Cytoskeleton (Hoboken, N.J.)最新文献_第5页

Huntingtin as an Actin Organizer: Structural and Functional Insights. 亨廷顿蛋白作为肌动蛋白的组织者：结构和功能的见解。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2026-05-01 DOI: 10.1002/cm.70143

M Capizzi, S Humbert

引用次数: 0

Optineurin Localization at the Centrosome, Spindle, and Midbody Implies Its Role in Cell Division. 视神经蛋白在中心体、纺锤体和中间体的定位暗示了它在细胞分裂中的作用。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2026-05-01 Epub Date: 2025-07-31 DOI: 10.1002/cm.70015

Ankan Chakraborty, Marie-Noelle Benassy, Dominique Weil, Bishwajit Kundu

{"title":"Optineurin Localization at the Centrosome, Spindle, and Midbody Implies Its Role in Cell Division.","authors":"Ankan Chakraborty, Marie-Noelle Benassy, Dominique Weil, Bishwajit Kundu","doi":"10.1002/cm.70015","DOIUrl":"10.1002/cm.70015","url":null,"abstract":"Optineurin (OPTN), a multifunctional cytosolic protein, is recognized as an autophagy adaptor. Its association with neurodegenerative diseases, like ALS, triggered extensive research. OPTN has been found in intracellular organelles, including the mitochondria, Golgi body, endosomes, microtubules, and the nucleus. The report of mitotic defects and delayed cell division in OPTN-depleted cells prompted us to explore OPTN's exact localization in the cell that could interfere with cell division assemblies. We used three distinct human cell lines, HeLa, HEK293, and SH-SY5Y, and probed them for OPTN localization using both centrosomal (Aurora A kinase, pericentrin, PCM1, Cep170, and γ-tubulin) and mitotic spindle markers (β-tubulin). Our immunofluorescence-based detection using wide-field fluorescence, confocal, and structured illumination microscopy (SIM) placed OPTN at the centrosome, which remained associated with the centriole after duplication and their migration during mitosis. OPTN was also observed at the junction of daughter cells during cytokinesis. Our finding reveals unmapped localizations of OPTN with key cytosolic assemblies that are directly involved in cell division.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":"250-258"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144980875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tau-Derived Peptides Bearing Azobenzene on Side Chains for Light-Controllable Microtubule Polymerization. 用于光可控微管聚合的侧链上含偶氮苯的tau衍生肽。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2026-05-01 Epub Date: 2025-09-01 DOI: 10.1002/cm.70034

Hiroshi Inaba, Misato Umayahara, Akira Kakugo, Kazunori Matsuura

{"title":"Tau-Derived Peptides Bearing Azobenzene on Side Chains for Light-Controllable Microtubule Polymerization.","authors":"Hiroshi Inaba, Misato Umayahara, Akira Kakugo, Kazunori Matsuura","doi":"10.1002/cm.70034","DOIUrl":"10.1002/cm.70034","url":null,"abstract":"The precise control of microtubule dynamics is essential for diverse cellular processes and is a promising target for optical regulation using photoresponsive molecules. In this study, we developed Tau-derived peptides bearing azobenzene moieties on their side chains that enabled reversible photocontrol of microtubule polymerization by binding to the inside of microtubules. Two peptide derivatives with azobenzene located at different positions were synthesized by simple on-resin Fmoc solid-phase chemistry. Confocal microscopy and competition assays confirmed that both derivatives target the Taxol-binding pocket inside microtubules. Although both cis- and trans-forms bound microtubules, only the cis-form of one derivative (cis-TMR-Azo-TP2) significantly enhanced microtubule polymerization with longer lengths compared with the corresponding trans-form (trans-TMR-Azo-TP2). Moreover, light-dependent switching of microtubule length was achieved via photoisomerization. These findings highlight azobenzene-functionalized Tau-derived peptides as a versatile platform for achieving spatiotemporal control over microtubule polymerization using optical stimuli.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":"259-267"},"PeriodicalIF":1.6,"publicationDate":"2026-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144980938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Stick Insect Sperm (Phasmatodea) With Mammalian-Like Basal Bodies. 具有类似哺乳动物基部的节肢昆虫精子。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2026-04-27 DOI: 10.1002/cm.70141

Denise Bonente, Marco Gottardo, David Mercati, Maria Giovanna Riparbelli, Romano Dallai, Giuliano Callaini

引用次数: 0

Diurnal Regulation of Flagellar Length and Swimming Speed in the Red-Tide Raphidophyte Chattonella marina. 赤潮查通菌鞭毛长度和游动速度的日变化规律。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2026-04-24 DOI: 10.1002/cm.70140

Yusaku Fujita, Azusa Kage, Takayuki Nishizaka

{"title":"Diurnal Regulation of Flagellar Length and Swimming Speed in the Red-Tide Raphidophyte Chattonella marina.","authors":"Yusaku Fujita, Azusa Kage, Takayuki Nishizaka","doi":"10.1002/cm.70140","DOIUrl":"https://doi.org/10.1002/cm.70140","url":null,"abstract":"The raphidophyte Chattonella marina is a harmful algal bloom (HAB) species known for its distinct diurnal vertical migration (DVM), a behavior important for its survival and bloom formation. However, the single-cell mechanisms governing this migration remain unclear. In this study, we investigated the swimming characteristics of individual C. marina cells during day (light) and night (dark) periods. We observed a strong positive correlation between the length of the propulsive anterior flagellum and the cell's swimming speed. We discovered that the length distribution of the anterior flagellum is different during the day and at night. We also found that the beat frequency of the anterior flagellum was significantly higher during the day compared to the night. This resulted in faster mean swimming speeds during the light period. To investigate the mechanism of length regulation, we tested the role of intraflagellar transport (IFT) using the IFT dynein inhibitor, ciliobrevin D. Treatment with ciliobrevin D induced a time- and concentration-dependent shortening of the anterior flagellum. This is the first pharmacological evidence to suggest that an IFT-like mechanism may actively control motile flagellar length in C. marina. These findings suggest that C. marina modulates its swimming speed through diurnal changes in both flagellar length and beat frequency, likely as an energy-saving strategy coupled to its DVM.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":"e70140"},"PeriodicalIF":1.6,"publicationDate":"2026-04-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147790778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Apoptosis Induction, Disruption of Cytoskeleton Remodeling and EMT Inhibition as Major Mechanism(s) Underlying the Pleiotropic Action of Withania somnifera in Breast Cancer. 诱导凋亡、破坏细胞骨架重塑和抑制EMT是苦参对乳腺癌多效性作用的主要机制。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2026-04-06 DOI: 10.1002/cm.70131

Aditi Srivastava, Tanveer Ahamad, Aqib Sarfraz, Sahabjada Siddiqui, Feroz Khan, Anchal Trivedi, Mohd Faheem Khan, Aparna Misra, Hari Shankar, Mohsin Ali Khan, Rumana Ahmad

{"title":"Apoptosis Induction, Disruption of Cytoskeleton Remodeling and EMT Inhibition as Major Mechanism(s) Underlying the Pleiotropic Action of Withania somnifera in Breast Cancer.","authors":"Aditi Srivastava, Tanveer Ahamad, Aqib Sarfraz, Sahabjada Siddiqui, Feroz Khan, Anchal Trivedi, Mohd Faheem Khan, Aparna Misra, Hari Shankar, Mohsin Ali Khan, Rumana Ahmad","doi":"10.1002/cm.70131","DOIUrl":"https://doi.org/10.1002/cm.70131","url":null,"abstract":"The role of actin and its binding proteins has been discovered in cytoskeleton remodeling as well as in Epithelial-Mesenchymal Transition (EMT) of metastatic cells and apoptosis. Even minor changes in the biomolecular structure of actin and its ABPs (by binding of ligands) can lead to drastic changes in the cytoskeleton with far reaching effects per se. Agents targeting actin can, thus, be viewed as potential anti-metastatic agents. The effect of Withania somnifera (L.) Dunal (WS) on the cytoskeleton has remained relatively unexplored. The present study highlights the interaction between 20 WS phytoconstituents and 10 selected cytoskeletal proteins in silico with a view to validate and analyze the perturbation in growth and differentiation of breast cancer cells in vitro. Pharmacokinetic analyses revealed that the majority of WS phytoconstituents exhibited no violations of Lipinski's rule-of-five parameters. Withanolides A, B, D, M and O displayed the greatest binding affinity particularly for coronin1A, vimentin, gelsolin, ezrin and F-actin. MD simulations of 100 ns revealed maximum stable interaction(s) between Coronin-Viscosalactone B (VISCB) and Vimentin-Withanolide E (WITHE). The prepared methanolic extract of WS stem (WSME), characterized using LC-MS, revealed the presence of Withaferin A (WFA). Both WSME and WFA exhibited potent cytotoxicity against breast cancer MDA-MB-231 cells. WSME increased ROS levels, arrested the cell cycle in S and G2-M phases, decreased the expression of mesenchymal markers, namely, vimentin, N-cadherin and increased levels of the epithelial marker E-cadherin in treated MDA-MB-231 cells. These findings suggest that VISCB, WITHE, and WFA have the potential to emerge as potential antimetastatic agents against breast cancer in the future.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":""},"PeriodicalIF":1.6,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147629320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The C-Terminal Regions of TRAK Proteins Contain MIRO-Independent Mitochondrial Localization Domains. TRAK蛋白的c端区域含有微独立的线粒体定位域。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2026-04-01 Epub Date: 2025-10-23 DOI: 10.1002/cm.70061

Lili Mitchell, Kathryn E Reda, Hijab Fatima, Claudia E Vasquez, Omar A Quintero-Carmona

{"title":"The C-Terminal Regions of TRAK Proteins Contain MIRO-Independent Mitochondrial Localization Domains.","authors":"Lili Mitchell, Kathryn E Reda, Hijab Fatima, Claudia E Vasquez, Omar A Quintero-Carmona","doi":"10.1002/cm.70061","DOIUrl":"10.1002/cm.70061","url":null,"abstract":"Current models suggest that MIRO GTPases anchor cytoskeletal motors to the mitochondrial outer membrane (MOM). However, our previous findings indicate that the unconventional myosin, MYO19, interacts with MIRO weakly and that a MIRO-independent MOM-localizing domain interacts more tightly with the MOM. To test the hypothesis that other MIRO interactors may also have MIRO-independent MOM binding, we examined interactions between TRAK proteins (microtubule motor-mitochondria adaptor proteins) and the MOM via quantitative fluorescence microscopy and steady-state kinetic approaches. Using GFP-TRAK truncations expressed in MIRO1-2 double knockout mouse embryonic fibroblasts, we identified a MIRO-independent mitochondrial-binding domain in the C-terminus of TRAK1 and TRAK2, with a MOM localization pattern similar to what we observed for full-length GFP-TRAK proteins. The MIRO-binding domains (MBD) of the TRAK proteins were only able to localize to mitochondria when MIRO is expressed. Importantly, fluorescence recovery after photobleaching (FRAP) demonstrated that the steady-state kinetics of TRAKMBD/MIRO interactions were faster exchanging than for either full-length TRAK or the TRAK C-terminal MOM-binding domain expressed alone. These data support a model where TRAK/MIRO associations may be serving functions beyond anchoring cytoskeletal motors and their adapters to the MOM.","PeriodicalId":72766,"journal":{"name":"Cytoskeleton (Hoboken, N.J.)","volume":" ","pages":"187-199"},"PeriodicalIF":1.6,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13080069/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145350411","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

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Author Profile: Dr. Dominik Brokatzky. 作者简介：Dominik Brokatzky博士。

IF 1.6

Cytoskeleton (Hoboken, N.J.) Pub Date : 2026-04-01 Epub Date: 2026-03-31 DOI: 10.1002/cm.70133

Dominik Brokatzky

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Introduction to the Special Issue "Cytoskeleton Regulation of Mitochondrial Dynamics". 特刊“线粒体动力学的细胞骨架调节”简介。

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Cytoskeleton (Hoboken, N.J.) Pub Date : 2026-04-01 Epub Date: 2026-02-18 DOI: 10.1002/cm.70111

David Gau

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Author Profile (Trainee)-Shivani Tuli. 作者简介（实习生）-Shivani Tuli。