Cytoskeleton (Hoboken, N.J.)最新文献_第4页

A truncation mutant of adenomatous polyposis coli impairs apical cell extrusion through elevated epithelial tissue tension. 腺瘤性息肉病大肠杆菌的截短突变体会通过上皮组织张力的升高来影响顶端细胞的挤出。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2024-07-10 DOI: 10.1002/cm.21893

Wan J Gan, Rabina Giri, Jakob Begun, Helen E Abud, Edna C Hardeman, Peter W Gunning, Alpha S Yap, Ivar Noordstra

{"title":"A truncation mutant of adenomatous polyposis coli impairs apical cell extrusion through elevated epithelial tissue tension.","authors":"Wan J Gan, Rabina Giri, Jakob Begun, Helen E Abud, Edna C Hardeman, Peter W Gunning, Alpha S Yap, Ivar Noordstra","doi":"10.1002/cm.21893","DOIUrl":"https://doi.org/10.1002/cm.21893","url":null,"abstract":"Tissue tension encompasses the mechanical forces exerted on solid tissues within animal bodies, originating from various sources such as cellular contractility, interactions with neighboring cells and the extracellular matrix. Emerging evidence indicates that an imbalance in such forces can influence structural organization, homeostasis, and potentially contribute to disease. For instance, heightened tissue tension can impede apical cell extrusion, leading to the retention of apoptotic or transformed cells. In this study, we investigate the potential role of adenomatous polyposis coli (APC) in modulating tissue tension. Our findings reveal that expression of an APC truncation mutant elevates epithelial tension via the RhoA/ROCK pathway. This elevation induces morphological alterations and hampers apoptotic cell extrusion in cultured epithelial cells and organoids, both of which could be mitigated by pharmacologically restoring the tissue tension. This raises the possibility that APC mutations may exert pathogenetic effects by altering tissue mechanics.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141565310","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhanced RHO-ROCK signaling is associated with CRELD2 production and fibroblast recruitment in cutaneous squamous cell carcinoma. 皮肤鳞状细胞癌中 RHO-ROCK 信号的增强与 CRELD2 的产生和成纤维细胞的招募有关。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2024-07-09 DOI: 10.1002/cm.21894

Alexandra Pittar, Edward J Buckley, Sarah T Boyle, S Jan Ibbetson, Michael S Samuel

{"title":"Enhanced RHO-ROCK signaling is associated with CRELD2 production and fibroblast recruitment in cutaneous squamous cell carcinoma.","authors":"Alexandra Pittar, Edward J Buckley, Sarah T Boyle, S Jan Ibbetson, Michael S Samuel","doi":"10.1002/cm.21894","DOIUrl":"https://doi.org/10.1002/cm.21894","url":null,"abstract":"A key characteristic of cancer cells is their ability to induce changes in their microenvironment that render it permissive to tumor growth, invasion and metastasis. Indeed, these changes are required for tumor progression. Consequently, the tumor microenvironment is emerging as a key source of new targets against cancer, with novel therapies aimed at reversing tumor-promoting changes, reinstating a tumor-hostile microenvironment and suppressing disease progression. RHO-ROCK signaling, and consequent tension within the cellular actomyosin cytoskeleton, regulates a paracrine signaling cascade that establishes a tumor-promoting microenvironment. Here, we show that consistent with our observations in breast cancer, enhanced ROCK activity and consequent production of CRELD2 is associated with the recruitment and tumor-promoting polarization of cancer-associated fibroblasts in cutaneous squamous cell carcinoma. Our observations provide support for the notion that the role of RHO-ROCK signaling in establishing a tumor-promoting microenvironment may be conserved across patients and potentially also different cancer types.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141560445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Introducing our associate editorial board members: An interview with Nikki Reinemann. 介绍我们的副编辑委员会成员：尼基-莱纳曼访谈录

Cytoskeleton (Hoboken, N.J.) Pub Date : 2024-07-05 DOI: 10.1002/cm.21892

Paul Trevorrow, Nikki Reinemann

引用次数: 0

Role of actin-binding proteins in cataract formation. 肌动蛋白结合蛋白在白内障形成中的作用

Cytoskeleton (Hoboken, N.J.) Pub Date : 2024-07-03 DOI: 10.1002/cm.21889

Christina Karakosta, Martina Samiotaki, George Panayotou, Dimitrios Papakonstantinou, Marilita M Moschos

{"title":"Role of actin-binding proteins in cataract formation.","authors":"Christina Karakosta, Martina Samiotaki, George Panayotou, Dimitrios Papakonstantinou, Marilita M Moschos","doi":"10.1002/cm.21889","DOIUrl":"https://doi.org/10.1002/cm.21889","url":null,"abstract":"Introduction: Actin has been implicated in lens opacification; however, the specific actin-related pathways involved in cataracts remain unelucidated. In this study, actin-related proteome changes and signaling pathways involved in the development of cataracts were evaluated.Methods: The anterior capsule and phacoemulsification (phaco) cassette contents were collected during cataract surgery from 11 patients with diabetic cataract (DC), 12 patients with age-related cataract (ARC), and seven patients with post-vitrectomy cataract (PVC). Untargeted, global identification and quantification of proteins was performed through liquid chromatography-mass spectrometry with the data-independent acquisition (DIA).Results: In phaco cassette samples, proteins with significantly lower expression in ARC than in DC and PVC were involved in various pathways, including actin binding, actin cytoskeleton reorganization, actin filament capping, cortical actin cytoskeleton organization, and small GTPase-mediated signal transduction pathways. In anterior capsules, proteins with significantly lower expression in ARC than in DC and PVC were involved in actin binding and actin cytoskeleton reorganization pathways.Conclusion: Actin cytoskeleton and actin-binding proteins are involved in lens fiber elongation and differentiation. Rho GTPases contribute to actin cytoskeletal reorganization, and their inactivation is linked to abnormal lens fiber migration. These findings link actin binding to lens fiber integrity, lens opacification, and cataracts.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141494451","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Axonemal tubules in the distal sperm tail of Wolbachia-infected Drosophila simulans males contain ring-like intraluminal structures that persist after axoneme fragmentation. 受狼巴西亚病毒感染的雄果蝇精子尾部远端轴突小管中含有环状内腔结构，这些结构在轴突破碎后仍然存在。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2024-06-24 DOI: 10.1002/cm.21891

Ambra Pratelli, Maria Giovanna Riparbelli, Giuliano Callaini

{"title":"Axonemal tubules in the distal sperm tail of Wolbachia-infected Drosophila simulans males contain ring-like intraluminal structures that persist after axoneme fragmentation.","authors":"Ambra Pratelli, Maria Giovanna Riparbelli, Giuliano Callaini","doi":"10.1002/cm.21891","DOIUrl":"https://doi.org/10.1002/cm.21891","url":null,"abstract":"Wolbachia are obligate intracellular alphaproteobacteria that enhance their spreading by altering the reproductive mechanisms of several invertebrates. Among the reproductive alterations, Wolbachia also causes cytoplasmic incompatibility that leads to embryo death when infected males are crossed with uninfected females, thus selecting infected females. However, the presence of Wolbachia has important fitness costs and infected Drosophila simulans males produce less sperm than their uninfected counterparts. Such sperm suffer, indeed, of some structural alterations that hinder their proper function. We took advantage of the fact that several sperm have abnormal distal regions of the tail, in which the plasma membrane is broken and the axonemal components splayed, making the ultrastructural aspects clearly observable. We found that axoneme reduction in the distal region of the sperm does not follow a unique pattern as observed in other insects, but occurs by losing accessory tubules or peripheral doublets. The axonemal tubules contain distinct coaxial ring-like structures that are still observed after axoneme fragmentation and form large clusters of several units.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141461139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microtubule shaft integrity emerges as a crucial determinant of the acetylation pattern. 微管轴的完整性成为乙酰化模式的关键决定因素。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2024-06-24 DOI: 10.1002/cm.21887

Mireia Andreu-Carbó, Cornelia Egoldt, Charlotte Aumeier

引用次数: 0

Single molecule visualization of tropomyosin isoform organization in the mammalian actin cytoskeleton. 哺乳动物肌动蛋白细胞骨架中肌球蛋白同工酶组织的单分子可视化。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2024-06-14 DOI: 10.1002/cm.21883

Maria L Cagigas, Nicholas Ariotti, Jeff Hook, James Rae, Robert G Parton, Nicole S Bryce, Peter W Gunning, Edna C Hardeman

{"title":"Single molecule visualization of tropomyosin isoform organization in the mammalian actin cytoskeleton.","authors":"Maria L Cagigas, Nicholas Ariotti, Jeff Hook, James Rae, Robert G Parton, Nicole S Bryce, Peter W Gunning, Edna C Hardeman","doi":"10.1002/cm.21883","DOIUrl":"https://doi.org/10.1002/cm.21883","url":null,"abstract":"The actin cytoskeleton is composed of both branched and unbranched actin filaments. In mammals, the unbranched actin filaments are primarily copolymers of actin and tropomyosin. Biochemical and imaging studies indicate that different tropomyosin isoforms are segregated to different actin filament populations in cells and tissues, providing isoform-specific functionality to the actin filament. Intrinsic to this model is the prediction that single-molecule imaging of tropomyosin isoforms would confirm homopolymer formation along the length of single actin filaments, a knowledge gap that remains unaddressed in the cellular environment. We combined chemical labeling of genetically engineered tropomyosin isoforms with electron tomography to locate individual tropomyosin molecules in fibroblasts. We find that the organization of two non-muscle tropomyosins, Tpm3.1 with Tpm4.2, can be distinguished from each other using light and electron microscopy. Visualization of single tropomyosin molecules associated with actin filaments supports the hypothesis that tropomyosins form continuous homopolymers, instead of heteropolymers, in the presence of all physiologically native actin-binding proteins. This is true for both isoforms tested. Furthermore, the data suggest that the tropomyosin molecules on one side of an actin filament may not be in register with those on the opposite side, indicating that each tropomyosin polymer may assembly independently.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141319157","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

MEK inhibitors and DA-Raf, a dominant-negative antagonist of the Ras-ERK pathway, prevent the migration and invasion of KRAS-mutant cancer cells. MEK抑制剂和Ras-ERK通路显性阴性拮抗剂DA-Raf能阻止KRAS突变癌细胞的迁移和侵袭。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2024-06-14 DOI: 10.1002/cm.21881

Aoi Matsuda, Ryuichi Masuzawa, Kazuya Takahashi, Kazunori Takano, Takeshi Endo

{"title":"MEK inhibitors and DA-Raf, a dominant-negative antagonist of the Ras-ERK pathway, prevent the migration and invasion of KRAS-mutant cancer cells.","authors":"Aoi Matsuda, Ryuichi Masuzawa, Kazuya Takahashi, Kazunori Takano, Takeshi Endo","doi":"10.1002/cm.21881","DOIUrl":"https://doi.org/10.1002/cm.21881","url":null,"abstract":"The Ras-induced ERK pathway (Raf-MEK-ERK signaling cascade) regulates a variety of cellular responses including cell proliferation, survival, and migration. Activating mutations in RAS genes, particularly in the KRAS gene, constitutively activate the ERK pathway, resulting in tumorigenesis, cancer cell invasion, and metastasis. DA-Raf1 (DA-Raf) is a splicing isoform of A-Raf and contains the Ras-binding domain but lacks the kinase domain. Consequently, DA-Raf antagonizes the Ras-ERK pathway in a dominant-negative manner and can serve as a tumor suppressor that targets mutant Ras protein-induced tumorigenesis. We show here that MEK inhibitors and DA-Raf interfere with the in vitro collective cell migration and invasion of human KRAS-mutant carcinoma cell lines, the lung adenocarcinoma A549, colorectal carcinoma HCT116, and pancreatic carcinoma MIA PaCa-2 cells. DA-Raf expression was silenced in these cancer cell lines. All these cell lines had high collective migration abilities and invasion properties in Matrigel, compared with nontumor cells. Their migration and invasion abilities were impaired by suppressing the ERK pathway with the MEK inhibitors U0126 and trametinib, an approved anticancer drug. Expression of DA-Raf in MIA PaCa-2 cells reduced the ERK activity and hindered the migration and invasion abilities. Therefore, DA-Raf may function as an invasion suppressor protein in the KRAS-mutant cancer cells by blocking the Ras-ERK pathway when DA-Raf expression is induced in invasive cancer cells.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-06-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141319156","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparative analysis of paxillin and Hic-5 proximity interactomes. paxillin 和 Hic-5 邻近相互作用组的比较分析。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2024-05-27 DOI: 10.1002/cm.21878

Katia Brock, Kyle M Alpha, Grant Brennan, Ebbing P De Jong, Elizabeth Luke, Christopher E Turner

{"title":"A comparative analysis of paxillin and Hic-5 proximity interactomes.","authors":"Katia Brock, Kyle M Alpha, Grant Brennan, Ebbing P De Jong, Elizabeth Luke, Christopher E Turner","doi":"10.1002/cm.21878","DOIUrl":"10.1002/cm.21878","url":null,"abstract":"Focal adhesions serve as structural and signaling hubs, facilitating bidirectional communication at the cell-extracellular matrix interface. Paxillin and the related Hic-5 (TGFβ1i1) are adaptor/scaffold proteins that recruit numerous structural and regulatory proteins to focal adhesions, where they perform both overlapping and discrete functions. In this study, paxillin and Hic-5 were expressed in U2OS osteosarcoma cells as biotin ligase (BioID2) fusion proteins and used as bait proteins for proximity-dependent biotinylation in order to directly compare their respective interactomes. The fusion proteins localized to both focal adhesions and the centrosome, resulting in biotinylation of components of each of these structures. Biotinylated proteins were purified and analyzed by mass spectrometry. The list of proximity interactors for paxillin and Hic-5 comprised numerous shared core focal adhesion proteins that likely contribute to their similar functions in cell adhesion and migration, as well as proteins unique to paxillin and Hic-5 that have been previously localized to focal adhesions, the centrosome, or the nucleus. Western blotting confirmed biotinylation and enrichment of FAK and vinculin, known interactors of Hic-5 and paxillin, as well as several potentially unique proximity interactors of Hic-5 and paxillin, including septin 7 and ponsin, respectively. Further investigation into the functional relationship between the unique interactors and Hic-5 or paxillin may yield novel insights into their distinct roles in cell migration.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11599474/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141155321","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Nucleating amoeboid cancer cell motility with Diaphanous related formins. 用与Diaphanous相关的甲形蛋白核化变形虫癌细胞的运动性。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2024-05-18 DOI: 10.1002/cm.21880

Neelakshi Kar, Jeremy S Logue

引用次数: 0