effects in these complex herbal preparations are now being identified using network pharmacology and molecular docking procedures that identify their molecular targets. Flavonoids have anti-oxidant, anti-inflammatory, antiviral, antibacterial and anti-cancer properties that have inspired the development of potent multifunctional derivatised flavonoids of improved efficacy. The antiviral properties of flavonoids and the emergence of the severe acute respiratory syndrome (SARS-CoV-2) pandemic has resulted in a resurgence of interest in phytochemicals in the search for efficacious compounds that can prevent viral infection or replication, with many promising plant compounds identified. Promising semi-synthetic flavonoid derivatives have also been developed that inhibit multiple pathological neurodegenerative processes; these offer considerable promise in the treatment of diseases of cognitive decline. Clinical trials are currently being undertaken to evaluate the efficacy of dietary supplements rich in flavonoids for the treatment of virally-mediated diseases. Such trials are expected to identify flavonoids with cell and tissue protective properties that can be harnessed in biomedical applications that may serve as supportive adjunctive procedures to conventional anti-viral drug therapies against diseases such as COVID-19.","PeriodicalId":73068,"journal":{"name":"Frontiers in bioscience (Elite edition)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10553204","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Proximate Composition, Physicochemical and Microbiological Characterization of Edible Seaweeds Available in the Portuguese Market.","authors":"Bruno Miguel Campos,&nbsp;Edgar Ramalho,&nbsp;Isa Marmelo,&nbsp;João Paulo Noronha,&nbsp;Manuel Malfeito-Ferreira,&nbsp;Paulina Mata,&nbsp;Mário Sousa Diniz","doi":"10.31083/j.fbe1404026","DOIUrl":"https://doi.org/10.31083/j.fbe1404026","url":null,"abstract":"<p><strong>Background: </strong>The aim of this work was the study of the proximate composition and profile of fatty acids, minerals, and some microbiological aspects of four edible seaweed species (<i>Chondrus crispus</i>, <i>Palmaria palmata</i>, <i>Porphyra</i> sp., and <i>Ulva</i> sp.) available in the Portuguese market for food consumption, and produced in a national Integrated Multi-Trophic System (IMTA).</p><p><strong>Methods: </strong>Moisture, ash, and total lipids were determined gravimetrically. Crude protein was analysed by Duma's combustion procedures. The total carbohydrate content was assayed by the phenol/sulphuric acid method. The assessment of the fatty acids methyl esters (FAMEs) was determined through GC-MS. Characterization of elemental analysis was performed by ICP-AES. Different standard microbiological methods were applied for microorganisms. Statistics were performed using the non-parametric Mann-Whitney U test to assess significant differences between samples.</p><p><strong>Results: </strong>Lipid contents (<i>n</i> = 3) were very low (1.6-2.3%), particularly in <i>Palmaria palmata</i>, and <i>Chondrus crispus</i> (1.6-1.7%). The protein content (<i>n</i> = 4) varied from 14.4% in <i>P. palmata</i> to 23.7% in <i>Porphyra</i> sp. Carbohydrates (<i>n</i> = 3) were the major constituent of most seaweeds (31-34%), except in <i>Porphyra</i> sp., with higher content in proteins than carbohydrates. Regarding the fatty acid content (<i>n</i> = 4), in general, saturated fatty acids (SFAs) were the most abundant followed by polyunsaturated fatty acids (PUFAs) and monounsaturated fatty acids (MUFAs). Among macro and trace elements (<i>n</i> = 3), <i>Chondrus crispus</i> shows the highest average content in Zn (71.1 mg ⋅ kg - 1 D.W.), <i>Palmaria palmata</i> the highest average content in K (124.8 g ⋅ kg - 1 D.W.), <i>Porphyra</i> sp. the highest average content in P (2.1 g ⋅ kg - 1 D.W.), and <i>Ulva</i> sp. the highest average content of Ca (5.5 g ⋅ kg - 1 D.W.), Mg (55.8 g ⋅ kg - 1 D.W.), and Fe (336.3 mg ⋅ kg - 1 D.W.). In general, Na and K were the most abundant elements among analysed seaweed. Additionally, the microbiological results (<i>n</i> = 4) comply with the Portuguese guidelines (subgroup 2D) on the application of general principles of food hygiene in ready-to-eat foods.</p><p><strong>Conclusions: </strong>Overall, the results highlight the potential of using these seaweeds as an alternative and sustainable source of elements and bioactive compounds to produce enriched food products with a beneficial potential for human nutrition.</p>","PeriodicalId":73068,"journal":{"name":"Frontiers in bioscience (Elite edition)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10496015","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Antimicrobial and Antibiotic-Resistance Reversal Activity of Some Medicinal Plants from Cameroon against Selected Resistant and Non-Resistant Uropathogenic Bacteria.","authors":"Mbarga Manga Joseph Arsene,&nbsp;Podoprigora Irina Viktorovna,&nbsp;Anyutoulou Kitio Linda Davares,&nbsp;Kezimana Parfait,&nbsp;Smolyakova Larissa Andreevna,&nbsp;Hippolyte Tene Mouafo,&nbsp;Manar Rehailia,&nbsp;Yashina Natalia Vyacheslavovna,&nbsp;Smirnova Irina Pavlovna,&nbsp;Irma Aurelia Monique Manga,&nbsp;Das Milana Sergueïevna","doi":"10.31083/j.fbe1404025","DOIUrl":"https://doi.org/10.31083/j.fbe1404025","url":null,"abstract":"<p><strong>Background and aim: </strong>Antibiotics' resistance is the leading cause of complications in the treatment of urinary tract infections. This study aimed to screen the antimicrobial potential of 8 plants from Cameroon against multi-resistant uropathogenic (MRU) bacteria and to investigate their antibioresistance reversal properties.</p><p><strong>Method: </strong>Bioactive compounds were extracted from leaves of <i>Leucanthemum vulgare</i>, <i>Cymbopogon citratus</i>, <i>Moringa oleifera</i> and <i>Vernonia amygdalina</i>; barks of <i>Cinchona officinalis</i> and <i>Enantia chlorantha</i> barks and seeds of <i>Garcinia lucida</i> and leaves and seeds of <i>Azadirachta indica</i> using water and ethanol as solvents. The extracts were tested against <i>Escherichia coli</i> ATCC 25922, <i>Staphylococcus aureus</i> ATCC 6538 and <i>Candida albicans</i> 10231 using the well diffusion and the broth microdilution methods. The antibiotic-resistance reversal activity was assessed against selected MRU bacteria. The phytochemical composition and the elemental composition of the most active extracts were assessed respectively using HPLC-MS/MS and X-ray fluorescence (XRF) spectrometry.</p><p><strong>Results: </strong>Among the most active plants, in decreasing order of antimicrobial activity we found ethanolic (EE) and aqueous extracts (AE) of <i>E. chloranta</i> bark (ECB), EE of <i>L. vulgare</i> leaves and <i>G. lucida</i> seeds. The best synergies between common antibiotics and extracts were found with EE-ECB which well-modulated kanamycin nitrofurantoin and ampicillin. All the compounds identified in EE-ECB were alkaloids and the major constituents were palmatine (51.63%), columbamine+7,8-dihydro-8-hydroxypalmatine (19.21%), jatrorrhizine (11.02%) and pseudocolumbamine (6.33%). Among the minerals found in EE-ECB (S, Si, Cl, K, Ca, Mn, Fe, Zn and Br), Br, Fe and Cl were the most abundant with mean fluorescence intensities of 4.6529, 3.4854 and 2.5942 cps/uA respectively.</p><p><strong>Conclusions: </strong>The ethanol extract of the bark of <i>E. chlorantha</i> has remarkable, broad-spectrum antimicrobial and contains several palmatine derivatives.</p>","PeriodicalId":73068,"journal":{"name":"Frontiers in bioscience (Elite edition)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10496016","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cuminaldehyde ameliorates hyperglycemia in diabetic mice.","authors":"Kannan Duraisamy,&nbsp;Pari Leelavinothan,&nbsp;Paari Ellappan,&nbsp;Tulsi Deepak Singh Balaji,&nbsp;Ponnulakshmi Rajagopal,&nbsp;Selvaraj Jayaraman,&nbsp;Shyamaldevi Babu","doi":"10.31083/j.fbe1404024","DOIUrl":"https://doi.org/10.31083/j.fbe1404024","url":null,"abstract":"<p><strong>Background: </strong>Animal-fats are rich in long-chain saturated fatty-acids, well known to induct diabetic distress among ingested insulin-insensitive individuals. In the current-study, bovine-fat was fed to selective mice breeds highly sensitized to heavy dietary lipid load.</p><p><strong>Methods: </strong>The later high fat diet (HFD) group indeed undergone diabetic-onset within weeks with a drastically altered feed-behavior pattern. It consumed more food, gained body mass, elevated homeostatic model assessment value and extensively glycosylated Hb transporters.</p><p><strong>Results: </strong>However, the hypothetical test drug (Cuminaldehyde or CA) with known therapeutic-potential worked-well to balance food efficiency-ratio and Hb- counts closer to control. The fat-soluble phytochemical mono-terpenoid (CA) promoted constitutive mono-hexose (glucose) consuming catabolic-cycles via mono-glycoprotein (insulin) signal-transduction. It resolved diabetogenic-upsurge of gluconeogenic-enzymes, reduced non-sugar (amino/fatty acids) utilization by restricting transamination/dephosphorylation and restored liver-glycogen reserves near to normal-group effectively at 10 mg/kg b.w dose.</p><p><strong>Conclusions: </strong>Hence, the nutraceutical-potential (anti-diabetes/transaminitis ability) of administered exogenous redox-active agent CA can be entertained for evoking therapeutic-heath in diabetic human-community.</p>","PeriodicalId":73068,"journal":{"name":"Frontiers in bioscience (Elite edition)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-09-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10496014","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"<i>Abiotrophia</i> spp. and <i>Granulicatella</i> spp. Infective Endocarditis: A Contemporary Perspective.","authors":"Agustín Estévez,&nbsp;Mercedes Marín,&nbsp;Carlos Sánchez-Carrillo,&nbsp;Marina Machado,&nbsp;Luís Alcalá,&nbsp;Blanca Pinilla,&nbsp;Antonia Delgado,&nbsp;Víctor González-Ramallo,&nbsp;Álvaro Pedraz,&nbsp;Manuel Martínez-Sellés,&nbsp;Emilio Bouza,&nbsp;Maricela Valerio,&nbsp;Patricia Muñoz,&nbsp;On Behalf Of Game-Hgugm","doi":"10.31083/j.fbe1403023","DOIUrl":"https://doi.org/10.31083/j.fbe1403023","url":null,"abstract":"<p><strong>Background: </strong><i>Abiotrophia</i> spp. and <i>Granulicatella</i> spp. are Gram-positive cocci, formerly known as nutritionally variant or deficient <i>Streptococcus</i>. Their role as causative agents of infective endocarditis (IE) is numerically uncertain, as well as diagnostic and clinical management of this infection. The aim of our study is to describe the clinical, microbiological, therapeutic, and prognosis of patients with IE caused by these microorganisms in a large microbiology department.</p><p><strong>Methods: </strong>Retrospective analysis of all the patients with <i>Abiotrophia</i> spp. and <i>Granulicatella</i> spp. IE registered in our centre in the period 2004-2021.</p><p><strong>Results: </strong>Of the 822 IE in the study period, 10 (1.2%) were caused by <i>Abiotrophia</i> spp. (7) or <i>Granulicatella</i> spp. (3). The species involved were <i>A.defectiva</i> (7), <i>G.adiacens</i> (2) and <i>G.elegans</i> (1). Eight patients were male, their mean age was 46 years and four were younger than 21 years. The most frequent comorbidities were congenital heart disease (4; 40%) and the presence of intracardiac prosthetic material (5; 50%). IE occurred on 5 native valves and 5 prosthetic valve or material. Blood cultures were positive in 8/10 patients, within a mean incubation period of 18.07 hours. In the other two patients, a positive 16SPCR from valve or prosthetic material provided the diagnosis. Surgery for IE was performed in seven patients (70%) and in all cases positive <i>16S rRNA</i> PCR and sequencing from valve or prosthetic material was demonstrated. Valves and/or prosthetic removed material cultures were positive in four patients. Nine patients received ceftriaxone (4 in monotherapy and 5 in combination with other antibiotics). The mean length of treatment was 6 weeks and IE-associated mortality was 20% at one year follow-up.</p><p><strong>Conclusions: </strong><i>Abiotrophia</i> spp. or <i>Granulicatella</i> spp. IE were infrequent but not exceptional in our environment and particularly affected patients with congenital heart disease or prosthetic material. Blood cultures and molecular methods allowed the diagnosis. Most of them required surgery and the associated mortality, in spite of a mean age of 46 years, was high.</p>","PeriodicalId":73068,"journal":{"name":"Frontiers in bioscience (Elite edition)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33470139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Characterisation of the Faecal Microbiome of Foals from 0-5 Months of Age and Their Respective Mares across Five Geographic Locations.","authors":"Grace C O'Reilly,&nbsp;Devin B Holman,&nbsp;Katharine Muscat,&nbsp;Gary Muscatello,&nbsp;Mariano C Parra,&nbsp;Sarah J Meale,&nbsp;Alex V Chaves","doi":"10.31083/j.fbe1403022","DOIUrl":"https://doi.org/10.31083/j.fbe1403022","url":null,"abstract":"<p><strong>Background: </strong>A foal undergoes considerable growth and development from birth to weaning, progressing from a milk-based diet to complete herbivory. The symbiotic relationships between bacteria, archaea and fungi substantiate this energy demand by colonising the hindgut and remaining flexible throughout the diet transitions.</p><p><strong>Methods: </strong>A total of 70 faecal samples were collected from 14 mares and their foals across five studs in NSW as they aged from 0 to 5 months old. DNA was extracted from faecal samples and underwent amplification and sequencing of the 16S rRNA gene V4 hypervariable region of archaea and bacteria, and the fungal internal transcribed spacer-1 (ITS1) region. The fungal and bacterial community structure was assessed using Bray-Curtis dissimilarities, and the effect of age at sampling and location was determined using PERMANOVA.</p><p><strong>Results: </strong>Age at sampling had a substantial effect on the foal's archaeal and bacterial faecal microbiota (PERMANOVA: R2 = 0.16; <i>p</i> < 0.01), while the effect of geographical location was smaller but still significant (PERMANOVA: R2 = 0.07; <i>p</i> < 0.01). The overall abundance, diversity and richness of bacterial and archaeal populations increased (<i>p</i> < 0.01) as foals aged, most noticeably rising between foals 1 to 2 and 2 to 3 months of age. The 15 most relatively abundant fungal species were all environmental saprophytes, most strongly affected by geographical location (<i>p</i> < 0.01) rather than age at sampling. There was an effect of location on <i>Preussia Africana</i> (<i>p</i> = 0.02) and a location × age interaction for fungal species <i>Preussia persica</i> (<i>p</i> < 0.01), <i>Acremonium furcatum</i> (<i>p</i> = 0.04), and <i>Podospora pseudocomata</i> (<i>p</i> = 0.01). There was no effect of age, location, or location × age interaction on the relative abundance of the remaining fungal species.</p><p><strong>Conclusions: </strong>The faecal microbiome appeared to stabilise for most bacterial and archaeal genera by 2 to 3 months of age, resembling an adult mare. Bacterial genera isolated from faecal samples belonged mainly to the <i>Firmicutes</i> phylum. Age at sampling more strongly affected the archaeal and bacterial faecal microbiota than the effect of the geographical location where the horse was sampled. The lack of effect of location on microbe populations suggests that although environmental factors may influence population structure, there are distinct differences at each stage of foal maturation.</p>","PeriodicalId":73068,"journal":{"name":"Frontiers in bioscience (Elite edition)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33470135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Influence of Applications of Bio-Inputs Derived from Macroalgae and Bacteria on a <i>Phaseolus vulgaris</i> L. Crop.","authors":"Bruno Marques,&nbsp;Kiril Bahcevandziev,&nbsp;Paulo César de Melo,&nbsp;Alan T Critchley","doi":"10.31083/j.fbe1403021","DOIUrl":"https://doi.org/10.31083/j.fbe1403021","url":null,"abstract":"<p><strong>Background: </strong>The common bean (<i>Phaseolus vulgaris</i> L.), is one of the most relevant legumes worldwide, as a source of protein, fiber, carbohydrates, and biologically important minerals. In recent decades, bean production increased significantly, especially in developing countries, where the availability of animal protein is often in short supply. However, a large portion of this agricultural production has been achieved in an unsustainable manner, through the intensive use of non-renewable agrochemicals, which in both the short and long term negatively affect soil fertility. To address this problem, the use of sustainable and renewable bio-inputs derived from macroalgae, and microorganisms may be amongst solutions required. Extracts of seaweeds have been shown to be biodegradable and non-toxic both for treated plants and consumers. This study aimed to evaluate the influence of the application of three bio-inputs made from different organisms on a common bean variety (BRSMG Realce) by analysing plant physiology and productivity, pod morphology, nutritional and mineral characterization of the bean. The study also aimed to evaluate the length of BRSMG Realce crop life cycle and compare its nutritional value with other commonly consumed varieties.</p><p><strong>Methods: </strong>Six treatments were performed: T0 - Control; T1 - Calmar® (soil - 100 kg/ha); T2 - Profertil® (foliar - 0.5%(v/v)); T3 - Albit® (leaf - 0.02%(v/v)); T4 - Calmar® ((100 kg/ha) + Profertil® (0.5%(v/v)); T5 - Calmar® ((100 kg/ha) + Albit® (0.02% (v/v)).</p><p><strong>Results: </strong>The leaf chlorophyll index revealed significant increases for T2, T4 and T5, compared to control. In general, the treatments related to the pods morphology showed significant increases in the length/width ratio. In terms of productivity, significant increases were found with T1, T4 and T5. In the analysis of the nutritional value of dried beans there were significant increases in the contents of fiber in T1, protein in T4 and T5 and carbohydrates for T1, T2 and T3. For mineral composition, there were increases in the phosphorus content of T2, T4 and T5 beans. When the cooked beans were analysed, T4 and T1 produced a greater amount of ash and proteins, as compared to control.</p><p><strong>Conclusions: </strong>The applications of bio-inputs in the bean crop (<i>Phaseolus vulgaris</i> L.) exerted several positive and significant effects, mainly on the CCI, productivity, pod morphology as well as cooked bean nutritional values. It was verified that BRSMG Reakce has the potential to be included in the Portuguese diet.</p>","PeriodicalId":73068,"journal":{"name":"Frontiers in bioscience (Elite edition)","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2022-08-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"33479983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}