Advanced Therapeutics最新文献_第8页

Puerarin Ameliorates Premature Ovarian Failure by Activation of Mitochondrial Biogenesis, Dynamics, and Mitophagy Through Up-Regulation of SIRT1 葛根素通过上调SIRT1激活线粒体生物发生、动力学和线粒体自噬，改善卵巢早衰

IF 2.6 4区医学

Advanced Therapeutics Pub Date : 2025-03-26 DOI: 10.1002/adtp.202400310

Yuejuan Fang, Limei Huang, Fengyue Li, Xiuli Ding, Hongmei Zhou, Shun Xu

{"title":"Puerarin Ameliorates Premature Ovarian Failure by Activation of Mitochondrial Biogenesis, Dynamics, and Mitophagy Through Up-Regulation of SIRT1","authors":"Yuejuan Fang, Limei Huang, Fengyue Li, Xiuli Ding, Hongmei Zhou, Shun Xu","doi":"10.1002/adtp.202400310","DOIUrl":"10.1002/adtp.202400310","url":null,"abstract":"Premature ovarian failure (POF) is characterized by ovarian atrophy and decreased fertility. Puerarin (Pue), an isoflavone compound, exerts protective effects on ovarian, however, with undefined mechanisms in POF. POF models were established by cyclophosphamide, and Pue and silent information regulator sirtuin 1 (SIRT1) inhibitor Selisistat (EX527) were used. Next, extracted primary rat ovarian granulosa cells (GCs) were treated with EX527 and autophagy activator rapamycin (RA). The data showed that in POF rats, Pue ameliorated estrous cycle, pathological damage, and senescence with higher ovarian index, anti-Müllerianhormone, estradiol, glutathione peroxidase, superoxide dismutase, mitochondrial DNA, and lower luteinizing hormone and follicle-stimulating hormone levels. In GCs, Pue increased cell viability, mitochondrial membrane potential, adenosine triphosphate levels, with decreased senescence and reactive oxygen species. Moreover, Pue induced mitophagy, enhanced peroxisome proliferators-activated receptor-γ coactivator-1alpha (PGC-1α), and reduced malondialdehyde, p16INK4a, p-adenosine 5′-monophosphate (AMP)-activated protein kinase (AMPK)/AMPK, mitochondrial transcription factor A, nuclear respiratory factor 1, dynamin-related protein 1, and mitochondrial fission 1 expression. Notably, the EX527 addition offset the above effects, while RA further reversed effects of EX527. In conclusion, Pue activated mitochondrial biogenesis, dynamics and mitophagy through up-regulation of SIRT1/PGC-1α pathway to ameliorate POF, providing a theoretical support for clinical application of Pue in POF.","PeriodicalId":7284,"journal":{"name":"Advanced Therapeutics","volume":"8 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced Epithelial Cell Uptake of Glycol Chitosan-Coated PLGA Nanoparticles for Oral Drug Delivery 乙二醇壳聚糖包被的PLGA纳米颗粒口服给药增强上皮细胞摄取

IF 2.6 4区医学

Advanced Therapeutics Pub Date : 2025-03-24 DOI: 10.1002/adtp.202400547

Huiwen Pang, Wenxuan Yu, Youzhi Wu, Xuqiang Nie, Guojun Huang, Zhi Ping Xu, Chen Chen, Felicity Y. Han

{"title":"Enhanced Epithelial Cell Uptake of Glycol Chitosan-Coated PLGA Nanoparticles for Oral Drug Delivery","authors":"Huiwen Pang, Wenxuan Yu, Youzhi Wu, Xuqiang Nie, Guojun Huang, Zhi Ping Xu, Chen Chen, Felicity Y. Han","doi":"10.1002/adtp.202400547","DOIUrl":"10.1002/adtp.202400547","url":null,"abstract":"It is reported that poly(lactic-co-glycolic acid) (PLGA) nanoparticles (NPs) coated with chitosan and its derivatives, such as glycol chitosan (GC), can enhance the targeted uptake of PLGA NPs by intestinal epithelial cells. However, the optimal amount of GC for coating and the specific mechanisms by which it facilitates PLGA endocytosis remain unclear. In this study, PLGA-NPs are prepared using either single- or double-emulsion methods and coated with varying amounts of GC. The results confirmed that GC-coated PLGA NPs are internalized via both clathrin-mediated and caveolae-mediated endocytosis, whereas uncoated NPs relied on only clathrin-mediated endocytosis in Caco-2 and HT-29 cells. The optimized GC-coated PLGA-NPs formulation is further modified by layering alginate to enhance the oral delivery of insulin. In subsequent in vivo studies, the GC and alginate-coated PLGA NPs demonstrated stability and prolonged efficacy, achieving approximately a 50% reduction in blood glucose levels at 6 h post-administration in streptozotocin-induced diabetic mice. These findings provide compelling evidence of the optimal coating amount and molecular mechanisms for GC in the PLGA oral platform, underscoring the feasibility and commercial potential of oral delivery platform based on the optimized GC- and alginate-coated PLGA NPs.","PeriodicalId":7284,"journal":{"name":"Advanced Therapeutics","volume":"8 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adtp.202400547","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144309119","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advanced Therapeutic Technologies for Malaria, Tuberculosis, HIV Infection and Neglected Tropical Diseases 疟疾、结核病、艾滋病毒感染和被忽视的热带病的先进治疗技术

IF 2.6 4区医学

Advanced Therapeutics Pub Date : 2025-03-23 DOI: 10.1002/adtp.202500115

Admire Dube, Garry Laverty, Mohammed Balogun

引用次数: 0

Issue Information (Adv. Therap. 3/2025) 发布信息（Adv. Therap. 3/2025）

IF 2.6 4区医学

Advanced Therapeutics Pub Date : 2025-03-19 DOI: 10.1002/adtp.202570007

引用次数: 0

Therapeutic Potential of Inulin-Coated MCT Microcapsules in Modulating the Gut Microbiome for Effective Treatment of Diet-Induced Obesity (Adv. Therap. 3/2025) 菊粉包被的MCT微胶囊调节肠道微生物群有效治疗饮食性肥胖的治疗潜力（Adv. Therap. 3/2025）

IF 2.6 4区医学

Advanced Therapeutics Pub Date : 2025-03-19 DOI: 10.1002/adtp.202570006

Amin Ariaee, Hannah R. Wardill, Anthony Wignall, Aurelia S. Elz, Leah Wright, Clive Prestidge, Paul Joyce

引用次数: 0

Nanoparticles in Allergen-Delivery Systems for Allergen-Specific Immunotherapy (Adv. Therap. 3/2025) 纳米颗粒用于过敏原特异性免疫治疗的过敏原递送系统（Adv. Therap. 3/2025）

IF 2.6 4区医学

Advanced Therapeutics Pub Date : 2025-03-19 DOI: 10.1002/adtp.202570008

Jiann Huey Lee, Rona Chandrawati, N. Alice Lee

引用次数: 0

Antibiotic–Polycationic Peptide Conjugation as an Effective Strategy to Overcome Daptomycin Resistance 抗生素-多阳离子肽偶联是克服达托霉素耐药的有效策略

IF 2.6 4区医学

Advanced Therapeutics Pub Date : 2025-03-18 DOI: 10.1002/adtp.202400473

Sari Rasheed, Florian Umstätter, Eric Mühlberg, Barbro Beijer, Tobias Hertlein, Karel D. Klika, Christian Kleist, Julia Werner, Cornelius Domhan, Mara Bingel, Anna Müller, Marvin Rausch, Stefan Zimmermann, Knut Ohlsen, Uwe Haberkorn, Marcus Koch, Markus Bischoff, Tanja Schneider, Rolf Müller, Jennifer Herrmann, Walter Mier, Philipp Uhl

{"title":"Antibiotic–Polycationic Peptide Conjugation as an Effective Strategy to Overcome Daptomycin Resistance","authors":"Sari Rasheed, Florian Umstätter, Eric Mühlberg, Barbro Beijer, Tobias Hertlein, Karel D. Klika, Christian Kleist, Julia Werner, Cornelius Domhan, Mara Bingel, Anna Müller, Marvin Rausch, Stefan Zimmermann, Knut Ohlsen, Uwe Haberkorn, Marcus Koch, Markus Bischoff, Tanja Schneider, Rolf Müller, Jennifer Herrmann, Walter Mier, Philipp Uhl","doi":"10.1002/adtp.202400473","DOIUrl":"10.1002/adtp.202400473","url":null,"abstract":"The benefit that antibiotics confer to the welfare of mankind is threatened by bacterial resistance. Resistance to daptomycin, a cyclic lipopeptide frequently used for the treatment of complicated bacteremia, is a prime example of this alarming situation. As the restricted number of antibacterial drug targets limits de novo development, chemical modification of existing compounds represents an alternative development option for future antimicrobials. This approach involves altering compounds to target bacteria through multiple mechanisms and/or to reinforce them against resistant strains. Herein, the conjugation of polycationic peptides to daptomycin enhances its effectiveness against a highly daptomycin-resistant laboratory strain of Staphylococcus aureus and clinical isolates of Enterococcus faecium with reduced daptomycin sensitivity. Notably, unlike daptomycin, the activity of these conjugates does not necessarily depend on the calcium concentration. In addition to regaining bacteriolytic activity, the findings indicate the acquisition of an additional or amended mode of action as evidenced by pore formation and the disruption of membrane potential. The combination of enhanced in vitro potency, in vivo activity, and tolerability highlights the potential of this drug modification strategy in combating multidrug-resistant bacteria.","PeriodicalId":7284,"journal":{"name":"Advanced Therapeutics","volume":"8 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adtp.202400473","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944969","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

On the Relentless Pursuit of an Oral Insulin Delivery System: How Far is Too Far? 对口服胰岛素输送系统的不懈追求：到底有多远？

IF 2.6 4区医学

Advanced Therapeutics Pub Date : 2025-03-16 DOI: 10.1002/adtp.202400566

Sourav Bhattacharjee

{"title":"On the Relentless Pursuit of an Oral Insulin Delivery System: How Far is Too Far?","authors":"Sourav Bhattacharjee","doi":"10.1002/adtp.202400566","DOIUrl":"10.1002/adtp.202400566","url":null,"abstract":"As the scientific community celebrates the centenary year of insulin discovery, developing oral insulin delivery systems remains challenging, with disappointing progress. On the contrary, injectable formulations, despite initial setbacks due to poor patient compliance and issues with painful daily injections, have improved considerably—and currently remain the mainstay of insulin therapeutics. Advanced microneedle technology has enabled insulin delivery with minimal nociception in conjunction with automated, user-friendly delivery platforms. Furthermore, integrating modalities like insulin pumps delivering precise doses based on blood glucose monitoring has emerged. Conversely, oral insulin delivery continues to face arcane challenges, including the denaturation of insulin due to acidic gastric juice, a hostile gut mucus barrier that immobilizes and then removes particulate formulations, and a portal circulation that shunts the residue minuscule (nano)particulate dose from the bloodstream into the liver followed by macrophage activation and hepatobiliary elimination. Unless these barriers are negotiated, breaking the impasse in oral insulin delivery remains elusive. This perspective argues in favor of focusing solely on injectable insulin while deprioritizing, if not fully ceasing, further research toward developing oral insulin formulations to prevent wasting both taxpayers’ money and unethical consumption of animal lives.","PeriodicalId":7284,"journal":{"name":"Advanced Therapeutics","volume":"8 6","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/adtp.202400566","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144308745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hydrogel Delivering All-Trans Retinoic Acid to Regulate Macrophage Polarization to Enhance Chemo-Immunotherapy for Gastric Cancer 水凝胶输送全反式维甲酸调节巨噬细胞极化增强胃癌化疗免疫治疗

IF 2.6 4区医学

Advanced Therapeutics Pub Date : 2025-03-12 DOI: 10.1002/adtp.202400024

Ruobing Bai, Gang Wang, Boru Hou, Dengfeng Wang, Ruihao Li, Zipeng Xu, Weibin Ma, Hongbin Liu

{"title":"Hydrogel Delivering All-Trans Retinoic Acid to Regulate Macrophage Polarization to Enhance Chemo-Immunotherapy for Gastric Cancer","authors":"Ruobing Bai, Gang Wang, Boru Hou, Dengfeng Wang, Ruihao Li, Zipeng Xu, Weibin Ma, Hongbin Liu","doi":"10.1002/adtp.202400024","DOIUrl":"10.1002/adtp.202400024","url":null,"abstract":"As Gastric cancer is one of the most common gastrointestinal malignancies in China, with a 5-year relative survival rate of ≈40%. Therefore, the development of new strategies to treat gastric cancer becomes urgent. In recent years, an increasing number of studies have found that all-trans retinoic acid (Tre) can induce the polarization of M2 macrophages toward M1 in the tumor immune microenvironment (TIME), and therefore play a due role in this cancer treatment. This research proposes to load doxorubicin (DOX) and Tre in mesoporous silica, which is then loaded into sodium alginate slow-release Gel to obtain the final product (GEL-MSDT). Gel-MSDT sustained-release hydrogel can release DOX and Tre locally in tumor, kill tumor cells, induce tumor immunogenic death, regulate tumor-associated macrophage phenotype, and promote anti-tumor immune response. Gel-MSDT hydrogel can coordinate chemotherapy with immunotherapy, and delay release locally to play a lasting anti-tumor immune effect. The results of in vitro and in vivo experiments show that hydrogel can significantly inhibit tumor growth, providing an effective new strategy for the treatment of gastric cancer.","PeriodicalId":7284,"journal":{"name":"Advanced Therapeutics","volume":"8 5","pages":""},"PeriodicalIF":2.6,"publicationDate":"2025-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143944884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative Network Pharmacology and Bulk RNA-Seq Unveil Berberine's Modulation of Mitochondrial Function and Oxidative Stress via SOD2 in Experimental Models of Ovarian Cancer 综合网络药理学和大量RNA-Seq揭示了小檗碱通过SOD2调节卵巢癌实验模型的线粒体功能和氧化应激

IF 2.6 4区医学

Advanced Therapeutics Pub Date : 2025-03-12 DOI: 10.1002/adtp.202400417

Yujie Cheng, Bing Xiong, Jing Guo, Xiao Li, Lingwei Li, Jiajun Wang, Jiale Li, Siqi Liu, Hang Zhou, Lian Wang, Zhongping Cheng

引用次数: 0