Biomedical engineering advances最新文献

{"title":"Design and development of a cost-effective portable IoT enabled multi-channel physiological signal monitoring system","authors":"Samit Hasan ,&nbsp;Tanvir Pantha,&nbsp;Muhammad Abdullah Arafat","doi":"10.1016/j.bea.2024.100124","DOIUrl":"10.1016/j.bea.2024.100124","url":null,"abstract":"<div><p>In health care, early detection of diseases is important in order to increase survival rates. Regular monitoring of vital signs is necessary for the early detection of health issues. Due to the high cost, inadequacy, and complexity of monitoring devices, it is challenging for individuals to check their vital signs at home. Consequently, a cost-effective, broadly accessible, and easy-to-use system is necessary for health monitoring. For this purpose, we developed a portable and wireless acquisition electronic device to help patients record physiologically relevant signals such as ECG, EMG, EEG, and EOG for continuous monitoring. The key components of the acquisition system are a portable device, a Wi-Fi router, a SQL server, and a graphical user interface (GUI). In this study, a cost-effective, fairly low-power Internet-of-Things (IoT)-based health monitoring system was built employing a portable device incorporating analog front ends (AFE) and the ESP 32 Wroom-32. Continuous remote monitoring and diagnostics are made possible by including IoT in the architecture. In the proposed monitoring system, the lightweight Message Queue Telemetry Transport (MQTT) protocol was used. A GUI is constructed that shows near-real-time data in a web browser and can be accessed from any operating system. The accuracy of the acquired signals was validated by comparing the individual’s ECG recorded in a remote device through the IoT cloud with a conventional biomedical certified ECG machine. The AFEs were built and evaluated based on the amplitude and bandwidth of ECG, EMG, EEG, and EOG signals. The cost and power analysis, as well as other key parameters are presented. Compared to similar existing boards, our developed system demonstrates high configurable sampling frequency, high Common Mode Rejection Ratio (CMRR) and high transmission throughput with no packet loss while costing significantly less and consuming moderate power. This makes the proposed system suited for the acquisition of multichannel physiological signals for home applications.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"7 ","pages":"Article 100124"},"PeriodicalIF":0.0,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000136/pdfft?md5=e7c65edbbf308424060687ea71e1e269&pid=1-s2.0-S2667099224000136-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141035685","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aspects of conceptualization, development, and simulation of an extracellular vesicles' bulk isolation device for cosmetic and therapeutic applications","authors":"Franck M Senda,&nbsp;Gilles C Roy","doi":"10.1016/j.bea.2024.100125","DOIUrl":"https://doi.org/10.1016/j.bea.2024.100125","url":null,"abstract":"<div><p>This paper discusses the concept and model to develop a prototype extracellular vesicle bulk-isolation device (EBID). It proposes an engineering design and potential ways to mitigate limitations in the currently available extracellular vesicle (EV) isolation techniques. A more convenient isolation technique is developed and presented to ensure production-scale isolation and purification of the specific size of EV (approximately 0.1 to 0.34 µm) from a large quantity of cell-culture media or biological fluid sources. A more appropriate design concept was established based on the chitosan-assisted precipitation of EV techniques and the membrane filtration technology-based prototype. After that, a novel theoretical model for bulk isolation of EVs was developed and presented using pressure-driven circulation and particulate flow in the EBID loop, where the EV production results from the biofluid flowing through the EBID membrane filter module (MFM). Governing equations are coupled with Darcy's law to investigate the biofluid flow behavior in the EBID MFM, therefore simulating the isolation process of EVs from their biofluids. The flow characteristics of the EV isolation process within the EBID MFM are theoretically simulated and discussed for improved isolation yield with a high degree of quality and purity.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"7 ","pages":"Article 100125"},"PeriodicalIF":0.0,"publicationDate":"2024-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000148/pdfft?md5=84dd2e142c95fd087f8ef103ffa3c9e4&pid=1-s2.0-S2667099224000148-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140950735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigating the impact of contact pressure on photoplethysmograms","authors":"Jukka-Pekka Sirkiä,&nbsp;Tuukka Panula,&nbsp;Matti Kaisti","doi":"10.1016/j.bea.2024.100123","DOIUrl":"https://doi.org/10.1016/j.bea.2024.100123","url":null,"abstract":"<div><p>Photoplethysmography (PPG) sensors are widely used to measure a variety of physiological parameters, from heart rate to clinically important peripheral oxygen saturation (SpO₂). The ease of obtaining a PPG signal simply by placing the sensor on a body site with even remotely sufficient vasculature (typically the wrist, finger, earlobe, or temple) can easily lead to overlooking the aspect of appropriate sensor contact pressure (P_c). We sought to investigate the effects of P_c from the perspective of: (i) SpO₂, (ii) pulse arrival time (PAT), and (iii) PPG features. Consequently, we developed a finger cuff device to measure multispectral (green, red, and infrared (IR)) PPG signals at different P_c levels. The SpO₂ values were found to increase, driven by the IR component, above the theoretical maximum of 100% slightly after the level of the mean arterial pressure. The maximum variation due to P_c was approximately 1.9 percentage points. PAT values calculated using the waveform feet of the red and IR channels were the most robust. PPG features were also sensitive to varying sensor P_c levels, with each feature showing a rather unique response. However, in general, a P_c between 20–30 mmHg (a slight P_c) and the level of diastolic blood pressure is likely to produce the best results on the features. Overall, the results showed that sensor P_c is a source of inaccuracy in PPG analysis and therefore should be given more consideration in device designs.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"7 ","pages":"Article 100123"},"PeriodicalIF":0.0,"publicationDate":"2024-04-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000124/pdfft?md5=c75049a57b09118f569ff1fdb1e119b5&pid=1-s2.0-S2667099224000124-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140631380","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in nanoengineered paclitaxel formulations: A comprehensive review of blood-brain barrier infiltration strategies for glioblastoma therapy","authors":"Stabak Das ,&nbsp;Prithviraj Chakraborty ,&nbsp;Debarupa Dutta Chakraborty ,&nbsp;Lila Kanta Nath","doi":"10.1016/j.bea.2024.100122","DOIUrl":"https://doi.org/10.1016/j.bea.2024.100122","url":null,"abstract":"<div><p>Glioblastoma multiform (GBM), the most occurring brain tumor comprises radiation therapy, chemotherapy, and surgery as its treatment modalities. A significant hurdle is the insufficient or impeded transport of drugs to the central nervous system (CNS), linked to the protective influence of the blood-brain barrier (BBB). Nanotechnology can help to deliver therapeutic drugs into the central nervous system (CNS) by crossing the BBB. Paclitaxel (PTX) is a broad-spectrum anticancer compound that possesses scientifically proven anticancer activity. Despite having limited applications due to partial solubility and toxicity due to cosolvent preparation, it has shown encouraging outcomes in the treatment of GBM. In these cases, nanotechnology and nanoparticles added certain advantages such as increasing drug half-life, lowering toxicity, and enhancing the permeability and retention across BBB in tumor targeting. This review article is aimed at summarizing the current state of research works on nanotechnology and nanoparticles (NPs) containing PTX in the treatment of Glioblastoma.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"7 ","pages":"Article 100122"},"PeriodicalIF":0.0,"publicationDate":"2024-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000112/pdfft?md5=814c8467bd5ceca6ef71177d92eef918&pid=1-s2.0-S2667099224000112-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140631379","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immobilization of novel synthesized phosphobetaine zwitterions on polyethersulphone (PES) hemodialysis membranes to induce hemocompatibility: Experimental, molecular docking, and ex-vivo inflammatory biomarker investigations","authors":"Ubong Eduok ,&nbsp;Shaghayegh Saadati ,&nbsp;Victoria Doan ,&nbsp;Ahmed Shoker ,&nbsp;Amira Abdelrasoul","doi":"10.1016/j.bea.2024.100120","DOIUrl":"https://doi.org/10.1016/j.bea.2024.100120","url":null,"abstract":"<div><p>Hemodialysis therapy is a crucial life-saving treatment for severe kidney conditions, particularly in cases where organ transplantation is limited. However, the use of polymeric membranes in clinical dialyzers can trigger undesirable reactions in the blood, such as complement, leukocyte, and coagulation activations. These reactions can lead to hypertension, cardiovascular diseases, and even death, due to compatibility issues. This paper presents a study on the development and application of a novel phosphobetaine zwitterion, immobilized on polyethersulphone (PES) clinical hemodialysis membranes, to improve hemocompatibility. The study also introduces a new method for immobilizing a zwitterionic PVP-phosphobetaine polymer on a PES membrane, using a polydopamine (PDA) crosslinker. The synthesized membranes were characterized, and their performance in terms of blood-protein adsorption and subsequent interaction, specifically with fibrinogen, was investigated to evaluate hemocompatibility. The selection of the phosphobetaine polymer was driven by its capacity to form an electrically neutral zwitterionic hydration layer, which serves as a protective barrier, preventing fibrinogen adsorption. Without this zwitterionic polymer, blood proteins interact with the bare membrane, initiating biological processes that lead to inflammation when exposed to uremic blood. Molecular docking studies were conducted to understand the interactions between various ligands and specific serum protein components. The phosphate and carbonyl chemical groups on the pyrrolidinone zwitterionic moiety were found to form polar interactions with specific amino acids. Exvivo investigations involving incubated coated membranes and uremic blood samples from end-stage renal disease (ESRD) patients revealed that they caused weaker complement and coagulation activation compared to bare PES membranes. In addition, the inflammatory biomarkers have been studied to shed light on their potential impact on patients' quality of life. This study contributes to our understanding of the implications of blood-protein fouling and the hemocompatibility challenges faced by blood-contacting devices used in hemodialysis for ESRD patients, who are prone to membrane-related health complications.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"7 ","pages":"Article 100120"},"PeriodicalIF":0.0,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000094/pdfft?md5=713621d04c7be794eac5d97c99cab9c0&pid=1-s2.0-S2667099224000094-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140342173","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determining Diagnostic Utility of EEG for Assessing Stroke Severity using Deep Learning Models","authors":"Shatakshi Singh ,&nbsp;Dimple Dawar ,&nbsp;Esha Mehmood ,&nbsp;Jeyaraj Durai Pandian ,&nbsp;Rajeshwar Sahonta ,&nbsp;Subhash Singla ,&nbsp;Amit Batra ,&nbsp;Cheruvu Siva Kumar ,&nbsp;Manjunatha Mahadevappa","doi":"10.1016/j.bea.2024.100121","DOIUrl":"10.1016/j.bea.2024.100121","url":null,"abstract":"<div><p>Stroke has become a leading cause of disability worldwide. Early medication and rehabilitation is the key to help post-stroke survivors recover faster. Presently, doctors rely on imaging modalities like CT/MRI for diagnosing stroke patients. The diagnosis done using these modalities can be highly subjective. Apart from this, these imaging modalities are very costly, time taking and inconvenient for the patients. So there is a need of faster, portable and an automated diagnostic system for assessing post-stroke conditions so that right measures can be taken in the right time. To cater to this need EEG comes in handy because of its portable nature. So, in this work, utility of EEG has been studied to diagnose three aspects of stroke: 1) type of stoke, 2) affected artery and 3) severity of stroke. To achieve this, one-minute resting state EEG data was used to extract 57 features. The features were ranked and selected using ranking algorithm and deep learning (DL) models were trained with supervision from information extracted using MRI data. To find out type of stroke and affected artery DWI, SWI and MRA images were used, and severity of stroke was recorded in terms of NIHSS score. Three different DL models were trained for each task i.e. type of stroke, affected artery and severity of stroke. For classifying type of stroke an accuracy of 97.74% was obtained using 37 features. For stroke severity, the model gave RMSE of 2.1955 with a high correlation value (r = 0.91). The DL model for classifying affected artery used 33 features and gave accuracy of 95.7%. It was also found that less complex time domain features and QEEG features were frequently selected out of 57 features for all the DL models. Features in delta and theta sub-bands were frequently selected along with QEEG features. The work presented here established that EEG can act as a reliable modality for faster diagnosis of stroke specifics and hence can help medical professionals in speeding the decision making process.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"7 ","pages":"Article 100121"},"PeriodicalIF":0.0,"publicationDate":"2024-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000100/pdfft?md5=530698daff608a0818105c4a51e13276&pid=1-s2.0-S2667099224000100-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140398406","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A new height contouring method for severity prediction in cam-type hip joints: 20 subject-specific cases","authors":"Trent Edward Rayment,&nbsp;Sophie Williams,&nbsp;Alison Claire Jones","doi":"10.1016/j.bea.2024.100119","DOIUrl":"https://doi.org/10.1016/j.bea.2024.100119","url":null,"abstract":"<div><p>Cam-type femoroacetabular impingement syndrome (FAIS) is characterised by a non-spherical continuation of the femoral head into the femoral head-neck junction and is associated with damage to the acetabular cartilage. Diagnostic methods based on cam shape are progressing from 2D radiographic measurements to 3D CT measures, providing greater insight. There is currently no consensus on how to describe the 3D cam lesion shape and subject-specific impingement mechanisms are difficult to determine.</p><p>A novel 3D ‘contour’ method was used to describe the proximal femur of 20 cam-type hips. Five contours, analogous to height contours on a terrain map, were used to describe the femoral head-neck junction, capturing the progressive height of the cam lesion. From that description, the <em>cam apex</em> (a subject's largest alpha angle), <em>cam extent</em> (spread around the femoral head), <em>cam location</em> (position around the femoral head) and <em>average acetabular coverage</em>, were recorded. A previously developed hip impingement model was used to apply 126 activity-based motions to each subject-specific hip shape and predict impingement occurrence and depth of incursion past the acetabular rim. Correlations between shape measures and impingement occurrence were investigated.</p><p>The two contours representing the lowest heights (close to the head best fit sphere and 1 mm greater than that) generated cam alpha angle and cam extent measurements which contained the typical clinical measures (Alpha: close to best fit 47°-98°, at 1 mm 45°–77°; Extent: close to best fit 0°–129°, at 1 mm 0°–100°). The remaining contours described the progressive height of the cam lesion up to 4 mm greater than the head radius. Impingement was predicted predominantly from the first 1 mm height of the cam, with only two subjects impinging at a cam height greater than 2 mm. Therefore, it is possible that adequate resection of the first 1 mm of cam height is the most critical in reducing a subject's impingement severity.</p><p>Impingement occurrence was positively correlated with the cam apex (ρ = 0.84 close to best fit, ρ = 0.70 at 1 mm height), the cam extent (ρ = 0.68 close to best fit, ρ = 0.80 at 1 mm height) and the acetabular coverage (ρ = 0.50, at 1 mm height). However, in line with other work on cam impingement, correlations between any single shape measure and the risk of impingement were not strong enough to be used with confidence as predictive tools. This supports the further development of modelling tools which sufficiently capture the complex shape and can generate an impingement risk metric which accounts for joint motion.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"7 ","pages":"Article 100119"},"PeriodicalIF":0.0,"publicationDate":"2024-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000082/pdfft?md5=18172cefad7057360bce393040d2aaff&pid=1-s2.0-S2667099224000082-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140195900","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative analysis of VEP signals discrimination methods based on time-frequency transformation and CNN-2D","authors":"Zineb Cheker ,&nbsp;Saad Chakkor ,&nbsp;Ahmed EL Oualkadi ,&nbsp;Mostafa Baghouri ,&nbsp;Rachid Belfkih ,&nbsp;Jalil Abdelkader El Hangouche ,&nbsp;Jawhar Laameche","doi":"10.1016/j.bea.2024.100114","DOIUrl":"https://doi.org/10.1016/j.bea.2024.100114","url":null,"abstract":"<div><p>The Visual Evoked Potential (VEP) examination is used to analyze the appropriate functioning of the optical pathways from the retina to the visual cortex. In hospitals, the diagnosis made by physicians is based mainly on reading the temporal trace and identifying the latency P100. However, after a considerable research effort, it has been confirmed that this method is subjective and relatively less reliable. In our work, we report different approaches to resolve the inadequacy of traditional classification, by studying the efficiency of VEP signal classification in a comparative approach using 3 models: Model A: STFT-CNN, Model B: CWT-CNN, and Model C: Wigner-Ville-CNN, therefore we evaluate in the same context the effectiveness of using a pre-trained 2D CNN structure. The time-frequency transformation allows us to generate two-dimensional data from one-dimensional signals to bring out the integrated features that are not valued in the temporal plot, and then exploit them for good discrimination between the two classes, in order to be able to use a CNN-2D classification architecture, taking into consideration the advantages offered by this architecture in terms of the involvement of the attribute extraction phase and its efficiency in classifying 2D data. The results provided by the different scenarios proved that the Wigner-Ville transformation combined with a pre-trained CNN architecture can be considered a good method in terms of different performance metrics, which demonstrates that it is a successful candidate for providing significant assistance to physicians in their analysis of VEP signals.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"7 ","pages":"Article 100114"},"PeriodicalIF":0.0,"publicationDate":"2024-03-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000033/pdfft?md5=1010164311d8e6313f49561b0b295232&pid=1-s2.0-S2667099224000033-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140181103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Understanding fibrosis: Mechanisms, clinical implications, current therapies, and prospects for future interventions","authors":"Jennifer C. Ku ,&nbsp;Jacob Raiten ,&nbsp;Yong Li","doi":"10.1016/j.bea.2024.100118","DOIUrl":"https://doi.org/10.1016/j.bea.2024.100118","url":null,"abstract":"<div><p>Fibrosis is a prevalent and detrimental condition associated with various diseases with a high impact on global morbidity and mortality rates. Despite its diverse causes and affected organs, common underlying mechanisms drive the development and progression of the disease. These mechanisms include an exaggerated inflammatory response, excessive activation of fibroblasts, and abnormal tissue remodeling following severe or repetitive tissue injury. Although significant advancements have been achieved to enhance our understanding of fibrosis, there is still a gap between identifying potential antifibrotic targets and successfully translating them into effective clinical interventions. Novel approaches that target specific cellular and molecular processes involved in fibrosis hold promise for reducing the pathological consequences of the disease. Understanding the pathogenesis and clinical implications of fibrotic diseases is crucial for developing effective therapeutic strategies and improving patient outcomes. In this review, we introduce the concept of fibrosis, discuss the mechanisms by which it arises, and explore existing and emerging therapeutic approaches in development.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"7 ","pages":"Article 100118"},"PeriodicalIF":0.0,"publicationDate":"2024-03-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000070/pdfft?md5=19c16f4e8297ced0f648e3f808d2b978&pid=1-s2.0-S2667099224000070-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140134131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reliable quality of R-phycoerythrin derived from Portieria hornemannii for effective antioxidant, antibacterial, and anticancer activity","authors":"Saranya Karuppannan ,&nbsp;Mani Sivakumar ,&nbsp;Balasubramani Govindasamy ,&nbsp;Santhosh Chinnaraj ,&nbsp;Viji Maluventhan ,&nbsp;Maruthupandian Arumugam","doi":"10.1016/j.bea.2024.100116","DOIUrl":"https://doi.org/10.1016/j.bea.2024.100116","url":null,"abstract":"<div><p>The phycobiliprotein is a light-harvesting fluorescent probe; Phycoerythrin is a pigment used in many industrial, and pharmaceutical applications; including fluorescence resonance energy transfer and flow cytometry. The main objective of the current study was purifying the phycoerythrin in <em>Portieria hornemannii</em>. The phycoerythrin pigment was dialyzed commonly using Q sepharose, but it is high cost. However, the alternative method is DEAE-Sephadex is cost-effective, high- yield, pure compound, and has high activity performance. Then the R-Phycoerythrin (R-PE) compound was extracted in 0.02 M of phosphate buffer (pH–7.2). R-PE investigated the FT-IR, HPLC, SDS-PAGE and including physico-chemical properties. Then the purified R-PE compound was evaluated by an antibacterial, antioxidant, and anticancer activity. The R-Phycoerythrin was showing a maximum inhibition effect of high concentration (IC₅₀value 77.98 µg/ml) against the colon cancer HT-29 cell line.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"7 ","pages":"Article 100116"},"PeriodicalIF":0.0,"publicationDate":"2024-02-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000057/pdfft?md5=002935a3085e505b6e3bda709475f7f0&pid=1-s2.0-S2667099224000057-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140162705","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}