Archives of microbiology & immunology最新文献

{"title":"Characterization of GATA3 and Mammaglobin in breast tumors from African American Women.","authors":"Luisel J Ricks-Santi,&nbsp;Kristianna Fredenburg,&nbsp;Moein Rajaei,&nbsp;Ashwin Esnakula,&nbsp;Tammey Naab,&nbsp;J Tyson McDonald,&nbsp;Yasmine Kanaan","doi":"10.26502/ami.936500101","DOIUrl":"https://doi.org/10.26502/ami.936500101","url":null,"abstract":"<p><p>GATA3 and Mammaglobin are often used in the clinic to identify metastases of mammary origin due to their robust and diffuse expression in mammary tissue. However, the expression of these markers has not been well characterized in tumors from African American women. The goal of this study was to characterize and evaluate the expression of GATA3 and mammaglobin in breast tumors from African American women and determine their association with clinicopathological outcomes including breast cancer subtypes. Tissue microarrays (TMAs) were constructed from well preserved, morphologically representative tumors in archived formalin-fixed, paraffin-embedded (FFPE) surgical blocks from 202 patients with primary invasive ductal carcinoma. Mammaglobin and GATA3 expression was assessed using immunohistochemistry (IHC). Univariate analysis was carried out to determine the association between expression of GATA3, mammaglobin and clinicopathological characteristics. Kaplan-Meier estimates of overall survival and disease-free survival were also plotted and a log-rank test performed to compare estimates among groups. GATA3 expression showed statistically significant association with lower grade (p<0.001), ER-positivity (p<0.001), PR-positivity (p<0.001), and the luminal subtype (p<0.001). Mammaglobin expression was also significantly associated with lower grade (p=0.031), ER-positivity (p=0.007), and PR-positivity (p=0.022). There was no association with recurrence-free or overall survival. Our results confirm that GATA3 and mammaglobin demonstrate expression predominantly in luminal breast cancers from African American women. Additional markers with improved specificity and sensitivity are warranted for triple negative breast tumors given the high prevalence in women of African descent.</p>","PeriodicalId":72285,"journal":{"name":"Archives of microbiology & immunology","volume":"7 1","pages":"18-28"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10305425/pdf/nihms-1896850.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9754049","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Automated Image Analysis Pipeline Development to Monitor Disease Progression in Muscular Dystrophy Using Cell Profiler.","authors":"Alexandra Brown,&nbsp;Brooklyn Morris,&nbsp;John Karanja Kamau,&nbsp;Abdullah A Alshudukhi,&nbsp;Abdulrahman Jama,&nbsp;Hongmei Ren","doi":"10.26502/ami.936500115","DOIUrl":"10.26502/ami.936500115","url":null,"abstract":"<p><p>Muscular dystrophies are inherited disorders that are characterized by progressive muscle degeneration. These disorders are caused by mutations in the genes encoding structural elements within the muscle, which leads to increased vulnerability to mechanical stress and sarcolemma damage. Although myofibers have the capacity to regenerate, the newly formed myofibers still harbor genetic mutation, which induces continuous cycles of muscle fiber death and regeneration. This repeated cycling is accompanied by an inflammatory response which eventually provokes excessive fibrotic deposition. The histopathological changes in skeletal muscle tissue are central to the disease pathogenesis. Analysis of muscle histopathology is the gold standard for monitoring muscle health and disease progression. However, manual, or semi-manual quantification methods, are not only immensely tedious but can be subjective. Here, we present four image analysis pipelines built in CellProfiler which enable users without a background in computer vision or programming to quantitatively analyze biological images. These image analysis pipelines are designed to quantify skeletal muscle histopathological staining for membrane damage, the abundance and size distribution of regenerating muscle fibers, inflammation via quantification of CD68+ M1 macrophages, and collagen deposition. Additionally, we discuss methods to address common errors associated with the quantification of microscopy images. These automated tools can not only improve workflow efficiency but can provide a better understanding of the histopathological progression of muscular dystrophy.</p>","PeriodicalId":72285,"journal":{"name":"Archives of microbiology & immunology","volume":"7 3","pages":"178-187"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10552673/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41180549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Pragmatic, Individually Randomised, Open-Label, Retrospective Study of The Efficacy of Topical Therapy with Framycetin in The Treatment of Exacerbation of Chronic Nasopharyngitis","authors":"Vasyl I. Popovych, Ivana V. Koshel","doi":"10.26502/ami.936500118","DOIUrl":"https://doi.org/10.26502/ami.936500118","url":null,"abstract":"The treatment efficacy of chronic nasopharyngitis with systemic antibacterial agents is low. The ineffectiveness of antibiotic therapy is thought to be related to nasopharyngeal bacterial biofilms. The prospect is to influence the bacteria stored in the biofilm, particularly using topical antibacterial agents.","PeriodicalId":72285,"journal":{"name":"Archives of microbiology & immunology","volume":"22 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135909478","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"First Detection of NDM-type Carbapenemase-Producing K. pneumoniae isolated from Clinical Samples in Ouagadougou, Burkina Faso","authors":"Abdoul Karim Ouattara, Blandine Ouédraogo, Amana Mètuor Dabiré, Rahimatou Yasmine Wendkuni Tiemtoré, Serge Sougué, Jacques Simporé","doi":"10.26502/ami.936500121","DOIUrl":"https://doi.org/10.26502/ami.936500121","url":null,"abstract":"Background: Klebsiella pneumoniae is one of the most concerning Gram-negative opportunistic pathogens responsible for various infectious diseases. The rapid emergence and spread of clinically multidrug-resistant and hypervirulent strains of K. pneumoniae constitute a serious public health threat. The aim of this study was to identify the NDM-type carbapenemase gene in K. pneumoniae strains isolated from the Centre Hospitalier Universitaire Pédiatrique Charles De Gaulle (CHUP-CDG) de Ouagadougou, Burkina Faso.","PeriodicalId":72285,"journal":{"name":"Archives of microbiology & immunology","volume":"32 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135102772","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanism Underlying the Immune Responses of a Sublingual Vaccine for SARS-CoV-2 with RBD Antigen and Adjuvant, Poly(I:C) or AddaS03, in Non-human Primates","authors":"Tetsuro Yamamoto, Fusako Mitsunaga, Kunihiko Wasaki, Atsushi Kotani, Kazuki Tajima, Masanori Tanji, Shin Nakamura","doi":"10.26502/ami.936500113","DOIUrl":"https://doi.org/10.26502/ami.936500113","url":null,"abstract":"A sublingual vaccine formulated with recombinant SARS-CoV-2 spike protein receptor binding domain (RBD) antigen and Poly(I:C) adjuvant was assessed for its safety in non-human primates. This Poly(I:C)-adjuvanted sublingual vaccine was safe compared to the AddaS03-adjuvanted vaccine in blood tests and plasma CRP. The safety of the vaccine was also confirmed through quantitative reverse transcription PCR of six genes and ELISA of four cytokines associated with inflammation and related reactions. The Poly(I:C)- or AddaS03- adjuvanted sublingual vaccine produced RBD-specific IgA antibodies in nasal washings, saliva, and plasma. SARS-CoV-2 neutralizing antibodies were detected in plasma, suggesting that adjuvanted-sublingual vaccines protect against SARS-CoV-2 infection. “Yin and Yang” -like unique transcriptional regulation was observed through DNA microarray analyses of white blood cell RNAs from both vaccines, suppressing and enhancing immune responses and up- or downregulating genes associated with these immune responses. Poly(I:C) adjuvanted sublingual vaccination induced atypical up- or downregulation of genes related to immune suppression or tolerance; Treg differentiation; and T-cell exhaustion. Therefore, Poly(I:C) adjuvant is safe and favorable for sublingual vaccination and can induce a balanced “Yin/Yang” -like effect on immune responses.","PeriodicalId":72285,"journal":{"name":"Archives of microbiology & immunology","volume":"31 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"136139687","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Blockade Antibody Responses in Human Subjects Challenged with a New Snow Mountain Virus Inoculum.","authors":"Makoto Ibaraki, Lilin Lai, Christopher Huerta, Muktha S Natrajan, Matthew H Collins, Evan J Anderson, Mark J Mulligan, Nadine Rouphael, Christine L Moe, Pengbo Liu","doi":"10.26502/ami.936500129","DOIUrl":"10.26502/ami.936500129","url":null,"abstract":"<p><strong>Background: </strong>Noroviruses (NoVs) are a leading cause of non-bacterial gastroenteritis in young children and adults worldwide. Snow Mountain Virus (SMV) is the prototype of NoV GII genotype 2 (GII.2) that has been developed as a viral model for human challenge studies, an important tool for studying pathogenesis and immune response of NoV infections and for evaluating NoV vaccine candidates. Previous studies have identified blockade antibodies that block the binding of NoV virus-like particles (VLPs) to histo-blood group antigens (HBGAs) as a surrogate for neutralization in human Norwalk virus and GII.4 infections but little is known about SMV blockade antibodies.</p><p><strong>Methods: </strong>In this secondary data analysis study, blockade antibodies were characterized in pre-challenge and post-challenge serum samples from human subjects challenged with a new SMV inoculum. The correlation between blockade antibody geometric mean antibody titers (GMTs) and SMV-specific serum IgG/IgA GMTs were examined after stratifying the subjects by infection status. A linear mixed model was applied to test the association between HBGA blockade antibody concentrations and post-challenge days accounting for covariates and random effects.</p><p><strong>Results: </strong>Laboratory results from 33 SMV inoculated individuals were analyzed and 75.7% (25/33) participants became infected. Serum SMV-specific blockade antibodies, IgA, and IgG were all significantly different between infected and uninfected individuals beginning day 15 post-challenge. Within infected individuals, a significant correlation was observed between both IgG and IgA and blockade antibody concentration as early as day 6 post-challenge. Analysis of blockade antibody using the linear mixed model showed that infected individuals, when compared to uninfected individuals, had a statistically significant increase in blockade antibody concentrations across the post-challenge days. Among the post-challenge days, blockade antibody concentrations on days 15, 30, and 45 were significantly higher than those observed pre-challenge. The intraclass correlation coefficient (ICC) analysis indicated that the variability of blockade antibody titers is more observed between individuals rather than within subjects.</p><p><strong>Conclusions: </strong>These results indicate that HBGA-blockade antibody GMTs are generated after SMV challenge and the blockade antibodies were still detectable at day 45 post-challenge. These data indicate that the second-generation of SMV inoculum is highly effective.</p>","PeriodicalId":72285,"journal":{"name":"Archives of microbiology & immunology","volume":"7 4","pages":"318-325"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11067712/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140867745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunization with Formaldehyde Inactivated Whole Cell Vaccine against Multidrug Resistant Pseudomonas aeruginosa Produces Protective IgG Antibodies in Mice Model","authors":"Modina Ansary Nabonee, Asma Rahman, Nafisa Jabin Mishu, Nigha Zannat Dola, SM Shamsuzzaman","doi":"10.26502/ami.936500120","DOIUrl":"https://doi.org/10.26502/ami.936500120","url":null,"abstract":"The emergence of multidrug resistant (MDR) Pseudomonas aeruginosa (P. aeruginosa) are particularly challenging for clinician. Development of an effective vaccine may overcome the problems associated with treatment failure and antibiotic resistance. This study was designed to determine immune response against MDR P. aeruginosa using formaldehyde inactivated P. aeruginosa in mice model. In this study, twelve Swiss albino mice were used. Intramuscular inoculation was implemented three times at 14 days interval with formaldehyde inactivated MDR P. aeruginosa. The mice were given intraperitoneal challenge with live P. aeruginosa and observed for 14 days. Tail blood was collected to assess antigen binding capacity and bactericidal capacity of the serum antibody of immunized mice by ELISA and serum bactericidal antibody (SBA) assay, respectively. The survival rate was 100% among the mice of immunized group at 14 days of post challenge. In ELISA, OD values of serum immunoglobulin G of pre and post challenge immunized mice were significantly higher (p < .001). The SBA assay of the immunized mice sera revealed 100% bactericidal activity after 3h of incubation with 50% guinea pig complement. It was observed that formaldehyde inactivated MDR P. aeruginosa induced protective antibodies in mice model.","PeriodicalId":72285,"journal":{"name":"Archives of microbiology & immunology","volume":"36 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135102757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"May the SARS-COV2 OMICRON Variant Signal the End of the Pandemic – A Fibonacci Fractal analysis","authors":"Jean-claude Perez, V. Lounnas, Megawaty Tan, Xavier Azalbert, C. Perronne","doi":"10.26502/ami.93650072","DOIUrl":"https://doi.org/10.26502/ami.93650072","url":null,"abstract":"","PeriodicalId":72285,"journal":{"name":"Archives of microbiology & immunology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69344391","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Drug Resistance in Mycobacterium tuberculosis and its Impact on Modern Medicine","authors":"I. Amjad, S. Riaz","doi":"10.26502/ami.93650076","DOIUrl":"https://doi.org/10.26502/ami.93650076","url":null,"abstract":"Throughout the history, there has been a constant war between host and microorganisms. Microorganisms have noteworthy amplitude to offer resistance to antimicrobial agents and becoming an awful public health plight. The rising probability of infections is attributed to the ability of microbes conferring drug resistance. Drug resistance is a horrible capacity of a microbe to continue growing even in the presence of drug that is normally destined to limit its growth. As a result, there is a dwindling effect on the treatment efficacy to a disease. Drug resistance is awarded at the lethal concentration to a microbe and that particular concentration should be non-lethal to a human being. Mycobacterium tuberculosis is a greatest discovery of Robert Koch and it offers resistance to first-line and secondline antibiotics. Apprehending the mode of action of resistance for tuberculosis is crucial for advancement in the field of medical microbiology and for developing death-dealing antimicrobial drugs to minimize infections and mortality. Drug resistance is a major concern as it can lead to treatment failure and adjoins burden on healthcare costs. Next-generation sequencing technologies have helped to comprehend drug resistance. In this paper, we present the attributes of drug resistance of Mycobacterium tuberculosis and its impact on public health and challenges to modern medicine along with its epidemiology, mode of action, clinical factors and multi-drug resistance. Arch Microbiol Immunology 2022; 6 (1): 51-64 DOI: 10.26502/ ami.93650076 Arch Microbiol Immunology Vol. 6 No. 1 March 2022. 52","PeriodicalId":72285,"journal":{"name":"Archives of microbiology & immunology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69344444","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Activation of Human Dendritic Cells by Nanoparticles Carrying CTL Epitopes of Non-Structural Proteins of Hepatitis C Virus","authors":"Kuprianov Victor, Lyudmila Nikolaeva, A. Zykova, A. Dedova, Artemiy Vakhrameev, N. Ravin","doi":"10.26502/ami.93650075","DOIUrl":"https://doi.org/10.26502/ami.93650075","url":null,"abstract":"Activation of human dendritic cells by nanoparticles carrying CTL epitopes of non-structural proteins of hepatitis C virus. Archives of Microbiology and Immunology 6 (2022): 39-50. Abstract The aim of this study was to produce immunogenic nanoparticles carrying cytotoxic T lymphocyte (CTL) epitopes of hepatitis C virus non-structural proteins. We have obtained recombinant proteins forming virus-like particles and containing the sequences of self-assembling peptides (SAP), PADRE, and CTL epitopes. Using atomic force microscopy (AFM), the size of thus obtained nanoparticles was shown to be dependent on number of CTL epitopes proliferative activity and interferon-γ (IFN-γ) production by the stimulated T lymphocytes were evaluated by their secondary stimulation with commercially available mixture of peptides from non-structural proteins of hepatitis C virus. The greatest stimulating effect on T lymphocytes was exerted by DCs activated by nanoparticles, consisting of the recombinant mosaic protein with SAP at the N terminus and CTL epitopes of NS3, NS4a, and NS4b proteins at the C terminus. The presence of SAP in recombinant proteins’ sequences increased their immunogenicity. glycine linker at the BamH1-Sac1 to improve the interaction of the hexahistidine site with the Ni sorbent during purification. Nucleotide sequences optimized for E. coli encoded the recombinant proteins.","PeriodicalId":72285,"journal":{"name":"Archives of microbiology & immunology","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"69344437","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}