Cytoskeleton (Hoboken, N.J.)最新文献_第2页

Picture of the Month by Prabhat Tiwari and Erika Geisbrecht. 月之图由Prabhat Tiwari和Erika Geisbrecht创作。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-09-15 DOI: 10.1002/cm.70043

引用次数: 0

Titin Is Present in the Elastic Tethers That Connect Separating Anaphase Chromosomes in Crane-Fly Spermatocytes. 天蚕精母细胞中连接分离后期染色体的弹性系索中存在Titin。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-09-09 DOI: 10.1002/cm.70035

Demetra Economopoulos, Maral Janan, Martina Krüger, Aavo-Valdur Mikelsaar, Arthur Forer, Rose Sheykhani

引用次数: 0

Structure Makes a Difference: IFT Complex in Ciliary Function and Ciliopathy. 结构决定差异：纤毛功能和纤毛病中的IFT复合体。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-09-05 DOI: 10.1002/cm.70033

Ying Liu, Yong Zhang, Hua Ni, Peiwei Liu

引用次数: 0

O-GlcNAcylation of CEP44 Promotes Its Droplet Formation and Regulates Its Localization. CEP44的o - glcn酰化促进其液滴形成并调节其定位。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-09-04 DOI: 10.1002/cm.70031

Mingzheng Hu, Zihe Zhao, Jinqiong Wang, Ying Shan, Ruming Liu, Weiwen Bu, Dengwen Li, Te Li

{"title":"O-GlcNAcylation of CEP44 Promotes Its Droplet Formation and Regulates Its Localization.","authors":"Mingzheng Hu, Zihe Zhao, Jinqiong Wang, Ying Shan, Ruming Liu, Weiwen Bu, Dengwen Li, Te Li","doi":"10.1002/cm.70031","DOIUrl":"https://doi.org/10.1002/cm.70031","url":null,"abstract":"The centrosomal protein of 44 kDa (CEP44) is essential for centriole duplication, centrosome cohesion, and spindle integrity. It localizes to the proximal end of centrioles and associates with spindle microtubules. Liquid-liquid phase separation (LLPS) is a process by which biomolecules undergo demixing into distinct liquid-like phases, facilitating the formation of cellular condensates such as the centrosome. However, whether CEP44 possesses LLPS properties remains unclear. In this study, we identified intrinsically disordered regions (IDRs) within CEP44, and droplet formation assays confirmed its capacity to form liquid droplets in vivo and in vitro. Immunoblotting detected O-GlcNAcylation of CEP44, indicating its interaction with O-GlcNAc transferase (OGT). Subsequent immunostaining demonstrated that O-GlcNAcylation promotes CEP44 droplet fusion. Post-translational modification prediction analysis suggested a potential interplay between O-GlcNAcylation and phosphorylation that may modulate the structural dynamics of CEP44. Overall, our findings reveal the LLPS capability of CEP44 and underscore the critical role of O-GlcNAcylation in regulating CEP44 droplet fusion and potentially influencing its subcellular localization.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144994509","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tau-Derived Peptides Bearing Azobenzene on Side Chains for Light-Controllable Microtubule Polymerization. 用于光可控微管聚合的侧链上含偶氮苯的tau衍生肽。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-09-01 DOI: 10.1002/cm.70034

Hiroshi Inaba, Misato Umayahara, Akira Kakugo, Kazunori Matsuura

{"title":"Tau-Derived Peptides Bearing Azobenzene on Side Chains for Light-Controllable Microtubule Polymerization.","authors":"Hiroshi Inaba, Misato Umayahara, Akira Kakugo, Kazunori Matsuura","doi":"10.1002/cm.70034","DOIUrl":"https://doi.org/10.1002/cm.70034","url":null,"abstract":"The precise control of microtubule dynamics is essential for diverse cellular processes and is a promising target for optical regulation using photoresponsive molecules. In this study, we developed Tau-derived peptides bearing azobenzene moieties on their side chains that enabled reversible photocontrol of microtubule polymerization by binding to the inside of microtubules. Two peptide derivatives with azobenzene located at different positions were synthesized by simple on-resin Fmoc solid-phase chemistry. Confocal microscopy and competition assays confirmed that both derivatives target the Taxol-binding pocket inside microtubules. Although both cis- and trans-forms bound microtubules, only the cis-form of one derivative (cis-TMR-Azo-TP2) significantly enhanced microtubule polymerization with longer lengths compared with the corresponding trans-form (trans-TMR-Azo-TP2). Moreover, light-dependent switching of microtubule length was achieved via photoisomerization. These findings highlight azobenzene-functionalized Tau-derived peptides as a versatile platform for achieving spatiotemporal control over microtubule polymerization using optical stimuli.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144980938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Discovery of an Atypical Arp2/3 Complex in Malaria Parasites Sheds New Light on Nuclear Actin. 疟原虫非典型Arp2/3复合物的发现为细胞核肌动蛋白的研究提供了新的思路。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-08-19 DOI: 10.1002/cm.70030

Franziska Hentzschel, Friedrich Frischknecht, Matthias Marti

引用次数: 0

Evidence for Motility Determinants in the Kinesin-1 Minimal Motor Core Domain From Tether Variations. 从系链变异来看，Kinesin-1最小运动核心区域运动决定因素的证据。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-08-18 DOI: 10.1002/cm.70029

Rieko Sumiyoshi, Masahiko Yamagishi, Junichiro Yajima

{"title":"Evidence for Motility Determinants in the Kinesin-1 Minimal Motor Core Domain From Tether Variations.","authors":"Rieko Sumiyoshi, Masahiko Yamagishi, Junichiro Yajima","doi":"10.1002/cm.70029","DOIUrl":"https://doi.org/10.1002/cm.70029","url":null,"abstract":"Kinesin-1 is a dimeric motor protein that moves towards the microtubule plus-end in a hand-over-hand fashion. However, the minimal motor domain of kinesin-1 is a single head, and the mechanism by which minimal motor domains generate the force for directional movement remains poorly understood. Here, we engineered artificial tethers (polyethylene glycol, single-stranded DNA, or double-stranded DNA) within the motor domain to investigate whether tether properties such as charge, length, and stiffness affect the motility of teams of kinesin-1 monomers. Neck-linker tethered kinesin with long stiff tethers was found to decrease microtubule-gliding velocity in an in vitro gliding assay, indicating that amplified conformational changes in the neck-linker do not enhance motility. Loop-12 tethered kinesin monomers with various tethers showed consistent minus-end-directed motility, reversing the usual polarity of kinesin-1 monomers. Moreover, loop-3 tethered kinesin monomers switched their directionality depending on tether stiffness. These results indicate that the tether has the potential to influence the direction in which the minimal motor domain moves. We argue that the determinants of motility exist in the minimal motor domain, with the combination of tether properties and its attachment position altering the MT-gliding velocity and direction.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-08-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144877072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Centrosome-Signaling Pathway Crosstalk: A Core Hub From Cellular Homeostasis to Disease. 中心体-信号通路串扰：从细胞稳态到疾病的核心枢纽。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-08-16 DOI: 10.1002/cm.70027

Mingyu Pan, Jinghan Li, Jingyan Fu

引用次数: 0

Post-LECA Origin and Diversification of an Axonemal Outer Arm Dynein Motor. 轴系外臂动力马达的后leca起源与多样化。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-08-08 DOI: 10.1002/cm.70025

Stephen M King

{"title":"Post-LECA Origin and Diversification of an Axonemal Outer Arm Dynein Motor.","authors":"Stephen M King","doi":"10.1002/cm.70025","DOIUrl":"10.1002/cm.70025","url":null,"abstract":"Dyneins were present in the last eukaryotic common ancestor (LECA) and play key roles in eukaryotic biology. Axonemal dyneins form the inner and outer arms that power ciliary beating, and it has long been recognized that outer arms in some organisms contain two different heavy chain motors, whereas those from other species contain a third unit that imparts enhanced motive force during ciliary beating. Previous phylogenetic analyses suggested that this third motor derived from a gene duplication event in the LECA, followed by the subsequent replacement of the N-terminal assembly domain with one formed from kelch and immunoglobulin repeats. Here I revisit the origin and organization of this dynein, combining the increased breadth of sequence information now available, AlphaFold modeling, and the recent recovery of a robustly rooted eukaryotic tree-of-life. This analysis confirms the third outer arm dynein HC arose in a common ancestor of the Diaphoretickes, with a basic N-terminal domain consisting of a β-propeller structure followed by two immunoglobulin folds. However, this region has undergone further diversification in some groups, gaining an additional full or partial β-propeller located immediately adjacent to the AAA motor domain. Thus, three variant forms of this N-terminal segment are discernable in extant eukaryotes.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12338079/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144805411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

ENKD1 Modulates Skin Elasticity Through Microtubule Stability Regulation. ENKD1通过微管稳定性调节皮肤弹性。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2025-08-02 DOI: 10.1002/cm.70016

Dan Dong, Mingzheng Hu, Xiaofan Wu, Ruming Liu, Ying Shan, Tao Zhong, Dengwen Li

引用次数: 0