ynamics of Thioalkalivibrio species in a co-culture under selective pressure of ampicillin

Anne-Catherine Ahn, J Merijn Schuurmans, Dimitry Sorokin, Gerard Muyzer
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引用次数: 0

Abstract

Abstract Haloalkaliphilic chemolithoautotrophic sulfur-oxidizing bacteria belonging to the genus Thioalkalivibrio are highly abundant in microbial communities found in soda lakes and dominant in full-scale bioreactors removing sulfide from industrial waste gases. Despite certain soda lakes being remote and unaffected by anthropogenic activities, haloalkaliphilic microorganisms, including Thioalkalivibrio strains, possess various antibiotic resistance genes. In this study, we investigated the impact of the antibiotic ampicillin on a co-culture of two Thioalkalivibrio species, Tv. thiocyanoxidans ARh2T and Tv. versutus AL2T, both experimentally and through in silico analysis of antibiotic resistance. Cell growth dynamics were monitored over time at increasing ampicillin concentrations using rep- and qPCR. Within ten days after the addition of ampicillin, the co-culture transitioned from a Tv. thiocyanoxidans ARh2T-dominated to a stable Tv. versutus AL2T-dominated culture. This shift was attributed to Tv. versutus AL2T displaying a lower susceptibility to ampicillin, making it more competitive. These results emphasize the potential implications of antibiotic pressure on microbial communities, where a resistant species can outcompete a stable co-culture. This study presents the first evidence of such dynamics in haloalkaliphilic chemolithoautotrophs. By understanding the antibiotic resistance and the competitive dynamics of haloalkaliphilic bacteria like Thioalkalivibrio, we can gain insights into their behaviour and stress response.
硫代碱弧菌在氨苄西林选择性压力下的共培养动力学
嗜盐嗜碱化石自养硫氧化菌属硫酸弧菌在碱湖微生物群落中数量丰富,在工业废气硫化物脱除的全尺寸生物反应器中占主导地位。尽管某些苏打湖地处偏远,不受人为活动的影响,但包括硫代碱弧菌菌株在内的嗜盐碱微生物具有各种抗生素抗性基因。在这项研究中,我们研究了抗生素氨苄西林对两种硫酸弧菌Tv共培养的影响。硫氰氧化物ARh2T和Tv。versutus AL2T,通过实验和计算机分析抗生素耐药性。在增加氨苄西林浓度时,使用rep-和qPCR监测细胞生长动态。在加入氨苄西林后10天内,共培养从Tv转变为Tv。硫氰化物以arh2t为主,趋于稳定。al2t主导的文化。这种转变归因于电视。相反,AL2T对氨苄西林的敏感性较低,使其更具竞争力。这些结果强调了抗生素压力对微生物群落的潜在影响,其中耐药物种可以胜过稳定的共培养。这项研究提出了在嗜盐嗜碱化石自养生物中这种动态的第一个证据。通过了解抗生素耐药性和硫代碱弧菌等嗜盐菌的竞争动态,我们可以深入了解它们的行为和应激反应。
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来源期刊
CiteScore
3.30
自引率
0.00%
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0
审稿时长
15 weeks
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