FEMS microbes最新文献_第5页

ynamics of Thioalkalivibrio species in a co-culture under selective pressure of ampicillin 硫代碱弧菌在氨苄西林选择性压力下的共培养动力学

FEMS microbes Pub Date : 2023-11-08 DOI: 10.1093/femsmc/xtad020

Anne-Catherine Ahn, J Merijn Schuurmans, Dimitry Sorokin, Gerard Muyzer

{"title":"ynamics of Thioalkalivibrio species in a co-culture under selective pressure of ampicillin","authors":"Anne-Catherine Ahn, J Merijn Schuurmans, Dimitry Sorokin, Gerard Muyzer","doi":"10.1093/femsmc/xtad020","DOIUrl":"https://doi.org/10.1093/femsmc/xtad020","url":null,"abstract":"Abstract Haloalkaliphilic chemolithoautotrophic sulfur-oxidizing bacteria belonging to the genus Thioalkalivibrio are highly abundant in microbial communities found in soda lakes and dominant in full-scale bioreactors removing sulfide from industrial waste gases. Despite certain soda lakes being remote and unaffected by anthropogenic activities, haloalkaliphilic microorganisms, including Thioalkalivibrio strains, possess various antibiotic resistance genes. In this study, we investigated the impact of the antibiotic ampicillin on a co-culture of two Thioalkalivibrio species, Tv. thiocyanoxidans ARh2T and Tv. versutus AL2T, both experimentally and through in silico analysis of antibiotic resistance. Cell growth dynamics were monitored over time at increasing ampicillin concentrations using rep- and qPCR. Within ten days after the addition of ampicillin, the co-culture transitioned from a Tv. thiocyanoxidans ARh2T-dominated to a stable Tv. versutus AL2T-dominated culture. This shift was attributed to Tv. versutus AL2T displaying a lower susceptibility to ampicillin, making it more competitive. These results emphasize the potential implications of antibiotic pressure on microbial communities, where a resistant species can outcompete a stable co-culture. This study presents the first evidence of such dynamics in haloalkaliphilic chemolithoautotrophs. By understanding the antibiotic resistance and the competitive dynamics of haloalkaliphilic bacteria like Thioalkalivibrio, we can gain insights into their behaviour and stress response.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"29 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135430049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enterococcus faecalis suppresses Staphylococcus aureus-induced NETosis and promotes bacterial survival in polymicrobial infections. 粪肠球菌抑制金黄色葡萄球菌诱导的NETosis，并在多种微生物感染中促进细菌存活。

FEMS microbes Pub Date : 2023-10-11 eCollection Date: 2023-01-01 DOI: 10.1093/femsmc/xtad019

Patrick Hsien-Neng Kao, Jun-Hong Ch'ng, Kelvin K L Chong, Claudia J Stocks, Siu Ling Wong, Kimberly A Kline

{"title":"Enterococcus faecalis suppresses Staphylococcus aureus-induced NETosis and promotes bacterial survival in polymicrobial infections.","authors":"Patrick Hsien-Neng Kao, Jun-Hong Ch'ng, Kelvin K L Chong, Claudia J Stocks, Siu Ling Wong, Kimberly A Kline","doi":"10.1093/femsmc/xtad019","DOIUrl":"https://doi.org/10.1093/femsmc/xtad019","url":null,"abstract":"Enterococcus faecalis is an opportunistic pathogen that is frequently co-isolated with other microbes in wound infections. While E. faecalis can subvert the host immune response and promote the survival of other microbes via interbacterial synergy, little is known about the impact of E. faecalis-mediated immune suppression on co-infecting microbes. We hypothesized that E. faecalis can attenuate neutrophil-mediated responses in mixed-species infection to promote survival of the co-infecting species. We found that neutrophils control E. faecalis infection via phagocytosis, ROS production, and degranulation of azurophilic granules, but it does not trigger neutrophil extracellular trap formation (NETosis). However, E. faecalis attenuates Staphylococcus aureus-induced NETosis in polymicrobial infection by interfering with citrullination of histone, suggesting E. faecalis can actively suppress NETosis in neutrophils. Residual S. aureus-induced NETs that remain during co-infection do not impact E. faecalis, further suggesting that E. faecalis possess mechanisms to evade or survive NET-associated killing mechanisms. E. faecalis-driven reduction of NETosis corresponds with higher S. aureus survival, indicating that this immunomodulating effect could be a risk factor in promoting the virulence polymicrobial infection. These findings highlight the complexity of the immune response to polymicrobial infections and suggest that attenuated pathogen-specific immune responses contribute to pathogenesis in the mammalian host.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"4 ","pages":"xtad019"},"PeriodicalIF":0.0,"publicationDate":"2023-10-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10608956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71415699","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The marula and elephant intoxication myth: assessing the biodiversity of fermenting yeasts associated with marula fruits (Sclerocarya birrea). 马鲁拉和大象中毒神话：评估与马鲁拉果实相关的发酵酵母的生物多样性。

FEMS microbes Pub Date : 2023-10-06 eCollection Date: 2023-01-01 DOI: 10.1093/femsmc/xtad018

Tawanda Proceed Makopa, Gorata Modikwe, Urska Vrhovsek, Cesare Lotti, José Paulo Sampaio, Nerve Zhou

{"title":"The marula and elephant intoxication myth: assessing the biodiversity of fermenting yeasts associated with marula fruits (Sclerocarya birrea).","authors":"Tawanda Proceed Makopa, Gorata Modikwe, Urska Vrhovsek, Cesare Lotti, José Paulo Sampaio, Nerve Zhou","doi":"10.1093/femsmc/xtad018","DOIUrl":"https://doi.org/10.1093/femsmc/xtad018","url":null,"abstract":"The inebriation of wild African elephants from eating the ripened and rotting fruit of the marula tree is a persistent myth in Southern Africa. However, the yeasts responsible for alcoholic fermentation to intoxicate the elephants remain poorly documented. In this study, we considered Botswana, a country with the world's largest population of wild elephants, and where the marula tree is indigenous, abundant and protected, to assess the occurrence and biodiversity of yeasts with a potential to ferment and subsequently inebriate the wild elephants. We collected marula fruits from over a stretch of 800 km in Botswana and isolated 106 yeast strains representing 24 yeast species. Over 93% of these isolates, typically known to ferment simple sugars and produce ethanol comprising of high ethanol producers belonging to Saccharomyces, Brettanomyces, and Pichia, and intermediate ethanol producers Wickerhamomyces, Zygotorulaspora, Candida, Hanseniaspora, and Kluyveromyces. Fermentation of marula juice revealed convincing fermentative and aromatic bouquet credentials to suggest the potential to influence foraging behaviour and inebriate elephants in nature. There is insufficient evidence to refute the aforementioned myth. This work serves as the first work towards understanding the biodiversity marula associated yeasts to debunk the myth or approve the facts.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"4 ","pages":"xtad018"},"PeriodicalIF":0.0,"publicationDate":"2023-10-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6a/53/xtad018.PMC10581541.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49685739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biotechnological potential of microbial bio-surfactants, their significance, and diverse applications. 微生物生物表面活性剂的生物技术潜力、意义和多种应用。

FEMS microbes Pub Date : 2023-08-10 eCollection Date: 2023-01-01 DOI: 10.1093/femsmc/xtad015

Renuka Kumari, Lairenjam Paikhomba Singha, Pratyoosh Shukla

{"title":"Biotechnological potential of microbial bio-surfactants, their significance, and diverse applications.","authors":"Renuka Kumari, Lairenjam Paikhomba Singha, Pratyoosh Shukla","doi":"10.1093/femsmc/xtad015","DOIUrl":"10.1093/femsmc/xtad015","url":null,"abstract":"Globally, there is a huge demand for chemically available surfactants in many industries, irrespective of their detrimental impact on the environment. Naturally occurring green sustainable substances have been proven to be the best alternative for reducing reliance on chemical surfactants and promoting long-lasting sustainable development. The most frequently utilized green active biosurfactants, which are made by bacteria, yeast, and fungi, are discussed in this review. These biosurfactants are commonly originated from contaminated sites, the marine ecosystem, and the natural environment, and it holds great potential for environmental sustainability. In this review, we described the importance of biosurfactants for the environment, including their biodegradability, low toxicity, environmental compatibility, and stability at a wide pH range. In this review, we have also described the various techniques that have been utilized to characterize and screen the generation of microbial biosurfactants. Also, we reviewed the potential of biosurfactants and its emerging applications in the foods, cosmetics, pharmaceuticals, and agricultural industries. In addition, we also discussed the ways to overcome problems with expensive costs such as low-cost substrate media formulation, gravitational techniques, and solvent-free foam fractionation for extraction that could be employed during biosurfactant production on a larger scale.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"4 ","pages":"xtad015"},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10442721/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10063587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LptD depletion disrupts morphological homeostasis and upregulates carbohydrate metabolism in Escherichia coli. LptD缺失破坏大肠杆菌的形态稳态并上调碳水化合物代谢。

FEMS microbes Pub Date : 2023-08-10 eCollection Date: 2023-01-01 DOI: 10.1093/femsmc/xtad013

Frida Svanberg Frisinger, Bimal Jana, Juan C Ortiz-Marquez, Tim van Opijnen, Stefano Donadio, Luca Guardabassi

{"title":"LptD depletion disrupts morphological homeostasis and upregulates carbohydrate metabolism in Escherichia coli.","authors":"Frida Svanberg Frisinger, Bimal Jana, Juan C Ortiz-Marquez, Tim van Opijnen, Stefano Donadio, Luca Guardabassi","doi":"10.1093/femsmc/xtad013","DOIUrl":"10.1093/femsmc/xtad013","url":null,"abstract":"In a previous in silico study, we identified an essential outer membrane protein (LptD) as an attractive target for development of novel antibiotics. Here, we characterized the effects of LptD depletion on Escherichia coli physiology and morphology. An E. coli CRISPR interference (CRISPRi) strain was constructed to allow control of lptD expression. Induction of the CRISPRi system led to ∼440-fold reduction of gene expression. Dose-dependent growth inhibition was observed, where strong knockdown effectively inhibited initial growth but partial knockdown exhibited maximum overall killing after 24 h. LptD depletion led to morphological changes where cells exhibited long, filamentous cell shapes and cytoplasmic accumulation of lipopolysaccharide (LPS). Transcriptional profiling by RNA-Seq showed that LptD knockdown led to upregulation of carbohydrate metabolism, especially in the colanic acid biosynthesis pathway. This pathway was further overexpressed in the presence of sublethal concentrations of colistin, an antibiotic targeting LPS, indicating a specific transcriptional response to this synergistic envelope damage. Additionally, exposure to colistin during LptD depletion resulted in downregulation of pathways related to motility and chemotaxis, two important virulence traits. Altogether, these results show that LptD depletion (i) affects E. coli survival, (ii) upregulates carbohydrate metabolism, and (iii) synergizes with the antimicrobial activity of colistin.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"4 ","pages":"xtad013"},"PeriodicalIF":0.0,"publicationDate":"2023-08-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10495129/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10232305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comprehensive mutation profiling from wastewater in southern Germany extends evidence of circulating SARS-CoV-2 diversity beyond mutations characteristic for Omicron. 从德国南部的废水中提取的全面突变图谱扩大了循环 SARS-CoV-2 多样性的证据范围，使其超越了 Omicron 的突变特征。

FEMS microbes Pub Date : 2023-03-03 eCollection Date: 2023-01-01 DOI: 10.1093/femsmc/xtad006

Shelesh Agrawal, Laura Orschler, Kira Zachmann, Susanne Lackner

{"title":"Comprehensive mutation profiling from wastewater in southern Germany extends evidence of circulating SARS-CoV-2 diversity beyond mutations characteristic for Omicron.","authors":"Shelesh Agrawal, Laura Orschler, Kira Zachmann, Susanne Lackner","doi":"10.1093/femsmc/xtad006","DOIUrl":"10.1093/femsmc/xtad006","url":null,"abstract":"Tracking SARS-CoV-2 variants in wastewater is primarily performed by detecting characteristic mutations of the variants. Unlike the Delta variant, the emergence of the Omicron variant and its sublineages as variants of concern has posed a challenge in using characteristic mutations for wastewater surveillance. In this study, we monitored the temporal and spatial variation of SARS-CoV-2 variants by including all the detected mutations and compared whether limiting the analyses to characteristic mutations for variants like Omicron impact the outcomes. We collected 24-hour composite samples from 15 wastewater treatment plants (WWTP) in Hesse and sequenced 164 wastewater samples with a targeted sequencing approach from September 2021 to March 2022. Our results show that comparing the number of all the mutations against the number of the characteristic mutations reveals a different outcome. A different temporal variation was observed for the ORF1a and S gene. As Omicron became dominant, we observed an increase in the overall number of mutations. Based on the characteristic mutations of the SARS-CoV-2 variants, a decreasing trend for the number of ORF1a and S gene mutations was noticed, though the number of known characteristic mutations in both genes is higher in Omicron than Delta.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"4 ","pages":"xtad006"},"PeriodicalIF":0.0,"publicationDate":"2023-03-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/f4/46/xtad006.PMC10117852.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9662431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Whole genomes of deep-sea sponge-associated bacteria exhibit high novel natural product potential. 深海海绵相关细菌的全基因组显示出很高的新型天然产物潜力。

FEMS microbes Pub Date : 2023-02-22 eCollection Date: 2023-01-01 DOI: 10.1093/femsmc/xtad005

Poppy J Hesketh-Best, Grant G January, Matthew J Koch, Philip J Warburton, Kerry L Howell, Mathew Upton

{"title":"Whole genomes of deep-sea sponge-associated bacteria exhibit high novel natural product potential.","authors":"Poppy J Hesketh-Best, Grant G January, Matthew J Koch, Philip J Warburton, Kerry L Howell, Mathew Upton","doi":"10.1093/femsmc/xtad005","DOIUrl":"10.1093/femsmc/xtad005","url":null,"abstract":"Global antimicrobial resistance is a health crisis that can change the face of modern medicine. Exploring diverse natural habitats for bacterially-derived novel antimicrobial compounds has historically been a successful strategy. The deep-sea presents an exciting opportunity for the cultivation of taxonomically novel organisms and exploring potentially chemically novel spaces. In this study, the draft genomes of 12 bacteria previously isolated from the deep-sea sponges Phenomena carpenteri and Hertwigia sp. are investigated for the diversity of specialized secondary metabolites. In addition, early data support the production of antibacterial inhibitory substances produced from a number of these strains, including activity against clinically relevant pathogens Acinetobacter baumannii, Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, and Staphylococcus aureus. Draft whole-genomes are presented of 12 deep-sea isolates, which include four potentially novel strains: Psychrobacter sp. PP-21, Streptomyces sp. DK15, Dietzia sp. PP-33, and Micrococcus sp. M4NT. Across the 12 draft genomes, 138 biosynthetic gene clusters were detected, of which over half displayed less than 50% similarity to known BGCs, suggesting that these genomes present an exciting opportunity to elucidate novel secondary metabolites. Exploring bacterial isolates belonging to the phylum Actinomycetota, Pseudomonadota, and Bacillota from understudied deep-sea sponges provided opportunities to search for new chemical diversity of interest to those working in antibiotic discovery.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"4 ","pages":"xtad005"},"PeriodicalIF":0.0,"publicationDate":"2023-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a8/1d/xtad005.PMC10117722.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10081532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Editorial: Thematic issue on bacterial-viral co-infections. 社论：细菌病毒共同感染专题。

FEMS microbes Pub Date : 2023-01-19 eCollection Date: 2023-01-01 DOI: 10.1093/femsmc/xtac031

Sam Manna, Daniel M Weinberger, Catherine Satzke

引用次数: 0

Direct wastewater extraction as a simple and effective method for SARS-CoV-2 surveillance and COVID-19 community-level monitoring. 直接废水提取是SARS-CoV-2监测和新冠肺炎社区级监测的一种简单有效的方法。

FEMS microbes Pub Date : 2023-01-12 eCollection Date: 2023-01-01 DOI: 10.1093/femsmc/xtad004

Megan E J Lott, William A Norfolk, Cody A Dailey, Amelia M Foley, Carolina Melendez-Declet, Megan J Robertson, Stephen L Rathbun, Erin K Lipp

{"title":"Direct wastewater extraction as a simple and effective method for SARS-CoV-2 surveillance and COVID-19 community-level monitoring.","authors":"Megan E J Lott, William A Norfolk, Cody A Dailey, Amelia M Foley, Carolina Melendez-Declet, Megan J Robertson, Stephen L Rathbun, Erin K Lipp","doi":"10.1093/femsmc/xtad004","DOIUrl":"10.1093/femsmc/xtad004","url":null,"abstract":"Wastewater surveillance has proven to be an effective tool to monitor the transmission and emergence of infectious agents at a community scale. Workflows for wastewater surveillance generally rely on concentration steps to increase the probability of detection of low-abundance targets, but preconcentration can substantially increase the time and cost of analyses while also introducing additional loss of target during processing. To address some of these issues, we conducted a longitudinal study implementing a simplified workflow for SARS-CoV-2 detection from wastewater, using a direct column-based extraction approach. Composite influent wastewater samples were collected weekly for 1 year between June 2020 and June 2021 in Athens-Clarke County, Georgia, USA. Bypassing any concentration step, low volumes (280 µl) of influent wastewater were extracted using a commercial kit, and immediately analyzed by RT-qPCR for the SARS-CoV-2 N1 and N2 gene targets. SARS-CoV-2 viral RNA was detected in 76% (193/254) of influent samples, and the recovery of the surrogate bovine coronavirus was 42% (IQR: 28%, 59%). N1 and N2 assay positivity, viral concentration, and flow-adjusted daily viral load correlated significantly with per-capita case reports of COVID-19 at the county-level (ρ = 0.69-0.82). To compensate for the method's high limit of detection (approximately 106-107 copies l-1 in wastewater), we extracted multiple small-volume replicates of each wastewater sample. With this approach, we detected as few as five cases of COVID-19 per 100 000 individuals. These results indicate that a direct-extraction-based workflow for SARS-CoV-2 wastewater surveillance can provide informative and actionable results.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"4 ","pages":"xtad004"},"PeriodicalIF":0.0,"publicationDate":"2023-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117872/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10036434","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Show us the data: global COVID-19 wastewater monitoring efforts, equity, and gaps. 向我们展示数据:全球COVID-19废水监测工作、公平性和差距。

FEMS microbes Pub Date : 2023-01-12 eCollection Date: 2023-01-01 DOI: 10.1093/femsmc/xtad003

Colleen C Naughton, Fernando A Roman, Ana Grace F Alvarado, Arianna Q Tariqi, Matthew A Deeming, Krystin F Kadonsky, Kyle Bibby, Aaron Bivins, Gertjan Medema, Warish Ahmed, Panagis Katsivelis, Vajra Allan, Ryan Sinclair, Joan B Rose

{"title":"Show us the data: global COVID-19 wastewater monitoring efforts, equity, and gaps.","authors":"Colleen C Naughton, Fernando A Roman, Ana Grace F Alvarado, Arianna Q Tariqi, Matthew A Deeming, Krystin F Kadonsky, Kyle Bibby, Aaron Bivins, Gertjan Medema, Warish Ahmed, Panagis Katsivelis, Vajra Allan, Ryan Sinclair, Joan B Rose","doi":"10.1093/femsmc/xtad003","DOIUrl":"10.1093/femsmc/xtad003","url":null,"abstract":"A year since the declaration of the global coronavirus disease 2019 (COVID-19) pandemic, there were over 110 million cases and 2.5 million deaths. Learning from methods to track community spread of other viruses such as poliovirus, environmental virologists and those in the wastewater-based epidemiology (WBE) field quickly adapted their existing methods to detect SARS-CoV-2 RNA in wastewater. Unlike COVID-19 case and mortality data, there was not a global dashboard to track wastewater monitoring of SARS-CoV-2 RNA worldwide. This study provides a 1-year review of the \"COVIDPoops19\" global dashboard of universities, sites, and countries monitoring SARS-CoV-2 RNA in wastewater. Methods to assemble the dashboard combined standard literature review, Google Form submissions, and daily, social media keyword searches. Over 200 universities, 1400 sites, and 55 countries with 59 dashboards monitored wastewater for SARS-CoV-2 RNA. However, monitoring was primarily in high-income countries (65%) with less access to this valuable tool in low- and middle-income countries (35%). Data were not widely shared publicly or accessible to researchers to further inform public health actions, perform meta-analysis, better coordinate, and determine equitable distribution of monitoring sites. For WBE to be used to its full potential during COVID-19 and beyond, show us the data.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"4 ","pages":"xtad003"},"PeriodicalIF":0.0,"publicationDate":"2023-01-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10117741/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10036431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 110