ChemSystemsChem最新文献_第2页

Easy, Rapid and High-Throughput Production of Single and Multicompartment Liposomes by Vortex Emulsification 漩涡乳化法简便、快速、高通量生产单室和多室脂质体

IF 3.1

ChemSystemsChem Pub Date : 2024-11-25 DOI: 10.1002/syst.202400085

Zhen-Hong Luo, Gong-Yu Shi, Nan-Nan Deng

引用次数: 0

Front Cover: Photostimuli Reach a Selective Intermediate in a Microflow: One-Shot Transformation from a Supramolecular Co-Polymer to a Micro-Disk Structure (ChemSystemsChem 6/2024) 封面光刺激到达微流中的选择性中间体：从超分子共聚物到微盘结构的一次性转化（ChemSystemsChem 6/2024）

IF 3.1

ChemSystemsChem Pub Date : 2024-11-16 DOI: 10.1002/syst.202480601

Akira Kaneyoshi, Shota Nomura, Takato Maeda, Dr. Takahiro Kusukawa, Dr. Yoshihiro Kikkawa, Dr. Munenori Numata

引用次数: 0

Interfacing Complex Coacervates with Natural Cells 复杂凝聚体与天然细胞的接合

IF 3.1

ChemSystemsChem Pub Date : 2024-11-15 DOI: 10.1002/syst.202400071

He Meng, Yanglimin Ji, Yan Qiao

{"title":"Interfacing Complex Coacervates with Natural Cells","authors":"He Meng, Yanglimin Ji, Yan Qiao","doi":"10.1002/syst.202400071","DOIUrl":"https://doi.org/10.1002/syst.202400071","url":null,"abstract":"Coacervates have been investigated as protocells or synthetic cells, as well as subcellular compartments for the creation of new materials, thus bridging the gap between living and non-living systems in materials science, synthetic biology, and bioengineering. Given the design flexibility and simplicity of coacervates, along with the functionality and complexity of natural cells, the interfacing of complex coacervates with natural cells is considered significant for various biotechnological and biomedical applications. In this review, the fundamental mechanisms and underlying theories of coacervate systems are introduced. Recent efforts to interface coacervates with natural cells are summarized in three key scenarios: (i) the integration of coacervates with natural cell components for the living material assembly into protocells; (ii) communication between therapeutic synthetic cells and natural cells for drug delivery and cell repair; and (iii) the formation of intracellular condensates for metabolic regulation, followed by the regulation of their phase transitions for pathological elucidation. Finally, the potential of coacervate-natural cell interfaces is discussed in the context of developing living/synthetic cell constructs, creating precise disease therapy strategies, and advancing programmable metabolic engineering networks.","PeriodicalId":72566,"journal":{"name":"ChemSystemsChem","volume":"7 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-11-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629847","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Designing and Controlling Transient Supramolecular Gels 瞬态超分子凝胶的设计与控制

IF 3.1

ChemSystemsChem Pub Date : 2024-11-05 DOI: 10.1002/syst.202400073

Emma L. Bowley, Simona Bianco, Fin Hallam Stewart, Chloe M. Wallace, Rebecca E. Ginesi, Alex S. Loch, Martin Rosenthal, Andrew J. Smith, Dave J. Adams

引用次数: 0

Supramolecular Systems Chemistry Based on the Interplay Between Peptides and Porphyrins 基于多肽和卟啉相互作用的超分子系统化学

IF 3.1

ChemSystemsChem Pub Date : 2024-10-23 DOI: 10.1002/syst.202400068

Yue Fu, Lian Zhang, Xuehai Yan, Kai Liu

引用次数: 0

Shape Analysis of Biomimetic and Plasma Membrane Vesicles 仿生囊泡与质膜囊泡的形态分析

IF 3.1

ChemSystemsChem Pub Date : 2024-10-23 DOI: 10.1002/syst.202400052

Rajni Kudawla, Harshmeet Kaur, Tanmay Pandey, Tripta Bhatia

{"title":"Shape Analysis of Biomimetic and Plasma Membrane Vesicles","authors":"Rajni Kudawla, Harshmeet Kaur, Tanmay Pandey, Tripta Bhatia","doi":"10.1002/syst.202400052","DOIUrl":"https://doi.org/10.1002/syst.202400052","url":null,"abstract":"Giant membrane vesicles (GUVs) and Giant plasma membrane vesicles (GPMVs) are used as models to study membrane properties. We conducted a comparative study to examine how reducing the volume of vesicles with different lipid compositions, solution symmetries, solution asymmetries, and membrane charges affects their morphology. We used three-dimensional visualization techniques to study the shape of the vesicles. Although the vesicles may not be perfectly spherical, they exhibit some fluctuations in their shape. To understand these variations, we used confocal image stacks for visualization. Our experimental observations show that the membrane′s charge influences the deflation of the GUVs in the presence of trans-bilayer sugar asymmetries. The lipid bilayers of our GUVs have a uniform distribution of lipids in both leaflets, indicating no asymmetry in lipid composition. We induce trans-bilayer asymmetries by exposing each leaflet of the bilayer to different solution compositions. We also estimated and compared the deformation of GPMV extracted from HEK-293 cells with trans-bilayer buffer asymmetries and inherent leaflet compositional asymmetry with biomimetic membranes.","PeriodicalId":72566,"journal":{"name":"ChemSystemsChem","volume":"7 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143118671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spatially Coded Transformations in Gradient-Dependent Protocell Morphogenesis 梯度依赖的原始细胞形态发生中的空间编码转换

IF 3.1

ChemSystemsChem Pub Date : 2024-10-22 DOI: 10.1002/syst.202400064

Shuqi Wu, Wei Ji, Mei Li, Stephen Mann, Liangfei Tian

{"title":"Spatially Coded Transformations in Gradient-Dependent Protocell Morphogenesis","authors":"Shuqi Wu, Wei Ji, Mei Li, Stephen Mann, Liangfei Tian","doi":"10.1002/syst.202400064","DOIUrl":"https://doi.org/10.1002/syst.202400064","url":null,"abstract":"Chemical gradients provide spatiotemporal signaling fields in various cellular processes, driving complex dynamic behaviours such as differentiation and spatial organization. Here we employ opposing gradients of two artificial morphogens (sodium dodecyl sulfate (SDS) and sodium phosphotungstate (polyoxometalate; POM)) to systematically investigate morphological differentiation in organized populations of coacervate microdroplet-based protocells. Using a matrix of 16 sets of counter-diffusive gradients, we classify the differentiated protocells into five phenotypes and encode their spontaneous organization into different spatially patterned protocell consortia using a 3-bit binary information system. We show that a predominant SDS gradient produces a diversity of differentiated phenotypes to generate complex spatially coded 2D protocell organizations. In contrast, a prevailing POM gradient decreases morphological differentiation, resulting in population homogenization. Our results improve our understanding of gradient concentration-dependent collective responses in synthetic microscale agents and provide a step to a new spatially resolved information encoding method with 3-bit binary outputs.","PeriodicalId":72566,"journal":{"name":"ChemSystemsChem","volume":"7 1","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/syst.202400064","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143118057","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Role of Kinetic Asymmetry in Chemical and Thermodynamic Coupling 动力学不对称在化学和热力学耦合中的作用

IF 3.1

ChemSystemsChem Pub Date : 2024-10-15 DOI: 10.1002/syst.202400066

R. Dean Astumian

引用次数: 0

A Pseudo-catalytic Network Motif for Thiol-based Chemical Reaction Networks 巯基化学反应网络的伪催化网络基序

IF 3.1

ChemSystemsChem Pub Date : 2024-10-14 DOI: 10.1002/syst.202400072

Ekaterina A. Zhigileva, Ilia A. Puchkin, Sergey N. Semenov

{"title":"A Pseudo-catalytic Network Motif for Thiol-based Chemical Reaction Networks","authors":"Ekaterina A. Zhigileva, Ilia A. Puchkin, Sergey N. Semenov","doi":"10.1002/syst.202400072","DOIUrl":"https://doi.org/10.1002/syst.202400072","url":null,"abstract":"The construction of chemical reaction networks (CRNs) is a formidable challenge because of their holistic and nonlinear nature. One approach to constructing CRNs involves combining fragments with distinctive properties, known as network motifs. Thiol chemistry is widely used in the construction of CRNs, with motifs available for positive and negative feedback loops. However, a simple catalytic motif has been lacking. Here, we developed a pseudo-catalytic motif using the reaction between cystamine and organic thiocyanates, which operates through a nucleophilic chain mechanism. Although similar to thiol autocatalytic systems, this reaction does not involve a doubling of the number of thiol species at any stage. The reaction is high-yielding and produces 2-amino-2-thiazoline. Its pseudo-catalytic nature manifests itself in the nearly linear relationship between the reaction rate and the amount of free thiols added at the beginning of the reaction. We demonstrated that this reaction can be regulated by external, time-dependent thiol signals and integrated into larger CRNs. We believe that this system will be a valuable addition to thiol chemistry, enabling the construction of CRNs with interesting functionalities.","PeriodicalId":72566,"journal":{"name":"ChemSystemsChem","volume":"7 2","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-10-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/syst.202400072","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143629916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-Uniform Electric Field Manipulation of Chromogenic Peptide Amphiphile Assemblies 非均匀电场操纵显色肽两亲体组装

IF 3.1

ChemSystemsChem Pub Date : 2024-10-09 DOI: 10.1002/syst.202400061

Kiara L. Lacy, Sujeung Lim, Emil M. Lundqvist, Yuyao Kuang, Harrison C. Jeong, Tayloria N. G. Adams, Herdeline Ann M. Ardoña

{"title":"Non-Uniform Electric Field Manipulation of Chromogenic Peptide Amphiphile Assemblies","authors":"Kiara L. Lacy, Sujeung Lim, Emil M. Lundqvist, Yuyao Kuang, Harrison C. Jeong, Tayloria N. G. Adams, Herdeline Ann M. Ardoña","doi":"10.1002/syst.202400061","DOIUrl":"https://doi.org/10.1002/syst.202400061","url":null,"abstract":"This work investigates the influence of dielectrophoretic forces on the structural features and the resulting aggregates of a chromogenic model system, peptide-diacetylene (D3GV-DA) amphiphiles. Here, we systematically investigate how non-uniform electric fields impact the (i) peptide-directed supramolecular assembly stage and (ii) topochemical photopolymerization stage of polydiacetylenes (PDAs) in a quadrupole-based dielectrophoresis (DEP) device, as well as the (iii) manipulation of D3GV-DA aggregates in a light-induced DEP (LiDEP) platform. The conformation-dependent chromatic phases of peptide-PDAs are utilized to probe the chain-level effect of DEP exposure after the supramolecular assembly or after the topochemical photopolymerization stage. Steady-state spectroscopic and microscopy analyses show that structural features such as the chirality and morphologies of peptidic 1-D nanostructures are mostly conserved upon DEP exposure, but applying mild, non-uniform fields at the self-assembly stage is sufficient for fine-tuning the chromatic phase ratio in peptide-PDAs and manipulating their aggregates via LiDEP. Overall, this work provides insights into how non-uniform electric fields offer a controllable approach to fine-tune or preserve the molecularly preset assembly order of DEP-responsive supramolecular or biopolymeric assemblies, as well as manipulate their aggregates using light projections, which have future implications for the precision fabrication of macromolecular systems with hierarchical structure-dependent function.","PeriodicalId":72566,"journal":{"name":"ChemSystemsChem","volume":"7 3","pages":""},"PeriodicalIF":3.1,"publicationDate":"2024-10-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://onlinelibrary.wiley.com/doi/epdf/10.1002/syst.202400061","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144091247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0