Biomaterials and biosystems最新文献

{"title":"Application of bioengineered elastin-like polypeptide-based system for targeted gene delivery in tumor cells","authors":"Aena Yi ,&nbsp;Dahye Sim ,&nbsp;Seon-Boon Lee,&nbsp;Vijaya Sarangthem,&nbsp;Rang-Woon Park","doi":"10.1016/j.bbiosy.2022.100050","DOIUrl":"10.1016/j.bbiosy.2022.100050","url":null,"abstract":"<div><p>Successful gene delivery depends on the entry of negatively charged DNAs and oligonucleotides across the various barriers of the tumor cells and localization into the nucleus for its transcription and protein translation. Here, we have reported a thermal responsive self-assemble and highly biocompatible, targeted ELP-based gene delivery system. These systems consist of cell-penetrating peptides, Tat and single or multiple repeats of IL-4 receptor targeting peptide AP-1 along the backbone of ELP. Cell-penetrating peptides were introduced for nuclear localization of genes of interest, AP-1 for targeting IL-4R highly expressed tumor cells and ELP for stable condensation favoring protection of nucleic acids. The designed multidomain fusion ELPs referred to as Tat-ELP, Tat-A₁E₂₈ and Tat-A₄V₄₈ were employed to generate formulation with pEGFP-N1. Profound formulation of stable complexes occurred at different molar ratios owing to electrostatic interactions of positively charged amino acids in polymers with negatively charged nucleic acids. Among the complexes, Tat-A₄V₄₈ containing four copies of AP-1 showed maximum complexation with pEGFP-N1 in lower molar ratio. The polymer-pEGFP complexes were further analyzed for its transfection efficiency in different cancer cell lines. Both the targeted polymers, Tat-A₄V₄₈ and Tat-A₁E₂₈ upon transfection displayed significant EGFP-expression with low toxicity in different cancer cells. Therefore, both Tat-A₄V₄₈ and Tat-A₁E₂₈ can be considered as novel transfection system for successful gene delivery with therapeutic applications.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"6 ","pages":"Article 100050"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/b0/04/main.PMC9934475.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10772787","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D printed scaffolds: Challenges toward developing relevant cellular in vitro models","authors":"Beatriz Molina-Martínez ,&nbsp;Luis M. Liz-Marzán","doi":"10.1016/j.bbiosy.2022.100044","DOIUrl":"https://doi.org/10.1016/j.bbiosy.2022.100044","url":null,"abstract":"<div><p>Three-dimensional (3D) cell culture technology has rapidly emerged, as a result of the increasing demand for improved <em>in vitro</em> systems that better resemble human physiology. Promising microphysiological systems have been fabricated by combining complex 3D culture with 3D-printing technologies. These models overperform existing <em>in vitro</em> systems regarding potential for biofabrication and predictive power. However, most systems under development do not ultimately find a long-term application. We provide herein an overview of the challenges to be considered when developing 3D <em>in vitro</em> systems by means of printed scaffolds, as well as some of the limitations of existing models.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"6 ","pages":"Article 100044"},"PeriodicalIF":0.0,"publicationDate":"2022-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S266653442200006X/pdfft?md5=a809c3f30777406e491f645fa0b276b0&pid=1-s2.0-S266653442200006X-main.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"91685132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Amorphous calcium phosphate, the lack of order is an abundance of possibilities","authors":"Lorenzo Degli Esposti,&nbsp;Michele Iafisco","doi":"10.1016/j.bbiosy.2021.100037","DOIUrl":"10.1016/j.bbiosy.2021.100037","url":null,"abstract":"<div><p>For almost three decades from its discovery, amorphous calcium phosphate (ACP) was not considered a suitable biomaterial due to its structural instability. Thanks to its unique properties in respect to crystalline calcium phosphate phases, nowadays ACP is used in promising devices for hard tissue regeneration. Here we have highlighted the features of ACP that were harnessed to create excellent biomaterials for dental remineralization, self-setting bone cements, drug delivery, and coatings of prostheses. Its current limitations as well as future perspectives of development were concisely described. Despite more research works are needed, we envisage that the future of ACP is bright.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"5 ","pages":"Article 100037"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9934462/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10762327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of decellularization protocol for caprine small intestine submucosa as a biomaterial","authors":"Hemant Singh ,&nbsp;Shiv Dutt Purohit ,&nbsp;Rakesh Bhaskar ,&nbsp;Indu Yadav ,&nbsp;Mukesh Kumar Gupta ,&nbsp;Narayan Chandra Mishra","doi":"10.1016/j.bbiosy.2021.100035","DOIUrl":"10.1016/j.bbiosy.2021.100035","url":null,"abstract":"<div><p>Decellularized animal tissues have been proven to be promising biomaterials for various tissue engineering (TE) applications. Among various animal tissues, small intestine submucosa (SIS) has gained attention of many researchers due to its easy availability from the abattoir waste, excellent physicochemical and biological characteristics of a good biomaterial. In this study, Caprine SIS was decellularized to get decellularized caprine SIS (DG-SIS). For decellularization, several physical, chemical and enzymatic protocols have been described in the literature. To optimize the decellularization of caprine SIS, several decellularization protocol (DP), including an in-house developed by us, had been attempted, and effect of the different DPs on the obtained DG-SIS were assessed in terms of decellularization, physiochemical and biological properties. All the DPs differ in terms of decellularization, but three DPs where ionic detergent like sodium dodecyl sulphate (SDS) has been used, largely affect the native composition (e.g. glycosaminoglycans (GAGs)), biological properties and other physiochemical properties of the G-SIS as compared to the DP that uses hypertonic solution of potassium iodide (KI) and non-ionic detergent (TritonX-100). The obtained DG-SISs were fibrous, hemocompatible, biocompatible, hydrophilic, biodegradable and exhibited significant antibacterial activity. Therefore, the DG-SIS will be a prospective biomaterial for TE applications.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"5 ","pages":"Article 100035"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/75/23/main.PMC9934478.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10770004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Biomaterials enhancing performance of cell and nucleic-acid therapies: An opportunity in the brain","authors":"Christopher Lally ,&nbsp;Kieran Joyce ,&nbsp;Abhay Pandit","doi":"10.1016/j.bbiosy.2021.100036","DOIUrl":"10.1016/j.bbiosy.2021.100036","url":null,"abstract":"<div><p>The brain has limited innate ability to promote repair, regeneration and functional recovery following injury, disease, or developmental disorder. Although cell and gene therapies have significant potential in the brain, no single treatment is likely to succeed in isolation. Here we discuss the current state of the art in cell and nucleic-acid-based neurotherapeutics and argue for the development of combination therapies that use biomaterials to help overcome the current limitations of cell and gene therapies alone.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"5 ","pages":"Article 100036"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9934481/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10762328","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Spacer length and serum protein adsorption affect active targeting of trastuzumab-modified nanoparticles","authors":"Christina Barth ,&nbsp;Hendrik Spreen ,&nbsp;Dennis Mulac ,&nbsp;Lucas Keuter ,&nbsp;Matthias Behrens ,&nbsp;Hans-Ulrich Humpf ,&nbsp;Klaus Langer","doi":"10.1016/j.bbiosy.2021.100032","DOIUrl":"10.1016/j.bbiosy.2021.100032","url":null,"abstract":"<div><p>Receptor-mediated active targeting of nanocarriers is a widely investigated approach to specifically address cancerous cells and tissues in the human body. The idea is to use these formulations as drug carriers with enhanced specificity and therefore reduced systemic side effects. Until today a big obstacle to reach this goal remains the adsorption of serum proteins to the nanocarrier's surface after contact with biological fluids. In this context different nanoparticle characteristics could be beneficial for effective active targeting after formation of a protein corona which need to be identified. In this study trastuzumab was used as an active targeting ligand which was covalently attached to human serum albumin nanoparticles. For coupling reaction different molecular weight spacers were used and resulting physicochemical nanoparticle characteristics were evaluated. The <em>in vitro</em> cell association of the different nanoparticle formulations was tested in cell culture experiments with or without fetal bovine serum. For specific receptor-mediated cell interaction SK-BR-3 breast cancer cells with human epidermal growth factor receptor 2 (HER2) overexpression were used. MCF-7 breast cancer cells with normal HER2 expression served as control. Furthermore, serum protein adsorption on respective nanoparticles was characterized. The qualitative and quantitative composition of the protein corona was analyzed by SDS-PAGE and LC-MS/MS and the influence of protein adsorption on active targeting capability was determined.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"5 ","pages":"Article 100032"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d5/4c/main.PMC9934468.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10762331","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Magnetic CuFe2O4 with intrinsic protease-like activity inhibited cancer cell proliferation and migration through mediating intracellular proteins","authors":"Daomei Chen ,&nbsp;Liang Jiang ,&nbsp;Tao Lei ,&nbsp;Guo Xiao ,&nbsp;Yuanfeng Wang ,&nbsp;Xiaoqiong Zuo ,&nbsp;Bin Li ,&nbsp;Lingli Li ,&nbsp;Jiaqiang Wang","doi":"10.1016/j.bbiosy.2021.100038","DOIUrl":"10.1016/j.bbiosy.2021.100038","url":null,"abstract":"<div><p>Protease has been widely used in biological and industrial fields. Developing efficient artificial enzyme mimics remains a major technical challenge due to the high stability of peptide bonds. Nanoenzymes with high stability, high activity and low cost, provided new opportunities to break through natural enzyme inherent limitations. However, compared with many nanomaterials with inherent peroxidase activity, the intrinsic mimic proteases properties of magnetic nanomaterials were seldom explored, let alone the interaction between magnetic nanomaterials and cellular proteins. Herein, we reported for the first time that magnetic CuFe₂O₄ possesses inherent protease activity to hydrolyze bovine serum albumin (BSA) and casein under physiological conditions, and the CuFe₂O₄ is more resistant to high temperature than the natural trypsin. It also exhibited significantly higher catalytic efficiency than other copper nanomaterials and can be recycled for many times. Protease participated in pathophysiological processes and all stages of tumor progression. Interesting, CuFe₂O₄ exhibited anti-proliferative effect on A549, SKOV3, HT-29, BABL-3T3 and HUVEC cells, as well as it was particularly sensitive against SKOV3 cells. CuFe₂O₄ was about 30 times more effective than conventional chemotherapy drugs oxaliplatin and artesunate against SKOV3 cells. In addition, CuFe₂O₄ also mediated the expression of intracellular proteins, such as MMP-2, MMP-9, F-actin, and NF-<em>k</em>B, which may be associated with global protein hydrolysis by CuFe₂O₄, leading to inhibition of cell migration. The merits of the high magnetic properties, good protease-mimic and antitumor activities make CuFe₂O₄ nanoparticles very prospective candidates for many applications such as proteomics and biotechnology.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"5 ","pages":"Article 100038"},"PeriodicalIF":0.0,"publicationDate":"2022-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e1/a9/main.PMC9934488.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10770005","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A review of strategies for development of tissue engineered meniscal implants","authors":"George J. Klarmann ,&nbsp;Joel Gaston ,&nbsp;Vincent B. Ho","doi":"10.1016/j.bbiosy.2021.100026","DOIUrl":"10.1016/j.bbiosy.2021.100026","url":null,"abstract":"<div><p>The meniscus is a key stabilizing tissue of the knee that facilitates proper tracking and movement of the knee joint and absorbs stresses related to physical activity. This review article describes the biology, structure, and functions of the human knee meniscus, common tears and repair approaches, and current research and development approaches using modern methods to fabricate a scaffold or tissue engineered meniscal replacement. Meniscal tears are quite common, often resulting from sports or physical training, though injury can result without specific contact during normal physical activity such as bending or squatting. Meniscal injuries often require surgical intervention to repair, restore basic functionality and relieve pain, and severe damage may warrant reconstruction using allograft transplants or commercial implant devices. Ongoing research is attempting to develop alternative scaffold and tissue engineered devices using modern fabrication techniques including three-dimensional (3D) printing which can fabricate a patient-specific meniscus replacement. An ideal meniscal substitute should have mechanical properties that are close to that of natural human meniscus, and also be easily adapted for surgical procedures and fixation. A better understanding of the organization and structure of the meniscus as well as its potential points of failure will lead to improved design approaches to generate a suitable and functional replacement.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"4 ","pages":"Article 100026"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbiosy.2021.100026","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9336302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Third body damage and wear in arthroplasty bearing materials: A review of laboratory methods","authors":"Raelene M Cowie,&nbsp;Louise M Jennings","doi":"10.1016/j.bbiosy.2021.100028","DOIUrl":"10.1016/j.bbiosy.2021.100028","url":null,"abstract":"<div><p>Third body wear of arthroplasty bearing materials can occur when hard particles such as bone, bone cement or metal particles become trapped between the articulating surfaces. This can accelerate overall implant wear, potentially leading to early failure. With the development of novel bearing materials and coatings, there is a need to develop and standardise test methods which reflect third body damage seen on retrieved implants. Many different protocols and approaches have been developed to replicate third body wear in the laboratory but there is currently no consensus as to the optimal method for simulating this wear mode, hence the need to better understand existing methods. The aim of this study was to review published methods for experimental simulation of third body wear of arthroplasty bearing materials, to discuss the advantages and limitations of different approaches, the variables to be considered when designing a method and to highlight gaps in the current literature. The methods were divided into those which introduced abrasive particles into the articulating surfaces of the joint and those whereby third body damage is created directly to the articulating surfaces. However, it was found that there are a number of parameters, for example the influence of particle size on wear, which are not yet fully understood. The study concluded that the chosen method or combination of methods used should primarily be informed by the research question to be answered and risk analysis of the device.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"4 ","pages":"Article 100028"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/j.bbiosy.2021.100028","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9336301","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Collagen type II: From biosynthesis to advanced biomaterials for cartilage engineering","authors":"Z Wu ,&nbsp;SH Korntner ,&nbsp;AM Mullen ,&nbsp;DI Zeugolis","doi":"10.1016/j.bbiosy.2021.100030","DOIUrl":"10.1016/j.bbiosy.2021.100030","url":null,"abstract":"<div><p>Collagen type II is the major constituent of cartilage tissue. Yet, cartilage engineering approaches are primarily based on collagen type I devices that are associated with suboptimal functional therapeutic outcomes. Herein, we briefly describe cartilage's development and cellular and extracellular composition and organisation. We also provide an overview of collagen type II biosynthesis and purification protocols from tissues of terrestrial and marine species and recombinant systems. We then advocate the use of collagen type II as a building block in cartilage engineering approaches, based on safety, efficiency and efficacy data that have been derived over the years from numerous <em>in vitro</em> and <em>in vivo</em> studies.</p></div>","PeriodicalId":72379,"journal":{"name":"Biomaterials and biosystems","volume":"4 ","pages":"Article 100030"},"PeriodicalIF":0.0,"publicationDate":"2021-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9934443/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9336299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}