Advanced Biomedical Research最新文献_第8页

Diagnosis and Treatment of Coagulopathy Caused by the New Coronavirus: A Systematic Review and Meta-Analysis Protocol. 新型冠状病毒致凝血功能障碍的诊断和治疗:一项系统综述和荟萃分析方案。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_403_21

Sadegh Abbasian, Mahya Razmi, Hadiseh Bahramian, Mostafa Shanbehzadeh, Hadi Kazemi-Arpanahi

{"title":"Diagnosis and Treatment of Coagulopathy Caused by the New Coronavirus: A Systematic Review and Meta-Analysis Protocol.","authors":"Sadegh Abbasian, Mahya Razmi, Hadiseh Bahramian, Mostafa Shanbehzadeh, Hadi Kazemi-Arpanahi","doi":"10.4103/abr.abr_403_21","DOIUrl":"https://doi.org/10.4103/abr.abr_403_21","url":null,"abstract":"Background: The new coronavirus is an agent of respiratory infections associated with thrombosis in vital organs. This study aimed to propose a better diagnosis and treatment of coagulation disorders caused by the new coronavirus (Covid-19).Materials and methods: Search in Cochrane central, Web of Science, PubMed, Scopus, and Ovid will be done. Also, according to the inclusion criteria, cross-sectional studies, cohort, clinical trial, and case-control will be included without gender and language restriction. Participants will also be Covid-19 patients with coagulation disorders. Any disagreement in the stages of screening, selection, and extraction of data between the two reviewers will be resolved by discussion, then if not resolved, the opinion of expert reviewers will be used. The risk of bias will be assessed using the NOS (Newcastle-Ottawa scale) tool for cross-sectional study, cohort and case-control, and the Cochrane checklist for clinical trials study. Metaanalysis of included studies that are similar based on the methodology will be done. Also, a fixed or random-effect model will be used for this it. Heterogeneity indices (I2), odds ratio (OR), risk ratio (RR), mean difference, and %95 confidence interval will also be calculated by Stata V.13.0 (Corporation, College Station TX).Results: Treatment with anticoagulants will reduce the severity of thrombosis and lung disease in patients. D-dimer measurement will also be a diagnosis indicator of thrombosis.Conclusions: Simultaneous study of coagulation disorders and thrombosis in patients and development of a Godliness based on it will play a treatment role in the follow-up of the coronavirus disease.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"147"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/04/66/ABR-12-147.PMC10410409.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9976184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Non-Hodgkin Lymphoma of Cauda Equina: A Diagnostic Conundrum: Case Report. 马尾非霍奇金淋巴瘤:一个诊断难题:病例报告。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_36_21

Pranati Misra, Ranjita Panigrahi, Prita Pradhan, Debashis Pothal, Urmila Senapati, Narendra K Das

引用次数: 0

The Necessity of Using Strand-Specific cDNA for Achieving Accurate Transcriptome Analysis Result. 利用链特异性cDNA获得准确转录组分析结果的必要性。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_102_22

Reyhaneh Dehghanzad, Anis Khalafiyan, Hossein Khanahmad

{"title":"The Necessity of Using Strand-Specific cDNA for Achieving Accurate Transcriptome Analysis Result.","authors":"Reyhaneh Dehghanzad, Anis Khalafiyan, Hossein Khanahmad","doi":"10.4103/abr.abr_102_22","DOIUrl":"https://doi.org/10.4103/abr.abr_102_22","url":null,"abstract":"The importance of transcriptome analysis results due to their critical roles in genome annotation and subsequently progression in prevention, prognosis, diagnosis, and treatment of disease, led us on sharing our concern about the accuracy level of these existent data and meta‐analysis on them. Transcriptome analysis, especially RNA sequencing (RNA‐seq), has increasingly provided new insights for understanding gene structure, expression, and regulation and has been developed in some methodology.[1,2] There are regions in the genome (nearly 10% of the human genome loci) that produce both coding and noncoding isoforms that are called bifunctional RNAs and were confirmed and annotated by NCBI. The balance between noncoding and coding RNAs levels are affected by both physiologic process like development stages and differentiation and also environmental factors such as drug, physical or chemical agent or viral, bacterial or fungal pathogens.[3] Moreover, information in antisense transcripts plays important role in transcriptome profiling according to their critical roles in biological functions, as well as, by affecting accurately quantifying sense gene expression, particularly for genes with the overlapping loci and opposite direction of transcription. Therefore, discrimination between coding and noncoding isoforms is crucial to prevent misinterpretation of RNA‐Seq data.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"108"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a4/34/ABR-12-108.PMC10241614.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9591421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The Effect of Stem Cells and Vascular Endothelial Growth Factor on Cancer Angiogenesis. 干细胞和血管内皮生长因子对肿瘤血管生成的影响。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_378_21

Nahid Moradi-Gharibvand, Batool Hashemibeni

{"title":"The Effect of Stem Cells and Vascular Endothelial Growth Factor on Cancer Angiogenesis.","authors":"Nahid Moradi-Gharibvand, Batool Hashemibeni","doi":"10.4103/abr.abr_378_21","DOIUrl":"https://doi.org/10.4103/abr.abr_378_21","url":null,"abstract":"The formation of new vessels from pre-existing vessels is known as angiogenesis. The process is controlled by stimuli and inhibitors. Angiogenesis starts as a result of the unbalance of these factors, where balance has a tendency toward the stimulus. One of the most important factors promoting angiogenesis is the vascular endothelial growth factor (VEGF). In addition to being involved in vascular regeneration in normal tissues, VEGF also takes part in tumor tissue angiogenesis. These factors affect endothelial cells (ECs) directly as well as differentiate tumor cells from endothelial cells and play an active role in tumor tissue angiogenesis. Angiogenesis partakes in the growth and proliferation of tumor tissue. Because anti-angiogenic treatment is favorable in existing cancer therapies, the potential benefits should be considered. One of these new therapies is cell therapy using mesenchymal stem cells (MSCs). Research on MSCs remains controversial because much of the earlier research on MSCs has shown their effectiveness, but more recent research has identified harmful effects of these cells. This article reviews the role of stem cells and their secretions in the angiogenesis of tumor tissues.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"124"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/63/5f/ABR-12-124.PMC10331557.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9869904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Comparison of the Effect of Preoperative Oral Tizanidine and Pregabalin on Shoulder Pain in Laparoscopic Cholecystectomy Under General Anesthesia. 术前口服替扎尼定与普瑞巴林治疗全麻腹腔镜胆囊切除术肩关节疼痛的疗效比较。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_222_22

Shahryar Sane, Shahram Sayyadi, Rahman Abbasivash, Seyfollah Rezaei, Ashkan Azadfar, Sohrab Salimi

{"title":"Comparison of the Effect of Preoperative Oral Tizanidine and Pregabalin on Shoulder Pain in Laparoscopic Cholecystectomy Under General Anesthesia.","authors":"Shahryar Sane, Shahram Sayyadi, Rahman Abbasivash, Seyfollah Rezaei, Ashkan Azadfar, Sohrab Salimi","doi":"10.4103/abr.abr_222_22","DOIUrl":"https://doi.org/10.4103/abr.abr_222_22","url":null,"abstract":"Background: Shoulder pain is considered as the most important and relatively common postoperative cholecystectomy complications that often controls in recovery room by systemic narcotics that may have some side effects. The aim of this study was to evaluate the effect of premedication with oral tizanidine on shoulder pain relief after elective laparoscopic cholecystectomy.Materials and methods: In this double-blinded clinical trial, 75 adults of American Society of Anesthesiologist physiologic state 1 and 2 scheduled for elective laparoscopic cholecystectomy under general anesthesia were selected and randomly divided in three groups of T, P, and control groups. Ninety minutes before the induction of anesthesia, patients received either 4 mg tizanidine (T group), 100 mg pregabalin (P group), or orally in 50cc or the same volume of plain water as a placebo (control group). Then, the vital signs, pain intensity, and the need for analgesic were measured during 24 hours and then compared in the groups.Results: There was no significant difference in patient characteristics, with respect to age, weight, gender, and duration of anesthesia and surgery between the groups (P > 0.05). The pain intensity and need for analgesic were significantly lower in tizanidine and pregabalin groups than the control group (P < 0.003) vs (P < 0.001). There was no significant difference in vital signs characteristics between the groups.Conclusion: Oral administration of 4 mg tizanidine and 100 mg pregabalin 90 minutes before laparoscopic cholecystectomy significantly relive postoperative shoulder pain and analgesic consumption without any complication.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"58"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/10/43/ABR-12-58.PMC10186033.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9483848","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Perioperative Anxiolysis and Analgesic Effect after Premedication with Melatonin and Pregabalin in Total Hip Arthroplasty under Spinal Anaesthesia: A Prospective Comparative Trial. 脊髓麻醉下全髋关节置换术术前应用褪黑素和普瑞巴林后围手术期的焦虑缓解和镇痛效果:一项前瞻性比较试验。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_323_22

Akash Mishra, Vinod Kumar Srivastava, Ravi Prakash, Neel Kamal Mishra, Jyotsna Agarwal, Shruti Kabi

{"title":"Perioperative Anxiolysis and Analgesic Effect after Premedication with Melatonin and Pregabalin in Total Hip Arthroplasty under Spinal Anaesthesia: A Prospective Comparative Trial.","authors":"Akash Mishra, Vinod Kumar Srivastava, Ravi Prakash, Neel Kamal Mishra, Jyotsna Agarwal, Shruti Kabi","doi":"10.4103/abr.abr_323_22","DOIUrl":"https://doi.org/10.4103/abr.abr_323_22","url":null,"abstract":"Background: Preoperative anxiety plays a critical role in post-operative pain response and other outcomes. Melatonin is a naturally secreted hormone which has anxiolytic, sedative, and analgesic properties. Pregabalin, analogue of gabapentin which has property of anxiolytic and analgesic effects.Materials and methods: Total 96 patients undergoing total hip arthroplasty, divided into 3 groups of 32 each and were given placebo (group I), melatonin 6 mg (group II), and pregabalin 150 mg (group III). Anxiety level, postoperative pain score, sedation level and duration as well as characteristics of spinal anaesthesia were assessed with other vital parameters.Results: Group I showed an increment in the anxiety score from baseline whereas in group II and group III, there was a decline in pre-operative anxiety score from baseline at all the periods of observation and more significantly in group III. Visual analogue scale (VAS) score and total dose of rescue analgesia were highest in group I, but group II and group III were comparable to each other. However, the durations of spinal anaesthesia and motor blockade showed a statistically significant difference with maximum duration in group III followed by II and then I. The level of sedation among the three groups were comparable at all the periods of observation.Conclusions: Pregabalin was found better for perioperative anxiolysis, post-operative analgesia and for prolongation of duration of spinal anaesthesia when compared to melatonin.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"185"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/2e/c4/ABR-12-185.PMC10492595.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10223598","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Human Respiratory Syncytial Virus and Coronavirus Rates in Iranian Elderly Patients More than 60 Years Old with Acute Respiratory Symptoms. 伊朗60岁以上急性呼吸道症状老年人呼吸道合胞体病毒和冠状病毒感染率

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_395_22

Shaian Tavakolian, Hossein Goudarzi, Ilad Alavi Darazam, Ebrahim Faghihloo

{"title":"Human Respiratory Syncytial Virus and Coronavirus Rates in Iranian Elderly Patients More than 60 Years Old with Acute Respiratory Symptoms.","authors":"Shaian Tavakolian, Hossein Goudarzi, Ilad Alavi Darazam, Ebrahim Faghihloo","doi":"10.4103/abr.abr_395_22","DOIUrl":"https://doi.org/10.4103/abr.abr_395_22","url":null,"abstract":"Background: Scientists have believed that a number of risk factors, especially viral infectious agents, can be related to respiratory diseases. Due to the pandemics in 2019, Human Respiratory Syncytial Virus and Coronavirus have attracted the attention of different kinds of research. In this study, we attempted to evaluate the prevalence of these viruses.Materials and methods: After extracting the RNA and DNA of these viruses, molecular tests were employed to report the rate of them in patients suffering from respiratory symptoms.Results: Our results demonstrated that 31 samples were COVID-19 positive. Furthermore, two cases had Respiratory syncytial virus (RSV) subgroup A infections. However, no cases showed a coinfection of both viruses.Conclusions: It seems that during the pandemic of COVID-19, RSV should not be ignored as it can be responsible for the respiratory syndrome.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"180"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d2/fc/ABR-12-180.PMC10492601.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10222223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Dichloromethane and N-Butanol Fractions of Nigella sativa on ACHN and GP-293 Cell Line Morphology, Viability, and Apoptosis. 二氯甲烷和正丁醇组分对黑草ACHN和GP-293细胞株形态、活力和凋亡的影响

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_394_22

Samira Shahraki, Sara Hosseinian, Elham Shahraki, Mehdi Kheirandish, Abolfazl Khajavirad

{"title":"Effects of Dichloromethane and N-Butanol Fractions of Nigella sativa on ACHN and GP-293 Cell Line Morphology, Viability, and Apoptosis.","authors":"Samira Shahraki, Sara Hosseinian, Elham Shahraki, Mehdi Kheirandish, Abolfazl Khajavirad","doi":"10.4103/abr.abr_394_22","DOIUrl":"https://doi.org/10.4103/abr.abr_394_22","url":null,"abstract":"Background: Renal cell carcinoma (RCC) is among the top death-causing cancers. Medicinal herbs can also have beneficial effects on RCC treatment. In this project, we aimed to study the antitumor effect of dichloromethane and N-butanol fractions of hydroalcoholic extract of Nigella sativa (N. sativa) on the morphology, viability, and apoptosis of ACHN (human renal adenocarcinoma) and GP-293 (normal renal epithelial) cell lines.Materials and methods: In this experimental study, N-butanol and dichloromethane fractions of N. sativa were obtained, and ACHN and GP293 cell lines were treated with various concentrations of dichloromethane (0-100 μg/mL) and N-butanol (0-12.5 μg/mL) fractions for 24, 48, and 72 hours. Then, morphological changes, viability, and apoptosis were investigated.Results: Our results indicated that dichloromethane and N-butanol fractions cause morphological changes and significant decreases in the percentage of live cells in the ACHN cell line, in a dose- and time-dependent manner. In the GP-293 cell line, however, a lower toxicity was observed in comparison with that found for ACHN. The results of flow cytometry showed an apoptotic effect of dichloromethane and N-butanol fractions on the ACHN cell line but a higher rate of apoptosis induction for the total extract compared to the two fractions in the renal cancer cell line compared to the normal cell line.Conclusion: Our findings demonstrated that these two fractions of N. sativa induce inhibitory effects on the ACHN cell line morphology and viability. These effects were lower than those induced by the total extract. In addition, the two fractions caused more marked effects in the renal cancer cell line compared with the GP-293 cell line.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"200"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fe/71/ABR-12-200.PMC10492620.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10220083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Status of Hypertension Control in Urban Slums of Central India: A community Health Worker-Based Two-Year Follow-Up. 印度中部城市贫民窟高血压控制状况:以社区卫生工作者为基础的两年随访。

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_266_22

Abhijit Pakhare, Anuja Lahiri, Neelesh Shrivastava, Krishna N Subba, Vaishnavi Veera Durga Kurra, Ankur Joshi, Shubham Atal, Sagar Khadanga, Rajnish Joshi

{"title":"Status of Hypertension Control in Urban Slums of Central India: A community Health Worker-Based Two-Year Follow-Up.","authors":"Abhijit Pakhare, Anuja Lahiri, Neelesh Shrivastava, Krishna N Subba, Vaishnavi Veera Durga Kurra, Ankur Joshi, Shubham Atal, Sagar Khadanga, Rajnish Joshi","doi":"10.4103/abr.abr_266_22","DOIUrl":"https://doi.org/10.4103/abr.abr_266_22","url":null,"abstract":"Background: Hypertension (HTN) is a leading cause of cardiovascular diseases and its control is poor. There is heterogeneity in levels of blood pressure control among various population subgroups. The present study was conducted within the framework of the National Program for Prevention and Control of Cancer, Diabetes, Cardiovascular Diseases, and Stroke (NPCDCS) in India. It aims to estimate the proportion of optimal blood pressure control and identify factors associated with uncontrolled HTN consequent to initial screening.Materials and methods: We assembled a cohort of individuals with HTN confirmed in a baseline screening in sixteen urban slum clusters of Bhopal (2017-2018). Sixteen accredited social health activists were trained from within these slums. Individuals with HTN were linked to primary care providers and followed up for the next two years. Obtaining optimal blood pressure control (defined as SBP <140 and DBP <90 mm of Hg) was a key outcome.Results: Of a total of 6174 individuals, 1571 (25.4%) had HTN, of which 813 were previously known and 758 were newly detected during the baseline survey. Two-year follow-up was completed for 1177 (74.9%). Blood pressure was optimally controlled in 301 (26%) at baseline and in 442 (38%) individuals at two years (an absolute increase of 12%; 95% CI 10.2-13.9). Older age, physical inactivity, higher body mass index, and newly diagnosed HTN were significantly associated with uncontrolled blood pressure.Conclusion: We found about six of every ten individuals with HTN were on treatment, and about four were optimally controlled. These findings provide a benchmark for NPCDCS, in terms of achievable goals within short periods of follow-up.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"197"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fb/52/ABR-12-197.PMC10492608.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10223596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

microRNAs regulate survivin in colorectal cancer patients microrna调节结直肠癌患者的生存素

Advanced Biomedical Research Pub Date : 2023-01-01 DOI: 10.4103/abr.abr_233_22

Mortaza Raeisi, Hadi Chavoshi, Soghra Bornehdeli, Milad Asadi, Roya Dolatkhah, Ayse Caner

{"title":"microRNAs regulate survivin in colorectal cancer patients","authors":"Mortaza Raeisi, Hadi Chavoshi, Soghra Bornehdeli, Milad Asadi, Roya Dolatkhah, Ayse Caner","doi":"10.4103/abr.abr_233_22","DOIUrl":"https://doi.org/10.4103/abr.abr_233_22","url":null,"abstract":"Background: Impaired levels of surviving are associate with increased survival of tumor cells. In this study, we intended to profile the microRNAs (miRNAs) targeting survivin in the tumoral and marginal tissues obtained from Iranian patients with colorectal cancer (CRC).Materials and Methods: Fifty CRC patients of Iranian Azari ethnicity were recruited. The RNA content of the tumoral and marginal tissues was isolated and the transcript levels of miR-34a, miR-16, miR-150, and miR-203a and survivin were determined through quantitative Real-time PCR.Results: The mRNA expression of survivin was significantly increased (fold change = 3.21, P = 0.0029) in the tumoral tissues in comparison to the marginal tissues. There was significant downregulation of miR-16 (fold change = 0.28, P = 0.003) and miR-203a (fold change = 0.36, P = 0.014) in the tumoral samples in comparison to marginal samples. There was an inverse significant correlation (rho = -0.81; P < 0.001) between the expression of miR-203a and mRNA expression of survivin in the tumoral tissues of CRC patients. The mRNA expression of survivin was higher significantly in the tumoral tissues of CRC patients with lymph node metastasis in comparison to those without lymph node metastasis (P = 0.020). In addition, there was a significantly higher miR-203 expression level in the tumoral tissues of CRC patients with lymph node metastasis in comparison to those without lymph node metastasis (P = 0.011).Conclusion: It is suggested that miR-203 plays an oncogenic role in CRC cancer by regulating survivin and lymph node metastasis.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"2015 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135799181","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0