Biomaterial investigations in dentistry最新文献

{"title":"Cytotoxicity evaluation of different clear aligner systems on human primary gingival fibroblasts: an in vitro study.","authors":"Rituparna Pronajit Das, Parag Vishnu Gangurde, Alok Ranjan, Shashank Sharad Gaikwad, Hitesh Ramdas Sawant, Manav Karia","doi":"10.2340/biid.v13.46006","DOIUrl":"10.2340/biid.v13.46006","url":null,"abstract":"<p><strong>Background: </strong>Clear aligners, widely adopted in modern orthodontics, are designed to stay in constant contact with the oral mucosa for extended durations. Persistent concerns exist about the possible leaching of cytotoxic agents like bisphenol-A from these materials, which have heightened the need for rigorous biocompatibility evaluation. Among various assays, the MTT (3-(4,5-dimethylthiazol-2-yl)-2,5- diphenyltetrazolium bromide) assay is a reliable in vitro method, which assesses metabolic activity and cell viability, making it a gold standard for cytotoxicity screening.</p><p><strong>Objective: </strong>The objective was to assess and contrast the potential cell-damaging effects of three different clear aligner brands - Invisalign^®, Smile^® Aligners, and Illusion^® Aligners using the MTT assay on human gingival fibroblasts (HGFs).</p><p><strong>Methods: </strong>Aligner pellets were immersed in normal saline for 30 days at 37°C. Eluents were diluted to 5, 10, and 20% concentrations. HGFs were exposed to these eluents for 48 hours, and cytotoxicity was assessed via MTT assay. To assess the survival rate of the cells, light absorbance was recorded at a wavelength of 570 nanometers.</p><p><strong>Results: </strong>Invisalign^® showed the highest biocompatibility (97-98% viability across concentrations), followed by Smile^® Aligners (94-99%). Illusion^® Aligners exhibited significant cytotoxicity (80-92% viability), with a dose-dependent decline (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>Material composition critically influences cytotoxicity. Invisalign^® and Smile^® Aligners are a safe options, while Illusion^® Aligners require further refinement for clinical safety. These results highlight the need for transparency in material composition and standardization in manufacturing protocols for all aligner systems to ensure patient safety.</p>","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"13 ","pages":"46006"},"PeriodicalIF":0.0,"publicationDate":"2026-05-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13176899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147965619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of output intensity of light curing unit on coronal tooth discoloration induced by silicate calcium-based materials: an ex vivo study.","authors":"Fatemeh Farshad, Noushin Shokouhinejad, Parham Pedram, Mohammad Javad KharaziFard, Elham Ahmadi","doi":"10.2340/biid.v13.45896","DOIUrl":"10.2340/biid.v13.45896","url":null,"abstract":"<p><strong>Objective: </strong>Clinicians have various curing options due to new light-cure devices with different intensities. The present study aimed to evaluate the effect of two light-curing intensities (1,200 mW/cm² vs. 3,000 mW/cm²) on coronal discoloration induced by two calcium silicate-based cements (OrthoMTA and RetroMTA) in an endodontically treated bovine tooth model.</p><p><strong>Materials and methods: </strong>Forty single-rooted bovine incisors were prepared, and their canals were filled with blood-soaked foam to the cemento-enamel junction. The samples were randomly divided into four groups (<i>n</i> = 10 per group) based on the cement used (OrthoMTA or RetroMTA) and the subsequent light-curing intensity. For all groups, a 2-mm layer of the assigned cement was placed. This was covered by 1 mm of resin-modified glass ionomer (RMGI), a bonding agent, and 2 mm of composite. In two groups, all light-curing steps (RMGI, bonding agent, composite) were performed at 1,200 mW/cm². In the other two groups, curing was performed at 3,000 mW/cm². All samples underwent 5,000 cycles of thermocycling. Color was assessed with a spectrophotometer immediately after composite placement and after aging. Color change (ΔE) and lightness change (ΔL) were calculated.</p><p><strong>Results: </strong>All groups exhibited ΔE values exceeding the clinically acceptable threshold (ΔE > 3.3) and demonstrated a decrease in ΔL after aging. Statistical analysis (two-way ANOVA, α = 0.05) revealed no significant difference in ΔE or ΔL between samples cured with high-intensity (3,000 mW/cm²) or low-intensity (1,200 mW/cm²) light, regardless of the cement type used (<i>p</i> > 0.05).</p><p><strong>Conclusions: </strong>Within the limitations of this study, the same amount of energy produced by high- or low-intensity light-cures (1,200 or 3,000 mW/cm²), when delivering the same total energy, did not result in a statistically significant difference in coronal discoloration caused by OrthoMTA or RetroMTA.</p>","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"13 ","pages":"45896"},"PeriodicalIF":0.0,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13163624/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147936176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of red propolis addition on the color stability and surface roughness of glass ionomer cements.","authors":"Giovanna Kelly Melo de Lima Sampaio, Pedro Henrique Sette-de-Souza, Basílio Rodrigues Vieira, Gêisa Aiane de Morais Sampaio","doi":"10.2340/biid.v13.45991","DOIUrl":"10.2340/biid.v13.45991","url":null,"abstract":"<p><strong>Objective: </strong>The present study aimed to evaluate the surface roughness (SR) and color stability of conventional restorative glass ionomer cements (GICs) modified with the addition of red propolis ethanolic extract (RPEE) as an antimicrobial agent.</p><p><strong>Methodology: </strong>Four GICs (Riva, Maxxion, Vidrion, and Ketac) were used with the addition of RPEE at concentrations of 11% and 20%. For the control groups, the GICs were manipulated according to the manufacturers' instructions. SR was assessed using a surface profilometer. For the color variation analysis, the specimens' colors were measured in the CIE L<i>a</i>b* color space using a spectrophotometer. The Kruskal-Wallis test, followed by Dunn's test, was used to evaluate statistical significance.</p><p><strong>Results: </strong>The addition of RPEE did not negatively affect the SR of Riva, Maxxion, and Ketac GICs (<i>p</i> = 0.796, <i>p</i> = 0.111, and <i>p</i> = 0.858, respectively). By contrast, a significant reduction in SR was observed in Vidrion GIC with the addition of RPEE (<i>p</i> = 0.009). When comparing the different GIC brands, Riva and Ketac exhibited the lowest SR, with significant differences relative to Vidrion (<i>p</i> = 0.008) and Maxxion (<i>p</i> = 0.006). Both RPEE concentrations (11% and 20%) caused major color changes in all GICs tested, with no statistical differences between the two concentrations (<i>p</i> = 1.000), nor among the different GIC brands (<i>p</i> = 0.071).</p><p><strong>Conclusion: </strong>The incorporation of RPEE into conventional GIC did not increase SR, though in the case of Vidrion cement, it resulted in a significant reduction of this parameter. Conversely, the addition of red propolis at both 11% and 20% concentrations caused significant and clinically unacceptable color changes in all tested cements, regardless of brand or concentration.</p>","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"13 ","pages":"45991"},"PeriodicalIF":0.0,"publicationDate":"2026-05-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13163625/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147935649","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative evaluation of <i>Calotropis gigantea</i> latex and sodium hypochlorite as tissue-dissolving agents - an <i>in vitro</i> study on concentration and time-dependent proficiency.","authors":"Megavarnan Ravi, Praveena Annadurai, Ramlakhan Gupta","doi":"10.2340/biid.v13.45992","DOIUrl":"10.2340/biid.v13.45992","url":null,"abstract":"<p><strong>Background: </strong>Complete removal of organic tissue and microorganisms from the root canal system is essential for successful endodontic therapy. Sodium hypochlorite (NaOCl) remains the gold standard irrigant for its tissue-dissolving and antimicrobial properties, but its cytotoxicity and adverse effects have encouraged exploration of safer herbal alternatives. <i>Calotropis gigantea</i> (milkweed) latex contains proteolytic enzymes such as calotropin and calotoxin, which may offer effective organic tissue dissolution with improved biocompatibility.</p><p><strong>Aim: </strong>To evaluate and compare the tissue-dissolving potential of <i>C. gigantea</i> latex at two concentrations (5 and 10%) with 5.25% NaOCl at different exposure times.</p><p><strong>Methodology: </strong>An <i>in vitro</i> experimental study was conducted on pulp tissues extracted from freshly removed human teeth (<i>n</i> = 30). Samples were equally divided into three groups: 5% <i>C. gigantea</i> latex, 10% <i>C. gigantea</i> latex, and 5.25% NaOCl. Tissue samples were immersed for 10 minutes and 15 minutes, and weight reduction was measured using an analytical balance. Data were analysed using one-way analysis of variance and Tukey's <i>post hoc</i> test with a significance level of <i>p</i> < 0.05.</p><p><strong>Results: </strong>The mean initial tissue weights were comparable among groups (<i>p</i> = 0.656). After 10 minutes, significant differences in weight reduction were observed (<i>p</i> < 0.001). Tukey's <i>post hoc</i> test showed that 10% <i>C. gigantea</i> latex dissolved significantly more tissue than both 5% latex and NaOCl (<i>p</i> < 0.001). After 15 minutes, this difference persisted (<i>p</i> < 0.001). The 10% latex achieved the highest mean weight loss at both intervals, while no significant difference was observed between 5% latex and NaOCl.</p><p><strong>Conclusion: </strong>The 10% <i>C. gigantea</i> latex solution showed higher tissue-dissolving ability compared to 5.25% NaOCl under static <i>in vitro</i> conditions. Clinical applicability requires further evaluation of activation methods, cytotoxicity, dentin interaction, and antimicrobial efficacy.</p>","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"13 ","pages":"45992"},"PeriodicalIF":0.0,"publicationDate":"2026-05-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13158728/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147936252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Surface roughness and microhardness of 3D printed denture base resins when printed with different printers.","authors":"Faisal D Al-Qarni","doi":"10.2340/biid.v13.45751","DOIUrl":"10.2340/biid.v13.45751","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the influence of using different printers on surface roughness and hardness of different 3D printed dentures.</p><p><strong>Methods: </strong>Three denture base resins (ASIGA, NextDent, and FormLabs) were printed with one of two printers: ASIGA or NextDent. Surface roughness was measured with a non-contact profilometer, while a surface hardness tester was used to measure Vicker's hardness values. One-way and Two-way analysis of variance (ANOVA), and <i>t</i>-test were used to compare means with a significance level set at 0.05.</p><p><strong>Results: </strong>FormLabs and NextDent specimens showed different hardness values when different printers were used (<i>p</i> < 0.001), while ASIGA specimens had no difference (<i>p</i> = 0.072). Roughness values were similar with NextDent specimens printed with different printers (<i>p</i> = 0.053), while ASIGA and FormLabs specimens' roughness values were significantly influenced by the printer used (<i>p</i> < 0.001). When printed with ASIGA, ASIGA and NextDent specimens had similar hardness values, which were higher than FormLabs specimens. When NextDent printer was used, ASIGA specimens showed superior hardness values compared to NextDent and FormLabs. When comparing surface roughness of different resin specimens printed with the same printer, differences were observed among all specimens (<i>p</i> < 0.001).</p><p><strong>Conclusions: </strong>The type and brand of the printer used can affect surface properties of printed dentures. When the same printer was used, surface roughness and hardness were significantly different among all resin materials. The choice of resin material and printer combination affects both surface roughness and microhardness.</p><p><strong>Clinical significance: </strong>The choice of resin material and printer type may significantly impact the microhardness and surface roughness of 3D printed dentures. Careful material and printer selection is essential to ensure optimal clinical performance and longevity of prostheses.</p>","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"13 ","pages":"45751"},"PeriodicalIF":0.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13158727/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147935664","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Role of cold atmospheric plasma alone or combined with conventional surface treatments on shear bond strength of 3Y-TZP and 5YSZ ceramics bonded to dentin.","authors":"Ahmad Abdulkareem Alnazzawi, Mohamed F Aldamaty, Mohammed H AbdElaziz, Ahmed Yaseen Alqutaibi, Ahmed E Farghal, Jamal Qernas Almarashi, Abdel-Aleam H Mohamed, Muhammad Sohail Zafar","doi":"10.2340/biid.v13.45563","DOIUrl":"10.2340/biid.v13.45563","url":null,"abstract":"<p><p>This study evaluated the impact of different surface treatments - cold atmospheric plasma (CAP), airborne particle abrasion (APA), hydrofluoric acid (HF), and their combinations - on the bond strength of two zirconia types (3Y-TZP, 5YSZ) to dentin using self-adhesive resin cement, with or without primer. Two hundred zirconia and dentin specimens underwent surface treatment, cementation, thermocyclic aging, and shear bond strength (SBS) testing. Results showed APA and CAP significantly enhanced SBS (<i>P</i> < 0.001), while HF had no effect. Combined treatments (APA+CAP, CAP+HF) slightly reduced SBS. Primer application improved SBS across all groups. APA or CAP with a 10-MDP primer achieved the highest SBS values. CAP is a viable alternative to APA, though APA remains cost effective. HF is not recommended for surface treatment of zirconia. CAP exhibited a slightly higher, yet statistically comparable, SBS between zirconia and dentin when compared with APA. Nonetheless, APA may still represent the simplest and most cost-effective technique available to date. The combination of APA and CAP might not be recommended, as it showed a slight reduction in SBS of the tested specimens and inefficiency in terms of time and energy. Hydrofluoric acid showed no impact on SBS when combined with CAP. Both 3Y-TZP and 5YSZ can provide adequate clinical bond strength with the proper surface treatment and adhesive protocol.</p>","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"13 ","pages":"45563"},"PeriodicalIF":0.0,"publicationDate":"2026-05-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13154724/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147870578","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mineralization effect of ion-releasing fiber-reinforced composite in teeth with molar-incisor hypomineralization.","authors":"Lippo Lassila, Battsetseg Tseveenjav, Janna Waltimo-Sirén, Pekka Vallittu, Sufyan Garoushi","doi":"10.2340/biid.v13.45993","DOIUrl":"10.2340/biid.v13.45993","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the long-term mineralizing effects of an experimental ion-releasing, short fiber-reinforced flowable composite (SFC-active) applied to human teeth with molar-incisor hypomineralization (MIH).</p><p><strong>Methods: </strong>A total of 16 first permanent molars, extracted due to MIH, received two occlusal restorations each. All cavities were acid-etched for 15 seconds before applying the restorative materials. One of the cavities in each tooth was restored with a commercial conventional particulate-filled composite (PFC; G-aenial Universal Injectable) after placement of the SFC-active liner. The other cavities were restored without the liner, using PFC alone (<i>n</i> = 8) or resin-modified glass ionomer cement (RMGIC; Fuji II LC) alone (<i>n</i> = 8). The teeth were stored in simulated body fluid at 37°C for 30 months. The mineralization effect was assessed at three regions (coronal, middle, and apical) under the restorations using micro-computed tomography (CT) (dentin density), micro-indentation (dentin hardness) and scanning electron microscopy/energy-dispersive spectroscopy (microstructure and calcium-to-phosphorus [Ca/P] ratio) analyses.</p><p><strong>Results: </strong>Micro-CT analyses revealed no statistically significant differences (<i>p</i> > 0.05) in dentin mineral density between the restorative materials at any of the three regions beneath the restorations. At the coronal region of interface, dentin hardness was higher with SFC-active than with PFC, but lower than with RMGIC (<i>p</i> < 0.05). The Ca/P ratios of dentin varied beneath the different restorations, ranging from 1.49 to 1.60, with the highest ratios observed at the coronal region of the interface with SFC-active. Strontium and fluorine were detected in the dentin adjacent to the RMGIC restorations.</p><p><strong>Conclusion: </strong>SFC-active demonstrated a positive mineralizing effect on dentin, reflected by higher hardness and Ca/P ratios at the coronal region of the interface. These findings indicate that SFC-active is a promising restorative material for the management of MIH-affected teeth.</p>","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"13 ","pages":"45993"},"PeriodicalIF":0.0,"publicationDate":"2026-04-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13139956/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147846600","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of mineral precipitation, elemental release, pH change and cytotoxicity of calcium-silicate cements and an experimental resin-modified glass ionomer cement containing bioactive glass.","authors":"Wisitsin Potiprapanpong, Parichart Naruphontjirakul, Naruporn Monmaturapoj, Siriporn Tanodekaew, Somruethai Channasanon, Arnit Toneluck, Somying Patntirapong, Piyaphong Panpisut","doi":"10.2340/biid.v13.45796","DOIUrl":"10.2340/biid.v13.45796","url":null,"abstract":"<p><strong>Introduction: </strong>Resin-modified glass ionomer cements (RMGICs) exhibit lower remineralising potential than calcium-silicate (Ca-Si) cements. This study aimed to prepare an experimental RMGIC incorporating Sr/F-bioactive glass nanoparticles (EXP) to enhance remineralisation on demineralised dentine. The experimental material was compared with commercial Ca-Si cements (RetroMTA [MTA], Biodentine [BDT], Theracal LC [TC]) and a commercial RMGIC (Vitrebond [VB]).</p><p><strong>Materials and methods: </strong>Demineralised dentine specimens were attached to discs of each material and immersed in simulated body fluid for up to 4 weeks. Mineral precipitation was assessed using an Attenuated Total Reflection - Fourier Transform Infrared Spectroscopy (ATR-FTIR) and scanning electron microscopy-energy-dispersive X-ray spectroscopy analyses. Remineralisation was quantified as the mineral-to-collagen ratio, calculated from the phosphate FTIR peak height at 1024 cm^-1 relative to the amide I peak at 1636 cm^-1 (<i>n</i> = 5). Changes in pH and elemental release (Na, Al, Si, P, Ca and Sr) from materials immersed in deionised water over 4 weeks were also evaluated (<i>n</i> = 3). Indirect cytotoxicity of material extracts on human dental pulp stem cells was assessed using the 3-(4,5 dimethylthiazol-2-yl)-2,5-di-phenyltetrazolium bromide assay (MTT) assay (n=4). Statistical analysis was performed using one-way analysis of variance (ANOVA) followed by Tukey's Honestly Significant Difference test.</p><p><strong>Results: </strong>The highest increase in the mineral-to-collagen ratio was observed with MTA (78%), which was significantly higher than that of VB (-28%) and EXP (-33%). Scanning electron microscopy analysis demonstrated mineral precipitation with MTA, BDT and TC but not with VB or EXP. Additionally, MTA, BDT and TC produced the highest alkalinisation of the storage solution (pH~12). EXP exhibited the release of multiple ions (Na, Al, Si, P, Ca and Sr). Although MTA showed the highest cell viability, all materials demonstrated cell viability exceeding 70%.</p><p><strong>Conclusion: </strong>The experimental RMGIC containing Sr/F-bioactive glass nanoparticles exhibited inferior remineralising potential compared with Ca-Si cements. However, it promoted the release of multiple essential ions.</p>","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"13 ","pages":"45796"},"PeriodicalIF":0.0,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13139827/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147846533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of long soaking in Kawa Daun beverage on discoloration of nanofilled composite resin.","authors":"Deli Mona, Syifa Mahdiyah","doi":"10.2340/biid.v13.45575","DOIUrl":"10.2340/biid.v13.45575","url":null,"abstract":"<p><strong>Objective: </strong>This study investigated the effect of prolonged soaking in the Kawa Daun beverage on the discoloration of nanofilled composite resin.</p><p><strong>Materials and methods: </strong>Thirty disk-shaped specimens (6 mm diameter, 2 mm thickness) of nanofilled composite resin were prepared and divided into six groups. The samples were soaked in Kawa Daun solution, Robusta coffee solution, and artificial saliva for 6 and 18 hours at 37°C. Color measurements were taken before and after soaking using a Colorflex EZ spectrophotometer, and ΔE values were calculated. Data were analyzed using repeated-measures analysis of variance and post-hoc tests (<i>p</i> < 0.05).</p><p><strong>Results: </strong>Significant color changes were observed among the groups (<i>p</i> < 0.001). Soaking in Kawa Daun and Robusta coffee solutions produced significantly greater discoloration than artificial saliva, with increased ΔE values corresponding to longer soaking times.</p><p><strong>Conclusion: </strong>Prolonged exposure to Kawa Daun beverage caused measurable discoloration of nanofilled composite resin, likely due to pigment and acid content that degrade the resin matrix. These findings highlight the importance of patient education regarding the esthetic effects of traditional acidic and pigmented beverages on dental restorations.</p>","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"13 ","pages":"45575"},"PeriodicalIF":0.0,"publicationDate":"2026-04-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13135278/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147824160","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of maturation time and adhesive system on the shear bond strength and failure modes of Biodentine™ bonded with resin composite and glass ionomer cement.","authors":"Wichida Chaweewannakorn, Kittichot Saetae, Achirawat Wattakul, Suprawee Ngamjantratip, Pathida Pornraksamanee, Nirada Dhanesuan, Narinee Chinajitphan, Kwanchanok Youcharoen","doi":"10.2340/biid.v13.45898","DOIUrl":"10.2340/biid.v13.45898","url":null,"abstract":"<p><strong>Objectives: </strong>This study aimed (1) to compare the shear bond strength (SBS) and failure modes of Biodentine™ (BD) specimens bonded with resin composite (RC) or high-viscosity glass ionomer cement (HVGIC) at different maturation times and (2) to compare the SBS of BD bonded with RC using different adhesive systems.</p><p><strong>Materials and methods: </strong>Part I: A total of 120 BD specimens were bonded with either RC or HVGIC (<i>n</i> = 60 each), then divided into four groups (<i>n</i> = 15 each) based on BD maturation time: 12 min, 24 h, 48 h, and 1 week. Part 2: A total of 45 BD specimens matured for 24 h were bonded with RC using 3 adhesives (<i>n</i> = 15 each): (1) Single Bond Universal (SU) with self-etched technique (SU-SE), (2) Adper Single Bond 2 adhesive with etch and rinse technique (AS-ER), and (3) SU adhesive with etch-and-rinse technique (SU-ER). All specimens underwent pH cycling and thermocycling. SBS was measured using a universal testing machine, and failure modes were examined under a stereomicroscope.</p><p><strong>Results: </strong>BD bonded with RC showed significantly higher SBS than BD bonded with HVGIC at all BD maturation times (<i>p</i> < 0.05). No significant SBS differences were found within RC groups, except between the 24-h and 1-week. Cohesive failure predominated in RC specimens (70%), whereas adhesive failure prevailed in HVGIC specimens (71.7%). SU-ER had significantly higher SBS than SU-SE or AS-ER (<i>p</i> < 0.05).</p><p><strong>Conclusion: </strong>BD bonded with RC demonstrated higher SBS than BD bonded with HVGIC. The failure mode in the RC group was predominantly cohesive, whereas in the HVGIC group, it was primarily adhesive. These findings support the clinical applicability of immediate definitive RC placement over BD using a methacryloyloxydecyl dihydrogen phosphate-containing adhesive with an etch-and-rinse strategy.</p>","PeriodicalId":72378,"journal":{"name":"Biomaterial investigations in dentistry","volume":"13 ","pages":"45898"},"PeriodicalIF":0.0,"publicationDate":"2026-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13130177/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147824259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}