Addiction Biology最新文献

{"title":"Associations Between Stress and Hair Cortisol and Their Relationship to Alcohol Use Among Adolescents and Young Adults: An Epidemiological Cohort Study","authors":"Lena Plettenberg,&nbsp;Anja Kräplin,&nbsp;Catharina Voss,&nbsp;Katja Beesdo-Baum,&nbsp;Hanna Kische","doi":"10.1111/adb.70018","DOIUrl":"https://doi.org/10.1111/adb.70018","url":null,"abstract":"<p>The relationship between stress, hair cortisol and alcohol consumption has mostly been investigated among clinical and adult study samples, with inconsistent findings. The present study aimed to examine cross-sectional and longitudinal associations between chronic stress, hair cortisol and average past-year alcohol consumption within a population-based sample of adolescents and young adults. At baseline of the epidemiological cohort study, <i>N</i> = 1180 individuals aged 14–21 from Dresden, Germany, were assessed (11/2015–12/2016). A maximum <i>N</i> = 1055 were analysed in cross-sectional analyses and a maximum <i>N</i> = 722 in longitudinal analyses (1-year follow-up). Multivariate linear regression analyses were conducted to reveal cross-sectional associations between perceived chronic stress, hair cortisol concentration and average past-year alcohol consumption in males and females. Longitudinally, weighted linear regression models examined relationships between (a) perceived chronic stress at baseline and altered hair cortisol concentration over 1 year, (b) perceived chronic stress/hair cortisol concentration at baseline and altered average alcohol consumption over 1 year and (c) average past-year alcohol consumption at baseline and altered stress/hair cortisol concentration over 1 year. Cross-sectionally, no significant relationships were found between stress, hair cortisol and average past-year alcohol consumption at baseline. In females, higher baseline perceived chronic stress was associated with an increase in hair cortisol concentration over 1 year, whereas no relationship was found in the cross-sectional analysis between baseline perceived chronic stress and baseline past-year average alcohol consumption. When using hair cortisol as a biomarker for stress perception, the focus of future research should be on potential time lags between perceived chronic stress and hair cortisol increase.</p>","PeriodicalId":7289,"journal":{"name":"Addiction Biology","volume":"30 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/adb.70018","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143396998","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Alcohol induces concentration-dependent transcriptomic changes in oligodendrocytes","authors":"Sam A. Bazzi,&nbsp;Cole Maguire,&nbsp;R. Dayne Mayfield,&nbsp;Esther Melamed","doi":"10.1111/adb.70012","DOIUrl":"https://doi.org/10.1111/adb.70012","url":null,"abstract":"<p>Oligodendrocytes are a key cell type within the central nervous system (CNS) that generates the myelin sheath covering axons, enabling fast propagation of neuronal signals. Alcohol consumption is known to affect oligodendrocytes and white matter in the CNS. However, most studies have focused on foetal alcohol spectrum disorder and severe alcohol use disorder. Additionally, the impact of alcohol dosage on oligodendrocytes has not been previously investigated. In this study, we evaluated transcriptomic changes in C57BL6/J cultured mature oligodendrocytes following exposure to moderate and high concentrations of alcohol. We found that high concentrations of alcohol elicited gene expression changes across a wide range of biological pathways, including myelination, protein translation, integrin signalling, cell cycle regulation and inflammation. Further, our results demonstrate that transcriptomic changes are indeed dependent on alcohol concentration, with moderate and high concentrations of alcohol provoking distinct gene expression profiles. In conclusion, our study demonstrates that alcohol-induced transcriptomic changes in oligodendrocytes are concentration-dependent and may have critical downstream impacts on myelin production. Targeting alcohol-induced changes in cell cycle regulation, integrin signalling, inflammation or protein translation regulation may uncover mechanisms for modulating myelin production or inhibition. Furthermore, gaining a deeper understanding of alcohol's effects on oligodendrocyte demyelination and remyelination could help uncover therapeutic pathways that can be utilized independently of alcohol to aid in remyelinating drug design.</p>","PeriodicalId":7289,"journal":{"name":"Addiction Biology","volume":"30 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/adb.70012","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced Alcohol Consumption Following Ablation of Cholinergic Interneurons in the Nucleus Accumbens of Wistar Rats","authors":"Anna Loftén,&nbsp;Davide Cadeddu,&nbsp;Klara Danielsson,&nbsp;Rosita Stomberg,&nbsp;Louise Adermark,&nbsp;Bo Söderpalm,&nbsp;Mia Ericson","doi":"10.1111/adb.70022","DOIUrl":"https://doi.org/10.1111/adb.70022","url":null,"abstract":"<p>Alcohol use disorder is a severe mental health condition causing medical consequences and preterm death. Alcohol activates the mesolimbic dopamine system leading to an increase of extracellular dopamine (DA) in the nucleus accumbens, an event that is associated with the reinforcing effects of alcohol. Cholinergic interneurons (CIN) are important modulators of accumbal DA signalling, and depletion of accumbal CIN attenuates the alcohol-induced increase in extracellular DA. The aim of this study was to explore the functional role of accumbal CIN in alcohol-related behaviour. To this end, ablation of CIN was induced by local administration of anticholine acetyltransferase-saporin bilaterally into the nucleus accumbens of male Wistar rats. Alcohol consumption in ablated and sham-treated rats was studied using a two-bottle-choice intermittent alcohol consumption paradigm. Rats with depleted CIN consumed significantly less alcohol than sham-treated controls. No differences in sucrose preference, motor activity, water intake or weight gain were noted between treatment groups, suggesting that the ablation selectively affected alcohol-related behaviour. In conclusion, this study further supports a role for accumbal CIN in regulating alcohol-consummatory behaviour.</p>","PeriodicalId":7289,"journal":{"name":"Addiction Biology","volume":"30 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-02-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/adb.70022","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143389251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"iTRAQ proteomic analysis of the anterior insula in morphine-induced conditioned place preference rats with high-frequency deep brain stimulation intervention","authors":"Haigang Chang,&nbsp;Yaxiao Wang,&nbsp;Lei Hui,&nbsp;Yuling Diao,&nbsp;Pengju Ma,&nbsp;Xiangsheng Li,&nbsp;Feng Wang","doi":"10.1111/adb.70014","DOIUrl":"10.1111/adb.70014","url":null,"abstract":"<p>Morphine dependence or addiction is a serious global public health and social problem, and traditional treatments are very limited. Deep brain stimulation (DBS) has emerged as a new potential treatment for drug addiction. Repeated use of morphine leads to neuroadaptive and molecular changes in the addiction-related brain regions. We have previously performed isobaric tags for relative and absolute quantitation (iTRAQ) labelling coupled with 2D-LC MS/MS in anterior insular samples from rats treated with saline control, morphine or morphine plus DBS, and the identified expression of eight proteins are altered by morphine and reversed by high-frequency DBS (HF-DBS). In this study, we analysed the proteomic data in more details. A total of 5575 proteins were identified. Relative to the saline group, the morphine group showed 14 down-regulated and three up-regulated proteins. There were 118 proteins increased and 87 proteins decreased between DBS implanted animals and morphine group. Several differentially expressed proteins were verified with parallel reaction monitoring (PRM) assay. Based on Gene Ontology enrichment an KEGG pathway analyses, the majority of these differentially expressed proteins (DEPs) were involved in protein metabolic process, G-protein coupled receptor signalling pathway, calcium-mediated signalling, neurotransmitter transport, dopaminergic synapse and mTOR signalling pathway. These data offer a comprehensive understanding of the proteomic changes associated with morphine addiction and DBS therapy in addicted animal models, which is important for the development of DBS interventions for drug addiction.</p>","PeriodicalId":7289,"journal":{"name":"Addiction Biology","volume":"30 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11747870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143014336","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Vaping—An Emerging Threat to Youngsters of Pakistan","authors":"Nazish Jaffar,&nbsp;Hafiza Tooba Siddiqui,&nbsp;Huda Amin,&nbsp;Md Ariful Haque","doi":"10.1111/adb.70017","DOIUrl":"10.1111/adb.70017","url":null,"abstract":"&lt;p&gt;We would like to draw your attention to a critical prevailing issue regarding the usage of e-cigarettes and their ill effects on health. E-cigarettes have been marketed as a safer alternative to traditional tobacco products, but research has shown that they still pose significant health risks. Studies have revealed that the chemical compounds in e-cigarettes can cause damage to the lungs, heart and nervous system [&lt;span&gt;1&lt;/span&gt;].&lt;/p&gt;&lt;p&gt;With the boom in vaping culture, many people believe that e-cigarettes are a healthier alternative to traditional cigarettes. However, research studies have shown otherwise. Studies have shown that e-cigarettes can be harmful to human health, particularly among teenagers whose brains and respiratory systems are still developing [&lt;span&gt;2&lt;/span&gt;]. An interesting study was published in your esteemed journal, &lt;i&gt;Addiction Biology&lt;/i&gt;, on the neural performance of abstinent smokers. It aimed to find out the alterations in the brain networks. Before and after using e-cigarettes, this experiment revealed that the impact of e-cigarettes could be similar to neural activity caused by traditional cigarettes and other forms, which may lead to addiction [&lt;span&gt;3&lt;/span&gt;].&lt;/p&gt;&lt;p&gt;To begin with, it is essential to understand that e-cigarettes contain nicotine, which can lead to addiction and dependency similar to regular tobacco products. Studies suggest that vaping can expose users to higher levels of nicotine than traditional smoking, as well as other harmful chemicals such as formaldehyde and acetaldehyde. These chemicals are known carcinogens and could cause long-term harm to the respiratory system (Figure 1). This shows the average exposure to substances used in e-cigarettes. To calculate the chemical exposure from tobacco-related items, a study was performed where the toxicological threshold for margins of exposure (MOE) for each chemical was determined as MOE &lt; 10 as ‘high risk’. At the same time, MOE &lt; 100 was judged as ‘risk’. More than 100 were acceptable. In this experiment, nicotine showed (a margin of exposure) MOE &lt; 1, making it fall in the high-risk category [&lt;span&gt;3, 4&lt;/span&gt;]. Therefore, increasing public awareness about the potential risks of using e-cigarettes is crucial.&lt;/p&gt;&lt;p&gt;Moreover, there is increasing evidence linking e-cigarette use with cardiovascular risk factors such as high blood pressure and impaired heart function. Nicotine plays an integral role in these risks by constricting arteries and narrowing blood vessels, thereby reducing oxygen supply throughout the body and leading to damage over time [&lt;span&gt;5&lt;/span&gt;]. As the trends in everything change, social media is a new platform where businesses market their products in the marketing industry. One of its ways is how influencers are positively marketing E-cigarettes with no age restrictions and no trigger warning related to their adverse effects. In countries like Asia and the US, most of the followers are youngsters aged 13–17, which wil","PeriodicalId":7289,"journal":{"name":"Addiction Biology","volume":"30 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11750584/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143014429","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeting VMPFC-amygdala circuit with TMS in substance use disorder: A mechanistic framework","authors":"Ghazaleh Soleimani,&nbsp;Christine A. Conelea,&nbsp;Rayus Kuplicki,&nbsp;Alexander Opitz,&nbsp;Kelvin O. Lim,&nbsp;Martin P. Paulus,&nbsp;Hamed Ekhtiari","doi":"10.1111/adb.70011","DOIUrl":"10.1111/adb.70011","url":null,"abstract":"<p>The ventromedial prefrontal cortex (VMPFC), located along the medial aspect of the frontal area, plays a critical role in regulating arousal/emotions. Its intricate connections with subcortical structures, including the striatum and amygdala, highlight the VMPFC's importance in the neurocircuitry of addiction. Due to these features, the VMPFC is considered a promising target for transcranial magnetic stimulation (TMS) in substance use disorders (SUD). By the end of 2023, all 21 studies targeting VMPFC for SUD used anatomical landmarks (e.g., Fp1/Fp2 in the EEG system) to define coil location with a fixed orientation. Nevertheless, one-size-fits-all TMS over VMPFC has yielded variable outcomes. Here, we suggested a pipeline based on a tailored TMS targeting framework aimed at optimally modulating the VMPFC-amygdala circuit on an individual basis. We collected MRI data from 60 participants with methamphetamine use disorders (MUDs). We examined the variability in TMS target location based on task-based functional connectivity between VMPFC and amygdala using psychophysiological interaction (PPI) analysis. Electric fields (EF) were calculated for fixed vs. optimized location (Fp1/Fp2 vs. individualized maximal PPI), orientation (AF7/AF8 vs. optimized algorithm) and intensity (constant vs. adjusted) to maximize target engagement. In our pipeline, the left medial amygdala, identified as the brain region with the highest (0.31 ± 0.29) fMRI drug cue reactivity, was selected as the subcortical seed region. The voxel with the most positive amygdala-VMPFC PPI connectivity in each participant was considered the individualized TMS target (MNI-coordinates: [12.6, 64.23, −0.8] ± [13.64, 3.50, 11.01]). This individualized VMPFC-amygdala connectivity significantly correlated with VAS craving after cue exposure (R = 0.27, p = 0.03). Coil orientation was optimized to increase EF strength over the targeted circuit (0.99 ± 0.21 V/m vs. the fixed approach: Fp1: 0.56 ± 0.22 and Fp2: 0.78 ± 0.25 V/m) and TMS intensity was harmonized across the population. This study highlights the potential of an individualized VMPFC targeting framework to enhance treatment outcomes for addiction, specifically modulating the personalized VMPFC-amygdala circuit.</p>","PeriodicalId":7289,"journal":{"name":"Addiction Biology","volume":"30 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11714170/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142957155","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sex-associated differences in incentive salience and drinking behaviour in a rodent model of alcohol relapse","authors":"Aileen Hakus,&nbsp;Jerome Clifford Foo,&nbsp;Marta Casquero-Veiga,&nbsp;Asude Zülal Gül,&nbsp;Franziska Hintz,&nbsp;Marion Rivalan,&nbsp;York Winter,&nbsp;Josef Priller,&nbsp;Ravit Hadar,&nbsp;Christine Winter","doi":"10.1111/adb.70009","DOIUrl":"10.1111/adb.70009","url":null,"abstract":"<p>The ability of environmental cues to trigger alcohol-seeking behaviours is thought to facilitate problematic alcohol use. Individuals' tendency to attribute incentive salience to cues may increase the risk of addiction. We sought to study the relationship between incentive salience and alcohol addiction using non-preferring rats to model the heterogeneity of human alcohol consumption, investigating both males and females. Adult rats were subjected to the alcohol deprivation effect (ADE) paradigm, where they were given voluntary access to different alcohol solutions with repeated interruptions by deprivation and reintroduction phases over a protracted period (five Alcohol Deprivation Cycles). Before each Alcohol Deprivation Cycle, rats were tested in the Pavlovian Conditioned Approach (PCA) paradigm, which quantifies the individual salience toward a conditional cue and the reward, thus allowing us to trace the process of attributing incentive salience to reward cues. During the final Alcohol Deprivation Cycle (ADE5), animals were tested for compulsive-like behaviour using quinine taste adulteration. We investigated sex differences in drinking behaviour and PCA performance. We observed thatb females drank significantly more alcohol than males and displayed more sign-tracking (ST) behaviour in the PCA, whereas males showed goal-tracking (GT) behaviour. Furthermore, we found that high drinkers exhibited more ST behaviour. The initial PCA phenotype was correlated with later alcohol consumption. Our findings indicate a complex relationship between incentive salience and alcohol addiction and emphasize the importance of considering both sexes in preclinical research.</p>","PeriodicalId":7289,"journal":{"name":"Addiction Biology","volume":"30 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2025-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11705499/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142957126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reduced brain network segregation in alcohol use disorder: Associations with neurocognition","authors":"Xinying Wang,&nbsp;Peter Manza,&nbsp;Xinyi Li,&nbsp;Astrid Ramos-Rolón,&nbsp;Nathan Hager,&nbsp;Gene-Jack Wang,&nbsp;Nora D. Volkow,&nbsp;Yuzheng Hu,&nbsp;Zhenhao Shi,&nbsp;Corinde E. Wiers","doi":"10.1111/adb.13446","DOIUrl":"10.1111/adb.13446","url":null,"abstract":"<p>The human brain consists of functionally segregated networks, characterized by strong connections among regions belonging to the same network and weak connections between those of different networks. Alcohol use disorder (AUD) is associated with premature brain aging and neurocognitive impairments. Given the link between decreased brain network segregation and age-related cognitive decline, we hypothesized lower brain segregation in patients with AUD than healthy controls (HCs). Thirty AUD patients (9 females, 21 males) and 61 HCs (35 females, 26 males) underwent resting-state functional MRI (rs-fMRI), whose data were processed to assess segregation within the brain sensorimotor and association networks. We found that, compared to HCs, AUD patients had significantly lower segregation in both brain networks as well as poorer performance on a spatial working memory task. In the HC group, brain network segregation correlated negatively with age and positively with spatial working memory. Our findings suggest reduced brain network segregation in individuals with AUD that may contribute to cognitive impairment and is consistent with premature brain aging in this population.</p>","PeriodicalId":7289,"journal":{"name":"Addiction Biology","volume":"29 12","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1111/adb.13446","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142840135","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biochemical changes precede affective and cognitive anomalies in aging adult C57BL/6J mice with a prior history of adolescent alcohol binge-drinking","authors":"C. Leonardo Jimenez Chavez,&nbsp;Gavin P. Scheldrup,&nbsp;Lauren E. Madory,&nbsp;Christopher J. E. Denning,&nbsp;Edward C. Lee,&nbsp;Dylan T. Nguyen,&nbsp;Marian Castro,&nbsp;Andrew Garcia,&nbsp;Jose Torres-Gonzales,&nbsp;Jessica N. Herbert,&nbsp;Daniel Kotlyar,&nbsp;Neda Riazat,&nbsp;William Pakter,&nbsp;William Le,&nbsp;Eliyanna Van Doren,&nbsp;Marianna Ter Galstian,&nbsp;Karen K. Szumlinski","doi":"10.1111/adb.70006","DOIUrl":"10.1111/adb.70006","url":null,"abstract":"<p>The early initiation of binge-drinking and biological sex are critical risk factors for the development of affective disturbances and cognitive decline, as well as neurodegenerative diseases including Alzheimer's disease. Further, a history of excessive alcohol consumption alters normal age-related changes in the pattern of protein expression in the brain, which may relate to an acceleration of cognitive decline. Here, we aimed to disentangle the interrelation between a history of binge-drinking during adolescence, biological sex and normal aging on the manifestation of negative affect, cognitive decline and associated biochemical pathology. To this end, adolescent male and female C57BL/6J mice (PND 28–29) underwent 30 days of alcohol binge-drinking using a modified drinking-in-the-dark (DID) paradigm. Then, mice were assayed for negative affect, sensorimotor gating and cognition at three developmental stages during adulthood—mature adulthood (6 months), pre-middle age (9 months) and middle age (12 months). Behavioural testing was then followed by immunoblotting to index the protein expression of glutamate receptors, neuropathological markers [Tau, p (Thr217)-Tau, p (Ser396)-Tau, BACE, APP, Aβ], as well as ERK activation within the entorhinal cortex, prefrontal cortex and amygdala. Across this age span, we detected only a few age-related changes in our measures of negative affect or spatial learning/memory in the Morris water maze and all of these changes were sex-specific. Prior adolescent binge-drinking impaired behaviour only during reversal learning in 9-month-old females and during radial arm maze testing in 12-month-old females. In contrast to behaviour, we detected a large number of protein changes related to prior binge-drinking history, several of which manifested as early as 6 months of age, with the prefrontal cortex particularly affected at this earlier age. While 6-month-old mice exhibited relatively few alcohol-related protein changes within the entorhinal cortex and amygdala, the number of alcohol-related protein changes within the entorhinal cortex increased with age, while the 12-month-old mice exhibited the largest number of protein changes within the amygdala. Approximately a third of the alcohol-related protein changes were sex-selective. Taken together, the results of our longitudinal study using a murine model of binge-drinking indicate that a prior history of heavy alcohol consumption, beginning in adolescence, is sufficient to induce what we presume to be latent changes in protein indices of cellular activity, glutamate transmission and neuropathology within key brain regions governing cognition, executive function and emotion that appear to precede the onset of robust behavioural signs of dysregulated affect and cognitive impairment.</p>","PeriodicalId":7289,"journal":{"name":"Addiction Biology","volume":"29 12","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11635696/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142814624","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Neural responses to stress and alcohol cues in individuals with pain with and without alcohol use disorder","authors":"Milena Radoman,&nbsp;Colleen McGowan,&nbsp;Emily Heilner,&nbsp;Cheryl Lacadie,&nbsp;Rajita Sinha","doi":"10.1111/adb.70010","DOIUrl":"10.1111/adb.70010","url":null,"abstract":"<p>Pain and alcohol use disorder (AUD) frequently co-occur, but the underlying neurobiology is not well-understood. Although many studies have reported disruptions in stress and reward cue-elicited neural reactivity and heightened alcohol craving in individuals with AUD, little is known about these constructs among patients who experience pain. Here, individuals with pain (Pain+, <i>n</i> = 31) and without pain (Pain−, <i>n</i> = 37) completed a well-validated functional magnetic resonance imaging (fMRI) paradigm involving stress (S), alcohol (A) and neutral (N) cue exposure with repeated alcohol craving assessments. Using whole-brain, voxel-based analyses (<i>p</i> &lt; 0.001, whole-brain cluster correction at <i>α</i> &lt; .05), the Pain+ versus Pain− group evidenced greater dorsal anterior cingulate cortex and left amygdala hyperactivation during N, but hypoactivation during the S-N contrast. Additionally, Pain+ exhibited blunted right anterior insular cortex (AIC) during S-N and blunted anteromedial thalamus and left AIC with hyperactive orbitofrontal cortex (OFC) during A-N. Exploratory analyses further revealed that individuals with pain and AUD (<i>n</i> = 17) relative to pain alone (<i>n</i> = 14) showed hyperactive bilateral AIC and hypoactive right dorsal caudate during A-N. Alcohol cue-induced craving, significantly higher in Pain+ (<i>p</i> = 0.03), correlated with blunted right AIC and OFC responses during A-N. In sum, these results provide first evidence of heightened alcohol cue-elicited craving and disrupted stress- and alcohol cue-reactivity within corticostriatal-limbic regions implicated in negative affect and preoccupation/anticipation stages of AUD in those with pain and with comorbid pain and AUD. Future investigations of pain-AUD interaction are needed that include systematic pain assessment and longitudinal designs with larger sample sizes.</p>","PeriodicalId":7289,"journal":{"name":"Addiction Biology","volume":"29 12","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-12-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11632857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142807957","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}