Biomedical engineering advances最新文献

{"title":"The potential of Ti-6Al-7Nb, and design for manufacturing considerations in mitigating failure of hip implants in service","authors":"Kingsley Posiyano ,&nbsp;R.V.S. Prasad ,&nbsp;Thywill Cephas Dzogbewu ,&nbsp;Eyitayo O. Olakanmi ,&nbsp;Tshenolo P. Leso ,&nbsp;Keagisitswe Setswalo ,&nbsp;Amantle T. Sello","doi":"10.1016/j.bea.2024.100136","DOIUrl":"10.1016/j.bea.2024.100136","url":null,"abstract":"<div><div>The hip prosthesis, used to repair or recreate the diseased or damaged hip joint's articulation functionality, greatly influences the outcome of total hip arthroplasty (THA). Currently, the limited lifespan (10–15 years) of hip prostheses presents a serious challenge stemming from poor materials selection, design, as well as manufacturing techniques and this has been amplified further by the rising human life expectancy. Today's hip prostheses are predominantly made of Ti-6Al-4V alloy, which frequently fail owing to wear, modulus mismatch, corrosion, and poor osseointegration. To prolong hip implants’ useful life within the body system, it is crucial to comprehend human hip anatomy and biomechanics, investigate the modes and mechanisms of prosthesis failure, and identify mitigation measures pertaining to materials selection, prosthesis design, and production processes. From this point of view, this article firstly explores the intricate hip joint's structural anatomy in the context of biomechanics principles that influence joint movement and weight bearing. Then, hip implant failure modes and mechanisms are discussed and lastly, the failure mitigation measures are proposed. From this review, Ti-6Al-7Nb known for its excellent corrosion resistance and superior biocompatibility is considered a promising substitute for the mostly used cytotoxic Ti-6Al-4V, functionally graded porosity design mimicking the human bone to enhance mechanical and biomedical properties, more precisely osseointegration and stress shielding, and utilization of the selective laser melting technique capable of fabricating Ti-6Al-7Nb components with intricate shapes and high geometrical accuracy can play a significant role in preventing current hip implant failures.</div></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"8 ","pages":"Article 100136"},"PeriodicalIF":0.0,"publicationDate":"2024-11-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142657468","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exploring the tunable micro-/macro-structure enabled by alginate-gelatin bioinks for tissue engineering","authors":"Lucas Lemarié ,&nbsp;Jérôme Sohier ,&nbsp;Edwin-Joffrey Courtial","doi":"10.1016/j.bea.2024.100135","DOIUrl":"10.1016/j.bea.2024.100135","url":null,"abstract":"<div><div>This study explores the development of optimized alginate-gelatin (AG) bioinks for advanced 3D bioprinting applications, particularly in tissue engineering. Central to our investigation is the establishment of a method for producing AG bioinks with highly tunable viscoelastic properties and the ability to create both macro- and micro-porous scaffolds through a liquid-liquid emulsion technique applied to chemically crosslinked hydrogels and shaped by microextrusion. Our methodology encompasses a comprehensive evaluation of homogenization, pasteurization techniques, and rheological assessments to optimize the mechanical properties of AG hydrogels, ensuring their suitability for bioprinting.</div><div>The study demonstrates that dynamic homogenization and conventional pasteurization methods yield superior dissolution and sterility of the bioinks, crucial for maintaining optical quality and biological compatibility. Crosslinking optimization significantly enhanced the elasticity and reduced post-crosslinking shrinkage of the hydrogels, a key factor in achieving desired cell viability and function within the engineered tissues. The incorporation of porosity through a controlled liquid-liquid emulsion process was found to enhance cellular interactions and integration within the bioprinted constructs.</div><div>Our findings confirm that the rheological properties of bioinks play a crucial role in determining bioprintability, with temperature modulation emerging as a key tool for tailoring these characteristics. The biocompatibility and functional performance of the AG hydrogels were validated through in vitro experiments, demonstrating promising cell viability and proliferation. This research lays the groundwork for the development of advanced bioinks capable of supporting complex tissue architectures in regenerative medicine and tissue engineering. By marrying the versatility of alginate and gelatin with innovative fabrication techniques, our study advances the frontier of 3D bioprinting, paving the way for the creation of biomimetic tissues with enhanced physiological relevance and therapeutic potential.</div></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"8 ","pages":"Article 100135"},"PeriodicalIF":0.0,"publicationDate":"2024-10-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142417477","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acoustic airway clearance devices: A systematic review of experimental and numerical studies","authors":"Arife Uzundurukan ,&nbsp;Sébastien Poncet ,&nbsp;Daria Camilla Boffito ,&nbsp;Philippe Micheau","doi":"10.1016/j.bea.2024.100134","DOIUrl":"10.1016/j.bea.2024.100134","url":null,"abstract":"<div><div>The global respiratory care devices market, including acoustic airway clearance devices (ACDs), is expected to experience a compound annual growth rate of 6.10 % from 2023 to 2030. However, there are a number of inconsistencies in the categorization and working frequency range from one discipline to another one. A better understanding of the mechanisms of action of these devices is therefore of prime importance in order for physicians, physiotherapists, scientists, and engineers to remain abreast of up-to-date studies in the field and specifically on the frequency range used. In the present review, we have categorized acoustic ACDs according to their working principles while reviewing their existing shortcomings in both experimental and numerical studies, thereby paving the way for future research directions. A total of 14 different ACDs are discussed, taking into account their working principle and frequency range, and classified as follows: mechano-acoustic devices, high-frequency chest wall compression (HFCWC), and high-frequency chest wall oscillation (HFCWO) for high-frequency chest compression (HFCC) and oral high-frequency oscillation (OHFO). Existing studies highlight that ACDs with HFCWC distinguish themselves from other devices by supplying compression in a homogeneous manner, allowing the delivery of both efficient and gentle therapy up to approximately 40 Hz. Notwithstanding, a stark difference in the working frequency range across the various devices was found and identified as a literature gap. Given that this difference arises from both experimental and numerical studies between the various disciplines, the studies are further classified according to their respective objectives, methodology and outputs to help readers quickly and straightforwardly locate the articles of interest for potential future investigations. The review also brings to light the interdisciplinary nature of ACDs, whereby numerical biomedical studies can actively assist experimental studies in terms of reproducibility and reliability, creating a digital twin of the human chest and its respective components.</div></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"8 ","pages":"Article 100134"},"PeriodicalIF":0.0,"publicationDate":"2024-09-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142319728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Intelligent ultrasonic aspirator: Advancing tissue differentiation through hierarchical classification during hand-held resection","authors":"Niclas Erben ,&nbsp;Daniel Schetelig ,&nbsp;Jan Buggisch ,&nbsp;Matteo Mario Bonsanto ,&nbsp;Steffen Buschschlüter ,&nbsp;Floris Ernst","doi":"10.1016/j.bea.2024.100133","DOIUrl":"10.1016/j.bea.2024.100133","url":null,"abstract":"<div><p>Modern neurosurgery strives to maximize tumor removal while preserving healthy tissue integrity. Accurate intraoperative differentiation between tumor and healthy tissue is crucial yet challenging. Often neurosurgeons rely on their experience and haptic feedback during palpation to distinguish between tumor and healthy tissue. A commonly used hand-held tool for tissue removal during neurosurgery is the ultrasonic aspirator, which changes its electrical properties as it interacts with tissue. The goal is to equip the ultrasonic aspirator with the ability to differentiate between different types of tissue while at the same time not interfering with the surgical workflow and providing comprehensible outcomes. To this end, a hierarchical classification approach is employed as a proof of concept, enabling precise identification of tissue stiffness during resection.</p><p>The hierarchical approach is compared with the standard flat classification, commonly used in machine learning. Within the hierarchical approach, two strategies are employed: mandatory leaf-node predictions (MLNP) and non-mandatory leaf-node predictions (NMLNP). The NMLNP allows prediction to revert to a parent node when certainty is low. Data are acquired on three artificial tissue models – differing in stiffness – with an ultrasonic aspirator in a hand-held manner. The dataset comprises 1,821 data points for training and 186 for testing after balancing.</p><p>The results indicate a slight performance advantage for the hierarchical classification MLNP approach over the flat classification approach in the absence of confidence thresholds, with weighted F2-scores of 0.781 and 0.762, respectively. However, the application of confidence thresholds results in both approaches exhibiting comparable performance, with the hierarchical NMLNP approach achieving a weighted F1-score of 0.920, thereby demonstrating superior overall performance. The effects of enforcing these thresholds and excluding data with low certainty are thoroughly investigated. This work emphasizes the feasibility of tissue differentiation using a hand-held ultrasound aspirator while resecting tissue. Moreover, it highlights the capability of hierarchical classification in advancing tissue differentiation accuracy during neurosurgical procedures, which could ultimately aid surgeons and enhance the safety of intraoperative workflows.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"8 ","pages":"Article 100133"},"PeriodicalIF":0.0,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000227/pdfft?md5=af67c249a08b023db2baec85d4ad7062&pid=1-s2.0-S2667099224000227-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142162992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in the application of biomaterials in neural tissue engineering: A review","authors":"Iyad A Hammam,&nbsp;Ryan Winters,&nbsp;Zhongkui Hong","doi":"10.1016/j.bea.2024.100132","DOIUrl":"10.1016/j.bea.2024.100132","url":null,"abstract":"<div><p>Tissue engineering approaches have revolutionized the treatment of neural nerve injuries caused by disruption to axonal route or tract. Neurodegenerative diseases, traumatic brain injury (TBI), spinal cord injury (SCI), and peripheral nerve injury (PNI) change the intricate architecture, resulting in growth inhibition and loss of guidance over long distances. Neural tissue engineering aims to overcome limitations of cell-based therapeutics. Efforts are being made to create an optimal scaffold using natural, synthetic, and conductive polymers that match the biological, mechanical, and electrical properties of the native neural tissue. Combining biomaterials, cells, and biochemicals promotes axonal regrowth, facilitating functional recovery from neural nerve disorders. This review focuses on the recent advancements in neural tissue engineering technologies and their applications.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"8 ","pages":"Article 100132"},"PeriodicalIF":0.0,"publicationDate":"2024-09-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000215/pdfft?md5=cf01a0fe31738c79d83c0e8e55f875b6&pid=1-s2.0-S2667099224000215-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142162993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The possibility of using laser surface engineered titanium alloy implants as a treatment for cardiovascular diseases","authors":"Andries MTHISI,&nbsp;Abimbola Patricia Idowu POPOOLA,&nbsp;Lehlogonolo Rudolf KANYANE,&nbsp;Sadiq Abiola RAJI,&nbsp;Nicholus MALATJI","doi":"10.1016/j.bea.2024.100131","DOIUrl":"10.1016/j.bea.2024.100131","url":null,"abstract":"<div><p>Cardiovascular disorders primarily harm and shorten the lives of countless individuals worldwide. Even while surgical heart transplants and other medical procedures can help people with cardiovascular disease live longer, finding the right donor and the expense of therapy are obstacles that force patients to look for less intrusive and less expensive therapies. The use of synthetic biomaterials, such as titanium-based implants, offers an alternate path with the potential to heal and regenerate the heart. However, in most biomedical cases titanium-based implants are accompanied by surface related limitations which deter them from fulfilling their potential. Over the years, surface related shortfalls are usually addressed by fabrication of coatings exhibiting better properties using different sorts of surface modification techniques. These techniques include physical vapor depositions, plasma spraying, sol-gel and laser cladding etc. However, the exploration of employing lasers to alter the surface of cardiac based implants remains a subject that needs further research. In this work, the developments of functional coatings exhibiting good corrosion resistance and better biocompatibility are reviewed with the aim to deduce the possibility of applying such coatings on titanium based cardiovascular implants thereby alleviating burdens of this disease.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"8 ","pages":"Article 100131"},"PeriodicalIF":0.0,"publicationDate":"2024-08-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000203/pdfft?md5=0355304e9c2770f58161a345323b3053&pid=1-s2.0-S2667099224000203-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141993013","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Leaflet stresses during crimping simulations of a CoreValve Evolut Pro model using a compression loading system","authors":"Oguz C. Eren ,&nbsp;Neil W. Bressloff","doi":"10.1016/j.bea.2024.100130","DOIUrl":"https://doi.org/10.1016/j.bea.2024.100130","url":null,"abstract":"<div><p><strong>Background:</strong> Transcatheter aortic valve implantation is experiencing continued growth as an option for the treatment of aortic stenosis. With larger numbers of procedures being performed on lower risk and younger patients, there is increased scrutiny on valve durability. Leaflet stresses and potential damage have a significant role to play in this regard. Predictions of leaflet stresses have so far focused on either fluid-structure interaction simulations of blood flow through the prosthesis or, crimping simulations using a cylindrical surface. However, in reality, when a compression loading system (CLS) is used in the crimping of self-expanding valves, this could result in different stresses in the valve leaflets relative to those that might occur in crimping with a cylindrical surface.</p><p><strong>Method:</strong> A full model of a CoreValve Evolut Pro (Medtronic, Minneapolis, MN, USA) device was developed, comprising the frame, skirt and leaflets along with a representative model for the CLS as used in clinical practice. The full device was crimped to a final diameter of 18 Fr using the CLS model and the distribution and intensity of leaflet stresses was assessed. A similar assessment of leaflet stresses was also performed for crimping using radial displacement of a cylindrical surface. Comparison of the predicted leaflet stresses between the two models was undertaken, alongside a comparison of the stresses produced when dynamically loading the leaflets after deployment of the valve.</p><p><strong>Results:</strong> Both the CLS and cylinder crimping methods produced higher average and peak stresses on the leaflets compared to those produced during leaflet loading. The peak von Mises stresses for CLS crimping, cylinder crimping, and leaflet loading were 3.42 MPa, 3.92 MPa, and 1.77 MPa respectively. The leaflet folding pattern between the CLS crimping and cylinder crimping methods were different, resulting in different high stress locations on the leaflets. However, the average stress magnitude at the final crimped stage between the two crimping methods were similar.</p><p><strong>Conclusions:</strong> High fidelity simulations of crimping and expansion of a complete CoreValve Evolut Pro model using a compression loading system model have been performed, wherein the results showed that peak leaflet stresses in the crimped valve were approximately twice as high as the maximum leaflet stresses under dynamic loading. This finding has significant implications for device durability due to the high stresses and possible damage they might inflict on the leaflets. It was also found that crimping using a compression loading system versus a simpler cylindrical surface produced different folding patterns and stress distributions. However, for future studies that are not concerned with accurately capturing the leaflet folding patterns and stresses throughout the crimping process, crimping via a cylindrical surface can be used inst","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"8 ","pages":"Article 100130"},"PeriodicalIF":0.0,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000197/pdfft?md5=2910e8d5b80b4f4fd42e01abe5cd9cac&pid=1-s2.0-S2667099224000197-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141328709","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The advantages of microneedle patches compared to conventional needle-based drug delivery and biopsy devices in medicine","authors":"Alissa Reinke ,&nbsp;Eliza J Whiteside ,&nbsp;Louisa Windus ,&nbsp;Devang Desai ,&nbsp;Emma Stehr ,&nbsp;Zahra Faraji Rad","doi":"10.1016/j.bea.2024.100127","DOIUrl":"10.1016/j.bea.2024.100127","url":null,"abstract":"<div><p>Microneedle (MN) patches are composed of micron-sized needles organised in arrays and attached to the backing of a patch. The most common type is the transdermal patch, designed to uniformly penetrate the stratum corneum to reach the dermis of the skin. Recent advances in 3D printing technology have allowed the development of reproducible, efficient methods to create microneedles on a large scale, which had previously been a factor in the limited clinical uptake. In comparison to conventional drug delivery methods, MN patches have been shown to significantly reduce pain and scar generation while maintaining effective and reliable delivery of vaccines, immunotherapies, and slow-release drug therapies. The MN design has also been investigated as an alternative to conventional tissue biopsy, with positive results. Synchronous delivery of medications while monitoring biomarkers in dermal interstitial fluid (ISF) is also a promising clinical development with wide-reaching benefits. MNs are diverse in design and material composition, and with developments in fabrication technology, transdermal drug delivery has been applied to many clinical fields, including chronic illnesses such as arthritis or diabetes, cancer, immunotherapies, epidemic disease prevention and ocular treatments. While the majority of MN patch applications are still in the pre-clinical testing phase in animal models, further translation of this technology to the clinic could aid in medication and vaccine compliance, improve treatment access in rural and remote communities, improve targeted therapy applications and provide financial cost savings to the public health sector. This review evaluates the designs and applications of current transdermal MN patches for drug delivery, biomarker monitoring and diagnostic biopsies compared to conventional needle-based methods.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"8 ","pages":"Article 100127"},"PeriodicalIF":0.0,"publicationDate":"2024-06-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000161/pdfft?md5=4ce4cd73a48f27fc23576dcd277e2997&pid=1-s2.0-S2667099224000161-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141276341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Validation of upper extremity kinematics using Markerless motion capture","authors":"Robyn M. Hansen,&nbsp;Sara L. Arena,&nbsp;Robin M. Queen","doi":"10.1016/j.bea.2024.100128","DOIUrl":"https://doi.org/10.1016/j.bea.2024.100128","url":null,"abstract":"<div><p>Movement research has typically been performed using three-dimensional (3D) marker-based motion capture, which is considered the “gold-standard” for biomechanical assessment. However, limitations exist due to the lack of portability, extensive preparation for data collection, marker placement training, error due to marker movement, and possible skin irritation due to marker adhesives. There is inherent error due to motion artifact stemming from skin movement and differences in marker placement between testers. Markerless motion capture systems are emerging as a new method of kinematic assessment. These methods require little preparation and there is no need to alter participant clothing. Markerless motion capture has also been validated for the lower extremity in healthy older adults during gait. However, it has not been validated for other populations or for the assessment of upper extremity (UE) motion. Therefore, the purpose of this study was to examine differences in calculated UE kinematics between marker-based and a markerless motion capture system. Participants attended two data collection sessions. Marker-based and markerless motion capture data was collected simultaneously while participants completed the Box and Blocks test (BBT). Kinematic and spatiotemporal data from both systems was exported using identical time series to ensure the same conditions for comparisons. Intraclass Correlation Coefficients (ICCs) were calculated to determine between session reliability for both systems on range of motion and peak joint angular data to ensure movement variability was not affecting measurement consistency. ICCs and Bland Altman statistics were also calculated between the systems. Root mean square deviation (RMSD) values were determined between demeaned UE joint angles for the two systems to examine movement pattern differences. The resulting between-session ICCs for each system showed that the markerless system shared similar reliability during this task as the marker-based system, further supporting the effect of variability on between-session reliability. Between-system ICCs resulted in good (0.7&lt;ICC&lt;0.9) to excellent (ICC&gt;0.9) agreement. Bland Altman results confirmed the existence of measurement bias between the systems. RMSD values for all UE joint angles were found to be less than 6°. Overall, the results from this study support the use of markerless motion capture in clinical settings to examine upper extremity biomechanics in children.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"7 ","pages":"Article 100128"},"PeriodicalIF":0.0,"publicationDate":"2024-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000173/pdfft?md5=84d5e0cc6b0b70cec79906a42f262a8e&pid=1-s2.0-S2667099224000173-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141240762","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unveiling the intricacies of protein-protein interactions and membrane fouling: Exploring hetero-protein complex formation in binary mixtures","authors":"Majak Mapiour ,&nbsp;Amira Abdelrasoul","doi":"10.1016/j.bea.2024.100129","DOIUrl":"https://doi.org/10.1016/j.bea.2024.100129","url":null,"abstract":"<div><p>In practical applications, protein fouling studies often face limitations due to their reliance on single-protein feed experiments. It is crucial to acknowledge that interprotein interactions can significantly differ from intraprotein interactions, leading to variations in adsorption and membrane fouling behaviors. In this review, we delve into the dynamics of adsorption and membrane fouling, with a specific focus on single and binary solutions of Bovine Serum Albumin (BSA) and Lysozyme (LYZ) at or near physiological pH. These two proteins differ in terms of size, charge, and conformational stability, allowing for comparisons between small and large proteins, positively and negatively charged proteins, as well as rigid and flexible proteins. To gain further insights, we compare the findings from LYZ in single and binary solutions with those of alpha lactalbumin (α-LA), which, despite having opposite charges, shares a similar size with LYZ. The formation of BSA-LYZ heteroprotein complexes may introduce unique fouling trends in binary solutions compared to single solutions. This interplay can either enhance, reduce, or leave fouling unaffected. While studies employing the Extended DLVO (Derjaguin, Landau, Vervey, and Overbeek) theory to predict fouling in protein mixtures are limited, preliminary investigations using DLVO show promise. This approach has the potential to extend to binary and multi-protein feeds, providing valuable insights into the dynamics of fouling behavior in complex protein solutions. Considering that BSA is often used as a surrogate for Human Serum Albumin (HSA), the findings of this endeavor hold particular significance. HSA ranks the most abundant plasma proteins and, therefore, represents a crucial subject in numerous protein-related studies.</p></div>","PeriodicalId":72384,"journal":{"name":"Biomedical engineering advances","volume":"8 ","pages":"Article 100129"},"PeriodicalIF":0.0,"publicationDate":"2024-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667099224000185/pdfft?md5=bb05e156dd9c93e8b534d80b5c7487ec&pid=1-s2.0-S2667099224000185-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141325711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}