Advanced Biomedical Research最新文献

{"title":"Evaluation of the Relationship between Pregnancy-Associated Plasma Protein A (PAPP-A) and Pregnancy Outcomes.","authors":"Minoo Movahedi,&nbsp;Somayeh Khanjani,&nbsp;Zahra Shahshahan,&nbsp;Maryam Hajihashemi,&nbsp;Farinaz Farahbod,&nbsp;Elaheh Shahsavandi","doi":"10.4103/abr.abr_344_21","DOIUrl":"https://doi.org/10.4103/abr.abr_344_21","url":null,"abstract":"<p><strong>Background: </strong>In the current study, we aimed to evaluate the association between pregnancy-related plasma protein-A (PAPP-A) levels measured in the first trimester and pregnancy outcomes.</p><p><strong>Materials and methods: </strong>This is a descriptive-analytical study that was performed in 2019--2021 on 1061 pregnant women in their first trimester. Demographic and basic information of all women were collected. These data included age, weight, parity, and date of delivery. Then the quantity of PAPP-A was recorded in three groups including less than 0.5 MOM, 0.5 to 2.5 MOM, and more than 2.5 MOM.</p><p><strong>Results: </strong>Data of 1061 women were analyzed. 900 women (84.8%) had term delivery and 155 women (14.6%) had pre-term deliveries. PAPP-A levels were normal in 83.4% of women. BMI and number of pregnancies had significant relationships with PAPP-A (<i>p</i> < 0.001, <i>P</i> = 0.03 respectively). The mean BMI in mothers with PAPP-A higher than 2.5 was significantly more than mothers with normal or lower PAPP-A levels (26.2 ± 31, <i>P</i> = 0.04). The frequency of term labor in mothers with normal PAPP-A was higher than other mothers (86.3%, <i>P</i> = 0.04). The frequency of preeclampsia in recent pregnancies in mothers with normal PAPP-A was significantly lower than other mothers (<i>p</i> < 0.001) and the frequency of abortions in recent pregnancies in mothers with PAPP-A less than 0.5 was significantly higher than mothers with normal or elevated PAPP-A (<i>p</i> < 0.001).</p><p><strong>Conclusion: </strong>Mothers with low PAPP-A levels are more likely to have poor pregnancy outcomes such as abortion, pre-term labor, and preeclampsia.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"91"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/e3/88/ABR-12-91.PMC10241634.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9597314","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of Breast Scintigraphy for Patients with Suspicious (Breast Imaging-Reporting and Data System IV) Breast Lesions.","authors":"Ali Reza Azarpeikan,&nbsp;Ramesh Omranipour,&nbsp;Habibollah Mahmoodzadeh,&nbsp;Seyed Rouhollah Miri,&nbsp;Narjes Mohammadzadeh,&nbsp;Farhang Derakhshan,&nbsp;Saeed Farzanefar,&nbsp;Mehrshad Abbasi","doi":"10.4103/abr.abr_347_21","DOIUrl":"https://doi.org/10.4103/abr.abr_347_21","url":null,"abstract":"<p><strong>Background: </strong>The surgery for a breast imaging-reporting and data system (BIRADS) IV lesions needs imaging or pathology supporting data. The roll of breast scintigraphy for this purpose is unclear.</p><p><strong>Materials and methods: </strong>In a prospective design, 16 patients with 25 BIRADS IV lesions who were scheduled for surgery were included. Before the surgery, breast scintigraphy was done using a nondedicated dual head gamma camera in the prone position employing a shaped foam pad providing imaging at breast pendulous position. Twenty mCi^99m Tc methoxy-isobutyl-isonitrile was injected and two 15 and 60-min delayed imaging were done (anterior, bilateral, and single photon emission computed tomography [SPECT] projections). Pathology reports were collected and tumor to nontumor uptake ratio (T/NT) was analyzed, accordingly.</p><p><strong>Results: </strong>Out of all lesions, 12 were malignant (invasive ductal and lobular carcinoma ductal carcinoma <i>in situ</i>). At 15 min, T/NT was insignificantly higher in the malignant compared to benign lesions (22.8 ± 23.9 vs. 10.1 ± 10.1; <i>P</i> = 0.109). The optimal T/NT cutoff for discrimination of malignant and benign lesions was 20. Only 1 out of 13 benign lesions presented uptake >20 (7.7%; false-positive rate; <i>P</i> = 0.047). The diagnostic accuracy, sensitivity, and specificity for T/NT calculated at 0.68, 0.42, and 0.92, respectively. The T/NT at 60 min remained unchanged for either benign or malignant lesions (22.3 ± 30.2 vs. 11.7 ± 17.1; <i>P</i> = 0.296).</p><p><strong>Conclusions: </strong>Breast scintigraphy with general purpose gamma camera employing SPECT imaging may assist the selection of BIRADS IV lesions in need for surgery. All uptake positive cases should undergo surgery and decision for uptake negative cases should be made based on other data.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"86"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/57/d5/ABR-12-86.PMC10241632.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9597315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of Fetal and Maternal Outcomes in Chorion Villus Sampling (CVS).","authors":"Minoo Movahedi,&nbsp;Farinaz Farahbod,&nbsp;Elahe Zarean,&nbsp;Maryam Hajihashemi,&nbsp;Fedyeh Haghollahi,&nbsp;Mehri Farahmand","doi":"10.4103/abr.abr_229_22","DOIUrl":"https://doi.org/10.4103/abr.abr_229_22","url":null,"abstract":"<p><strong>Background: </strong>Chorionic villus sampling (CVS) is one of the invasive diagnostic methods used to diagnose chromosomal, genetic, and metabolic diseases in the embryonic period. The use of this method is associated with maternal and fetal consequences, the most serious of which is abortion. Therefore, the present study was conducted to investigate the incidence of these consequences and the factors affecting the incidence of abortion.</p><p><strong>Materials and methods: </strong>A cross-sectional study was performed on 98 pregnant women with CVS indications. Maternal and fetal outcomes including abortion, vaginal bleeding, subchorionic hematoma, premature rupture of membrane (PROM), chorioamnionitis, preterm delivery, limb abnormality, fetal growth retardation, and preeclampsia were recorded.</p><p><strong>Results: </strong>The results of the present study showed that the incidence of fetal outcomes including fetal growth failure, premature rupture of membranes, abortion, and limb abnormalities was 4.1%, 7.1%, 3.1%, and 1%, and the incidence of maternal outcomes including preterm delivery, subchorionic hematoma, preeclampsia, and hemorrhage was 14.3%, 3.1%, 6.1%, and 10.2%, respectively. In addition, a decrease in free BHCG and an increase in NT were significantly associated with the occurrence of abortion (OR: 0.11 and 4.25, respectively, <i>P</i> value < 0.05).</p><p><strong>Conclusion: </strong>It should be noted that due to a long time between placental sampling and the occurrence of vaginal bleeding, premature rupture of membrane, and preterm delivery, it seems that placental sampling has no effect. In addition, only a decrease in free BHCG or an increase in NT significantly increased the chance of miscarriage.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"133"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/6a/3a/ABR-12-133.PMC10331521.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9814970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Lieberman Community Return Program on Reducing Positive and Negative Symptoms and Improving Social Skills in Patients with Schizophrenia.","authors":"Maryam Emami,&nbsp;Gholamreza Kheirabadi,&nbsp;Mona Fallahi","doi":"10.4103/abr.abr_21_21","DOIUrl":"https://doi.org/10.4103/abr.abr_21_21","url":null,"abstract":"<p><strong>Background: </strong>The aim of this study was to investigate the effect of Lieberman community return program on reducing positive and negative symptoms and improving social skills in people with schizophrenia.</p><p><strong>Materials and methods: </strong>In this clinical trial study, 58 patients with schizophrenia were randomly allocated into two groups of 29. The first group received 16 sessions of Lieberman community return training and the second group received routine care as a control group. All patients were evaluated before intervention and 1 and 3 months after intervention using the Matson Social Skills Questionnaire and Negative and Positive Symptoms Assessment Scale and compared between the two groups.</p><p><strong>Results: </strong>Evaluation of negative symptoms showed that the dimensions of affective flattening, avolition, anhedonia-asociality, attention, and alogia in the intervention group decreased significantly over time (<i>P</i> < 0.05), but no significant difference was seen in the control group. The mean score of positive symptoms such as hallucinations, delusion, inappropriate affect, and formal thinking disorder in the intervention group were decreased significantly (<i>P</i> < 0.05), but no significant difference was seen in the control group. Appropriate social skills and overall skill score were increased significantly in the intervention group over time (<i>P</i> < 0.05).</p><p><strong>Conclusion: </strong>Lieberman community return program is likely to reduce the symptoms of schizophrenia and increase patients' social skills.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"146"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/d2/b4/ABR-12-146.PMC10410411.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9969657","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis and Treatment of Coagulopathy Caused by the New Coronavirus: A Systematic Review and Meta-Analysis Protocol.","authors":"Sadegh Abbasian,&nbsp;Mahya Razmi,&nbsp;Hadiseh Bahramian,&nbsp;Mostafa Shanbehzadeh,&nbsp;Hadi Kazemi-Arpanahi","doi":"10.4103/abr.abr_403_21","DOIUrl":"https://doi.org/10.4103/abr.abr_403_21","url":null,"abstract":"<p><strong>Background: </strong>The new coronavirus is an agent of respiratory infections associated with thrombosis in vital organs. This study aimed to propose a better diagnosis and treatment of coagulation disorders caused by the new coronavirus (Covid-19).</p><p><strong>Materials and methods: </strong>Search in Cochrane central, Web of Science, PubMed, Scopus, and Ovid will be done. Also, according to the inclusion criteria, cross-sectional studies, cohort, clinical trial, and case-control will be included without gender and language restriction. Participants will also be Covid-19 patients with coagulation disorders. Any disagreement in the stages of screening, selection, and extraction of data between the two reviewers will be resolved by discussion, then if not resolved, the opinion of expert reviewers will be used. The risk of bias will be assessed using the NOS (Newcastle-Ottawa scale) tool for cross-sectional study, cohort and case-control, and the Cochrane checklist for clinical trials study. Metaanalysis of included studies that are similar based on the methodology will be done. Also, a fixed or random-effect model will be used for this it. Heterogeneity indices (I2), odds ratio (OR), risk ratio (RR), mean difference, and %95 confidence interval will also be calculated by Stata V.13.0 (Corporation, College Station TX).</p><p><strong>Results: </strong>Treatment with anticoagulants will reduce the severity of thrombosis and lung disease in patients. D-dimer measurement will also be a diagnosis indicator of thrombosis.</p><p><strong>Conclusions: </strong>Simultaneous study of coagulation disorders and thrombosis in patients and development of a Godliness based on it will play a treatment role in the follow-up of the coronavirus disease.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"147"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/04/66/ABR-12-147.PMC10410409.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9976184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Stem Cells and Vascular Endothelial Growth Factor on Cancer Angiogenesis.","authors":"Nahid Moradi-Gharibvand,&nbsp;Batool Hashemibeni","doi":"10.4103/abr.abr_378_21","DOIUrl":"https://doi.org/10.4103/abr.abr_378_21","url":null,"abstract":"<p><p>The formation of new vessels from pre-existing vessels is known as angiogenesis. The process is controlled by stimuli and inhibitors. Angiogenesis starts as a result of the unbalance of these factors, where balance has a tendency toward the stimulus. One of the most important factors promoting angiogenesis is the vascular endothelial growth factor (VEGF). In addition to being involved in vascular regeneration in normal tissues, VEGF also takes part in tumor tissue angiogenesis. These factors affect endothelial cells (ECs) directly as well as differentiate tumor cells from endothelial cells and play an active role in tumor tissue angiogenesis. Angiogenesis partakes in the growth and proliferation of tumor tissue. Because anti-angiogenic treatment is favorable in existing cancer therapies, the potential benefits should be considered. One of these new therapies is cell therapy using mesenchymal stem cells (MSCs). Research on MSCs remains controversial because much of the earlier research on MSCs has shown their effectiveness, but more recent research has identified harmful effects of these cells. This article reviews the role of stem cells and their secretions in the angiogenesis of tumor tissues.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"124"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/63/5f/ABR-12-124.PMC10331557.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9869904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the Effectiveness of Two Types of Commercial Endotracheal Tube Holders, with the Conventional Method in a Manikin Model.","authors":"Mehdi Nasr Isfahani,&nbsp;Alireza Abootalebi,&nbsp;Khatere Ghaznavi,&nbsp;Leila Kamali Dolatabadi","doi":"10.4103/abr.abr_192_21","DOIUrl":"https://doi.org/10.4103/abr.abr_192_21","url":null,"abstract":"<p><strong>Background: </strong>Endotracheal intubation is employed to create a safe airway in patients requiring mechanical ventilation. The relatively high prevalence rate of unplanned displacement of the endotracheal tube (ETT) can be associated with serious complications. This study was conducted to evaluate the effectiveness of a newly designed tube holder in Iran (Irafit), the Thomas ETT holder and the traditional method using adhesive tape.</p><p><strong>Materials and methods: </strong>The present manikin-based study was performed on the human mannequin. For this purpose, the mannequin was first subjected to oral intubation by a skilled emergency medicine specialist. Then, three methods of adhesive tape, Irafit-ETT holder, and Thomas-ETT holder were used. The mean of displacement in width of the mouth, length of the ETT, and depth as well as ETT removal was recorded.</p><p><strong>Results: </strong>The results of the present study revealed that the displacement in depth was significantly less in the Irafit-ETT holder as compared with the other two groups following the application of a tug (<i>P</i> < 0.001). The displacement in the length of the ETT with and without the application of a tug was significantly less in the Irafit-ETT holder and Thomas-ETT holder groups as compared with the adhesive tape group (<i>P</i> < 0.001).</p><p><strong>Conclusion: </strong>According to the results of the present study, it can be stated that both ETT holder devices (Thomas vs. Irafit) were not distinct in terms of displacements in length and width; however, the Iranian model was more successful in minimizing the displacement in depth.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"30"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/85/6d/ABR-12-30.PMC10086637.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9298835","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Success Rate and Safety of Nasotracheal Intubation by Conventional and Finger-Guided Method in Patients Undergoing Maxillofacial Surgery.","authors":"Seyed Jalal Hashemi,&nbsp;Hamidreza Shetabi,&nbsp;Reihanak Talakoub,&nbsp;Avishan Aminizad","doi":"10.4103/abr.abr_255_21","DOIUrl":"https://doi.org/10.4103/abr.abr_255_21","url":null,"abstract":"<p><strong>Background: </strong>Different techniques have been introduced to reduce the complications of nasotracheal intubation. The aim of this study was to compare the incidence of nasotracheal intubation complications in finger-guided and conventional methods.</p><p><strong>Materials and methods: </strong>In this double-blind randomized trial study, 70 patients who were candidates for oral and maxillofacial surgery who required nasal intubation were included in the study finally of which 33 patients with conventional method and 35 patients with finger-guided tubes in the nasopharynx were analyzed at the end of the study. Variables such as success rate, hemodynamic response, and complications of intubation were compared between the two groups.</p><p><strong>Results: </strong>There was no significant difference between the two groups in terms of hemodynamic response to intubation (<i>P</i> > 0.05). There was a significant difference between the two groups in terms of success in tracheal intubation (<i>P</i> < 0.05). There was a significant difference between the two groups in terms of epistaxis immediately after intubation (<i>P</i> < 0.05). There was no significant difference between the two groups in terms of nasal turbine fractures (<i>P</i> > 0.05). However, the frequency of submucosal intubation in the conventional method was significantly higher than the other group (<i>P</i> = 0.02).</p><p><strong>Conclusion: </strong>Nasotracheal intubation using the finger guiding technique in the nasopharynx is associated with a higher success rate and less complications after intubation such as epistaxis and submucosal intubation compared to the conventional method.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"35"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/93/dd/ABR-12-35.PMC10086639.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9305338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Necessity of Using Strand-Specific cDNA for Achieving Accurate Transcriptome Analysis Result.","authors":"Reyhaneh Dehghanzad,&nbsp;Anis Khalafiyan,&nbsp;Hossein Khanahmad","doi":"10.4103/abr.abr_102_22","DOIUrl":"https://doi.org/10.4103/abr.abr_102_22","url":null,"abstract":"The importance of transcriptome analysis results due to their critical roles in genome annotation and subsequently progression in prevention, prognosis, diagnosis, and treatment of disease, led us on sharing our concern about the accuracy level of these existent data and meta‐analysis on them. Transcriptome analysis, especially RNA sequencing (RNA‐seq), has increasingly provided new insights for understanding gene structure, expression, and regulation and has been developed in some methodology.[1,2] There are regions in the genome (nearly 10% of the human genome loci) that produce both coding and noncoding isoforms that are called bifunctional RNAs and were confirmed and annotated by NCBI. The balance between noncoding and coding RNAs levels are affected by both physiologic process like development stages and differentiation and also environmental factors such as drug, physical or chemical agent or viral, bacterial or fungal pathogens.[3] Moreover, information in antisense transcripts plays important role in transcriptome profiling according to their critical roles in biological functions, as well as, by affecting accurately quantifying sense gene expression, particularly for genes with the overlapping loci and opposite direction of transcription. Therefore, discrimination between coding and noncoding isoforms is crucial to prevent misinterpretation of RNA‐Seq data.","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"108"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/a4/34/ABR-12-108.PMC10241614.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9591421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Dichloromethane and N-Butanol Fractions of <i>Nigella sativa</i> on ACHN and GP-293 Cell Line Morphology, Viability, and Apoptosis.","authors":"Samira Shahraki,&nbsp;Sara Hosseinian,&nbsp;Elham Shahraki,&nbsp;Mehdi Kheirandish,&nbsp;Abolfazl Khajavirad","doi":"10.4103/abr.abr_394_22","DOIUrl":"https://doi.org/10.4103/abr.abr_394_22","url":null,"abstract":"<p><strong>Background: </strong>Renal cell carcinoma (RCC) is among the top death-causing cancers. Medicinal herbs can also have beneficial effects on RCC treatment. In this project, we aimed to study the antitumor effect of dichloromethane and N-butanol fractions of hydroalcoholic extract of <i>Nigella sativa (N. sativa)</i> on the morphology, viability, and apoptosis of ACHN (human renal adenocarcinoma) and GP-293 (normal renal epithelial) cell lines.</p><p><strong>Materials and methods: </strong>In this experimental study, N-butanol and dichloromethane fractions of <i>N. sativa</i> were obtained, and ACHN and GP293 cell lines were treated with various concentrations of dichloromethane (0-100 μg/mL) and N-butanol (0-12.5 μg/mL) fractions for 24, 48, and 72 hours. Then, morphological changes, viability, and apoptosis were investigated.</p><p><strong>Results: </strong>Our results indicated that dichloromethane and N-butanol fractions cause morphological changes and significant decreases in the percentage of live cells in the ACHN cell line, in a dose- and time-dependent manner. In the GP-293 cell line, however, a lower toxicity was observed in comparison with that found for ACHN. The results of flow cytometry showed an apoptotic effect of dichloromethane and N-butanol fractions on the ACHN cell line but a higher rate of apoptosis induction for the total extract compared to the two fractions in the renal cancer cell line compared to the normal cell line.</p><p><strong>Conclusion: </strong>Our findings demonstrated that these two fractions of <i>N. sativa</i> induce inhibitory effects on the ACHN cell line morphology and viability. These effects were lower than those induced by the total extract. In addition, the two fractions caused more marked effects in the renal cancer cell line compared with the GP-293 cell line.</p>","PeriodicalId":7225,"journal":{"name":"Advanced Biomedical Research","volume":"12 ","pages":"200"},"PeriodicalIF":0.0,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/fe/71/ABR-12-200.PMC10492620.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10220083","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}