Cytoskeleton (Hoboken, N.J.)最新文献

Dynein: A Multifaceted Therapeutic Target and Its Dysregulation in Aberrant Cell Proliferation. 动力蛋白：一个多方面的治疗靶点及其在异常细胞增殖中的失调。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-05-26 DOI: 10.1002/cm.22041

Jivesh Garg, Laleh Alisaraie

引用次数: 0

Meet Our Editorial Advisory Board-An Interview With Julien Berro, Yale University, West Haven, CT, USA. 与我们的编辑顾问委员会会面——采访美国康涅狄格州西黑文耶鲁大学的朱利安·贝罗。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-05-21 DOI: 10.1002/cm.21941

Julien Berro, Paul Trevorrow

引用次数: 0

Dissecting the Structural Organization, Recruitment and Activation Mechanisms of Centrosomal γ-TuRCs. 解析中心体γ- turc的结构组织、募集和激活机制。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-05-12 DOI: 10.1002/cm.22040

Florian W Hofer, Martin Würtz, Qi Gao, Bram J A Vermeulen, Elmar Schiebel, Stefan Pfeffer

引用次数: 0

Cytoskeleton Spotlight: Dominik Brokatzky, PhD. 聚焦：Dominik Brokatzky博士。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-05-12 DOI: 10.1002/cm.22036

Dominik Brokatzky

引用次数: 0

Mechanical Coupling With the Nuclear Envelope Shapes the Schizosaccharomyces pombe Mitotic Spindle. 与核膜的机械耦合形成裂糖菌的有丝分裂纺锤体。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-05-10 DOI: 10.1002/cm.22035

Marcus A Begley, Taylor Mahoney, Christian Pagán Medina, Parsa Zareiesfandabadi, Matthew B Rapp, Mastawal Tirfe, Sharonda J LeBlanc, Meredith D Betterton, Mary Williard Elting

{"title":"Mechanical Coupling With the Nuclear Envelope Shapes the Schizosaccharomyces pombe Mitotic Spindle.","authors":"Marcus A Begley, Taylor Mahoney, Christian Pagán Medina, Parsa Zareiesfandabadi, Matthew B Rapp, Mastawal Tirfe, Sharonda J LeBlanc, Meredith D Betterton, Mary Williard Elting","doi":"10.1002/cm.22035","DOIUrl":"https://doi.org/10.1002/cm.22035","url":null,"abstract":"The fission yeast Schizosaccharomyces pombe divides via closed mitosis, meaning that spindle elongation and chromosome segregation transpire entirely within the closed nuclear envelope. Both the spindle and nuclear envelope must undergo shape changes and exert varying forces on each other during this process. Previous work has demonstrated that nuclear envelope expansion (Yam, He, Zhang, Chiam, & Oliferenko, 2011; Mori & Oliferenko, 2020) and spindle pole body (SPB) embedding in the nuclear envelope are required for normal S. pombe mitosis, and mechanical modeling has described potential contributions of the spindle to nuclear morphology (Fang et al., 2020; Zhu et al., 2016). However, it is not yet fully clear how and to what extent the nuclear envelope and mitotic spindle each directly shape each other during closed mitosis. Here, we investigate this relationship by observing the behaviors of spindles and nuclei in live mitotic fission yeast following laser ablation. First, we characterize these dynamics in mitotic S. pombe nuclei with increased envelope tension, finding that nuclear envelope tension can both bend the spindle and slow elongation. Next, we directly probe the mechanical connection between spindles and nuclear envelopes by ablating each structure. We demonstrate that envelope tension can be relieved by severing spindles and that spindle compression can be relieved by rupturing the envelope. We interpret our experimental data via two quantitative models that demonstrate that fission yeast spindles and nuclear envelopes are a mechanical pair that can each shape the other's morphology.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144013002","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The NLS3 Motif in TPX2 Regulates Spindle Architecture in Xenopus Egg Extracts. TPX2中的NLS3 Motif调控爪蟾卵提取物的纺锤体结构。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-05-06 DOI: 10.1002/cm.22034

Guadalupe E Pena, Xiao Zhou, Lauren Slevin, Christopher Brownlee, Rebecca Heald

{"title":"The NLS3 Motif in TPX2 Regulates Spindle Architecture in Xenopus Egg Extracts.","authors":"Guadalupe E Pena, Xiao Zhou, Lauren Slevin, Christopher Brownlee, Rebecca Heald","doi":"10.1002/cm.22034","DOIUrl":"https://doi.org/10.1002/cm.22034","url":null,"abstract":"A bipolar spindle composed of microtubules and many associated proteins functions to segregate chromosomes during cell division in all eukaryotes, yet both spindle size and architecture vary dramatically across different species and cell types. Targeting protein for Xklp2 (TPX2) is one candidate factor for modulating spindle microtubule organization through its roles in branching microtubule nucleation, activation of the mitotic kinase Aurora A, and association with the kinesin-5 (Eg5) motor. Here we characterize a conserved nuclear localization sequence (NLS) motif, 123KKLK126 in Xenopus laevis TPX2, which regulates astral microtubule formation and spindle pole morphology in Xenopus egg extracts. Addition of recombinant TPX2 with this sequence mutated to AALA stimulated spontaneous formation of microtubule asters and increased recruitment of phosphorylated Aurora A, pericentrin, and Eg5 to meiotic spindle poles while still binding to the regulatory transport factor importin α. We propose that TPX2 is a linchpin spindle assembly factor whose regulation contributes to the activation of multiple microtubule polymerizing and organizing proteins, generating distinct spindle architectures.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143998983","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Actin-Bundling Protein, Fascin-1, as a Target of Osteoarthritis Progression. 肌动蛋白捆绑蛋白，筋膜蛋白-1，作为骨关节炎进展的靶标。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-04-23 DOI: 10.1002/cm.22028

Rylee E King, Marin Herrick, Justin Parreno

引用次数: 0

PLK4: Master Regulator of Centriole Duplication and Its Therapeutic Potential. PLK4：中心粒复制的主要调控因子及其治疗潜力。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-04-21 DOI: 10.1002/cm.22031

Muhammad Hamzah, Franz Meitinger, Midori Ohta

{"title":"PLK4: Master Regulator of Centriole Duplication and Its Therapeutic Potential.","authors":"Muhammad Hamzah, Franz Meitinger, Midori Ohta","doi":"10.1002/cm.22031","DOIUrl":"https://doi.org/10.1002/cm.22031","url":null,"abstract":"Centrosomes catalyze the assembly of a microtubule-based bipolar spindle, essential for the precise chromosome segregation during cell division. At the center of this process lies Polo-Like Kinase 4 (PLK4), the master regulator that controls the duplication of the centriolar core to ensure the correct balance of two centrosomes per dividing cell. Disruptions in centrosome number or function can lead to genetic disorders such as primary microcephaly or drive tumorigenesis via centrosome amplification. In this context, several chemical inhibitors of PLK4 have emerged as promising therapeutic candidates. The inhibition of PLK4 results in the emergence of acentrosomal cells, which undergo prolonged and error-prone mitosis. This aberrant mitotic duration triggers a \"mitotic stopwatch\" mechanism that activates the tumor suppressor p53, halting cellular proliferation. However, in a multitude of cancers, the efficacy of this mitotic surveillance mechanism is compromised by mutations that incapacitate p53. Recent investigations have unveiled p53-independent vulnerabilities in cancers characterized by chromosomal gain or amplification of 17q23, which encodes for the ubiquitin ligase TRIM37, in response to PLK4 inhibition, particularly in neuroblastoma and breast cancer. This review encapsulates the latest advancements in our understanding of centriole duplication and acentrosomal cell division in the context of TRIM37 amplification, positioning PLK4 as a compelling target for innovative cancer therapeutics.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144000739","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Inactivation of the Catalytic Activity of Mps1 Kinase Prevents Its Own Degradation at Centrosomes. Mps1激酶催化活性的失活阻止了其在中心体上的降解。

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-04-21 DOI: 10.1002/cm.22032

Shrabani Halder, Arpita Dutta, Rupsa Mondal, Banhi Chowdhury, Benu Brata Das, Shubhra Majumder

引用次数: 0

CEP72 Emerges as a Key Centriolar Satellite Protein in Health and Disease. CEP72成为健康和疾病中的关键中心粒卫星蛋白

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-04-18 DOI: 10.1002/cm.22030

Shweta Tyagi, Aditi Arora, Prajnya Ranganath, Ashwin Dalal

引用次数: 0