Immunization with Formaldehyde Inactivated Whole Cell Vaccine against Multidrug Resistant Pseudomonas aeruginosa Produces Protective IgG Antibodies in Mice Model

Abstract

The emergence of multidrug resistant (MDR) Pseudomonas aeruginosa (P. aeruginosa) are particularly challenging for clinician. Development of an effective vaccine may overcome the problems associated with treatment failure and antibiotic resistance. This study was designed to determine immune response against MDR P. aeruginosa using formaldehyde inactivated P. aeruginosa in mice model. In this study, twelve Swiss albino mice were used. Intramuscular inoculation was implemented three times at 14 days interval with formaldehyde inactivated MDR P. aeruginosa. The mice were given intraperitoneal challenge with live P. aeruginosa and observed for 14 days. Tail blood was collected to assess antigen binding capacity and bactericidal capacity of the serum antibody of immunized mice by ELISA and serum bactericidal antibody (SBA) assay, respectively. The survival rate was 100% among the mice of immunized group at 14 days of post challenge. In ELISA, OD values of serum immunoglobulin G of pre and post challenge immunized mice were significantly higher (p < .001). The SBA assay of the immunized mice sera revealed 100% bactericidal activity after 3h of incubation with 50% guinea pig complement. It was observed that formaldehyde inactivated MDR P. aeruginosa induced protective antibodies in mice model.