Clinical and diagnostic laboratory immunology最新文献

{"title":"Longitudinal analysis of Severe Acute Respiratory Syndrome (SARS) coronavirus-specific antibody in SARS patients.","authors":"Shan-Chwen Chang,&nbsp;Jann-Tay Wang,&nbsp;Li-Min Huang,&nbsp;Yee-Chun Chen,&nbsp;Chi-Tai Fang,&nbsp;Wang-Huei Sheng,&nbsp;Jiun-Ling Wang,&nbsp;Chong-Jen Yu,&nbsp;Pan-Chyr Yang","doi":"10.1128/CDLI.12.12.1455-1457.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.12.1455-1457.2005","url":null,"abstract":"<p><p>The serum antibodies to severe acute respiratory syndrome (SARS) coronavirus of 18 SARS patients were checked at 1 month and every 3 months after disease onset. All of them except one, who missed blood sampling at 1 month, tested positive for the immunoglobulin G (IgG) antibody at 1 month. Fifteen out of 17 tested positive for the IgM antibody at 1 month. The serum IgM antibody of most patients became undetectable within 6 months after the onset of SARS. The IgG antibody of all 17 patients, whose serum was checked 1 year after disease onset, remained positive.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 12","pages":"1455-7"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.12.1455-1457.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25734745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boosting immune response to hepatitis B DNA vaccine by coadministration of Prothymosin alpha-expressing plasmid.","authors":"Yanwen Jin,&nbsp;Cheng Cao,&nbsp;Ping Li,&nbsp;Xuan Liu,&nbsp;Wei Huang,&nbsp;Chufang Li,&nbsp;Qingjun Ma","doi":"10.1128/CDLI.12.12.1364-1369.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.12.1364-1369.2005","url":null,"abstract":"<p><p>DNA vaccines induce protective humoral and cell-mediated immune responses in several animal models. However, compared to conventional vaccines, DNA vaccines usually induce poor antibody responses. In this study, we report that coadministration of a hepatitis B virus (HBV) DNA vaccine with prothymosin alpha as an adjuvant improves antibody responses to HBV S antigen. We also observed higher seroconversion rates and higher antibody titers. Prothymosin alpha appears to increase the number and affinity of hepatitis B surface antigen-specific, gamma interferon-secreting T cells and to enhance cellular immune response to the PreS2S DNA vaccine. Interestingly, administering the DNA separately from the prothymosin alpha plasmid abrogated the enhancement of DNA vaccine potency. The results suggest that prothymosin alpha may be a promising adjuvant for DNA vaccines.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 12","pages":"1364-9"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.12.1364-1369.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25732124","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term follow-up of Hepatitis B Surface antibody levels in subjects receiving universal Hepatitis B vaccination in infancy in an area of hyperendemicity: correlation between radioimmunoassay and enzyme immunoassay.","authors":"Ching-Wen Wang,&nbsp;Li-Chieh Wang,&nbsp;Mei-Hwei Chang,&nbsp;Yen-Hsuan Ni,&nbsp;Huey-Ling Chen,&nbsp;Hong-Yuan Hsu,&nbsp;Ding-Shin Chen","doi":"10.1128/CDLI.12.12.1442-1447.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.12.1442-1447.2005","url":null,"abstract":"<p><p>The aims of the present study were to determine (i) the long-term immunogenicity and the decay rate of hepatitis B virus (HBV) surface antibody (anti-HBs) from universal hepatitis B vaccination at infancy for a healthy population in an area of hyperendemicity and (ii) whether the anti-HBs levels measured by enzyme immunoassay (EIA) were closely correlated with those assayed by radioimmunoassay (RIA) methods during long-term monitoring. A total of 1,337 apparently healthy children (696 boys and 641 girls) who were vaccinated against HBV at infancy and monitored for anti-HBs annually from 7 to 16 years of age entered the study. Serum samples were analyzed for anti-HBs by RIA at 7 to 15 years of age and were also analyzed by EIA at 13 to 16 years of age. Antibody titers were quantified in mIU/ml by EIA as well as by the ratio of the count in the sample to the count for a negative control (S/N) by RIA. In non-boosted children, the average decay of anti-HBs from 7 to 16 years of ages indicated that approximately 20% of the geometric mean titer decays per year. There was a good correlation between serum anti-HBs levels measured by the RIA and the EIA methods (r=0.91; P<0.0001). An equation for RIA to EIA level conversion was established: log EIA titer=-0.12+ (1.31 . log RIA S/N). The anti-HBs titers measured by EIA correlate well with the S/N assayed by RIA. The annual decay rate of the log anti-HBs level may help in planning booster immunizations for hypo-responders or individuals at risk in adolescence.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 12","pages":"1442-7"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.12.1442-1447.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25731492","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Computer-assisted pattern recognition of autoantibody results.","authors":"Steven R Binder,&nbsp;Mark C Genovese,&nbsp;Joan T Merrill,&nbsp;Robert I Morris,&nbsp;Allan L Metzger","doi":"10.1128/CDLI.12.12.1353-1357.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.12.1353-1357.2005","url":null,"abstract":"<p><p>Immunoassay-based anti-nuclear antibody (ANA) screens are increasingly used in the initial evaluation of autoimmune disorders, but these tests offer no \"pattern information\" comparable to the information from indirect fluorescence assay-based screens. Thus, there is no indication of \"next steps\" when a positive result is obtained. To improve the utility of immunoassay-based ANA screening, we evaluated a new method that combines a multiplex immunoassay with a k nearest neighbor (kNN) algorithm for computer-assisted pattern recognition. We assembled a training set, consisting of 1,152 sera from patients with various rheumatic diseases and non-diseased patients. The clinical sensitivity and specificity of the multiplex method and algorithm were evaluated with a test set that consisted of 173 sera collected at a rheumatology clinic from patients diagnosed by using standard criteria, as well as 152 age- and sex-matched sera from presumably healthy individuals (sera collected at a blood bank). The test set was also evaluated with a HEp-2 cell-based enzyme-linked immunosorbent assay (ELISA). Both the ELISA and multiplex immunoassay results were positive for 94% of the systemic lupus erythematosus (SLE) patients. The kNN algorithm correctly proposed an SLE pattern for 84% of the antibody-positive SLE patients. For patients with no connective tissue disease, the multiplex method found fewer positive results than the ELISA screen, and no disease was proposed by the kNN algorithm for most of these patients. In conclusion, the automated algorithm could identify SLE patterns and may be useful in the identification of patients who would benefit from early referral to a specialist, as well as patients who do not require further evaluation.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 12","pages":"1353-7"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.12.1353-1357.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25732122","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Modulation of antibody-mediated immune response by probiotics in chickens.","authors":"Hamid R Haghighi,&nbsp;Jianhua Gong,&nbsp;Carlton L Gyles,&nbsp;M Anthony Hayes,&nbsp;Babak Sanei,&nbsp;Payvand Parvizi,&nbsp;Haris Gisavi,&nbsp;James R Chambers,&nbsp;Shayan Sharif","doi":"10.1128/CDLI.12.12.1387-1392.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.12.1387-1392.2005","url":null,"abstract":"<p><p>Probiotic bacteria, including Lactobacillus acidophilus and Bifidobacterium bifidum, have been shown to enhance antibody responses in mammals. The objective of this study was to examine the effects of a probiotic product containing the above bacteria in addition to Streptococcus faecalis on the induction of the chicken antibody response to various antigens, both systemically and in the gut. The birds received probiotics via oral gavage and subsequently were immunized with sheep red blood cells (SRBC) and bovine serum albumin (BSA) to evaluate antibody responses in serum or with tetanus toxoid (TT) to measure the mucosal antibody response in gut contents. Control groups received phosphate-buffered saline. Overall, BSA and SRBC induced a detectable antibody response as early as week 1 postimmunization (p.i.), which lasted until week 3 p.i. Probiotic-treated birds had significantly (P <or= 0.001) more serum antibody (predominantly immunoglobulin M [IgM]) to SRBC than the birds that were not treated with probiotics. However, treatment with probiotics did not enhance the serum IgM and IgG antibody responses to BSA. Immunization with TT resulted in the presence of specific IgA and IgG antibody responses in the gut. Again, treatment with probiotics did not change the level or duration of the antibody response in the gut. In conclusion, probiotics enhance the systemic antibody response to some antigens in chickens, but it remains to be seen whether probiotics have an effect on the generation of the mucosal antibody response.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 12","pages":"1387-92"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.12.1387-1392.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25732127","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Local and systemic immune and inflammatory responses to Helicobacter pylori strains.","authors":"Niranjan Bhat,&nbsp;James Gaensbauer,&nbsp;Richard M Peek,&nbsp;Karen Bloch,&nbsp;Kyi-Toe Tham,&nbsp;Martin J Blaser,&nbsp;Guillermo Perez-Perez","doi":"10.1128/CDLI.12.12.1393-1400.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.12.1393-1400.2005","url":null,"abstract":"<p><p>Colonization with Helicobacter pylori eventuates in varied clinical outcomes, which relate to both bacterial and host factors. Here we examine the relationships between cagA status, serum and gastric juice antibody responses, and gastric inflammation in dyspeptic patients. Serum, gastric juice, and gastric biopsy specimens were obtained from 89 patients undergoing endoscopy. H. pylori colonization and cagA status were determined by histology, culture, and PCR methods, and acute inflammation and chronic inflammation in the gastric mucosa were scored by a single pathologist. Serum and gastric juice antibodies to H. pylori whole-cell and CagA antigens were determined by enzyme-linked immunosorbent assay. Relationships between variables were sequentially analyzed using univariate and multivariate statistical methods. Of the 89 subjects, 62 were colonized by H. pylori. By univariate analyses, levels of serum immunoglobulin G (IgG) and IgA and gastric juice IgA antibodies against whole-cell and CagA antigens each were significantly higher in the H. pylori-positive group than in the H. pylori-negative group (P<0.001). H. pylori and CagA sero-positivities were both significantly associated with enhanced inflammation in gastric antrum and body (P<0.02). The presence of gastric juice antibodies to H. pylori antigens was associated with more severe gastric inflammation. However, in multivariate analyses, only the presence of serum antibodies against CagA and, to a lesser extent, whole-cell antigens remained significantly associated with acute and chronic inflammation in antrum and body (P<0.05). Thus, serum antibody response to CagA correlates with severity of gastric inflammation. Furthermore, given the relationships demonstrated by multivariate analysis, determination of gastric juice antibodies may provide a better representation of serum, rather than secretory, immune response.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 12","pages":"1393-400"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.12.1393-1400.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25732128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation and diagnostic usefulness of domestic and imported enzyme-linked immunosorbent assays for detection of human immunodeficiency virus type 1 antibody in India.","authors":"H Syed Iqbal,&nbsp;Suniti Solomon,&nbsp;K G Murugavel,&nbsp;Sunil Suhas Solomon,&nbsp;P Balakrishnan","doi":"10.1128/CDLI.12.12.1425-1428.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.12.1425-1428.2005","url":null,"abstract":"<p><p>Diagnosis of human immunodeficiency virus (HIV) infection is important for patient management and prevention of new infections. The number of test kits available for the detection of HIV antibodies is unprecedented. In order to identify appropriate test kits, we evaluated a variety of commercial kits manufactured abroad as well as in India. The plasma and serum specimens (n=264) were collected from individuals attending the Voluntary Counseling and Testing Centre at the YRG Centre for AIDS and Education. The specimens were used to evaluate six commercially available HIV test kits: Enzaids HIV 1+2, HIV-CheX, Murex HIV-1.2.0, Genscreen HIV 1/2 version 2, Vironostika HIV Uni-Form II Ag/Ab, and CombAids RS Advantage. High sensitivities and specificities (>or=99%) were observed for the Enzaids, Murex, Vironostika, and CombAids assays. HIV-CheX showed the highest number of false-positive and false-negative results. The Genscreen test also gave many false positives. The study indicated that the Enzaids, Murex, and Vironostika enzyme-linked immunosorbent assay kits and the CombAids RS Advantage rapid assay could be used to achieve acceptable results for the detection of HIV antibodies. A combination of two tests is recommended to optimize the efficiency of HIV antibody testing algorithms, especially when evaluation with an HIV Western blot confirmatory test is not possible.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 12","pages":"1425-8"},"PeriodicalIF":0.0,"publicationDate":"2005-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.12.1425-1428.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25731489","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Serological responses in patients with severe acute respiratory syndrome coronavirus infection and cross-reactivity with human coronaviruses 229E, OC43, and NL63.","authors":"K H Chan,&nbsp;V C C Cheng,&nbsp;P C Y Woo,&nbsp;S K P Lau,&nbsp;L L M Poon,&nbsp;Y Guan,&nbsp;W H Seto,&nbsp;K Y Yuen,&nbsp;J S M Peiris","doi":"10.1128/CDLI.12.11.1317-1321.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.11.1317-1321.2005","url":null,"abstract":"<p><p>The serological response profile of severe acute respiratory syndrome (SARS) coronavirus (CoV) infection was defined by neutralization tests and subclass-specific immunofluorescent (IF) tests using serial sera from 20 patients. SARS CoV total immunoglobulin (Ig) (IgG, IgA, and IgM [IgGAM]) was the first antibody to be detectable. There was no difference in time to seroconversion between the patients who survived (n = 14) and those who died (n = 6). Although SARS CoV IgM was still detectable by IF tests with 8 of 11 patients at 7 months postinfection, the geometric mean titers dropped from 282 at 1 month postinfection to 19 at 7 months (P = 0.001). In contrast, neutralizing antibody and SARS CoV IgGAM and IgG antibody titers remained stable over this period. The SARS CoV antibody response was sometimes associated with an increase in preexisting IF IgG antibody titers for human coronaviruses OC43, 229E, and NL63. There was no change in IF IgG titer for virus capsid antigen from the herpesvirus that was used as an unrelated control, Epstein-Barr virus. In contrast, patients who had OC43 infections, and probably also 229E infections, without prior exposure to SARS CoV had increases of antibodies specific for the infecting virus but not for SARS CoV. There is a need for awareness of cross-reactive antibody responses between coronaviruses when interpreting IF serology.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 11","pages":"1317-21"},"PeriodicalIF":0.0,"publicationDate":"2005-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.11.1317-1321.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25678273","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and characterization of the promoter and partial enhancer region of the porcine inter-alpha-trypsin inhibitor heavy chain 4 gene.","authors":"Niamh Harraghy,&nbsp;Timothy J Mitchell","doi":"10.1128/CDLI.12.11.1336-1339.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.11.1336-1339.2005","url":null,"abstract":"<p><p>A porcine genomic library was screened for clones containing the promoter of the major acute-phase protein in pigs, inter-alpha-trypsin heavy chain 4 (ITIH4). Following isolation of the promoter, a functional analysis was performed with Hep3B cells. The promoter was induced by interleukin-6 (IL-6) but not by IL-1beta. However, IL-1beta was shown to inhibit the IL-6-induced activation of the porcine ITIH4 promoter.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 11","pages":"1336-9"},"PeriodicalIF":0.0,"publicationDate":"2005-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.11.1336-1339.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25681106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A strain-specific antigen in Japanese Helicobacter pylori recognized in sera of Japanese children.","authors":"Masumi Okuda,&nbsp;Toshiro Sugiyama,&nbsp;Kenichi Fukunaga,&nbsp;Masaru Kondou,&nbsp;Eikichi Miyashiro,&nbsp;Teruko Nakazawa","doi":"10.1128/CDLI.12.11.1280-1284.2005","DOIUrl":"https://doi.org/10.1128/CDLI.12.11.1280-1284.2005","url":null,"abstract":"<p><p>An enzyme immuno assay (EIA) test based on Japanese strain-derived high-molecular-weight cell-associated proteins (JHM-CAP) was evaluated by comparing with a previously developed EIA test based on a U.S. strain-derived high-molecular-weight cell-associated proteins (HM-CAP). Serum samples of 131 Japanese asymptomatic children (mean age, 5.5 years; range, 0 to 21 years) were tested that include 43 positive and 88 negative children as judged by Helicobacter pylori stool antigen test (HpSA test). Both tests showed comparable and reliable specificities, but the sensitivity of JHM-CAP EIA, at 93.0%, was much higher than that of HM-CAP EIA, at 67.4%. More false-negative results of HM-CAP were obtained in children under 10 years of age. Immunoblot analysis revealed that the JHM-CAP but not the HM-CAP preparation had a 100-kDa antigen recognized by JHM-CAP positive sera. It was concluded that JHM-CAP EIA is highly accurate for the serodiagnosis of H. pylori infection in Japanese young children and that the high sensitivity of JHM-CAP EIA in contrast to HM-CAP EIA is due to the presence of a 100-kDa antigen in Japanese strains that may be recognized by the host immune system at an early stage of infection.</p>","PeriodicalId":72602,"journal":{"name":"Clinical and diagnostic laboratory immunology","volume":"12 11","pages":"1280-4"},"PeriodicalIF":0.0,"publicationDate":"2005-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1128/CDLI.12.11.1280-1284.2005","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25678267","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}