FEMS microbes最新文献

Silica hydrogels as a carbon-free solid media for the culture of diverse organisms. 二氧化硅水凝胶作为一种无碳固体培养基，用于多种生物的培养。

FEMS microbes Pub Date : 2024-12-28 eCollection Date: 2025-01-01 DOI: 10.1093/femsmc/xtae035

Druhi Vaid, Alisa Zubir, Alistair Hanak, Tanda Qi, Daniela Delneri, Lu Shin Wong

{"title":"Silica hydrogels as a carbon-free solid media for the culture of diverse organisms.","authors":"Druhi Vaid, Alisa Zubir, Alistair Hanak, Tanda Qi, Daniela Delneri, Lu Shin Wong","doi":"10.1093/femsmc/xtae035","DOIUrl":"10.1093/femsmc/xtae035","url":null,"abstract":"Bacteriological agar plates are commonly used to carry out experiments for the selective growth of microorganisms and the isolation of single-strain colonies. However, the presence of agar itself may be a confounding factor since it may serve as a source of carbon and energy. Moreover, there have been ongoing constraints on the production and sourcing of agar. These concerns have led to an interest in the development of agar substitutes. Silica hydrogels are entirely inorganic carbon-free polymeric materials that lack any source of micronutrients. Herein, a revised method for the preparation of silica hydrogels as a solid culture medium is reported. These gels can be formulated with a range of nutrient-rich or minimal media supplemented with various carbon sources, and can be manipulated in the same manner as agar gels. Their use for the culture and isolation of diverse microorganisms, including both Gram-positive and Gram-negative bacteria, yeast, and filamentous fungi is demonstrated. These silica hydrogels supplemented with either antibiotics or other molecules of interest can also be used for microbial selection experiments.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"6 ","pages":"xtae035"},"PeriodicalIF":0.0,"publicationDate":"2024-12-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11737323/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143017334","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Proteomic identification of a Toxoplasma gondii sporozoite-specific antigen using HDAC3 inhibitor-treated tachyzoites as surrogate. 以 HDAC3 抑制剂处理过的幼虫为替代物，从蛋白质组学角度鉴定弓形虫孢子虫特异性抗原。

FEMS microbes Pub Date : 2024-12-23 eCollection Date: 2025-01-01 DOI: 10.1093/femsmc/xtae034

David Warschkau, Sandra Klein, Ella Schadt, Joerg Doellinger, Gereon Schares, Frank Seeber

{"title":"Proteomic identification of a Toxoplasma gondii sporozoite-specific antigen using HDAC3 inhibitor-treated tachyzoites as surrogate.","authors":"David Warschkau, Sandra Klein, Ella Schadt, Joerg Doellinger, Gereon Schares, Frank Seeber","doi":"10.1093/femsmc/xtae034","DOIUrl":"10.1093/femsmc/xtae034","url":null,"abstract":"The apicomplexan parasite Toxoplasma gondii has a complex life cycle. Access to sexual stages and sporozoite-containing oocysts, essential for studying the parasite's environmental transmission, is limited and requires animal experiments with cats. Thus, alternatives and resource-efficient methods are needed. Several molecular factors and transcriptional switches responsible for differentiation have been identified in recent years. In tachyzoites, drug-induced inhibition of the histone deacetylase HDAC3, or genetic depletion of transcription factors regulating HDAC3, leads to the expression of genes that are specific to sexual stages and oocysts. Here, we applied this concept and showed that the commercially available HDAC3 inhibitor apicidin could be used to identify the hitherto unknown antigen of the sporozoite-specific monoclonal antibody G1/19 in tachyzoites. Using mass spectrometry of immunoprecipitated G1/19 target protein from apicidin-treated cultures, we identified it as SporoSAG. In addition, for the much less abundant sporozoite-specific protein LEA860, apicidin treatment was still sufficient to induce a detectable protein level in immunofluorescence microscopy. We also discuss further applications and the limitations of this approach. This allows to overcome issues with the paucity of material of sexual stages and oocysts from T. gondii to some extent without the need for cat-derived material.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"6 ","pages":"xtae034"},"PeriodicalIF":0.0,"publicationDate":"2024-12-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11719624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142973633","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluating the impact of redox potential on the growth capacity of anaerobic gut fungi. 评估氧化还原电位对厌氧肠道真菌生长能力的影响。

FEMS microbes Pub Date : 2024-11-05 eCollection Date: 2024-01-01 DOI: 10.1093/femsmc/xtae033

Emma E England, Carrie J Pratt, Mostafa S Elshahed, Noha H Youssef

{"title":"Evaluating the impact of redox potential on the growth capacity of anaerobic gut fungi.","authors":"Emma E England, Carrie J Pratt, Mostafa S Elshahed, Noha H Youssef","doi":"10.1093/femsmc/xtae033","DOIUrl":"10.1093/femsmc/xtae033","url":null,"abstract":"Anaerobic gut fungi (AGF, Neocallimastigomycota) inhabit the alimentary tract of herbivores. Although strict anaerobes, studies have suggested their capacity to retain viability after various durations of air exposure. It is currently unclear whether AGF can actively grow, and not merely survive, in redox potentials (Eh) higher than those encountered in the herbivorous gut. We evaluated the growth of two AGF strains (Orpinomyces joyonii and Testudinimyces gracilis) at various Eh levels, achieved by manipulating the concentrations of reductant (cysteine hydrochloride) in culture media. Both strains exhibited robust and sustainable growth at negative Eh (-50 mV or below). However, growth in the absence of cysteine hydrochloride (Eh value around +50 mV) was possible only for O. joyonii and only for one subcultivation. The capacity to grow at +50 mV was further confirmed in four additional taxa (Pecoramyces ruminatium, Anaeromyces mucronatus, Aklioshbmyces papillarum, and Piromyces communis), while two (Aestipascuomyces dupliciliberans and Capellomyces foraminis) failed to grow under these conditions. Our results establish the ability of AGF to grow at redox potential values higher than those encountered in their natural habitats. Such capability could contribute to efficient AGF dispersal and horizontal transmission between hosts, and could have important implications for industrial applications of AGF.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"5 ","pages":"xtae033"},"PeriodicalIF":0.0,"publicationDate":"2024-11-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11575491/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142677956","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Contact with young children is a major risk factor for pneumococcal colonization in older adults. 与幼儿接触是老年人肺炎球菌定植的主要风险因素。

FEMS microbes Pub Date : 2024-10-14 eCollection Date: 2024-01-01 DOI: 10.1093/femsmc/xtae032

Anne L Wyllie, Devyn Yolda-Carr, Maikel S Hislop, Sidiya Mbodj, Loren Wurst, Pari Waghela, Ronika Alexander-Parrish, Lindsay R Grant, Adriano Arguedas, Bradford D Gessner, Daniel M Weinberger

{"title":"Contact with young children is a major risk factor for pneumococcal colonization in older adults.","authors":"Anne L Wyllie, Devyn Yolda-Carr, Maikel S Hislop, Sidiya Mbodj, Loren Wurst, Pari Waghela, Ronika Alexander-Parrish, Lindsay R Grant, Adriano Arguedas, Bradford D Gessner, Daniel M Weinberger","doi":"10.1093/femsmc/xtae032","DOIUrl":"10.1093/femsmc/xtae032","url":null,"abstract":"Important questions remain about the sources of transmission of pneumococcus to older adults in the community. This is critical for understanding the potential effects of using pneumococcal conjugate vaccines (PCVs) in children and older adults. For non-institutionalized individuals, we hypothesized that the most likely source of adult-to-adult transmission is within the household. We designed a longitudinal study to sample adults ≥60 years of age living in the same household (New Haven, CT, USA), without younger residents in the household. Saliva samples and social and health questionnaires were obtained every 2 weeks for a period of 10 weeks. DNA extracted from culture-enriched saliva was tested using qPCR for pneumococcus genes piaB, lytA, and serotype. Across two study seasons (November 2020-August 2021, November 2021-September 2022), 121 individuals from 61 households completed all six visits; 62 individuals were enrolled in both seasons. Overall, 52/1088 (4.8%) samples tested positive for pneumococcus, with 27/121 (22.3%) individuals colonized at least once. Several individuals were colonized at multiple time points; two individuals were colonized at 5/6 time points and two at all six. In 5 instances, both household members were carriers in the same season, though not necessarily at the same time. Pneumococcal carriage was substantially higher among individuals who had contact with children (10.0% vs. 1.6%). Contact with young children was the most important factor that influenced pneumococcal acquisition rates. While there were several instances where both adult household members were colonized at the same time or at sequential visits, these individuals typically had contact with children. As such, PCV immunization can directly protect older adults who have contact with children.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"5 ","pages":"xtae032"},"PeriodicalIF":0.0,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11536760/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142585124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Trivalent immunization with metal-binding proteins confers protection against enterococci in a mouse infection model. 在小鼠感染模型中，金属结合蛋白的三价免疫可提供对肠球菌的保护。

FEMS microbes Pub Date : 2024-10-03 eCollection Date: 2024-01-01 DOI: 10.1093/femsmc/xtae031

Ling Ning Lam, Angie Sedra, Jessica Kajfasz, Aym Berges, Irene S Saengpet, Grace Adams, Jeffery Fairman, José A Lemos

{"title":"Trivalent immunization with metal-binding proteins confers protection against enterococci in a mouse infection model.","authors":"Ling Ning Lam, Angie Sedra, Jessica Kajfasz, Aym Berges, Irene S Saengpet, Grace Adams, Jeffery Fairman, José A Lemos","doi":"10.1093/femsmc/xtae031","DOIUrl":"https://doi.org/10.1093/femsmc/xtae031","url":null,"abstract":"Enterococcus faecalis is ranked among the top five bacterial pathogens responsible for catheter-associated urinary tract infections, wound infections, secondary root canal infections, and infective endocarditis. Previously, we showed that inactivation of either the manganese- and iron-binding (EfaA) or zinc-binding (AdcA and AdcAII) lipoproteins significantly reduced E. faecalis virulence. Here, we explored whether immunization using a multi-valent approach induces protective immunity against systemic enterococcal infections. We found that multi-antigen antisera raised against EfaA, AdcA, and AdcAII displayed similar capacities to initiate neutrophil-mediated opsonization, like their single-antigen counterparts. Further, these antigen-specific antibodies worked synergistically with calprotectin, a divalent host metal chelator, to inhibit the growth of E. faecalis in laboratory media as well as in human sera. Using the Galleria mellonella invertebrate model and mouse peritonitis model, we showed that passive immunization with multi-antigen antisera conferred robust protection against E. faecalis infection, while the protective effects of single antigen antisera were negligible in G. mellonella, and negligible-to-moderate in the mouse model. Lastly, active immunization with the 3-antigen (trivalent) cocktail significantly protected mice against either lethal or non-lethal E. faecalis infections, with this protection appearing to be far-reaching based on immunization results obtained with contemporary strains of E. faecalis and closely related Enterococcus faecium.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"5 ","pages":"xtae031"},"PeriodicalIF":0.0,"publicationDate":"2024-10-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11549557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634036","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Arginine impacts aggregation, biofilm formation, and antibiotic susceptibility in Enterococcus faecalis. 精氨酸会影响粪肠球菌的聚集、生物膜形成和抗生素敏感性。

FEMS microbes Pub Date : 2024-09-25 eCollection Date: 2024-01-01 DOI: 10.1093/femsmc/xtae030

Alex P Snell, Dawn A Manias, Reham R Elbehery, Gary M Dunny, Julia L E Willett

{"title":"Arginine impacts aggregation, biofilm formation, and antibiotic susceptibility in Enterococcus faecalis.","authors":"Alex P Snell, Dawn A Manias, Reham R Elbehery, Gary M Dunny, Julia L E Willett","doi":"10.1093/femsmc/xtae030","DOIUrl":"10.1093/femsmc/xtae030","url":null,"abstract":"Enterococcus faecalis is a commensal bacterium in the gastrointestinal (GI) tract of humans and other organisms. E. faecalis also causes infections in root canals, wounds, the urinary tract, and on heart valves. E. faecalis metabolizes arginine through the arginine deiminase pathway, which converts arginine to ornithine and releases ATP, ammonia, and CO2. E. faecalis arginine metabolism also affects virulence of other pathogens during co-culture. E. faecalis may encounter elevated levels of arginine in the GI tract or the oral cavity, where arginine is used as a dental therapeutic. Little is known about how E. faecalis responds to growth in arginine in the absence of other bacteria. To address this, we used RNAseq and additional assays to measure growth, gene expression, and biofilm formation in E. faecalis OG1RF grown in arginine. We demonstrate that arginine decreases E. faecalis biofilm production and causes widespread differential expression of genes related to metabolism, quorum sensing, and polysaccharide synthesis. Growth in arginine also increases aggregation of E. faecalis and promotes decreased susceptibility to the antibiotics ampicillin and ceftriaxone. This work provides a platform for understanding how the presence of arginine in biological niches affects E. faecalis physiology and virulence of surrounding microbes.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"5 ","pages":"xtae030"},"PeriodicalIF":0.0,"publicationDate":"2024-09-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11549559/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pandemic storytelling and student engagement: how students imagined pandemics before COVID-19 pandemic. 讲大流行病故事与学生参与：在 COVID-19 大流行之前学生如何想象大流行病。

FEMS microbes Pub Date : 2024-09-17 eCollection Date: 2024-01-01 DOI: 10.1093/femsmc/xtae029

Moamen M Elmassry

{"title":"Pandemic storytelling and student engagement: how students imagined pandemics before COVID-19 pandemic.","authors":"Moamen M Elmassry","doi":"10.1093/femsmc/xtae029","DOIUrl":"https://doi.org/10.1093/femsmc/xtae029","url":null,"abstract":"Student engagement is one of the critical issues in science classes. This commentary explores the value of storytelling in microbiology education and student engagement. It is a result of an undergraduate exercise, where students were asked to draft short stories on pandemics before the COVID-19 pandemic (i.e. conducted in 2016-2019). The analysis of student writings (total of 244 short stories) aimed to understand the students' perception of pandemics, the diseases they chose to write about, and their level of knowledge of pandemics. Interestingly, 56.6% of students chose viruses to be the cause of their pandemics, 35.7% chose bacteria, 4.5% chose parasites, and a single student chose a fungus. Respiratory mode of transmission was the top pick in the stories (30%), followed by fecal-oral route, sexually transmitted, and skin-to-skin contact. Therefore, their choice of respiratory diseases as the focus of their short story may suggest their limited understanding of pandemics beyond such diseases. The varying levels of detail in the stories suggested that storytelling could be insightful in identifying knowledge gaps and engaging students. Two exciting questions remain: (1) For the students who wrote those short stories before the COVID-19 pandemic, how did they feel when it hit? (2) If the students are asked again to write a pandemic short story, what would it look like nowadays? A deeper understanding of epidemiology and the social impact of pandemics is crucial for developing effective pandemic preparedness and response plans, and undergraduate courses can play a vital role in this endeavor. Overall, this commentary highlights the value of storytelling in engaging students to assess their knowledge of specific subject matters.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"5 ","pages":"xtae029"},"PeriodicalIF":0.0,"publicationDate":"2024-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11549555/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634030","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring near-infrared spectroscopy and hyperspectral imaging as novel characterization methods for anaerobic gut fungi. 探索近红外光谱和高光谱成像作为厌氧肠道真菌的新型表征方法。

FEMS microbes Pub Date : 2024-09-10 eCollection Date: 2024-01-01 DOI: 10.1093/femsmc/xtae025

Markus Neurauter, Julia M Vinzelj, Sophia F A Strobl, Christoph Kappacher, Tobias Schlappack, Jovan Badzoka, Matthias Rainer, Christian W Huck, Sabine M Podmirseg

{"title":"Exploring near-infrared spectroscopy and hyperspectral imaging as novel characterization methods for anaerobic gut fungi.","authors":"Markus Neurauter, Julia M Vinzelj, Sophia F A Strobl, Christoph Kappacher, Tobias Schlappack, Jovan Badzoka, Matthias Rainer, Christian W Huck, Sabine M Podmirseg","doi":"10.1093/femsmc/xtae025","DOIUrl":"10.1093/femsmc/xtae025","url":null,"abstract":"Neocallimastigomycota are a phylum of anaerobic gut fungi (AGF) that inhabit the gastrointestinal tract of herbivores and play a pivotal role in plant matter degradation. Their identification and characterization with marker gene regions has long been hampered due to the high inter- and intraspecies length variability in the commonly used fungal marker gene region internal transcribed spacer (ITS). While recent research has improved methodology (i.e. switch to LSU D2 as marker region), molecular methods will always introduce bias through nucleic acid extraction or PCR amplification. Here, near-infrared spectroscopy (NIRS) and hyperspectral imaging (HSI) are introduced as two nucleic acid sequence-independent tools for the characterization and identification of AGF strains. We present a proof-of-concept for both, achieving an independent prediction accuracy of above 95% for models based on discriminant analysis trained with samples of three different genera. We further demonstrated the robustness of the NIRS model by testing it on cultures of different growth times. Overall, NIRS provides a simple, reliable, and nondestructive approach for AGF classification, independent of molecular approaches. The HSI method provides further advantages by requiring less biomass and adding spatial information, a valuable feature if this method is extended to mixed cultures or environmental samples in the future.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"5 ","pages":"xtae025"},"PeriodicalIF":0.0,"publicationDate":"2024-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11412074/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142302487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enterococcal-host interactions in the gastrointestinal tract and beyond. 肠道内外肠球菌与宿主的相互作用

FEMS microbes Pub Date : 2024-09-09 eCollection Date: 2024-01-01 DOI: 10.1093/femsmc/xtae027

Wiam Abdalla Mo Madani, Yusibeska Ramos, Juan R Cubillos-Ruiz, Diana K Morales

{"title":"Enterococcal-host interactions in the gastrointestinal tract and beyond.","authors":"Wiam Abdalla Mo Madani, Yusibeska Ramos, Juan R Cubillos-Ruiz, Diana K Morales","doi":"10.1093/femsmc/xtae027","DOIUrl":"10.1093/femsmc/xtae027","url":null,"abstract":"The gastrointestinal tract (GIT) is typically considered the natural niche of enterococci. However, these bacteria also inhabit extraintestinal tissues, where they can disrupt organ physiology and cause life-threatening infections. Here, we discuss how enterococci, primarily Enterococcus faecalis, interact with the intestine and other host anatomical locations such as the oral cavity, heart, liver, kidney, and vaginal tract. The metabolic flexibility of these bacteria allows them to quickly adapt to new environments, promoting their persistence in diverse tissues. In transitioning from commensals to pathogens, enterococci must overcome harsh conditions such as nutrient competition, exposure to antimicrobials, and immune pressure. Therefore, enterococci have evolved multiple mechanisms to adhere, colonize, persist, and endure these challenges in the host. This review provides a comprehensive overview of how enterococci interact with diverse host cells and tissues across multiple organ systems, highlighting the key molecular pathways that mediate enterococcal adaptation, persistence, and pathogenic behavior.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"5 ","pages":"xtae027"},"PeriodicalIF":0.0,"publicationDate":"2024-09-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11466040/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142402214","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterization of the metabolism of the yeast Yarrowia lipolytica growing as a biofilm. 以生物膜形式生长的脂溶性亚罗酵母的新陈代谢特征。

FEMS microbes Pub Date : 2024-08-24 eCollection Date: 2024-01-01 DOI: 10.1093/femsmc/xtae026

Akarawit Jenjitwanich, Hans Marx, Michael Sauer

{"title":"Characterization of the metabolism of the yeast Yarrowia lipolytica growing as a biofilm.","authors":"Akarawit Jenjitwanich, Hans Marx, Michael Sauer","doi":"10.1093/femsmc/xtae026","DOIUrl":"https://doi.org/10.1093/femsmc/xtae026","url":null,"abstract":"Yarrowia lipolytica is a well-characterized yeast with remarkable metabolic adaptability. It is capable of producing various products from different carbon sources and easily switching between planktonic and biofilm states. A biofilm represents a natural means of cell immobilization that could support continuous cultivation and production processes, such as perfusion cultivation. However, the metabolic activities of Y. lipolytica in biofilms have not yet been studied in detail. Therefore, this study aimed to compare the metabolic activities of Y. lipolytica in biofilm and planktonic states. Conventionally, a stirred tank bioreactor was used to cultivate Y. lipolytica in a planktonic state. On the other hand, a trickle bed bioreactor system was used for biofilm cultivation. The low pH at 3 was maintained to favor polyol production. The accumulation of citric acid was observed over time only in the biofilm state, which significantly differed from the planktonic state. Although the biofilm cultivation process has lower productivity, it has been observed that the production rate remains constant and the total product yield is comparable to the planktonic state when supplied with 42% oxygen-enriched air. This finding indicates that the biofilm state has the potential for continuous bioprocessing applications and is possibly a feasible option.","PeriodicalId":73024,"journal":{"name":"FEMS microbes","volume":"5 ","pages":"xtae026"},"PeriodicalIF":0.0,"publicationDate":"2024-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11552517/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142634115","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0